LOCUS       62056_11730        6025 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6025)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..6025
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        92..123
                     /label=minP
                     /note="minimal TATA-box promoter with low basal activity"
     CDS             156..1805
                     /codon_start=1
                     /label=luciferase
                     /note="firefly luciferase"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     CDS             1809..1928
                     /codon_start=1
                     /label=hPEST
                     /note="PEST degradation sequence from mouse ornithine 
                     decarboxylase"
                     /translation="SHGFPPEVEEQAAGTLPMSCAQESGMDRHPAACASARINV"
     polyA_signal    complement(1980..2101)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        2295..2652
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             2683..3717
                     /codon_start=1
                     /label=HygR
                     /note="hygromycin B phosphotransferase"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     EVGRV"
     polyA_signal    3744..3792
                     /label=poly(A) signal
                     /note="synthetic polyadenylation signal"
     rep_origin      complement(4119..4707)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4910..5767)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     misc_feature    5934..6025
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from
                     the human alpha-2 globin gene"
ORIGIN
        1 ggcctaactg gccggtacct gagctcgcta gctgactcat gactcatgac tcatgactca
       61 agatctggcc tcggcggcca agcttagaca ctagagggta tataatggaa gctcgacttc
      121 cagcttggca atccggtact gttggtaaag ccaccatgga agatgccaaa aacattaaga
      181 agggcccagc gccattctac ccactcgaag acgggaccgc cggcgagcag ctgcacaaag
      241 ccatgaagcg ctacgccctg gtgcccggca ccatcgcctt taccgacgca catatcgagg
      301 tggacattac ctacgccgag tacttcgaga tgagcgttcg gctggcagaa gctatgaagc
      361 gctatgggct gaatacaaac catcggatcg tggtgtgcag cgagaatagc ttgcagttct
      421 tcatgcccgt gttgggtgcc ctgttcatcg gtgtggctgt ggccccagct aacgacatct
      481 acaacgagcg cgagctgctg aacagcatgg gcatcagcca gcccaccgtc gtattcgtga
      541 gcaagaaagg gctgcaaaag atcctcaacg tgcaaaagaa gctaccgatc atacaaaaga
      601 tcatcatcat ggatagcaag accgactacc agggcttcca aagcatgtac accttcgtga
      661 cttcccattt gccacccggc ttcaacgagt acgacttcgt gcccgagagc ttcgaccggg
      721 acaaaaccat cgccctgatc atgaacagta gtggcagtac cggattgccc aagggcgtag
      781 ccctaccgca ccgcaccgct tgtgtccgat tcagtcatgc ccgcgacccc atcttcggca
      841 accagatcat ccccgacacc gctatcctca gcgtggtgcc atttcaccac ggcttcggca
      901 tgttcaccac gctgggctac ttgatctgcg gctttcgggt cgtgctcatg taccgcttcg
      961 aggaggagct attcttgcgc agcttgcaag actataagat tcaatctgcc ctgctggtgc
     1021 ccacactatt tagcttcttc gctaagagca ctctcatcga caagtacgac ctaagcaact
     1081 tgcacgagat cgccagcggc ggggcgccgc tcagcaagga ggtaggtgag gccgtggcca
     1141 aacgcttcca cctaccaggc atccgccagg gctacggcct gacagaaaca accagcgcca
     1201 ttctgatcac ccccgaaggg gacgacaagc ctggcgcagt aggcaaggtg gtgcccttct
     1261 tcgaggctaa ggtggtggac ttggacaccg gtaagacact gggtgtgaac cagcgcggcg
     1321 agctgtgcgt ccgtggcccc atgatcatga gcggctacgt taacaacccc gaggctacaa
     1381 acgctctcat cgacaaggac ggctggctgc acagcggcga catcgcctac tgggacgagg
     1441 acgagcactt cttcatcgtg gaccggctga agagcctgat caaatacaag ggctaccagg
     1501 tagccccagc cgaactggag agcatcctgc tgcaacaccc caacatcttc gacgccgggg
     1561 tcgccggcct gcccgacgac gatgccggcg agctgcccgc cgcagtcgtc gtgctggaac
     1621 acggtaaaac catgaccgag aaggagatcg tggactatgt ggccagccag gttacaaccg
     1681 ccaagaagct gcgcggtggt gttgtgttcg tggacgaggt gcctaaagga ctgaccggca
     1741 agttggacgc ccgcaagatc cgcgagattc tcattaaggc caagaagggc ggcaagatcg
     1801 ccgtgaattc tcacggcttc cctcccgagg tggaggagca ggccgccggc accctgccca
     1861 tgagctgcgc ccaggagagc ggcatggata gacaccctgc tgcttgcgcc agcgccagga
     1921 tcaacgtcta aggccgcgac tctagagtcg gggcggccgg ccgcttcgag cagacatgat
     1981 aagatacatt gatgagtttg gacaaaccac aactagaatg cagtgaaaaa aatgctttat
     2041 ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt ataagctgca ataaacaagt
     2101 taacaacaac aattgcattc attttatgtt tcaggttcag ggggaggtgt gggaggtttt
     2161 ttaaagcaag taaaacctct acaaatgtgg taaaatcgat aaggatccgt ttgcgtattg
     2221 ggcgctcttc cgctgatctg cgcagcacca tggcctgaaa taacctctga aagaggaact
