LOCUS       62056_11715        7606 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7606)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..7606
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        20..323
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        324..527
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     CDS             557..1273
                     /codon_start=1
                     /label=EGFP
                     /note="enhanced GFP"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     polyA_signal    1399..1520
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        1673..1704
                     /label=minP
                     /note="minimal TATA-box promoter with low basal activity"
     CDS             1737..3386
                     /codon_start=1
                     /label=luciferase
                     /note="firefly luciferase"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     CDS             3390..3509
                     /codon_start=1
                     /label=hPEST
                     /note="PEST degradation sequence from mouse ornithine 
                     decarboxylase"
                     /translation="SHGFPPEVEEQAAGTLPMSCAQESGMDRHPAACASARINV"
     polyA_signal    complement(3561..3682)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        3876..4233
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             4264..5298
                     /codon_start=1
                     /label=HygR
                     /note="hygromycin B phosphotransferase"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     EVGRV"
     polyA_signal    5325..5373
                     /label=poly(A) signal
                     /note="synthetic polyadenylation signal"
     rep_origin      complement(5700..6288)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(6491..7348)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     misc_feature    7515..7606
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from
                     the human alpha-2 globin gene"
ORIGIN
        1 ggcctaactg gccggtaccc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
       61 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
      121 ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac
      181 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg
      241 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg
      301 tattagtcat cgctattacc atggtgatgc ggttttggca gtacatcaat gggcgtggat
      361 agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt
      421 tttggcacca aaatcaacgg gactttccaa aatgtcgtaa caactccgcc ccattgacgc
      481 aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctggt ttagtgaacc
      541 gtcagatccc gccaccatgg tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat
      601 cctggtcgag ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga
      661 gggcgatgcc acctacggca agctgaccct gaagttcatc tgcaccaccg gcaagctgcc
      721 cgtgccctgg cccaccctcg tgaccaccct gacctacggc gtgcagtgct tcagccgcta
      781 ccccgaccac atgaagcagc acgacttctt caagtccgcc atgcccgaag gctacgtcca
      841 ggagcgcacc atcttcttca aggacgacgg caactacaag acccgcgccg aggtgaagtt
      901 cgagggcgac accctggtga accgcatcga gctgaagggc atcgacttca aggaggacgg
      961 caacatcctg gggcacaagc tggagtacaa ctacaacagc cacaacgtct atatcatggc
     1021 cgacaagcag aagaacggca tcaaggtgaa cttcaagatc cgccacaaca tcgaggacgg
     1081 cagcgtgcag ctcgccgacc actaccagca gaacaccccc atcggcgacg gccccgtgct
     1141 gctgcccgac aaccactacc tgagcaccca gtccgccctg agcaaagacc ccaacgagaa
     1201 gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc gggatcactc tcggcatgga
     1261 cgagctgtac aagtaaagcg gccgcgactc tagatcataa tcagccatac cacatttgta
     1321 gaggttttac ttgctttaaa aaacctccca cacctccccc tgaacctgaa acataaaatg
