LOCUS       pGL4.26[luc2_min        5882 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Vector with a minimal promoter for measuring the activity of 
            transcriptional response elements using a luciferase assay.
ACCESSION   .
VERSION     .
KEYWORDS    pGL4.26[luc2 minP Hygro]
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5882)
  AUTHORS   Promega
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 5882)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5882
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..70
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     promoter        77..108
                     /label=minP
                     /note="minimal TATA-box promoter with low basal activity"
     CDS             141..1790
                     /label=luciferase
                     /note="firefly luciferase"
     polyA_signal    complement(1837..1958)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        2152..2509
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             2540..3574
                     /label=HygR
                     /note="hygromycin B phosphotransferase"
     polyA_signal    3601..3649
                     /label=poly(A) signal
                     /note="synthetic polyadenylation signal"
     rep_origin      complement(3976..4564)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4767..5624)
                     /label=AmpR
                     /note="beta-lactamase"
     polyA_signal    5729..5777
                     /label=synthetic polyadenylation signal
                     /note="synthetic polyadenylation signal"
     misc_feature    5791..5882
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from
                     the human alpha-2 globin gene"
ORIGIN
        1 ggcctaactg gccggtacct gagctcgcta gcctcgagga tatcaagatc tggcctcggc
       61 ggccaagctt agacactaga gggtatataa tggaagctcg acttccagct tggcaatccg
      121 gtactgttgg taaagccacc atggaagatg ccaaaaacat taagaagggc ccagcgccat
      181 tctacccact cgaagacggg accgccggcg agcagctgca caaagccatg aagcgctacg
      241 ccctggtgcc cggcaccatc gcctttaccg acgcacatat cgaggtggac attacctacg
      301 ccgagtactt cgagatgagc gttcggctgg cagaagctat gaagcgctat gggctgaata
      361 caaaccatcg gatcgtggtg tgcagcgaga atagcttgca gttcttcatg cccgtgttgg
      421 gtgccctgtt catcggtgtg gctgtggccc cagctaacga catctacaac gagcgcgagc
      481 tgctgaacag catgggcatc agccagccca ccgtcgtatt cgtgagcaag aaagggctgc
      541 aaaagatcct caacgtgcaa aagaagctac cgatcataca aaagatcatc atcatggata
      601 gcaagaccga ctaccagggc ttccaaagca tgtacacctt cgtgacttcc catttgccac
      661 ccggcttcaa cgagtacgac ttcgtgcccg agagcttcga ccgggacaaa accatcgccc
      721 tgatcatgaa cagtagtggc agtaccggat tgcccaaggg cgtagcccta ccgcaccgca
      781 ccgcttgtgt ccgattcagt catgcccgcg accccatctt cggcaaccag atcatccccg
      841 acaccgctat cctcagcgtg gtgccatttc accacggctt cggcatgttc accacgctgg
      901 gctacttgat ctgcggcttt cgggtcgtgc tcatgtaccg cttcgaggag gagctattct
      961 tgcgcagctt gcaagactat aagattcaat ctgccctgct ggtgcccaca ctatttagct
     1021 tcttcgctaa gagcactctc atcgacaagt acgacctaag caacttgcac gagatcgcca
     1081 gcggcggggc gccgctcagc aaggaggtag gtgaggccgt ggccaaacgc ttccacctac
     1141 caggcatccg ccagggctac ggcctgacag aaacaaccag cgccattctg atcacccccg
     1201 aaggggacga caagcctggc gcagtaggca aggtggtgcc cttcttcgag gctaaggtgg
     1261 tggacttgga caccggtaag acactgggtg tgaaccagcg cggcgagctg tgcgtccgtg
     1321 gccccatgat catgagcggc tacgttaaca accccgaggc tacaaacgct ctcatcgaca
     1381 aggacggctg gctgcacagc ggcgacatcg cctactggga cgaggacgag cacttcttca
     1441 tcgtggaccg gctgaagagc ctgatcaaat acaagggcta ccaggtagcc ccagccgaac
     1501 tggagagcat cctgctgcaa caccccaaca tcttcgacgc cggggtcgcc ggcctgcccg
     1561 acgacgatgc cggcgagctg cccgccgcag tcgtcgtgct ggaacacggt aaaaccatga
     1621 ccgagaagga gatcgtggac tatgtggcca gccaggttac aaccgccaag aagctgcgcg
     1681 gtggtgttgt gttcgtggac gaggtgccta aaggactgac cggcaagttg gacgcccgca
     1741 agatccgcga gattctcatt aaggccaaga agggcggcaa gatcgccgtg taataattct
     1801 agagtcgggg cggccggccg cttcgagcag acatgataag atacattgat gagtttggac
     1861 aaaccacaac tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt gatgctattg
     1921 ctttatttgt aaccattata agctgcaata aacaagttaa caacaacaat tgcattcatt
     1981 ttatgtttca ggttcagggg gaggtgtggg aggtttttta aagcaagtaa aacctctaca
     2041 aatgtggtaa aatcgataag gatccgtttg cgtattgggc gctcttccgc tgatctgcgc
     2101 agcaccatgg cctgaaataa cctctgaaag aggaacttgg ttagctacct tctgaggcgg
