LOCUS       62056_11720        5985 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5985)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..5985
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        52..83
                     /label=minP
                     /note="minimal TATA-box promoter with low basal activity"
     CDS             116..1765
                     /codon_start=1
                     /label=luciferase
                     /note="firefly luciferase"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     CDS             1769..1888
                     /codon_start=1
                     /label=hPEST
                     /note="PEST degradation sequence from mouse ornithine 
                     decarboxylase"
                     /translation="SHGFPPEVEEQAAGTLPMSCAQESGMDRHPAACASARINV"
     polyA_signal    complement(1940..2061)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        2255..2612
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             2643..3677
                     /codon_start=1
                     /label=HygR
                     /note="hygromycin B phosphotransferase"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     EVGRV"
     polyA_signal    3704..3752
                     /label=poly(A) signal
                     /note="synthetic polyadenylation signal"
     rep_origin      complement(4079..4667)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4870..5727)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     misc_feature    5894..5985
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from
                     the human alpha-2 globin gene"
ORIGIN
        1 ggcctaactg gccggtaccg ctctgctctg ctctgctcta agcttagaca ctagagggta
       61 tataatggaa gctcgacttc cagcttggca atccggtact gttggtaaag ccaccatgga
      121 agatgccaaa aacattaaga agggcccagc gccattctac ccactcgaag acgggaccgc
      181 cggcgagcag ctgcacaaag ccatgaagcg ctacgccctg gtgcccggca ccatcgcctt
      241 taccgacgca catatcgagg tggacattac ctacgccgag tacttcgaga tgagcgttcg
      301 gctggcagaa gctatgaagc gctatgggct gaatacaaac catcggatcg tggtgtgcag
      361 cgagaatagc ttgcagttct tcatgcccgt gttgggtgcc ctgttcatcg gtgtggctgt
      421 ggccccagct aacgacatct acaacgagcg cgagctgctg aacagcatgg gcatcagcca
      481 gcccaccgtc gtattcgtga gcaagaaagg gctgcaaaag atcctcaacg tgcaaaagaa
      541 gctaccgatc atacaaaaga tcatcatcat ggatagcaag accgactacc agggcttcca
      601 aagcatgtac accttcgtga cttcccattt gccacccggc ttcaacgagt acgacttcgt
      661 gcccgagagc ttcgaccggg acaaaaccat cgccctgatc atgaacagta gtggcagtac
      721 cggattgccc aagggcgtag ccctaccgca ccgcaccgct tgtgtccgat tcagtcatgc
      781 ccgcgacccc atcttcggca accagatcat ccccgacacc gctatcctca gcgtggtgcc
      841 atttcaccac ggcttcggca tgttcaccac gctgggctac ttgatctgcg gctttcgggt
      901 cgtgctcatg taccgcttcg aggaggagct attcttgcgc agcttgcaag actataagat
      961 tcaatctgcc ctgctggtgc ccacactatt tagcttcttc gctaagagca ctctcatcga
     1021 caagtacgac ctaagcaact tgcacgagat cgccagcggc ggggcgccgc tcagcaagga
     1081 ggtaggtgag gccgtggcca aacgcttcca cctaccaggc atccgccagg gctacggcct
     1141 gacagaaaca accagcgcca ttctgatcac ccccgaaggg gacgacaagc ctggcgcagt
     1201 aggcaaggtg gtgcccttct tcgaggctaa ggtggtggac ttggacaccg gtaagacact
     1261 gggtgtgaac cagcgcggcg agctgtgcgt ccgtggcccc atgatcatga gcggctacgt
     1321 taacaacccc gaggctacaa acgctctcat cgacaaggac ggctggctgc acagcggcga
     1381 catcgcctac tgggacgagg acgagcactt cttcatcgtg gaccggctga agagcctgat
     1441 caaatacaag ggctaccagg tagccccagc cgaactggag agcatcctgc tgcaacaccc
     1501 caacatcttc gacgccgggg tcgccggcct gcccgacgac gatgccggcg agctgcccgc
     1561 cgcagtcgtc gtgctggaac acggtaaaac catgaccgag aaggagatcg tggactatgt
     1621 ggccagccag gttacaaccg ccaagaagct gcgcggtggt gttgtgttcg tggacgaggt
     1681 gcctaaagga ctgaccggca agttggacgc ccgcaagatc cgcgagattc tcattaaggc
     1741 caagaagggc ggcaagatcg ccgtgaattc tcacggcttc cctcccgagg tggaggagca
     1801 ggccgccggc accctgccca tgagctgcgc ccaggagagc ggcatggata gacaccctgc
     1861 tgcttgcgcc agcgccagga tcaacgtcta aggccgcgac tctagagtcg gggcggccgg
     1921 ccgcttcgag cagacatgat aagatacatt gatgagtttg gacaaaccac aactagaatg
     1981 cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt
     2041 ataagctgca ataaacaagt taacaacaac aattgcattc attttatgtt tcaggttcag
     2101 ggggaggtgt gggaggtttt ttaaagcaag taaaacctct acaaatgtgg taaaatcgat
     2161 aaggatccgt ttgcgtattg ggcgctcttc cgctgatctg cgcagcacca tggcctgaaa
     2221 taacctctga aagaggaact tggttagcta ccttctgagg cggaaagaac cagctgtgga
