LOCUS       pGL4.31[luc2P_GA        6107 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Vector with a promoter and a Gal4 upstream activating sequence for 
            studying cell signaling using destabilized luciferase.
ACCESSION   .
VERSION     .
KEYWORDS    pGL4.31[luc2P GAL4UAS Hygro]
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6107)
  AUTHORS   Promega
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 6107)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6107
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    31..133
                     /label=Gal4 binding site
                     /bound_moiety="Gal4"
                     /note="GAL4 UAS"
                     /note="upstream activating sequence mediating
                     Gal4-dependent induction"
     promoter        145..185
                     /note="Ad promoter"
                     /note="adenovirus major late promoter"
     CDS             238..1887
                     /label=luciferase
                     /note="firefly luciferase"
     CDS             1891..2010
                     /label=hPEST
                     /note="PEST degradation sequence from mouse ornithine 
                     decarboxylase"
     polyA_signal    complement(2062..2183)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        2377..2734
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             2765..3799
                     /label=HygR
                     /note="hygromycin B phosphotransferase"
     polyA_signal    3826..3874
                     /label=poly(A) signal
                     /note="synthetic polyadenylation signal"
     rep_origin      complement(4201..4789)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4992..5849)
                     /label=AmpR
                     /note="beta-lactamase"
     polyA_signal    5954..6002
                     /label=synthetic polyadenylation signal
                     /note="synthetic polyadenylation signal"
     misc_feature    6016..6107
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from
                     the human alpha-2 globin gene"
ORIGIN
        1 ggcctaactg gccggtaccg agtttctaga cggagtactg tcctccgagc ggagtactgt
       61 cctccgactc gagcggagta ctgtcctccg atcggagtac tgtcctccgc gaattccgga
      121 gtactgtcct ccgaagacgc tagcgggggg ctataaaagg gggtgggggc gttcgtcctc
      181 actctagatc tgcgatctaa gtaagcttgg caatccggta ctgttggtaa agccaccatg
      241 gaagatgcca aaaacattaa gaagggccca gcgccattct acccactcga agacgggacc
      301 gccggcgagc agctgcacaa agccatgaag cgctacgccc tggtgcccgg caccatcgcc
      361 tttaccgacg cacatatcga ggtggacatt acctacgccg agtacttcga gatgagcgtt
      421 cggctggcag aagctatgaa gcgctatggg ctgaatacaa accatcggat cgtggtgtgc
      481 agcgagaata gcttgcagtt cttcatgccc gtgttgggtg ccctgttcat cggtgtggct
      541 gtggccccag ctaacgacat ctacaacgag cgcgagctgc tgaacagcat gggcatcagc
      601 cagcccaccg tcgtattcgt gagcaagaaa gggctgcaaa agatcctcaa cgtgcaaaag
      661 aagctaccga tcatacaaaa gatcatcatc atggatagca agaccgacta ccagggcttc
      721 caaagcatgt acaccttcgt gacttcccat ttgccacccg gcttcaacga gtacgacttc
      781 gtgcccgaga gcttcgaccg ggacaaaacc atcgccctga tcatgaacag tagtggcagt
      841 accggattgc ccaagggcgt agccctaccg caccgcaccg cttgtgtccg attcagtcat
      901 gcccgcgacc ccatcttcgg caaccagatc atccccgaca ccgctatcct cagcgtggtg
      961 ccatttcacc acggcttcgg catgttcacc acgctgggct acttgatctg cggctttcgg
     1021 gtcgtgctca tgtaccgctt cgaggaggag ctattcttgc gcagcttgca agactataag
     1081 attcaatctg ccctgctggt gcccacacta tttagcttct tcgctaagag cactctcatc
     1141 gacaagtacg acctaagcaa cttgcacgag atcgccagcg gcggggcgcc gctcagcaag
     1201 gaggtaggtg aggccgtggc caaacgcttc cacctaccag gcatccgcca gggctacggc
     1261 ctgacagaaa caaccagcgc cattctgatc acccccgaag gggacgacaa gcctggcgca
     1321 gtaggcaagg tggtgccctt cttcgaggct aaggtggtgg acttggacac cggtaagaca
     1381 ctgggtgtga accagcgcgg cgagctgtgc gtccgtggcc ccatgatcat gagcggctac
     1441 gttaacaacc ccgaggctac aaacgctctc atcgacaagg acggctggct gcacagcggc
     1501 gacatcgcct actgggacga ggacgagcac ttcttcatcg tggaccggct gaagagcctg
     1561 atcaaataca agggctacca ggtagcccca gccgaactgg agagcatcct gctgcaacac
     1621 cccaacatct tcgacgccgg ggtcgccggc ctgcccgacg acgatgccgg cgagctgccc
     1681 gccgcagtcg tcgtgctgga acacggtaaa accatgaccg agaaggagat cgtggactat
     1741 gtggccagcc aggttacaac cgccaagaag ctgcgcggtg gtgttgtgtt cgtggacgag
     1801 gtgcctaaag gactgaccgg caagttggac gcccgcaaga tccgcgagat tctcattaag
     1861 gccaagaagg gcggcaagat cgccgtgaat tctcacggct tccctcccga ggtggaggag
     1921 caggccgccg gcaccctgcc catgagctgc gcccaggaga gcggcatgga tagacaccct
     1981 gctgcttgcg ccagcgccag gatcaacgtc taaggccgcg actctagagt cggggcggcc
     2041 ggccgcttcg agcagacatg ataagataca ttgatgagtt tggacaaacc acaactagaa