     2281 tggttagcta ccttctgagg cggaaagaac cagctgtgga atgtgtgtca gttagggtgt
     2341 ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca
     2401 gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat
     2461 ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg
     2521 cccagttccg cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc
     2581 gaggccgcct ctgcctctga gctattccag aagtagtgag gaggcttttt tggaggccta
     2641 ggcttttgca aaaagctcga ttcttctgac actagcgcca ccatgaagaa gcccgaactc
     2701 accgctacca gcgttgaaaa atttctcatc gagaagttcg acagtgtgag cgacctgatg
     2761 cagttgtcgg agggcgaaga gagccgagcc ttcagcttcg atgtcggcgg acgcggctat
     2821 gtactgcggg tgaatagctg cgctgatggc ttctacaaag accgctacgt gtaccgccac
     2881 ttcgccagcg ctgcactacc catccccgaa gtgttggaca tcggcgagtt cagcgagagc
     2941 ctgacatact gcatcagtag acgcgcccaa ggcgttactc tccaagacct ccccgaaaca
     3001 gagctgcctg ctgtgttaca gcctgtcgcc gaagctatgg atgctattgc cgccgccgac
     3061 ctcagtcaaa ccagcggctt cggcccattc gggccccaag gcatcggcca gtacacaacc
     3121 tggcgggatt tcatttgcgc cattgctgat ccccatgtct accactggca gaccgtgatg
     3181 gacgacaccg tgtccgccag cgtagctcaa gccctggacg aactgatgct gtgggccgaa
     3241 gactgtcccg aggtgcgcca cctcgtccat gccgacttcg gcagcaacaa cgtcctgacc
     3301 gacaacggcc gcatcaccgc cgtaatcgac tggtccgaag ctatgttcgg ggacagtcag
     3361 tacgaggtgg ccaacatctt cttctggcgg ccctggctgg cttgcatgga gcagcagact
     3421 cgctacttcg agcgccggca tcccgagctg gccggcagcc ctcgtctgcg agcctacatg
     3481 ctgcgcatcg gcctggatca gctctaccag agcctcgtgg acggcaactt cgacgatgct
     3541 gcctgggctc aaggccgctg cgatgccatc gtccgcagcg gggccggcac cgtcggtcgc
     3601 acacaaatcg ctcgccggag cgcagccgta tggaccgacg gctgcgtcga ggtgctggcc
     3661 gacagcggca accgccggcc cagtacacga ccgcgcgcta aggaggtagg tcgagtttaa
     3721 actctagaac cggtcatggc cgcaataaaa tatctttatt ttcattacat ctgtgtgttg
     3781 gttttttgtg tgttcgaact agatgctgtc gaccgatgcc cttgagagcc ttcaacccag
     3841 tcagctcctt ccggtgggcg cggggcatga ctatcgtcgc cgcacttatg actgtcttct
     3901 ttatcatgca actcgtagga caggtgccgg cagcgctctt ccgcttcctc gctcactgac
     3961 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
     4021 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
     4081 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
     4141 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
     4201 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
     4261 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
     4321 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
     4381 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     4441 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
     4501 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga
     4561 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
     4621 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
     4681 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
     4741 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
     4801 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
     4861 taaacttggt ctgacagcgg ccgcaaatgc taaaccactg cagtggttac cagtgcttga
     4921 tcagtgaggc accgatctca gcgatctgcc tatttcgttc gtccatagtg gcctgactcc
     4981 ccgtcgtgta gatcactacg attcgtgagg gcttaccatc aggccccagc gcagcaatga
     5041 tgccgcgaga gccgcgttca ccggcccccg atttgtcagc aatgaaccag ccagcaggga
     5101 gggccgagcg aagaagtggt cctgctactt tgtccgcctc catccagtct atgagctgct
     5161 gtcgtgatgc tagagtaaga agttcgccag tgagtagttt ccgaagagtt gtggccattg
     5221 ctactggcat cgtggtatca cgctcgtcgt tcggtatggc ttcgttcaac tctggttccc
     5281 agcggtcaag ccgggtcaca tgatcaccca tattatgaag aaatgcagtc agctccttag
     5341 ggcctccgat cgttgtcaga agtaagttgg ccgcggtgtt gtcgctcatg gtaatggcag
     5401 cactacacaa ttctcttacc gtcatgccat ccgtaagatg cttttccgtg accggcgagt
     5461 actcaaccaa gtcgttttgt gagtagtgta tacggcgacc aagctgctct tgcccggcgt
     5521 ctatacggga caacaccgcg ccacatagca gtactttgaa agtgctcatc atcgggaatc
     5581 gttcttcggg gcggaaagac tcaaggatct tgccgctatt gagatccagt tcgatatagc
     5641 ccactcttgc acccagttga tcttcagcat cttttacttt caccagcgtt tcggggtgtg
     5701 caaaaacagg caagcaaaat gccgcaaaga agggaatgag tgcgacacga aaatgttgga
     5761 tgctcatact cgtccttttt caatattatt gaagcattta tcagggttac tagtacgtct
     5821 ctcaaggata agtaagtaat attaaggtac gggaggtatt ggacaggccg caataaaata
     5881 tctttatttt cattacatct gtgtgttggt tttttgtgtg aatcgatagt actaacatac
     5941 gctctccatc aaaacaaaac gaaacaaaac aaactagcaa aataggctgt ccccagtgca
     6001 agtgcaggtg ccagaacatt tctct
//
