LOCUS       pGL4.30[luc2P_NF        6097 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Vector with a minimal promoter and an NFAT response element for 
            studying cell signaling using destabilized luciferase.
ACCESSION   .
VERSION     .
KEYWORDS    pGL4.30[luc2P NFAT-RE Hygro]
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6097)
  AUTHORS   Promega
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 6097)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6097
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    33..62
                     /label=NFAT binding site
                     /note="NFAT binding site from the human interleukin 2
                     (IL-2) gene (Mattila et al., 1990)"
     promoter        135..166
                     /label=minP
                     /note="minimal TATA-box promoter with low basal activity"
     CDS             228..1877
                     /label=luciferase
                     /note="firefly luciferase"
     CDS             1881..2000
                     /label=hPEST
                     /note="PEST degradation sequence from mouse ornithine 
                     decarboxylase"
     polyA_signal    complement(2052..2173)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        2367..2724
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             2755..3789
                     /label=HygR
                     /note="hygromycin B phosphotransferase"
     polyA_signal    3816..3864
                     /label=poly(A) signal
                     /note="synthetic polyadenylation signal"
     rep_origin      complement(4191..4779)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4982..5839)
                     /label=AmpR
                     /note="beta-lactamase"
     polyA_signal    5944..5992
                     /label=synthetic polyadenylation signal
                     /note="synthetic polyadenylation signal"
     misc_feature    6006..6097
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from
                     the human alpha-2 globin gene"
ORIGIN
        1 ggcctaactg gccggtacct gagctcgcta gcggaggaaa aactgtttca tacagaaggc
       61 gtggaggaaa aactgtttca tacagaaggc gtggaggaaa aactgtttca tacagaaggc
      121 gtagatctag actctagagg gtatataatg gaagctcgaa ttccagcttg gcattccggt
      181 actgttggta aaaagcttgg caatccggta ctgttggtaa agccaccatg gaagatgcca
      241 aaaacattaa gaagggccca gcgccattct acccactcga agacgggacc gccggcgagc
      301 agctgcacaa agccatgaag cgctacgccc tggtgcccgg caccatcgcc tttaccgacg
      361 cacatatcga ggtggacatt acctacgccg agtacttcga gatgagcgtt cggctggcag
      421 aagctatgaa gcgctatggg ctgaatacaa accatcggat cgtggtgtgc agcgagaata
      481 gcttgcagtt cttcatgccc gtgttgggtg ccctgttcat cggtgtggct gtggccccag
      541 ctaacgacat ctacaacgag cgcgagctgc tgaacagcat gggcatcagc cagcccaccg
      601 tcgtattcgt gagcaagaaa gggctgcaaa agatcctcaa cgtgcaaaag aagctaccga
      661 tcatacaaaa gatcatcatc atggatagca agaccgacta ccagggcttc caaagcatgt
      721 acaccttcgt gacttcccat ttgccacccg gcttcaacga gtacgacttc gtgcccgaga
      781 gcttcgaccg ggacaaaacc atcgccctga tcatgaacag tagtggcagt accggattgc
      841 ccaagggcgt agccctaccg caccgcaccg cttgtgtccg attcagtcat gcccgcgacc
      901 ccatcttcgg caaccagatc atccccgaca ccgctatcct cagcgtggtg ccatttcacc
      961 acggcttcgg catgttcacc acgctgggct acttgatctg cggctttcgg gtcgtgctca
     1021 tgtaccgctt cgaggaggag ctattcttgc gcagcttgca agactataag attcaatctg
     1081 ccctgctggt gcccacacta tttagcttct tcgctaagag cactctcatc gacaagtacg
     1141 acctaagcaa cttgcacgag atcgccagcg gcggggcgcc gctcagcaag gaggtaggtg
     1201 aggccgtggc caaacgcttc cacctaccag gcatccgcca gggctacggc ctgacagaaa
     1261 caaccagcgc cattctgatc acccccgaag gggacgacaa gcctggcgca gtaggcaagg
     1321 tggtgccctt cttcgaggct aaggtggtgg acttggacac cggtaagaca ctgggtgtga
     1381 accagcgcgg cgagctgtgc gtccgtggcc ccatgatcat gagcggctac gttaacaacc
     1441 ccgaggctac aaacgctctc atcgacaagg acggctggct gcacagcggc gacatcgcct
     1501 actgggacga ggacgagcac ttcttcatcg tggaccggct gaagagcctg atcaaataca
     1561 agggctacca ggtagcccca gccgaactgg agagcatcct gctgcaacac cccaacatct
     1621 tcgacgccgg ggtcgccggc ctgcccgacg acgatgccgg cgagctgccc gccgcagtcg
     1681 tcgtgctgga acacggtaaa accatgaccg agaaggagat cgtggactat gtggccagcc
     1741 aggttacaac cgccaagaag ctgcgcggtg gtgttgtgtt cgtggacgag gtgcctaaag
     1801 gactgaccgg caagttggac gcccgcaaga tccgcgagat tctcattaag gccaagaagg
     1861 gcggcaagat cgccgtgaat tctcacggct tccctcccga ggtggaggag caggccgccg
     1921 gcaccctgcc catgagctgc gcccaggaga gcggcatgga tagacaccct gctgcttgcg
     1981 ccagcgccag gatcaacgtc taaggccgcg actctagagt cggggcggcc ggccgcttcg
     2041 agcagacatg ataagataca ttgatgagtt tggacaaacc acaactagaa tgcagtgaaa
     2101 aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta tttgtaacca ttataagctg
