LOCUS       V005946                 4289 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V005946
VERSION     V005946
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 4289)
  AUTHORS   Koksharov M.
  TITLE     Design of short-lived firefly luciferase transcriptional reporters
            in mammalian cells: effects of N- or C-terminal modifications and
            interspecies differences on reporter half-life
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4289)
  AUTHORS   Koksharov M, Ugarova N.
  TITLE     Fast and simple in vivo photographic assessment of luciferase
            properties in bacterial cells to screen and compare mutant beetle
            luciferases: novel mutants illuminate luciferase color determinants
            and factors of protein stability in living cells
  JOURNAL   Unpublished
REFERENCE   3  (bases 1 to 4289)
  AUTHORS   Koksharov M.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-OCT-2016) Department of Chemical Enzymology, Faculty
            of Chemistry, Lomonosov Moscow State University, Leninskie gory,
            Bld. 1/11, Moscow, Moscow 119991, Russia
REFERENCE   4  (bases 1 to 4289)
  TITLE     Direct Submission
REFERENCE   5  (bases 1 to 4289)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 3; type: "Journal Article"; journalName: "Submitted
            (16-OCT-2016) Department of Chemical Enzymology, Faculty of
            Chemistry, Lomonosov Moscow State University, Leninskie gory, Bld.
            1/11, Moscow, Moscow 119991, Russia"
            SGRef: number: 4; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4289
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..70
                     /label="multiple cloning region"
                     /note="multiple cloning region"
     promoter        77..108
                     /label="minP"
                     /note="minimal TATA-box promoter with low basal activity"
     CDS             141..1778
                     /note="Luciferin 4-monooxygenase from Luciola mingrelica.
                     Accession#: Q26304"
                     /label="Luciferin 4-monooxygenase"
     polyA_signal    complement(1843..1964)
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2124..2142)
                     /label="RV4 reverse primer binding region"
                     /note="RV4 reverse primer binding region"
     rep_origin      complement(2383..2971)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(3174..4031)
                     /label="AmpR"
                     /note="beta-lactamase"
     polyA_signal    4136..4184
                     /label="poly(A) signal"
                     /note="synthetic polyadenylation signal"
     misc_feature    4198..4289
                     /label="pause site"
                     /note="RNA polymerase II transcriptional pause signal from
                     the human alpha-2 globin gene"
ORIGIN
        1 ggcctaactg gccggtacct gagctcgcta gcctcgagga tatcaagatc tggcctcggc
       61 ggccaagctt agacactaga gggtatataa tggaagctcg acttccagct tggcaatccg
      121 gtactgttgg taaagccacc atggaaatgg aaaaggagga gaatgttgta tatggccctc
      181 tgccattcta ccccattgaa gaaggatcag ctggaattca gttgcataag tacatgcatc
      241 aatatgccaa acttggagca attgcttttt cgaacgccct tactggagtt gacatttctt
      301 accaagaata ctttgatatt acatgtcgtt tagctgaggc catgaaaaac tttggtatga
      361 aaccggaaga acatattgct ttgtgcagtg aaaattgtga agaatttttc atccctgtac
      421 ttgctggtct ttacattggg gtagctgttg cacctactaa tgaaatttac acattgcgtg
      481 aacttaatca tagtttgggc atcgcacaac caactattgt attcagctcg agaaaaggct
      541 tacctaaagt tttagaagtg caaaaaacag ttacatgcat caaaaaaatt gttattttag
      601 atagtaaagt aaactttggg ggccacgatt gtatggaaac ttttattaag aaacatgtag
      661 aattaggttt tcaaccaagt agctttgtac ccattgatgt aaagaaccgt aaacaacacg
      721 ttgctttgct tatgaattct tctggctcta ctggtttacc taaaggtgta cgaattaccc
      781 acgaaggtgc agttacaaga ttctcacacg ctaaggatcc aatttacgga aaccaagttt
      841 cacctggtac tgctatttta actgtcgttc cgttccatca tggatttgga atgtttacca