     1381 aatgcaattg ttgttgttaa cttgtttatt gcagcttata atggttacaa ataaagcaat
     1441 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc
     1501 aaactcatca atgtatctta gctagcagga tgtccatatt aggacatcta ggatgtccat
     1561 attaggacat ctaggatgtc catattagga catctaggat gtccatatta ggacatctag
     1621 gatgtccata ttaggacatc tagatctggc ctcggcggcc aagcttagac actagagggt
     1681 atataatgga agctcgactt ccagcttggc aatccggtac tgttggtaaa gccaccatgg
     1741 aagatgccaa aaacattaag aagggcccag cgccattcta cccactcgaa gacgggaccg
     1801 ccggcgagca gctgcacaaa gccatgaagc gctacgccct ggtgcccggc accatcgcct
     1861 ttaccgacgc acatatcgag gtggacatta cctacgccga gtacttcgag atgagcgttc
     1921 ggctggcaga agctatgaag cgctatgggc tgaatacaaa ccatcggatc gtggtgtgca
     1981 gcgagaatag cttgcagttc ttcatgcccg tgttgggtgc cctgttcatc ggtgtggctg
     2041 tggccccagc taacgacatc tacaacgagc gcgagctgct gaacagcatg ggcatcagcc
     2101 agcccaccgt cgtattcgtg agcaagaaag ggctgcaaaa gatcctcaac gtgcaaaaga
     2161 agctaccgat catacaaaag atcatcatca tggatagcaa gaccgactac cagggcttcc
     2221 aaagcatgta caccttcgtg acttcccatt tgccacccgg cttcaacgag tacgacttcg
     2281 tgcccgagag cttcgaccgg gacaaaacca tcgccctgat catgaacagt agtggcagta
     2341 ccggattgcc caagggcgta gccctaccgc accgcaccgc ttgtgtccga ttcagtcatg
     2401 cccgcgaccc catcttcggc aaccagatca tccccgacac cgctatcctc agcgtggtgc
     2461 catttcacca cggcttcggc atgttcacca cgctgggcta cttgatctgc ggctttcggg
     2521 tcgtgctcat gtaccgcttc gaggaggagc tattcttgcg cagcttgcaa gactataaga
     2581 ttcaatctgc cctgctggtg cccacactat ttagcttctt cgctaagagc actctcatcg
     2641 acaagtacga cctaagcaac ttgcacgaga tcgccagcgg cggggcgccg ctcagcaagg
     2701 aggtaggtga ggccgtggcc aaacgcttcc acctaccagg catccgccag ggctacggcc
     2761 tgacagaaac aaccagcgcc attctgatca cccccgaagg ggacgacaag cctggcgcag
     2821 taggcaaggt ggtgcccttc ttcgaggcta aggtggtgga cttggacacc ggtaagacac
     2881 tgggtgtgaa ccagcgcggc gagctgtgcg tccgtggccc catgatcatg agcggctacg
     2941 ttaacaaccc cgaggctaca aacgctctca tcgacaagga cggctggctg cacagcggcg
     3001 acatcgccta ctgggacgag gacgagcact tcttcatcgt ggaccggctg aagagcctga
     3061 tcaaatacaa gggctaccag gtagccccag ccgaactgga gagcatcctg ctgcaacacc
     3121 ccaacatctt cgacgccggg gtcgccggcc tgcccgacga cgatgccggc gagctgcccg
     3181 ccgcagtcgt cgtgctggaa cacggtaaaa ccatgaccga gaaggagatc gtggactatg
     3241 tggccagcca ggttacaacc gccaagaagc tgcgcggtgg tgttgtgttc gtggacgagg
     3301 tgcctaaagg actgaccggc aagttggacg cccgcaagat ccgcgagatt ctcattaagg
     3361 ccaagaaggg cggcaagatc gccgtgaatt ctcacggctt ccctcccgag gtggaggagc
     3421 aggccgccgg caccctgccc atgagctgcg cccaggagag cggcatggat agacaccctg
     3481 ctgcttgcgc cagcgccagg atcaacgtct aaggccgcga ctctagagtc ggggcggccg
     3541 gccgcttcga gcagacatga taagatacat tgatgagttt ggacaaacca caactagaat
     3601 gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct attgctttat ttgtaaccat
     3661 tataagctgc aataaacaag ttaacaacaa caattgcatt cattttatgt ttcaggttca
     3721 gggggaggtg tgggaggttt tttaaagcaa gtaaaacctc tacaaatgtg gtaaaatcga
     3781 taaggatccg tttgcgtatt gggcgctctt ccgctgatct gcgcagcacc atggcctgaa
     3841 ataacctctg aaagaggaac ttggttagct accttctgag gcggaaagaa ccagctgtgg
     3901 aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa
     3961 agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc
     4021 agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg
     4081 cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt
     4141 ttttttattt atgcagaggc cgaggccgcc tctgcctctg agctattcca gaagtagtga
     4201 ggaggctttt ttggaggcct aggcttttgc aaaaagctcg attcttctga cactagcgcc
     4261 accatgaaga agcccgaact caccgctacc agcgttgaaa aatttctcat cgagaagttc
     4321 gacagtgtga gcgacctgat gcagttgtcg gagggcgaag agagccgagc cttcagcttc
     4381 gatgtcggcg gacgcggcta tgtactgcgg gtgaatagct gcgctgatgg cttctacaaa
