LOCUS       62056_11690        5849 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5849)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..5849
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             108..1757
                     /codon_start=1
                     /label=luciferase
                     /note="firefly luciferase"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     polyA_signal    complement(1804..1925)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        2119..2476
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             2507..3541
                     /codon_start=1
                     /label=HygR
                     /note="hygromycin B phosphotransferase"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     EVGRV"
     polyA_signal    3568..3616
                     /label=poly(A) signal
                     /note="synthetic polyadenylation signal"
     rep_origin      complement(3943..4531)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4734..5591)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     misc_feature    5758..5849
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from
                     the human alpha-2 globin gene"
ORIGIN
        1 ggcctaactg gccggtacct gagctcgcta gcctcgagga tatctgactc atgactcatg
       61 actcatgact caaagcttgg caatccggta ctgttggtaa agccaccatg gaagatgcca
      121 aaaacattaa gaagggccca gcgccattct acccactcga agacgggacc gccggcgagc
      181 agctgcacaa agccatgaag cgctacgccc tggtgcccgg caccatcgcc tttaccgacg
      241 cacatatcga ggtggacatt acctacgccg agtacttcga gatgagcgtt cggctggcag
      301 aagctatgaa gcgctatggg ctgaatacaa accatcggat cgtggtgtgc agcgagaata
      361 gcttgcagtt cttcatgccc gtgttgggtg ccctgttcat cggtgtggct gtggccccag
      421 ctaacgacat ctacaacgag cgcgagctgc tgaacagcat gggcatcagc cagcccaccg
      481 tcgtattcgt gagcaagaaa gggctgcaaa agatcctcaa cgtgcaaaag aagctaccga
      541 tcatacaaaa gatcatcatc atggatagca agaccgacta ccagggcttc caaagcatgt
      601 acaccttcgt gacttcccat ttgccacccg gcttcaacga gtacgacttc gtgcccgaga
      661 gcttcgaccg ggacaaaacc atcgccctga tcatgaacag tagtggcagt accggattgc
      721 ccaagggcgt agccctaccg caccgcaccg cttgtgtccg attcagtcat gcccgcgacc
      781 ccatcttcgg caaccagatc atccccgaca ccgctatcct cagcgtggtg ccatttcacc
      841 acggcttcgg catgttcacc acgctgggct acttgatctg cggctttcgg gtcgtgctca
      901 tgtaccgctt cgaggaggag ctattcttgc gcagcttgca agactataag attcaatctg
      961 ccctgctggt gcccacacta tttagcttct tcgctaagag cactctcatc gacaagtacg
     1021 acctaagcaa cttgcacgag atcgccagcg gcggggcgcc gctcagcaag gaggtaggtg
     1081 aggccgtggc caaacgcttc cacctaccag gcatccgcca gggctacggc ctgacagaaa
     1141 caaccagcgc cattctgatc acccccgaag gggacgacaa gcctggcgca gtaggcaagg
     1201 tggtgccctt cttcgaggct aaggtggtgg acttggacac cggtaagaca ctgggtgtga
     1261 accagcgcgg cgagctgtgc gtccgtggcc ccatgatcat gagcggctac gttaacaacc
     1321 ccgaggctac aaacgctctc atcgacaagg acggctggct gcacagcggc gacatcgcct
     1381 actgggacga ggacgagcac ttcttcatcg tggaccggct gaagagcctg atcaaataca
     1441 agggctacca ggtagcccca gccgaactgg agagcatcct gctgcaacac cccaacatct
     1501 tcgacgccgg ggtcgccggc ctgcccgacg acgatgccgg cgagctgccc gccgcagtcg
     1561 tcgtgctgga acacggtaaa accatgaccg agaaggagat cgtggactat gtggccagcc
     1621 aggttacaac cgccaagaag ctgcgcggtg gtgttgtgtt cgtggacgag gtgcctaaag
     1681 gactgaccgg caagttggac gcccgcaaga tccgcgagat tctcattaag gccaagaagg
     1741 gcggcaagat cgccgtgtaa taattctaga gtcggggcgg ccggccgctt cgagcagaca
     1801 tgataagata cattgatgag tttggacaaa ccacaactag aatgcagtga aaaaaatgct
     1861 ttatttgtga aatttgtgat gctattgctt tatttgtaac cattataagc tgcaataaac
     1921 aagttaacaa caacaattgc attcatttta tgtttcaggt tcagggggag gtgtgggagg
     1981 ttttttaaag caagtaaaac ctctacaaat gtggtaaaat cgataaggat ccgtttgcgt
     2041 attgggcgct cttccgctga tctgcgcagc accatggcct gaaataacct ctgaaagagg
     2101 aacttggtta gctaccttct gaggcggaaa gaaccagctg tggaatgtgt gtcagttagg
     2161 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta
     2221 gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat
     2281 gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac
     2341 tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga
     2401 ggccgaggcc gcctctgcct ctgagctatt ccagaagtag tgaggaggct tttttggagg
     2461 cctaggcttt tgcaaaaagc tcgattcttc tgacactagc gccaccatga agaagcccga
     2521 actcaccgct accagcgttg aaaaatttct catcgagaag ttcgacagtg tgagcgacct
     2581 gatgcagttg tcggagggcg aagagagccg agccttcagc ttcgatgtcg gcggacgcgg
     2641 ctatgtactg cgggtgaata gctgcgctga tggcttctac aaagaccgct acgtgtaccg
     2701 ccacttcgcc agcgctgcac tacccatccc cgaagtgttg gacatcggcg agttcagcga
     2761 gagcctgaca tactgcatca gtagacgcgc ccaaggcgtt actctccaag acctccccga
     2821 aacagagctg cctgctgtgt tacagcctgt cgccgaagct atggatgcta ttgccgccgc
     2881 cgacctcagt caaaccagcg gcttcggccc attcgggccc caaggcatcg gccagtacac
     2941 aacctggcgg gatttcattt gcgccattgc tgatccccat gtctaccact ggcagaccgt
     3001 gatggacgac accgtgtccg ccagcgtagc tcaagccctg gacgaactga tgctgtgggc
     3061 cgaagactgt cccgaggtgc gccacctcgt ccatgccgac ttcggcagca acaacgtcct
     3121 gaccgacaac ggccgcatca ccgccgtaat cgactggtcc gaagctatgt tcggggacag
     3181 tcagtacgag gtggccaaca tcttcttctg gcggccctgg ctggcttgca tggagcagca
     3241 gactcgctac ttcgagcgcc ggcatcccga gctggccggc agccctcgtc tgcgagccta
     3301 catgctgcgc atcggcctgg atcagctcta ccagagcctc gtggacggca acttcgacga
     3361 tgctgcctgg gctcaaggcc gctgcgatgc catcgtccgc agcggggccg gcaccgtcgg
     3421 tcgcacacaa atcgctcgcc ggagcgcagc cgtatggacc gacggctgcg tcgaggtgct
     3481 ggccgacagc ggcaaccgcc ggcccagtac acgaccgcgc gctaaggagg taggtcgagt
     3541 ttaaactcta gaaccggtca tggccgcaat aaaatatctt tattttcatt acatctgtgt
     3601 gttggttttt tgtgtgttcg aactagatgc tgtcgaccga tgcccttgag agccttcaac
     3661 ccagtcagct ccttccggtg ggcgcggggc atgactatcg tcgccgcact tatgactgtc
     3721 ttctttatca tgcaactcgt aggacaggtg ccggcagcgc tcttccgctt cctcgctcac
     3781 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
     3841 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
     3901 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
     3961 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     4021 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     4081 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
     4141 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     4201 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     4261 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
     4321 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
     4381 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
     4441 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
     4501 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
     4561 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
     4621 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
     4681 tgagtaaact tggtctgaca gcggccgcaa atgctaaacc actgcagtgg ttaccagtgc
     4741 ttgatcagtg aggcaccgat ctcagcgatc tgcctatttc gttcgtccat agtggcctga
     4801 ctccccgtcg tgtagatcac tacgattcgt gagggcttac catcaggccc cagcgcagca
     4861 atgatgccgc gagagccgcg ttcaccggcc cccgatttgt cagcaatgaa ccagccagca
     4921 gggagggccg agcgaagaag tggtcctgct actttgtccg cctccatcca gtctatgagc
     4981 tgctgtcgtg atgctagagt aagaagttcg ccagtgagta gtttccgaag agttgtggcc
     5041 attgctactg gcatcgtggt atcacgctcg tcgttcggta tggcttcgtt caactctggt
     5101 tcccagcggt caagccgggt cacatgatca cccatattat gaagaaatgc agtcagctcc
     5161 ttagggcctc cgatcgttgt cagaagtaag ttggccgcgg tgttgtcgct catggtaatg
     5221 gcagcactac acaattctct taccgtcatg ccatccgtaa gatgcttttc cgtgaccggc
     5281 gagtactcaa ccaagtcgtt ttgtgagtag tgtatacggc gaccaagctg ctcttgcccg
     5341 gcgtctatac gggacaacac cgcgccacat agcagtactt tgaaagtgct catcatcggg
     5401 aatcgttctt cggggcggaa agactcaagg atcttgccgc tattgagatc cagttcgata
     5461 tagcccactc ttgcacccag ttgatcttca gcatctttta ctttcaccag cgtttcgggg
     5521 tgtgcaaaaa caggcaagca aaatgccgca aagaagggaa tgagtgcgac acgaaaatgt
     5581 tggatgctca tactcgtcct ttttcaatat tattgaagca tttatcaggg ttactagtac
     5641 gtctctcaag gataagtaag taatattaag gtacgggagg tattggacag gccgcaataa
     5701 aatatcttta ttttcattac atctgtgtgt tggttttttg tgtgaatcga tagtactaac
     5761 atacgctctc catcaaaaca aaacgaaaca aaacaaacta gcaaaatagg ctgtccccag
     5821 tgcaagtgca ggtgccagaa catttctct
//