      901 ctttaggata ctttgcttgt ggataccgtg ttgtaatgtt aacaaaattt gatgaagaac
      961 tatttttgag aactttgcaa gattataagt gtaccagtgt tattctggta ccaacgttat
     1021 ttgctattct caacaagagt gaattgatcg ataagttcga tttatctaat ctaactgaaa
     1081 ttgcttctgg tggagctcct ttggcaaaag aagttggcga agcagtcgct agaagattta
     1141 atctacccgg tgtccgtcag ggttacggat taacagaaac gacatctgca tttattatta
     1201 ccccagaagg tgatgataaa cctggagcat ctggaaaagt agtaccctta ttcaaagtaa
     1261 aagttattga tcttgacact aaaaaaactt tgggtgtcaa ccgacgagga gagatctgtg
     1321 taaaaggacc cagtcttatg ttaggctact cgaacaatcc ggaagcaaca agagaaacta
     1381 ttgatgaaga gggttggttg cacacaggag atattggata ttacgacgaa gacgaacatt
     1441 tcttcattgt cgaccgtttg aaatcattaa tcaaatacaa ggggtaccag gtaccacctg
     1501 ctgaattgga atccgttctt ttgcaacatc caaatatatt tgatgctggt gtggctggtg
     1561 tccccgatcc cgatgctggc gaacttccag gggctgtagt tgtaatggaa aaaggaaaaa
     1621 ctatgactga aaaggaaatt gtggattatg ttaatagtca agtagtgaac cacaaacgtc
     1681 tgcgtggtgg cgttcgtttt gtggatgaag tacctaaagg tctaactggt aaaattgatg
     1741 ctaaagtaat tagagaaatt cttaagaaac cacaatcggg cccactcgag gccgtgtaat
     1801 aattctagag tcggggcggc cggccgcttc gagcagacat gataagatac attgatgagt
     1861 ttggacaaac cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg
     1921 ctattgcttt atttgtaacc attataagct gcaataaaca agttaacaac aacaattgca
     1981 ttcattttat gtttcaggtt cagggggagg tgtgggaggt tttttaaagc aagtaaaacc
     2041 tctacaaatg tggtaaaatc gataaggatc cgtcgaccga tgcccttgag agccttcaac
     2101 ccagtcagct ccttccggtg ggcgcggggc atgactatcg tcgccgcact tatgactgtc
     2161 ttctttatca tgcaactcgt aggacaggtg ccggcagcgc tcttccgctt cctcgctcac
     2221 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
     2281 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
     2341 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
     2401 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     2461 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     2521 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
     2581 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     2641 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     2701 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
     2761 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
     2821 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
     2881 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
     2941 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
     3001 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
     3061 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
     3121 tgagtaaact tggtctgaca gcggccgcaa atgctaaacc actgcagtgg ttaccagtgc
     3181 ttgatcagtg aggcaccgat ctcagcgatc tgcctatttc gttcgtccat agtggcctga
     3241 ctccccgtcg tgtagatcac tacgattcgt gagggcttac catcaggccc cagcgcagca
     3301 atgatgccgc gagagccgcg ttcaccggcc cccgatttgt cagcaatgaa ccagccagca
     3361 gggagggccg agcgaagaag tggtcctgct actttgtccg cctccatcca gtctatgagc
     3421 tgctgtcgtg atgctagagt aagaagttcg ccagtgagta gtttccgaag agttgtggcc
     3481 attgctactg gcatcgtggt atcacgctcg tcgttcggta tggcttcgtt caactctggt
     3541 tcccagcggt caagccgggt cacatgatca cccatattat gaagaaatgc agtcagctcc
     3601 ttagggcctc cgatcgttgt cagaagtaag ttggccgcgg tgttgtcgct catggtaatg
     3661 gcagcactac acaattctct taccgtcatg ccatccgtaa gatgcttttc cgtgaccggc
     3721 gagtactcaa ccaagtcgtt ttgtgagtag tgtatacggc gaccaagctg ctcttgcccg
     3781 gcgtctatac gggacaacac cgcgccacat agcagtactt tgaaagtgct catcatcggg
     3841 aatcgttctt cggggcggaa agactcaagg atcttgccgc tattgagatc cagttcgata
     3901 tagcccactc ttgcacccag ttgatcttca gcatctttta ctttcaccag cgtttcgggg
     3961 tgtgcaaaaa caggcaagca aaatgccgca aagaagggaa tgagtgcgac acgaaaatgt
     4021 tggatgctca tactcgtcct ttttcaatat tattgaagca tttatcaggg ttactagtac
     4081 gtctctcaag gataagtaag taatattaag gtacgggagg tattggacag gccgcaataa
     4141 aatatcttta ttttcattac atctgtgtgt tggttttttg tgtgaatcga tagtactaac
     4201 atacgctctc catcaaaaca aaacgaaaca aaacaaacta gcaaaatagg ctgtccccag
     4261 tgcaagtgca ggtgccagaa catttctct
//