     2161 aaagaaccag ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc
     2221 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc
     2281 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt
     2341 cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc
     2401 ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctctg cctctgagct
     2461 attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctcgattc
     2521 ttctgacact agcgccacca tgaagaagcc cgaactcacc gctaccagcg ttgaaaaatt
     2581 tctcatcgag aagttcgaca gtgtgagcga cctgatgcag ttgtcggagg gcgaagagag
     2641 ccgagccttc agcttcgatg tcggcggacg cggctatgta ctgcgggtga atagctgcgc
     2701 tgatggcttc tacaaagacc gctacgtgta ccgccacttc gccagcgctg cactacccat
     2761 ccccgaagtg ttggacatcg gcgagttcag cgagagcctg acatactgca tcagtagacg
     2821 cgcccaaggc gttactctcc aagacctccc cgaaacagag ctgcctgctg tgttacagcc
     2881 tgtcgccgaa gctatggatg ctattgccgc cgccgacctc agtcaaacca gcggcttcgg
     2941 cccattcggg ccccaaggca tcggccagta cacaacctgg cgggatttca tttgcgccat
     3001 tgctgatccc catgtctacc actggcagac cgtgatggac gacaccgtgt ccgccagcgt
     3061 agctcaagcc ctggacgaac tgatgctgtg ggccgaagac tgtcccgagg tgcgccacct
     3121 cgtccatgcc gacttcggca gcaacaacgt cctgaccgac aacggccgca tcaccgccgt
     3181 aatcgactgg tccgaagcta tgttcgggga cagtcagtac gaggtggcca acatcttctt
     3241 ctggcggccc tggctggctt gcatggagca gcagactcgc tacttcgagc gccggcatcc
     3301 cgagctggcc ggcagccctc gtctgcgagc ctacatgctg cgcatcggcc tggatcagct
     3361 ctaccagagc ctcgtggacg gcaacttcga cgatgctgcc tgggctcaag gccgctgcga
     3421 tgccatcgtc cgcagcgggg ccggcaccgt cggtcgcaca caaatcgctc gccggagcgc
     3481 agccgtatgg accgacggct gcgtcgaggt gctggccgac agcggcaacc gccggcccag
     3541 tacacgaccg cgcgctaagg aggtaggtcg agtttaaact ctagaaccgg tcatggccgc
     3601 aataaaatat ctttattttc attacatctg tgtgttggtt ttttgtgtgt tcgaactaga
     3661 tgctgtcgac cgatgccctt gagagccttc aacccagtca gctccttccg gtgggcgcgg
     3721 ggcatgacta tcgtcgccgc acttatgact gtcttcttta tcatgcaact cgtaggacag
     3781 gtgccggcag cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
     3841 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
     3901 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
     3961 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
     4021 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
     4081 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
     4141 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
     4201 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
     4261 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
     4321 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
     4381 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct
     4441 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
     4501 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
     4561 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
     4621 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
     4681 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagcggccg
     4741 caaatgctaa accactgcag tggttaccag tgcttgatca gtgaggcacc gatctcagcg
     4801 atctgcctat ttcgttcgtc catagtggcc tgactccccg tcgtgtagat cactacgatt
     4861 cgtgagggct taccatcagg ccccagcgca gcaatgatgc cgcgagagcc gcgttcaccg
     4921 gcccccgatt tgtcagcaat gaaccagcca gcagggaggg ccgagcgaag aagtggtcct
     4981 gctactttgt ccgcctccat ccagtctatg agctgctgtc gtgatgctag agtaagaagt
     5041 tcgccagtga gtagtttccg aagagttgtg gccattgcta ctggcatcgt ggtatcacgc
     5101 tcgtcgttcg gtatggcttc gttcaactct ggttcccagc ggtcaagccg ggtcacatga
     5161 tcacccatat tatgaagaaa tgcagtcagc tccttagggc ctccgatcgt tgtcagaagt
     5221 aagttggccg cggtgttgtc gctcatggta atggcagcac tacacaattc tcttaccgtc
     5281 atgccatccg taagatgctt ttccgtgacc ggcgagtact caaccaagtc gttttgtgag
     5341 tagtgtatac ggcgaccaag ctgctcttgc ccggcgtcta tacgggacaa caccgcgcca
     5401 catagcagta ctttgaaagt gctcatcatc gggaatcgtt cttcggggcg gaaagactca
     5461 aggatcttgc cgctattgag atccagttcg atatagccca ctcttgcacc cagttgatct
     5521 tcagcatctt ttactttcac cagcgtttcg gggtgtgcaa aaacaggcaa gcaaaatgcc
     5581 gcaaagaagg gaatgagtgc gacacgaaaa tgttggatgc tcatactcgt cctttttcaa
     5641 tattattgaa gcatttatca gggttactag tacgtctctc aaggataagt aagtaatatt
     5701 aaggtacggg aggtattgga caggccgcaa taaaatatct ttattttcat tacatctgtg
     5761 tgttggtttt ttgtgtgaat cgatagtact aacatacgct ctccatcaaa acaaaacgaa
     5821 acaaaacaaa ctagcaaaat aggctgtccc cagtgcaagt gcaggtgcca gaacatttct
     5881 ct
//