     4441 gaccgctacg tgtaccgcca cttcgccagc gctgcactac ccatccccga agtgttggac
     4501 atcggcgagt tcagcgagag cctgacatac tgcatcagta gacgcgccca aggcgttact
     4561 ctccaagacc tccccgaaac agagctgcct gctgtgttac agcctgtcgc cgaagctatg
     4621 gatgctattg ccgccgccga cctcagtcaa accagcggct tcggcccatt cgggccccaa
     4681 ggcatcggcc agtacacaac ctggcgggat ttcatttgcg ccattgctga tccccatgtc
     4741 taccactggc agaccgtgat ggacgacacc gtgtccgcca gcgtagctca agccctggac
     4801 gaactgatgc tgtgggccga agactgtccc gaggtgcgcc acctcgtcca tgccgacttc
     4861 ggcagcaaca acgtcctgac cgacaacggc cgcatcaccg ccgtaatcga ctggtccgaa
     4921 gctatgttcg gggacagtca gtacgaggtg gccaacatct tcttctggcg gccctggctg
     4981 gcttgcatgg agcagcagac tcgctacttc gagcgccggc atcccgagct ggccggcagc
     5041 cctcgtctgc gagcctacat gctgcgcatc ggcctggatc agctctacca gagcctcgtg
     5101 gacggcaact tcgacgatgc tgcctgggct caaggccgct gcgatgccat cgtccgcagc
     5161 ggggccggca ccgtcggtcg cacacaaatc gctcgccgga gcgcagccgt atggaccgac
     5221 ggctgcgtcg aggtgctggc cgacagcggc aaccgccggc ccagtacacg accgcgcgct
     5281 aaggaggtag gtcgagttta aactctagaa ccggtcatgg ccgcaataaa atatctttat
     5341 tttcattaca tctgtgtgtt ggttttttgt gtgttcgaac tagatgctgt cgaccgatgc
     5401 ccttgagagc cttcaaccca gtcagctcct tccggtgggc gcggggcatg actatcgtcg
     5461 ccgcacttat gactgtcttc tttatcatgc aactcgtagg acaggtgccg gcagcgctct
     5521 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
     5581 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
     5641 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
     5701 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
     5761 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
     5821 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
     5881 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
     5941 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
     6001 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
     6061 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
     6121 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc
     6181 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
     6241 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
     6301 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
     6361 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
     6421 tcaatctaaa gtatatatga gtaaacttgg tctgacagcg gccgcaaatg ctaaaccact
     6481 gcagtggtta ccagtgcttg atcagtgagg caccgatctc agcgatctgc ctatttcgtt
     6541 cgtccatagt ggcctgactc cccgtcgtgt agatcactac gattcgtgag ggcttaccat
     6601 caggccccag cgcagcaatg atgccgcgag agccgcgttc accggccccc gatttgtcag
     6661 caatgaacca gccagcaggg agggccgagc gaagaagtgg tcctgctact ttgtccgcct
     6721 ccatccagtc tatgagctgc tgtcgtgatg ctagagtaag aagttcgcca gtgagtagtt
     6781 tccgaagagt tgtggccatt gctactggca tcgtggtatc acgctcgtcg ttcggtatgg
     6841 cttcgttcaa ctctggttcc cagcggtcaa gccgggtcac atgatcaccc atattatgaa
     6901 gaaatgcagt cagctcctta gggcctccga tcgttgtcag aagtaagttg gccgcggtgt
     6961 tgtcgctcat ggtaatggca gcactacaca attctcttac cgtcatgcca tccgtaagat
     7021 gcttttccgt gaccggcgag tactcaacca agtcgttttg tgagtagtgt atacggcgac
     7081 caagctgctc ttgcccggcg tctatacggg acaacaccgc gccacatagc agtactttga
     7141 aagtgctcat catcgggaat cgttcttcgg ggcggaaaga ctcaaggatc ttgccgctat
     7201 tgagatccag ttcgatatag cccactcttg cacccagttg atcttcagca tcttttactt
     7261 tcaccagcgt ttcggggtgt gcaaaaacag gcaagcaaaa tgccgcaaag aagggaatga
     7321 gtgcgacacg aaaatgttgg atgctcatac tcgtcctttt tcaatattat tgaagcattt
     7381 atcagggtta ctagtacgtc tctcaaggat aagtaagtaa tattaaggta cgggaggtat
     7441 tggacaggcc gcaataaaat atctttattt tcattacatc tgtgtgttgg ttttttgtgt
     7501 gaatcgatag tactaacata cgctctccat caaaacaaaa cgaaacaaaa caaactagca
     7561 aaataggctg tccccagtgc aagtgcaggt gccagaacat ttctct
//
