LOCUS       pGL4.32[luc2P_NF        6049 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Vector with a minimal promoter and an NF-kappa-B response element 
            for studying cell signaling using destabilized luciferase.
ACCESSION   .
VERSION     .
KEYWORDS    pGL4.32[luc2P NF-kB-RE Hygro]
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6049)
  AUTHORS   Promega
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 6049)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6049
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    33..84
                     /label=NF-kappa-B binding site
                     /bound_moiety="NF-kappa-B"
                     /note="NF-kappa-B RE"
                     /note="NF-kappa-B response element (5 copies)"
     promoter        116..147
                     /label=minP
                     /note="minimal TATA-box promoter with low basal activity"
     CDS             180..1829
                     /label=luciferase
                     /note="firefly luciferase"
     CDS             1833..1952
                     /label=hPEST
                     /note="PEST degradation sequence from mouse ornithine 
                     decarboxylase"
     polyA_signal    complement(2004..2125)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        2319..2676
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             2707..3741
                     /label=HygR
                     /note="hygromycin B phosphotransferase"
     polyA_signal    3768..3816
                     /label=poly(A) signal
                     /note="synthetic polyadenylation signal"
     rep_origin      complement(4143..4731)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4934..5791)
                     /label=AmpR
                     /note="beta-lactamase"
     polyA_signal    5896..5944
                     /label=synthetic polyadenylation signal
                     /note="synthetic polyadenylation signal"
     misc_feature    5958..6049
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from
                     the human alpha-2 globin gene"
ORIGIN
        1 ggcctaactg gccggtacct gagctcgcta gcgggaattt ccggggactt tccgggaatt
       61 tccggggact ttccgggaat ttccagatct ggcctcggcg gccaagctta gacactagag
      121 ggtatataat ggaagctcga cttccagctt ggcaatccgg tactgttggt aaagccacca
      181 tggaagatgc caaaaacatt aagaagggcc cagcgccatt ctacccactc gaagacggga
      241 ccgccggcga gcagctgcac aaagccatga agcgctacgc cctggtgccc ggcaccatcg
      301 cctttaccga cgcacatatc gaggtggaca ttacctacgc cgagtacttc gagatgagcg
      361 ttcggctggc agaagctatg aagcgctatg ggctgaatac aaaccatcgg atcgtggtgt
      421 gcagcgagaa tagcttgcag ttcttcatgc ccgtgttggg tgccctgttc atcggtgtgg
      481 ctgtggcccc agctaacgac atctacaacg agcgcgagct gctgaacagc atgggcatca
      541 gccagcccac cgtcgtattc gtgagcaaga aagggctgca aaagatcctc aacgtgcaaa
      601 agaagctacc gatcatacaa aagatcatca tcatggatag caagaccgac taccagggct
      661 tccaaagcat gtacaccttc gtgacttccc atttgccacc cggcttcaac gagtacgact
      721 tcgtgcccga gagcttcgac cgggacaaaa ccatcgccct gatcatgaac agtagtggca
      781 gtaccggatt gcccaagggc gtagccctac cgcaccgcac cgcttgtgtc cgattcagtc
      841 atgcccgcga ccccatcttc ggcaaccaga tcatccccga caccgctatc ctcagcgtgg
      901 tgccatttca ccacggcttc ggcatgttca ccacgctggg ctacttgatc tgcggctttc
      961 gggtcgtgct catgtaccgc ttcgaggagg agctattctt gcgcagcttg caagactata
     1021 agattcaatc tgccctgctg gtgcccacac tatttagctt cttcgctaag agcactctca
     1081 tcgacaagta cgacctaagc aacttgcacg agatcgccag cggcggggcg ccgctcagca
     1141 aggaggtagg tgaggccgtg gccaaacgct tccacctacc aggcatccgc cagggctacg
     1201 gcctgacaga aacaaccagc gccattctga tcacccccga aggggacgac aagcctggcg
     1261 cagtaggcaa ggtggtgccc ttcttcgagg ctaaggtggt ggacttggac accggtaaga
     1321 cactgggtgt gaaccagcgc ggcgagctgt gcgtccgtgg ccccatgatc atgagcggct
     1381 acgttaacaa ccccgaggct acaaacgctc tcatcgacaa ggacggctgg ctgcacagcg
     1441 gcgacatcgc ctactgggac gaggacgagc acttcttcat cgtggaccgg ctgaagagcc
     1501 tgatcaaata caagggctac caggtagccc cagccgaact ggagagcatc ctgctgcaac
     1561 accccaacat cttcgacgcc ggggtcgccg gcctgcccga cgacgatgcc ggcgagctgc
     1621 ccgccgcagt cgtcgtgctg gaacacggta aaaccatgac cgagaaggag atcgtggact
     1681 atgtggccag ccaggttaca accgccaaga agctgcgcgg tggtgttgtg ttcgtggacg
     1741 aggtgcctaa aggactgacc ggcaagttgg acgcccgcaa gatccgcgag attctcatta
     1801 aggccaagaa gggcggcaag atcgccgtga attctcacgg cttccctccc gaggtggagg
     1861 agcaggccgc cggcaccctg cccatgagct gcgcccagga gagcggcatg gatagacacc
     1921 ctgctgcttg cgccagcgcc aggatcaacg tctaaggccg cgactctaga gtcggggcgg
     1981 ccggccgctt cgagcagaca tgataagata cattgatgag tttggacaaa ccacaactag
     2041 aatgcagtga aaaaaatgct ttatttgtga aatttgtgat gctattgctt tatttgtaac
