LOCUS       pGL4.11[luc2P].        4370 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Promoterless vector encoding destabilized luciferase for measuring 
            the activity of promoter and enhancer sequences.
ACCESSION   .
VERSION     .
KEYWORDS    pGL4.11[luc2P]
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4370)
  AUTHORS   Promega
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 4370)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4370
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..70
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     CDS             100..1749
                     /label=luciferase
                     /note="firefly luciferase"
     CDS             1753..1872
                     /label=hPEST
                     /note="PEST degradation sequence from mouse ornithine 
                     decarboxylase"
     polyA_signal    complement(1924..2045)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(2464..3052)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3255..4112)
                     /label=AmpR
                     /note="beta-lactamase"
     polyA_signal    4217..4265
                     /label=poly(A) signal
                     /note="synthetic polyadenylation signal"
     misc_feature    4279..4370
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from
                     the human alpha-2 globin gene"
ORIGIN
        1 ggcctaactg gccggtacct gagctcgcta gcctcgagga tatcaagatc tggcctcggc
       61 ggccaagctt ggcaatccgg tactgttggt aaagccacca tggaagatgc caaaaacatt
      121 aagaagggcc cagcgccatt ctacccactc gaagacggga ccgccggcga gcagctgcac
      181 aaagccatga agcgctacgc cctggtgccc ggcaccatcg cctttaccga cgcacatatc
      241 gaggtggaca ttacctacgc cgagtacttc gagatgagcg ttcggctggc agaagctatg
      301 aagcgctatg ggctgaatac aaaccatcgg atcgtggtgt gcagcgagaa tagcttgcag
      361 ttcttcatgc ccgtgttggg tgccctgttc atcggtgtgg ctgtggcccc agctaacgac
      421 atctacaacg agcgcgagct gctgaacagc atgggcatca gccagcccac cgtcgtattc
      481 gtgagcaaga aagggctgca aaagatcctc aacgtgcaaa agaagctacc gatcatacaa
      541 aagatcatca tcatggatag caagaccgac taccagggct tccaaagcat gtacaccttc
      601 gtgacttccc atttgccacc cggcttcaac gagtacgact tcgtgcccga gagcttcgac
      661 cgggacaaaa ccatcgccct gatcatgaac agtagtggca gtaccggatt gcccaagggc
      721 gtagccctac cgcaccgcac cgcttgtgtc cgattcagtc atgcccgcga ccccatcttc
      781 ggcaaccaga tcatccccga caccgctatc ctcagcgtgg tgccatttca ccacggcttc
      841 ggcatgttca ccacgctggg ctacttgatc tgcggctttc gggtcgtgct catgtaccgc
      901 ttcgaggagg agctattctt gcgcagcttg caagactata agattcaatc tgccctgctg
      961 gtgcccacac tatttagctt cttcgctaag agcactctca tcgacaagta cgacctaagc
     1021 aacttgcacg agatcgccag cggcggggcg ccgctcagca aggaggtagg tgaggccgtg
     1081 gccaaacgct tccacctacc aggcatccgc cagggctacg gcctgacaga aacaaccagc
     1141 gccattctga tcacccccga aggggacgac aagcctggcg cagtaggcaa ggtggtgccc
     1201 ttcttcgagg ctaaggtggt ggacttggac accggtaaga cactgggtgt gaaccagcgc
     1261 ggcgagctgt gcgtccgtgg ccccatgatc atgagcggct acgttaacaa ccccgaggct
     1321 acaaacgctc tcatcgacaa ggacggctgg ctgcacagcg gcgacatcgc ctactgggac
     1381 gaggacgagc acttcttcat cgtggaccgg ctgaagagcc tgatcaaata caagggctac
     1441 caggtagccc cagccgaact ggagagcatc ctgctgcaac accccaacat cttcgacgcc
     1501 ggggtcgccg gcctgcccga cgacgatgcc ggcgagctgc ccgccgcagt cgtcgtgctg
