LOCUS       pGL4.21[luc2P_Pu        5532 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Promoterless vector encoding destabilized luciferase for measuring 
            the activity of promoter and enhancer sequences.
ACCESSION   .
VERSION     .
KEYWORDS    pGL4.21[luc2P Puro]
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5532)
  AUTHORS   Promega
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 5532)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5532
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..70
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     CDS             100..1749
                     /label=luciferase
                     /note="firefly luciferase"
     CDS             1753..1872
                     /label=hPEST
                     /note="PEST degradation sequence from mouse ornithine 
                     decarboxylase"
     polyA_signal    complement(1924..2045)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        2239..2596
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             2627..3223
                     /label=PuroR
                     /note="puromycin N-acetyltransferase"
     polyA_signal    3251..3299
                     /label=poly(A) signal
                     /note="synthetic polyadenylation signal"
     rep_origin      complement(3626..4214)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4417..5274)
                     /label=AmpR
                     /note="beta-lactamase"
     polyA_signal    5379..5427
                     /label=synthetic polyadenylation signal
                     /note="synthetic polyadenylation signal"
     misc_feature    5441..5532
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from
                     the human alpha-2 globin gene"
ORIGIN
        1 ggcctaactg gccggtacct gagctcgcta gcctcgagga tatcaagatc tggcctcggc
       61 ggccaagctt ggcaatccgg tactgttggt aaagccacca tggaagatgc caaaaacatt
      121 aagaagggcc cagcgccatt ctacccactc gaagacggga ccgccggcga gcagctgcac
      181 aaagccatga agcgctacgc cctggtgccc ggcaccatcg cctttaccga cgcacatatc
      241 gaggtggaca ttacctacgc cgagtacttc gagatgagcg ttcggctggc agaagctatg
      301 aagcgctatg ggctgaatac aaaccatcgg atcgtggtgt gcagcgagaa tagcttgcag
      361 ttcttcatgc ccgtgttggg tgccctgttc atcggtgtgg ctgtggcccc agctaacgac
      421 atctacaacg agcgcgagct gctgaacagc atgggcatca gccagcccac cgtcgtattc
      481 gtgagcaaga aagggctgca aaagatcctc aacgtgcaaa agaagctacc gatcatacaa
      541 aagatcatca tcatggatag caagaccgac taccagggct tccaaagcat gtacaccttc
      601 gtgacttccc atttgccacc cggcttcaac gagtacgact tcgtgcccga gagcttcgac
      661 cgggacaaaa ccatcgccct gatcatgaac agtagtggca gtaccggatt gcccaagggc
      721 gtagccctac cgcaccgcac cgcttgtgtc cgattcagtc atgcccgcga ccccatcttc
      781 ggcaaccaga tcatccccga caccgctatc ctcagcgtgg tgccatttca ccacggcttc
      841 ggcatgttca ccacgctggg ctacttgatc tgcggctttc gggtcgtgct catgtaccgc
      901 ttcgaggagg agctattctt gcgcagcttg caagactata agattcaatc tgccctgctg
      961 gtgcccacac tatttagctt cttcgctaag agcactctca tcgacaagta cgacctaagc
     1021 aacttgcacg agatcgccag cggcggggcg ccgctcagca aggaggtagg tgaggccgtg
     1081 gccaaacgct tccacctacc aggcatccgc cagggctacg gcctgacaga aacaaccagc
     1141 gccattctga tcacccccga aggggacgac aagcctggcg cagtaggcaa ggtggtgccc
     1201 ttcttcgagg ctaaggtggt ggacttggac accggtaaga cactgggtgt gaaccagcgc
     1261 ggcgagctgt gcgtccgtgg ccccatgatc atgagcggct acgttaacaa ccccgaggct
     1321 acaaacgctc tcatcgacaa ggacggctgg ctgcacagcg gcgacatcgc ctactgggac
     1381 gaggacgagc acttcttcat cgtggaccgg ctgaagagcc tgatcaaata caagggctac
     1441 caggtagccc cagccgaact ggagagcatc ctgctgcaac accccaacat cttcgacgcc
     1501 ggggtcgccg gcctgcccga cgacgatgcc ggcgagctgc ccgccgcagt cgtcgtgctg
     1561 gaacacggta aaaccatgac cgagaaggag atcgtggact atgtggccag ccaggttaca
     1621 accgccaaga agctgcgcgg tggtgttgtg ttcgtggacg aggtgcctaa aggactgacc
     1681 ggcaagttgg acgcccgcaa gatccgcgag attctcatta aggccaagaa gggcggcaag
     1741 atcgccgtga attctcacgg cttccctccc gaggtggagg agcaggccgc cggcaccctg
     1801 cccatgagct gcgcccagga gagcggcatg gatagacacc ctgctgcttg cgccagcgcc
     1861 aggatcaacg tctaaggccg cgactctaga gtcggggcgg ccggccgctt cgagcagaca
     1921 tgataagata cattgatgag tttggacaaa ccacaactag aatgcagtga aaaaaatgct