     2101 cattataagc tgcaataaac aagttaacaa caacaattgc attcatttta tgtttcaggt
     2161 tcagggggag gtgtgggagg ttttttaaag caagtaaaac ctctacaaat gtggtaaaat
     2221 cgataaggat ccgtttgcgt attgggcgct cttccgctga tctgcgcagc accatggcct
     2281 gaaataacct ctgaaagagg aacttggtta gctaccttct gaggcggaaa gaaccagctg
     2341 tggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccagcagg cagaagtatg
     2401 caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca
     2461 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc gcccctaact
     2521 ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta
     2581 atttttttta tttatgcaga ggccgaggcc gcctctgcct ctgagctatt ccagaagtag
     2641 tgaggaggct tttttggagg cctaggcttt tgcaaaaagc tcgattcttc tgacactagc
     2701 gccaccatga agaagcccga actcaccgct accagcgttg aaaaatttct catcgagaag
     2761 ttcgacagtg tgagcgacct gatgcagttg tcggagggcg aagagagccg agccttcagc
     2821 ttcgatgtcg gcggacgcgg ctatgtactg cgggtgaata gctgcgctga tggcttctac
     2881 aaagaccgct acgtgtaccg ccacttcgcc agcgctgcac tacccatccc cgaagtgttg
     2941 gacatcggcg agttcagcga gagcctgaca tactgcatca gtagacgcgc ccaaggcgtt
     3001 actctccaag acctccccga aacagagctg cctgctgtgt tacagcctgt cgccgaagct
     3061 atggatgcta ttgccgccgc cgacctcagt caaaccagcg gcttcggccc attcgggccc
     3121 caaggcatcg gccagtacac aacctggcgg gatttcattt gcgccattgc tgatccccat
     3181 gtctaccact ggcagaccgt gatggacgac accgtgtccg ccagcgtagc tcaagccctg
     3241 gacgaactga tgctgtgggc cgaagactgt cccgaggtgc gccacctcgt ccatgccgac
     3301 ttcggcagca acaacgtcct gaccgacaac ggccgcatca ccgccgtaat cgactggtcc
     3361 gaagctatgt tcggggacag tcagtacgag gtggccaaca tcttcttctg gcggccctgg
     3421 ctggcttgca tggagcagca gactcgctac ttcgagcgcc ggcatcccga gctggccggc
     3481 agccctcgtc tgcgagccta catgctgcgc atcggcctgg atcagctcta ccagagcctc
     3541 gtggacggca acttcgacga tgctgcctgg gctcaaggcc gctgcgatgc catcgtccgc
     3601 agcggggccg gcaccgtcgg tcgcacacaa atcgctcgcc ggagcgcagc cgtatggacc
     3661 gacggctgcg tcgaggtgct ggccgacagc ggcaaccgcc ggcccagtac acgaccgcgc
     3721 gctaaggagg taggtcgagt ttaaactcta gaaccggtca tggccgcaat aaaatatctt
     3781 tattttcatt acatctgtgt gttggttttt tgtgtgttcg aactagatgc tgtcgaccga
     3841 tgcccttgag agccttcaac ccagtcagct ccttccggtg ggcgcggggc atgactatcg
     3901 tcgccgcact tatgactgtc ttctttatca tgcaactcgt aggacaggtg ccggcagcgc
     3961 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
     4021 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
     4081 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
     4141 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
     4201 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
     4261 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
     4321 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
     4381 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
     4441 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
     4501 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
     4561 cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt
     4621 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
     4681 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
     4741 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
     4801 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
     4861 aaatcaatct aaagtatata tgagtaaact tggtctgaca gcggccgcaa atgctaaacc
     4921 actgcagtgg ttaccagtgc ttgatcagtg aggcaccgat ctcagcgatc tgcctatttc
     4981 gttcgtccat agtggcctga ctccccgtcg tgtagatcac tacgattcgt gagggcttac
     5041 catcaggccc cagcgcagca atgatgccgc gagagccgcg ttcaccggcc cccgatttgt
     5101 cagcaatgaa ccagccagca gggagggccg agcgaagaag tggtcctgct actttgtccg
     5161 cctccatcca gtctatgagc tgctgtcgtg atgctagagt aagaagttcg ccagtgagta
     5221 gtttccgaag agttgtggcc attgctactg gcatcgtggt atcacgctcg tcgttcggta
     5281 tggcttcgtt caactctggt tcccagcggt caagccgggt cacatgatca cccatattat
     5341 gaagaaatgc agtcagctcc ttagggcctc cgatcgttgt cagaagtaag ttggccgcgg
     5401 tgttgtcgct catggtaatg gcagcactac acaattctct taccgtcatg ccatccgtaa
     5461 gatgcttttc cgtgaccggc gagtactcaa ccaagtcgtt ttgtgagtag tgtatacggc
     5521 gaccaagctg ctcttgcccg gcgtctatac gggacaacac cgcgccacat agcagtactt
     5581 tgaaagtgct catcatcggg aatcgttctt cggggcggaa agactcaagg atcttgccgc
     5641 tattgagatc cagttcgata tagcccactc ttgcacccag ttgatcttca gcatctttta
     5701 ctttcaccag cgtttcgggg tgtgcaaaaa caggcaagca aaatgccgca aagaagggaa
     5761 tgagtgcgac acgaaaatgt tggatgctca tactcgtcct ttttcaatat tattgaagca
     5821 tttatcaggg ttactagtac gtctctcaag gataagtaag taatattaag gtacgggagg
     5881 tattggacag gccgcaataa aatatcttta ttttcattac atctgtgtgt tggttttttg
     5941 tgtgaatcga tagtactaac atacgctctc catcaaaaca aaacgaaaca aaacaaacta
     6001 gcaaaatagg ctgtccccag tgcaagtgca ggtgccagaa catttctct
//