     2281 atgtgtgtca gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa
     2341 gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca
     2401 gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc
     2461 ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt
     2521 tttttattta tgcagaggcc gaggccgcct ctgcctctga gctattccag aagtagtgag
     2581 gaggcttttt tggaggccta ggcttttgca aaaagctcga ttcttctgac actagcgcca
     2641 ccatgaagaa gcccgaactc accgctacca gcgttgaaaa atttctcatc gagaagttcg
     2701 acagtgtgag cgacctgatg cagttgtcgg agggcgaaga gagccgagcc ttcagcttcg
     2761 atgtcggcgg acgcggctat gtactgcggg tgaatagctg cgctgatggc ttctacaaag
     2821 accgctacgt gtaccgccac ttcgccagcg ctgcactacc catccccgaa gtgttggaca
     2881 tcggcgagtt cagcgagagc ctgacatact gcatcagtag acgcgcccaa ggcgttactc
     2941 tccaagacct ccccgaaaca gagctgcctg ctgtgttaca gcctgtcgcc gaagctatgg
     3001 atgctattgc cgccgccgac ctcagtcaaa ccagcggctt cggcccattc gggccccaag
     3061 gcatcggcca gtacacaacc tggcgggatt tcatttgcgc cattgctgat ccccatgtct
     3121 accactggca gaccgtgatg gacgacaccg tgtccgccag cgtagctcaa gccctggacg
     3181 aactgatgct gtgggccgaa gactgtcccg aggtgcgcca cctcgtccat gccgacttcg
     3241 gcagcaacaa cgtcctgacc gacaacggcc gcatcaccgc cgtaatcgac tggtccgaag
     3301 ctatgttcgg ggacagtcag tacgaggtgg ccaacatctt cttctggcgg ccctggctgg
     3361 cttgcatgga gcagcagact cgctacttcg agcgccggca tcccgagctg gccggcagcc
     3421 ctcgtctgcg agcctacatg ctgcgcatcg gcctggatca gctctaccag agcctcgtgg
     3481 acggcaactt cgacgatgct gcctgggctc aaggccgctg cgatgccatc gtccgcagcg
     3541 gggccggcac cgtcggtcgc acacaaatcg ctcgccggag cgcagccgta tggaccgacg
     3601 gctgcgtcga ggtgctggcc gacagcggca accgccggcc cagtacacga ccgcgcgcta
     3661 aggaggtagg tcgagtttaa actctagaac cggtcatggc cgcaataaaa tatctttatt
     3721 ttcattacat ctgtgtgttg gttttttgtg tgttcgaact agatgctgtc gaccgatgcc
     3781 cttgagagcc ttcaacccag tcagctcctt ccggtgggcg cggggcatga ctatcgtcgc
     3841 cgcacttatg actgtcttct ttatcatgca actcgtagga caggtgccgg cagcgctctt
     3901 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag
     3961 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca
     4021 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
     4081 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
     4141 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
     4201 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
     4261 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
     4321 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
     4381 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
     4441 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
     4501 actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct
     4561 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
     4621 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
     4681 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
     4741 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
     4801 caatctaaag tatatatgag taaacttggt ctgacagcgg ccgcaaatgc taaaccactg
     4861 cagtggttac cagtgcttga tcagtgaggc accgatctca gcgatctgcc tatttcgttc
     4921 gtccatagtg gcctgactcc ccgtcgtgta gatcactacg attcgtgagg gcttaccatc
     4981 aggccccagc gcagcaatga tgccgcgaga gccgcgttca ccggcccccg atttgtcagc
     5041 aatgaaccag ccagcaggga gggccgagcg aagaagtggt cctgctactt tgtccgcctc
     5101 catccagtct atgagctgct gtcgtgatgc tagagtaaga agttcgccag tgagtagttt
     5161 ccgaagagtt gtggccattg ctactggcat cgtggtatca cgctcgtcgt tcggtatggc
     5221 ttcgttcaac tctggttccc agcggtcaag ccgggtcaca tgatcaccca tattatgaag
     5281 aaatgcagtc agctccttag ggcctccgat cgttgtcaga agtaagttgg ccgcggtgtt
     5341 gtcgctcatg gtaatggcag cactacacaa ttctcttacc gtcatgccat ccgtaagatg
     5401 cttttccgtg accggcgagt actcaaccaa gtcgttttgt gagtagtgta tacggcgacc
     5461 aagctgctct tgcccggcgt ctatacggga caacaccgcg ccacatagca gtactttgaa
     5521 agtgctcatc atcgggaatc gttcttcggg gcggaaagac tcaaggatct tgccgctatt
     5581 gagatccagt tcgatatagc ccactcttgc acccagttga tcttcagcat cttttacttt
     5641 caccagcgtt tcggggtgtg caaaaacagg caagcaaaat gccgcaaaga agggaatgag
     5701 tgcgacacga aaatgttgga tgctcatact cgtccttttt caatattatt gaagcattta
     5761 tcagggttac tagtacgtct ctcaaggata agtaagtaat attaaggtac gggaggtatt
     5821 ggacaggccg caataaaata tctttatttt cattacatct gtgtgttggt tttttgtgtg
     5881 aatcgatagt actaacatac gctctccatc aaaacaaaac gaaacaaaac aaactagcaa
     5941 aataggctgt ccccagtgca agtgcaggtg ccagaacatt tctct
//