     2101 tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta tttgtaacca
     2161 ttataagctg caataaacaa gttaacaaca acaattgcat tcattttatg tttcaggttc
     2221 agggggaggt gtgggaggtt ttttaaagca agtaaaacct ctacaaatgt ggtaaaatcg
     2281 ataaggatcc gtttgcgtat tgggcgctct tccgctgatc tgcgcagcac catggcctga
     2341 aataacctct gaaagaggaa cttggttagc taccttctga ggcggaaaga accagctgtg
     2401 gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
     2461 aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg
     2521 cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc
     2581 gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat
     2641 tttttttatt tatgcagagg ccgaggccgc ctctgcctct gagctattcc agaagtagtg
     2701 aggaggcttt tttggaggcc taggcttttg caaaaagctc gattcttctg acactagcgc
     2761 caccatgaag aagcccgaac tcaccgctac cagcgttgaa aaatttctca tcgagaagtt
     2821 cgacagtgtg agcgacctga tgcagttgtc ggagggcgaa gagagccgag ccttcagctt
     2881 cgatgtcggc ggacgcggct atgtactgcg ggtgaatagc tgcgctgatg gcttctacaa
     2941 agaccgctac gtgtaccgcc acttcgccag cgctgcacta cccatccccg aagtgttgga
     3001 catcggcgag ttcagcgaga gcctgacata ctgcatcagt agacgcgccc aaggcgttac
     3061 tctccaagac ctccccgaaa cagagctgcc tgctgtgtta cagcctgtcg ccgaagctat
     3121 ggatgctatt gccgccgccg acctcagtca aaccagcggc ttcggcccat tcgggcccca
     3181 aggcatcggc cagtacacaa cctggcggga tttcatttgc gccattgctg atccccatgt
     3241 ctaccactgg cagaccgtga tggacgacac cgtgtccgcc agcgtagctc aagccctgga
     3301 cgaactgatg ctgtgggccg aagactgtcc cgaggtgcgc cacctcgtcc atgccgactt
     3361 cggcagcaac aacgtcctga ccgacaacgg ccgcatcacc gccgtaatcg actggtccga
     3421 agctatgttc ggggacagtc agtacgaggt ggccaacatc ttcttctggc ggccctggct
     3481 ggcttgcatg gagcagcaga ctcgctactt cgagcgccgg catcccgagc tggccggcag
     3541 ccctcgtctg cgagcctaca tgctgcgcat cggcctggat cagctctacc agagcctcgt
     3601 ggacggcaac ttcgacgatg ctgcctgggc tcaaggccgc tgcgatgcca tcgtccgcag
     3661 cggggccggc accgtcggtc gcacacaaat cgctcgccgg agcgcagccg tatggaccga
     3721 cggctgcgtc gaggtgctgg ccgacagcgg caaccgccgg cccagtacac gaccgcgcgc
     3781 taaggaggta ggtcgagttt aaactctaga accggtcatg gccgcaataa aatatcttta
     3841 ttttcattac atctgtgtgt tggttttttg tgtgttcgaa ctagatgctg tcgaccgatg
     3901 cccttgagag ccttcaaccc agtcagctcc ttccggtggg cgcggggcat gactatcgtc
     3961 gccgcactta tgactgtctt ctttatcatg caactcgtag gacaggtgcc ggcagcgctc
     4021 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
     4081 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
     4141 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     4201 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     4261 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     4321 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     4381 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     4441 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     4501 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     4561 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     4621 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
     4681 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
     4741 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     4801 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
     4861 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
     4921 atcaatctaa agtatatatg agtaaacttg gtctgacagc ggccgcaaat gctaaaccac
     4981 tgcagtggtt accagtgctt gatcagtgag gcaccgatct cagcgatctg cctatttcgt
     5041 tcgtccatag tggcctgact ccccgtcgtg tagatcacta cgattcgtga gggcttacca
     5101 tcaggcccca gcgcagcaat gatgccgcga gagccgcgtt caccggcccc cgatttgtca
     5161 gcaatgaacc agccagcagg gagggccgag cgaagaagtg gtcctgctac tttgtccgcc
     5221 tccatccagt ctatgagctg ctgtcgtgat gctagagtaa gaagttcgcc agtgagtagt
     5281 ttccgaagag ttgtggccat tgctactggc atcgtggtat cacgctcgtc gttcggtatg
     5341 gcttcgttca actctggttc ccagcggtca agccgggtca catgatcacc catattatga
     5401 agaaatgcag tcagctcctt agggcctccg atcgttgtca gaagtaagtt ggccgcggtg
     5461 ttgtcgctca tggtaatggc agcactacac aattctctta ccgtcatgcc atccgtaaga
     5521 tgcttttccg tgaccggcga gtactcaacc aagtcgtttt gtgagtagtg tatacggcga
     5581 ccaagctgct cttgcccggc gtctatacgg gacaacaccg cgccacatag cagtactttg
     5641 aaagtgctca tcatcgggaa tcgttcttcg gggcggaaag actcaaggat cttgccgcta
     5701 ttgagatcca gttcgatata gcccactctt gcacccagtt gatcttcagc atcttttact
     5761 ttcaccagcg tttcggggtg tgcaaaaaca ggcaagcaaa atgccgcaaa gaagggaatg
     5821 agtgcgacac gaaaatgttg gatgctcata ctcgtccttt ttcaatatta ttgaagcatt
     5881 tatcagggtt actagtacgt ctctcaagga taagtaagta atattaaggt acgggaggta
     5941 ttggacaggc cgcaataaaa tatctttatt ttcattacat ctgtgtgttg gttttttgtg
     6001 tgaatcgata gtactaacat acgctctcca tcaaaacaaa acgaaacaaa acaaactagc
     6061 aaaataggct gtccccagtg caagtgcagg tgccagaaca tttctct
//