     1981 ttatttgtga aatttgtgat gctattgctt tatttgtaac cattataagc tgcaataaac
     2041 aagttaacaa caacaattgc attcatttta tgtttcaggt tcagggggag gtgtgggagg
     2101 ttttttaaag caagtaaaac ctctacaaat gtggtaaaat cgataaggat ccgtttgcgt
     2161 attgggcgct cttccgctga tctgcgcagc accatggcct gaaataacct ctgaaagagg
     2221 aacttggtta gctaccttct gaggcggaaa gaaccagctg tggaatgtgt gtcagttagg
     2281 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta
     2341 gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat
     2401 gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac
     2461 tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga
     2521 ggccgaggcc gcctctgcct ctgagctatt ccagaagtag tgaggaggct tttttggagg
     2581 cctaggcttt tgcaaaaagc tcgattcttc tgacactagc gccaccatga ccgagtacaa
     2641 gcctaccgtg cgcctggcca ctcgcgatga tgtgccccgc gccgtccgca ctctggccgc
     2701 cgctttcgcc gactaccccg ctacccggca caccgtggac cccgaccggc acatcgagcg
     2761 tgtgacagag ttgcaggagc tgttcctgac ccgcgtcggg ctggacatcg gcaaggtgtg
     2821 ggtagccgac gacggcgcgg ccgtggccgt gtggactacc cccgagagcg ttgaggccgg
     2881 cgccgtgttc gccgagatcg gcccccgaat ggccgagctg agcggcagcc gcctggccgc
     2941 ccagcagcaa atggagggcc tgcttgcccc ccatcgtccc aaggagcctg cctggtttct
     3001 ggccactgta ggagtgagcc ccgaccacca gggcaagggc ttgggcagcg ccgtcgtgtt
     3061 gcccggcgta gaggccgccg aacgcgccgg tgtgcccgcc tttctcgaaa caagcgcacc
     3121 aagaaacctt ccattctacg agcgcctggg cttcaccgtg accgccgatg tcgaggtgcc
     3181 cgagggacct aggacctggt gtatgacacg aaaacctggc gcctaatgat ctagaaccgg
     3241 tcatggccgc aataaaatat ctttattttc attacatctg tgtgttggtt ttttgtgtgt
     3301 tcgaactaga tgctgtcgac cgatgccctt gagagccttc aacccagtca gctccttccg
     3361 gtgggcgcgg ggcatgacta tcgtcgccgc acttatgact gtcttcttta tcatgcaact
     3421 cgtaggacag gtgccggcag cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
     3481 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
     3541 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
     3601 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
     3661 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
     3721 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
     3781 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
     3841 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
     3901 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
     3961 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
     4021 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta
     4081 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
     4141 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
     4201 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
     4261 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
     4321 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
     4381 acagcggccg caaatgctaa accactgcag tggttaccag tgcttgatca gtgaggcacc
     4441 gatctcagcg atctgcctat ttcgttcgtc catagtggcc tgactccccg tcgtgtagat
     4501 cactacgatt cgtgagggct taccatcagg ccccagcgca gcaatgatgc cgcgagagcc
     4561 gcgttcaccg gcccccgatt tgtcagcaat gaaccagcca gcagggaggg ccgagcgaag
     4621 aagtggtcct gctactttgt ccgcctccat ccagtctatg agctgctgtc gtgatgctag
     4681 agtaagaagt tcgccagtga gtagtttccg aagagttgtg gccattgcta ctggcatcgt
     4741 ggtatcacgc tcgtcgttcg gtatggcttc gttcaactct ggttcccagc ggtcaagccg
     4801 ggtcacatga tcacccatat tatgaagaaa tgcagtcagc tccttagggc ctccgatcgt
     4861 tgtcagaagt aagttggccg cggtgttgtc gctcatggta atggcagcac tacacaattc
     4921 tcttaccgtc atgccatccg taagatgctt ttccgtgacc ggcgagtact caaccaagtc
     4981 gttttgtgag tagtgtatac ggcgaccaag ctgctcttgc ccggcgtcta tacgggacaa
     5041 caccgcgcca catagcagta ctttgaaagt gctcatcatc gggaatcgtt cttcggggcg
     5101 gaaagactca aggatcttgc cgctattgag atccagttcg atatagccca ctcttgcacc
     5161 cagttgatct tcagcatctt ttactttcac cagcgtttcg gggtgtgcaa aaacaggcaa
     5221 gcaaaatgcc gcaaagaagg gaatgagtgc gacacgaaaa tgttggatgc tcatactcgt
     5281 cctttttcaa tattattgaa gcatttatca gggttactag tacgtctctc aaggataagt
     5341 aagtaatatt aaggtacggg aggtattgga caggccgcaa taaaatatct ttattttcat
     5401 tacatctgtg tgttggtttt ttgtgtgaat cgatagtact aacatacgct ctccatcaaa
     5461 acaaaacgaa acaaaacaaa ctagcaaaat aggctgtccc cagtgcaagt gcaggtgcca
     5521 gaacatttct ct
//
