LOCUS       Exported                5404 bp DNA     circular SYN 10-SEP-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5404)
  AUTHORS   vfsd
  TITLE     Direct Submission
  JOURNAL   Exported Sep 10, 2025 from SnapGene 6.0.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5404
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    70..118
                     /note="synthetic polyadenylation signal"
     misc_feature    132..223
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     CDS             323..1975
                     /codon_start=1
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="synthetic luc2 version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     polyA_signal    2019..2140
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        2334..2691
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      2542..2677
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2722..3321
                     /codon_start=1
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     polyA_signal    3346..3394
                     /note="synthetic polyadenylation signal"
     rep_origin      complement(3721..4309)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4509..5369)
                     /codon_start=1
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 agggttacta gtacgtctct caaggataag taagtaatat taaggtacgg gaggtattgg
       61 acaggccgca ataaaatatc tttattttca ttacatctgt gtgttggttt tttgtgtgaa
      121 tcgatagtac taacatacgc tctccatcaa aacaaaacga aacaaaacaa actagcaaaa
      181 taggctgtcc ccagtgcaag tgcaggtgcc agaacatttc tctggcctaa ctggccggta
      241 cctgagctcg ctagcctcga ggatatcaag atctggcctc ggcggccaag cttggcaatc
      301 cggtactgtt ggtaaagcca ccatggaaga tgccaaaaac attaagaagg gcccagcgcc
      361 attctaccca ctcgaagacg ggaccgccgg cgagcagctg cacaaagcca tgaagcgcta
      421 cgccctggtg cccggcacca tcgcctttac cgacgcacat atcgaggtgg acattaccta
      481 cgccgagtac ttcgagatga gcgttcggct ggcagaagct atgaagcgct atgggctgaa
      541 tacaaaccat cggatcgtgg tgtgcagcga gaatagcttg cagttcttca tgcccgtgtt
      601 gggtgccctg ttcatcggtg tggctgtggc cccagctaac gacatctaca acgagcgcga
      661 gctgctgaac agcatgggca tcagccagcc caccgtcgta ttcgtgagca agaaagggct
      721 gcaaaagatc ctcaacgtgc aaaagaagct accgatcata caaaagatca tcatcatgga
      781 tagcaagacc gactaccagg gcttccaaag catgtacacc ttcgtgactt cccatttgcc
      841 acccggcttc aacgagtacg acttcgtgcc cgagagcttc gaccgggaca aaaccatcgc
      901 cctgatcatg aacagtagtg gcagtaccgg attgcccaag ggcgtagccc taccgcaccg
      961 caccgcttgt gtccgattca gtcatgcccg cgaccccatc ttcggcaacc agatcatccc
     1021 cgacaccgct atcctcagcg tggtgccatt tcaccacggc ttcggcatgt tcaccacgct
     1081 gggctacttg atctgcggct ttcgggtcgt gctcatgtac cgcttcgagg aggagctatt
     1141 cttgcgcagc ttgcaagact ataagattca atctgccctg ctggtgccca cactatttag
     1201 cttcttcgct aagagcactc tcatcgacaa gtacgaccta agcaacttgc acgagatcgc
     1261 cagcggcggg gcgccgctca gcaaggaggt aggtgaggcc gtggccaaac gcttccacct
     1321 accaggcatc cgccagggct acggcctgac agaaacaacc agcgccattc tgatcacccc
     1381 cgaaggggac gacaagcctg gcgcagtagg caaggtggtg cccttcttcg aggctaaggt
     1441 ggtggacttg gacaccggta agacactggg tgtgaaccag cgcggcgagc tgtgcgtccg
     1501 tggccccatg atcatgagcg gctacgttaa caaccccgag gctacaaacg ctctcatcga
     1561 caaggacggc tggctgcaca gcggcgacat cgcctactgg gacgaggacg agcacttctt
     1621 catcgtggac cggctgaaga gcctgatcaa atacaagggc taccaggtag ccccagccga
     1681 actggagagc atcctgctgc aacaccccaa catcttcgac gccggggtcg ccggcctgcc
     1741 cgacgacgat gccggcgagc tgcccgccgc agtcgtcgtg ctggaacacg gtaaaaccat
     1801 gaccgagaag gagatcgtgg actatgtggc cagccaggtt acaaccgcca agaagctgcg
     1861 cggtggtgtt gtgttcgtgg acgaggtgcc taaaggactg accggcaagt tggacgcccg
     1921 caagatccgc gagattctca ttaaggccaa gaagggcggc aagatcgccg tgtaataatt
     1981 ctagagtcgg ggcggccggc cgcttcgagc agacatgata agatacattg atgagtttgg
     2041 acaaaccaca actagaatgc agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat
     2101 tgctttattt gtaaccatta taagctgcaa taaacaagtt aacaacaaca attgcattca
     2161 ttttatgttt caggttcagg gggaggtgtg ggaggttttt taaagcaagt aaaacctcta
     2221 caaatgtggt aaaatcgata aggatccgtt tgcgtattgg gcgctcttcc gctgatctgc
     2281 gcagcaccat ggcctgaaat aacctctgaa agaggaactt ggttagctac cttctgaggc
     2341 ggaaagaacc agctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca
     2401 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc
     2461 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata
     2521 gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg
     2581 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc tgcctctgag
     2641 ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagctcgat
     2701 tcttctgaca ctagcgccac catgaccgag tacaagccta ccgtgcgcct ggccactcgc
     2761 gatgatgtgc cccgcgccgt ccgcactctg gccgccgctt tcgccgacta ccccgctacc
     2821 cggcacaccg tggaccccga ccggcacatc gagcgtgtga cagagttgca ggagctgttc
     2881 ctgacccgcg tcgggctgga catcggcaag gtgtgggtag ccgacgacgg cgcggccgtg
     2941 gccgtgtgga ctacccccga gagcgttgag gccggcgccg tgttcgccga gatcggcccc
     3001 cgaatggccg agctgagcgg cagccgcctg gccgcccagc agcaaatgga gggcctgctt
     3061 gccccccatc gtcccaagga gcctgcctgg tttctggcca ctgtaggagt gagccccgac
     3121 caccagggca agggcttggg cagcgccgtc gtgttgcccg gcgtagaggc cgccgaacgc
     3181 gccggtgtgc ccgcctttct cgaaacaagc gcaccaagaa accttccatt ctacgagcgc
     3241 ctgggcttca ccgtgaccgc cgatgtcgag gtgcccgagg gacctaggac ctggtgtatg
     3301 acacgaaaac ctggcgccta atgatctaga accggtcatg gccgcaataa aatatcttta
     3361 ttttcattac atctgtgtgt tggttttttg tgtgttcgaa ctagatgctg tcgaccgatg
     3421 cccttgagag ccttcaaccc agtcagctcc ttccggtggg cgcggggcat gactatcgtc
     3481 gccgcactta tgactgtctt ctttatcatg caactcgtag gacaggtgcc ggcagcgctc
     3541 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
     3601 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
     3661 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     3721 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     3781 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     3841 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     3901 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     3961 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     4021 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     4081 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     4141 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
     4201 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
     4261 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     4321 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
     4381 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
     4441 atcaatctaa agtatatatg agtaaacttg gtctgacagc ggccgcaaat gctaaaccac
     4501 tgcagtggtt accagtgctt gatcagtgag gcaccgatct cagcgatctg cctatttcgt
     4561 tcgtccatag tggcctgact ccccgtcgtg tagatcacta cgattcgtga gggcttacca
     4621 tcaggcccca gcgcagcaat gatgccgcga gagccgcgtt caccggcccc cgatttgtca
     4681 gcaatgaacc agccagcagg gagggccgag cgaagaagtg gtcctgctac tttgtccgcc
     4741 tccatccagt ctatgagctg ctgtcgtgat gctagagtaa gaagttcgcc agtgagtagt
     4801 ttccgaagag ttgtggccat tgctactggc atcgtggtat cacgctcgtc gttcggtatg
     4861 gcttcgttca actctggttc ccagcggtca agccgggtca catgatcacc catattatga
     4921 agaaatgcag tcagctcctt agggcctccg atcgttgtca gaagtaagtt ggccgcggtg
     4981 ttgtcgctca tggtaatggc agcactacac aattctctta ccgtcatgcc atccgtaaga
     5041 tgcttttccg tgaccggcga gtactcaacc aagtcgtttt gtgagtagtg tatacggcga
     5101 ccaagctgct cttgcccggc gtctatacgg gacaacaccg cgccacatag cagtactttg
     5161 aaagtgctca tcatcgggaa tcgttcttcg gggcggaaag actcaaggat cttgccgcta
     5221 ttgagatcca gttcgatata gcccactctt gcacccagtt gatcttcagc atcttttact
     5281 ttcaccagcg tttcggggtg tgcaaaaaca ggcaagcaaa atgccgcaaa gaagggaatg
     5341 agtgcgacac gaaaatgttg gatgctcata ctcgtccttt ttcaatatta ttgaagcatt
     5401 tatc
//