     1561 gaacacggta aaaccatgac cgagaaggag atcgtggact atgtggccag ccaggttaca
     1621 accgccaaga agctgcgcgg tggtgttgtg ttcgtggacg aggtgcctaa aggactgacc
     1681 ggcaagttgg acgcccgcaa gatccgcgag attctcatta aggccaagaa gggcggcaag
     1741 atcgccgtga attctcacgg cttccctccc gaggtggagg agcaggccgc cggcaccctg
     1801 cccatgagct gcgcccagga gagcggcatg gatagacacc ctgctgcttg cgccagcgcc
     1861 aggatcaacg tctaaggccg cgactctaga gtcggggcgg ccggccgctt cgagcagaca
     1921 tgataagata cattgatgag tttggacaaa ccacaactag aatgcagtga aaaaaatgct
     1981 ttatttgtga aatttgtgat gctattgctt tatttgtaac cattataagc tgcaataaac
     2041 aagttaacaa caacaattgc attcatttta tgtttcaggt tcagggggag gtgtgggagg
     2101 ttttttaaag caagtaaaac ctctacaaat gtggtaaaat cgataaggat ccgtcgaccg
     2161 atgcccttga gagccttcaa cccagtcagc tccttccggt gggcgcgggg catgactatc
     2221 gtcgccgcac ttatgactgt cttctttatc atgcaactcg taggacaggt gccggcagcg
     2281 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
     2341 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
     2401 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
     2461 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     2521 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     2581 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     2641 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     2701 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     2761 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     2821 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     2881 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt
     2941 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     3001 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
     3061 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
     3121 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
     3181 taaatcaatc taaagtatat atgagtaaac ttggtctgac agcggccgca aatgctaaac
     3241 cactgcagtg gttaccagtg cttgatcagt gaggcaccga tctcagcgat ctgcctattt
     3301 cgttcgtcca tagtggcctg actccccgtc gtgtagatca ctacgattcg tgagggctta
     3361 ccatcaggcc ccagcgcagc aatgatgccg cgagagccgc gttcaccggc ccccgatttg
     3421 tcagcaatga accagccagc agggagggcc gagcgaagaa gtggtcctgc tactttgtcc
     3481 gcctccatcc agtctatgag ctgctgtcgt gatgctagag taagaagttc gccagtgagt
     3541 agtttccgaa gagttgtggc cattgctact ggcatcgtgg tatcacgctc gtcgttcggt
     3601 atggcttcgt tcaactctgg ttcccagcgg tcaagccggg tcacatgatc acccatatta
     3661 tgaagaaatg cagtcagctc cttagggcct ccgatcgttg tcagaagtaa gttggccgcg
     3721 gtgttgtcgc tcatggtaat ggcagcacta cacaattctc ttaccgtcat gccatccgta
     3781 agatgctttt ccgtgaccgg cgagtactca accaagtcgt tttgtgagta gtgtatacgg
     3841 cgaccaagct gctcttgccc ggcgtctata cgggacaaca ccgcgccaca tagcagtact
     3901 ttgaaagtgc tcatcatcgg gaatcgttct tcggggcgga aagactcaag gatcttgccg
     3961 ctattgagat ccagttcgat atagcccact cttgcaccca gttgatcttc agcatctttt
     4021 actttcacca gcgtttcggg gtgtgcaaaa acaggcaagc aaaatgccgc aaagaaggga
     4081 atgagtgcga cacgaaaatg ttggatgctc atactcgtcc tttttcaata ttattgaagc
     4141 atttatcagg gttactagta cgtctctcaa ggataagtaa gtaatattaa ggtacgggag
     4201 gtattggaca ggccgcaata aaatatcttt attttcatta catctgtgtg ttggtttttt
     4261 gtgtgaatcg atagtactaa catacgctct ccatcaaaac aaaacgaaac aaaacaaact
     4321 agcaaaatag gctgtcccca gtgcaagtgc aggtgccaga acatttctct
//