     2161 caataaacaa gttaacaaca acaattgcat tcattttatg tttcaggttc agggggaggt
     2221 gtgggaggtt ttttaaagca agtaaaacct ctacaaatgt ggtaaaatcg ataaggatcc
     2281 gtttgcgtat tgggcgctct tccgctgatc tgcgcagcac catggcctga aataacctct
     2341 gaaagaggaa cttggttagc taccttctga ggcggaaaga accagctgtg gaatgtgtgt
     2401 cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat
     2461 ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg
     2521 caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg
     2581 cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt
     2641 tatgcagagg ccgaggccgc ctctgcctct gagctattcc agaagtagtg aggaggcttt
     2701 tttggaggcc taggcttttg caaaaagctc gattcttctg acactagcgc caccatgaag
     2761 aagcccgaac tcaccgctac cagcgttgaa aaatttctca tcgagaagtt cgacagtgtg
     2821 agcgacctga tgcagttgtc ggagggcgaa gagagccgag ccttcagctt cgatgtcggc
     2881 ggacgcggct atgtactgcg ggtgaatagc tgcgctgatg gcttctacaa agaccgctac
     2941 gtgtaccgcc acttcgccag cgctgcacta cccatccccg aagtgttgga catcggcgag
     3001 ttcagcgaga gcctgacata ctgcatcagt agacgcgccc aaggcgttac tctccaagac
     3061 ctccccgaaa cagagctgcc tgctgtgtta cagcctgtcg ccgaagctat ggatgctatt
     3121 gccgccgccg acctcagtca aaccagcggc ttcggcccat tcgggcccca aggcatcggc
     3181 cagtacacaa cctggcggga tttcatttgc gccattgctg atccccatgt ctaccactgg
     3241 cagaccgtga tggacgacac cgtgtccgcc agcgtagctc aagccctgga cgaactgatg
     3301 ctgtgggccg aagactgtcc cgaggtgcgc cacctcgtcc atgccgactt cggcagcaac
     3361 aacgtcctga ccgacaacgg ccgcatcacc gccgtaatcg actggtccga agctatgttc
     3421 ggggacagtc agtacgaggt ggccaacatc ttcttctggc ggccctggct ggcttgcatg
     3481 gagcagcaga ctcgctactt cgagcgccgg catcccgagc tggccggcag ccctcgtctg
     3541 cgagcctaca tgctgcgcat cggcctggat cagctctacc agagcctcgt ggacggcaac
     3601 ttcgacgatg ctgcctgggc tcaaggccgc tgcgatgcca tcgtccgcag cggggccggc
     3661 accgtcggtc gcacacaaat cgctcgccgg agcgcagccg tatggaccga cggctgcgtc
     3721 gaggtgctgg ccgacagcgg caaccgccgg cccagtacac gaccgcgcgc taaggaggta
     3781 ggtcgagttt aaactctaga accggtcatg gccgcaataa aatatcttta ttttcattac
     3841 atctgtgtgt tggttttttg tgtgttcgaa ctagatgctg tcgaccgatg cccttgagag
     3901 ccttcaaccc agtcagctcc ttccggtggg cgcggggcat gactatcgtc gccgcactta
     3961 tgactgtctt ctttatcatg caactcgtag gacaggtgcc ggcagcgctc ttccgcttcc
     4021 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
     4081 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
     4141 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
     4201 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
     4261 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     4321 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     4381 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     4441 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
     4501 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
     4561 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
     4621 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
     4681 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
     4741 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
     4801 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
     4861 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
     4921 agtatatatg agtaaacttg gtctgacagc ggccgcaaat gctaaaccac tgcagtggtt
     4981 accagtgctt gatcagtgag gcaccgatct cagcgatctg cctatttcgt tcgtccatag
     5041 tggcctgact ccccgtcgtg tagatcacta cgattcgtga gggcttacca tcaggcccca
     5101 gcgcagcaat gatgccgcga gagccgcgtt caccggcccc cgatttgtca gcaatgaacc
     5161 agccagcagg gagggccgag cgaagaagtg gtcctgctac tttgtccgcc tccatccagt
     5221 ctatgagctg ctgtcgtgat gctagagtaa gaagttcgcc agtgagtagt ttccgaagag
     5281 ttgtggccat tgctactggc atcgtggtat cacgctcgtc gttcggtatg gcttcgttca
     5341 actctggttc ccagcggtca agccgggtca catgatcacc catattatga agaaatgcag
     5401 tcagctcctt agggcctccg atcgttgtca gaagtaagtt ggccgcggtg ttgtcgctca
     5461 tggtaatggc agcactacac aattctctta ccgtcatgcc atccgtaaga tgcttttccg
     5521 tgaccggcga gtactcaacc aagtcgtttt gtgagtagtg tatacggcga ccaagctgct
     5581 cttgcccggc gtctatacgg gacaacaccg cgccacatag cagtactttg aaagtgctca
     5641 tcatcgggaa tcgttcttcg gggcggaaag actcaaggat cttgccgcta ttgagatcca
     5701 gttcgatata gcccactctt gcacccagtt gatcttcagc atcttttact ttcaccagcg
     5761 tttcggggtg tgcaaaaaca ggcaagcaaa atgccgcaaa gaagggaatg agtgcgacac
     5821 gaaaatgttg gatgctcata ctcgtccttt ttcaatatta ttgaagcatt tatcagggtt
     5881 actagtacgt ctctcaagga taagtaagta atattaaggt acgggaggta ttggacaggc
     5941 cgcaataaaa tatctttatt ttcattacat ctgtgtgttg gttttttgtg tgaatcgata
     6001 gtactaacat acgctctcca tcaaaacaaa acgaaacaaa acaaactagc aaaataggct
     6061 gtccccagtg caagtgcagg tgccagaaca tttctct
//
