LOCUS       Exported                4242 bp DNA     circular SYN 11-SEP-2025
DEFINITION  Promoterless vector for measuring the activity of promoter and 
            enhancer sequences with a luciferase assay.
ACCESSION   .
VERSION     .
KEYWORDS    pGL4.10[luc2]
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4242)
  AUTHORS   Promega
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 4242)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 4242)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Sep 11, 2025 from SnapGene 6.0.2
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4242
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     source          join(214..4242,1..213)
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    60..108
                     /label=poly(A) signal
                     /note="synthetic polyadenylation signal"
     misc_feature    122..213
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from
                     the human alpha-2 globin gene"
     misc_feature    214..283
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     CDS             313..1962
                     /label=luciferase
                     /note="firefly luciferase"
     polyA_signal    complement(2009..2130)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(2549..3137)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3340..4197)
                     /label=AmpR
                     /note="beta-lactamase"
ORIGIN
        1 gtacgtctct caaggataag taagtaatat taaggtacgg gaggtattgg acaggccgca
       61 ataaaatatc tttattttca ttacatctgt gtgttggttt tttgtgtgaa tcgatagtac
      121 taacatacgc tctccatcaa aacaaaacga aacaaaacaa actagcaaaa taggctgtcc
      181 ccagtgcaag tgcaggtgcc agaacatttc tctggcctaa ctggccggta cctgagctcg
      241 ctagcctcga ggatatcaag atctggcctc ggcggccaag cttggcaatc cggtactgtt
      301 ggtaaagcca ccatggaaga tgccaaaaac attaagaagg gcccagcgcc attctaccca
      361 ctcgaagacg ggaccgccgg cgagcagctg cacaaagcca tgaagcgcta cgccctggtg
      421 cccggcacca tcgcctttac cgacgcacat atcgaggtgg acattaccta cgccgagtac
      481 ttcgagatga gcgttcggct ggcagaagct atgaagcgct atgggctgaa tacaaaccat
      541 cggatcgtgg tgtgcagcga gaatagcttg cagttcttca tgcccgtgtt gggtgccctg
      601 ttcatcggtg tggctgtggc cccagctaac gacatctaca acgagcgcga gctgctgaac
      661 agcatgggca tcagccagcc caccgtcgta ttcgtgagca agaaagggct gcaaaagatc
      721 ctcaacgtgc aaaagaagct accgatcata caaaagatca tcatcatgga tagcaagacc
      781 gactaccagg gcttccaaag catgtacacc ttcgtgactt cccatttgcc acccggcttc
      841 aacgagtacg acttcgtgcc cgagagcttc gaccgggaca aaaccatcgc cctgatcatg
      901 aacagtagtg gcagtaccgg attgcccaag ggcgtagccc taccgcaccg caccgcttgt
      961 gtccgattca gtcatgcccg cgaccccatc ttcggcaacc agatcatccc cgacaccgct
     1021 atcctcagcg tggtgccatt tcaccacggc ttcggcatgt tcaccacgct gggctacttg
     1081 atctgcggct ttcgggtcgt gctcatgtac cgcttcgagg aggagctatt cttgcgcagc
     1141 ttgcaagact ataagattca atctgccctg ctggtgccca cactatttag cttcttcgct
     1201 aagagcactc tcatcgacaa gtacgaccta agcaacttgc acgagatcgc cagcggcggg
     1261 gcgccgctca gcaaggaggt aggtgaggcc gtggccaaac gcttccacct accaggcatc
     1321 cgccagggct acggcctgac agaaacaacc agcgccattc tgatcacccc cgaaggggac
     1381 gacaagcctg gcgcagtagg caaggtggtg cccttcttcg aggctaaggt ggtggacttg
     1441 gacaccggta agacactggg tgtgaaccag cgcggcgagc tgtgcgtccg tggccccatg
     1501 atcatgagcg gctacgttaa caaccccgag gctacaaacg ctctcatcga caaggacggc
     1561 tggctgcaca gcggcgacat cgcctactgg gacgaggacg agcacttctt catcgtggac
     1621 cggctgaaga gcctgatcaa atacaagggc taccaggtag ccccagccga actggagagc
     1681 atcctgctgc aacaccccaa catcttcgac gccggggtcg ccggcctgcc cgacgacgat
     1741 gccggcgagc tgcccgccgc agtcgtcgtg ctggaacacg gtaaaaccat gaccgagaag
     1801 gagatcgtgg actatgtggc cagccaggtt acaaccgcca agaagctgcg cggtggtgtt
     1861 gtgttcgtgg acgaggtgcc taaaggactg accggcaagt tggacgcccg caagatccgc
     1921 gagattctca ttaaggccaa gaagggcggc aagatcgccg tgtaataatt ctagagtcgg
     1981 ggcggccggc cgcttcgagc agacatgata agatacattg atgagtttgg acaaaccaca
     2041 actagaatgc agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat tgctttattt
     2101 gtaaccatta taagctgcaa taaacaagtt aacaacaaca attgcattca ttttatgttt
     2161 caggttcagg gggaggtgtg ggaggttttt taaagcaagt aaaacctcta caaatgtggt
     2221 aaaatcgata aggatccgtc gaccgatgcc cttgagagcc ttcaacccag tcagctcctt
     2281 ccggtgggcg cggggcatga ctatcgtcgc cgcacttatg actgtcttct ttatcatgca
     2341 actcgtagga caggtgccgg cagcgctctt ccgcttcctc gctcactgac tcgctgcgct
     2401 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
     2461 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
     2521 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
     2581 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
     2641 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
     2701 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
     2761 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
     2821 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
     2881 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
     2941 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg
     3001 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
     3061 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
     3121 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
     3181 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
     3241 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
     3301 ctgacagcgg ccgcaaatgc taaaccactg cagtggttac cagtgcttga tcagtgaggc
     3361 accgatctca gcgatctgcc tatttcgttc gtccatagtg gcctgactcc ccgtcgtgta
     3421 gatcactacg attcgtgagg gcttaccatc aggccccagc gcagcaatga tgccgcgaga
     3481 gccgcgttca ccggcccccg atttgtcagc aatgaaccag ccagcaggga gggccgagcg
     3541 aagaagtggt cctgctactt tgtccgcctc catccagtct atgagctgct gtcgtgatgc
     3601 tagagtaaga agttcgccag tgagtagttt ccgaagagtt gtggccattg ctactggcat
     3661 cgtggtatca cgctcgtcgt tcggtatggc ttcgttcaac tctggttccc agcggtcaag
     3721 ccgggtcaca tgatcaccca tattatgaag aaatgcagtc agctccttag ggcctccgat
     3781 cgttgtcaga agtaagttgg ccgcggtgtt gtcgctcatg gtaatggcag cactacacaa
     3841 ttctcttacc gtcatgccat ccgtaagatg cttttccgtg accggcgagt actcaaccaa
     3901 gtcgttttgt gagtagtgta tacggcgacc aagctgctct tgcccggcgt ctatacggga
     3961 caacaccgcg ccacatagca gtactttgaa agtgctcatc atcgggaatc gttcttcggg
     4021 gcggaaagac tcaaggatct tgccgctatt gagatccagt tcgatatagc ccactcttgc
     4081 acccagttga tcttcagcat cttttacttt caccagcgtt tcggggtgtg caaaaacagg
     4141 caagcaaaat gccgcaaaga agggaatgag tgcgacacga aaatgttgga tgctcatact
     4201 cgtccttttt caatattatt gaagcattta tcagggttac ta
//
