LOCUS       40924_22038        5073 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Reporter vector pGL3casp11pd2, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5073)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman 
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5073)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent, 
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 5073)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5073)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927, 
            9052, BELGIUM"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5073
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    29..64
                     /label=Adaptor sequence
                     /note="Adaptor sequence"
     regulatory      65..283
                     /note="194 bp mCASP-11 promoter fragm."
                     /regulatory_class="promoter"
     CDS             343..1992
                     /label=luciferase
                     /note="firefly luciferase"
     polyA_signal    complement(2036..2157)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(2576..3164)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3338..4195)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(4196..4300)
                     /label=AmpR promoter
     rep_origin      4327..4782
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     polyA_signal    4913..4961
                     /label=poly(A) signal
                     /note="synthetic polyadenylation signal"
     misc_feature    4975..5066
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from
                     the human alpha-2 globin gene"
ORIGIN
        1 ggtaccgagc tcttacgcgt gctagcccac tatagggcac gcgtggtcga cggccctggc
       61 tggtgcttta gttgtgtttg tttgttccca tgacgaagtg ataaaattca ggaaaaggga
      121 ggccagtttt atgagttaca ggggatttcc ccacagcagt tgatactttc tgagcagctc
      181 tttcaacatc tcctggaagt ccccgcctcc ctatgctaag gaaatgccca gtaactttca
      241 ttttactctg tcaagctgtc ttcacagtgc gaaagaactg agggggctcg agatctgcga
      301 tctaagtaag cttggcattc cggtactgtt ggtaaagcca ccatggaaga cgccaaaaac
      361 ataaagaaag gcccggcgcc attctatccg ctggaagatg gaaccgctgg agagcaactg
      421 cataaggcta tgaagagata cgccctggtt cctggaacaa ttgcttttac agatgcacat
      481 atcgaggtgg acatcactta cgctgagtac ttcgaaatgt ccgttcggtt ggcagaagct
      541 atgaaacgat atgggctgaa tacaaatcac agaatcgtcg tatgcagtga aaactctctt
      601 caattcttta tgccggtgtt gggcgcgtta tttatcggag ttgcagttgc gcccgcgaac
      661 gacatttata atgaacgtga attgctcaac agtatgggca tttcgcagcc taccgtggtg
      721 ttcgtttcca aaaaggggtt gcaaaaaatt ttgaacgtgc aaaaaaagct cccaatcatc
      781 caaaaaatta ttatcatgga ttctaaaacg gattaccagg gatttcagtc gatgtacacg
      841 ttcgtcacat ctcatctacc tcccggtttt aatgaatacg attttgtgcc agagtccttc
      901 gatagggaca agacaattgc actgatcatg aactcctctg gatctactgg tctgcctaaa
      961 ggtgtcgctc tgcctcatag aactgcctgc gtgagattct cgcatgccag agatcctatt
     1021 tttggcaatc aaatcattcc ggatactgcg attttaagtg ttgttccatt ccatcacggt
     1081 tttggaatgt ttactacact cggatatttg atatgtggat ttcgagtcgt cttaatgtat
     1141 agatttgaag aagagctgtt tctgaggagc cttcaggatt acaagattca aagtgcgctg
     1201 ctggtgccaa ccctattctc cttcttcgcc aaaagcactc tgattgacaa atacgattta
     1261 tctaatttac acgaaattgc ttctggtggc gctcccctct ctaaggaagt cggggaagcg
     1321 gttgccaaga ggttccatct gccaggtatc aggcaaggat atgggctcac tgagactaca
     1381 tcagctattc tgattacacc cgagggggat gataaaccgg gcgcggtcgg taaagttgtt
     1441 ccattttttg aagcgaaggt tgtggatctg gataccggga aaacgctggg cgttaatcaa
     1501 agaggcgaac tgtgtgtgag aggtcctatg attatgtccg gttatgtaaa caatccggaa
     1561 gcgaccaacg ccttgattga caaggatgga tggctacatt ctggagacat agcttactgg
     1621 gacgaagacg aacacttctt catcgttgac cgcctgaagt ctctgattaa gtacaaaggc
     1681 tatcaggtgg ctcccgctga attggaatcc atcttgctcc aacaccccaa catcttcgac
     1741 gcaggtgtcg caggtcttcc cgacgatgac gccggtgaac ttcccgccgc cgttgttgtt
     1801 ttggagcacg gaaagacgat gacggaaaaa gagatcgtgg attacgtcgc cagtcaagta
     1861 acaaccgcga aaaagttgcg cggaggagtt gtgtttgtgg acgaagtacc gaaaggtctt
     1921 accggaaaac tcgacgcaag aaaaatcaga gagatcctca taaaggccaa gaagggcgga
     1981 aagatcgccg tgtaattcta gagtcggggc ggccggccgc ttcgagcaga catgataaga
     2041 tacattgatg agtttggaca aaccacaact agaatgcagt gaaaaaaatg ctttatttgt
     2101 gaaatttgtg atgctattgc tttatttgta accattataa gctgcaataa acaagttaac
     2161 aacaacaatt gcattcattt tatgtttcag gttcaggggg aggtgtggga ggttttttaa
     2221 agcaagtaaa acctctacaa atgtggtaaa atcgataagg atccgtcgac cgatgccctt
     2281 gagagccttc aacccagtca gctccttccg gtgggcgcgg ggcatgacta tcgtcgccgc
     2341 acttatgact gtcttcttta tcatgcaact cgtaggacag gtgccggcag cgctcttccg
     2401 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc
     2461 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
     2521 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
     2581 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
     2641 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
     2701 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
     2761 cgctttctca atgctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
     2821 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
     2881 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
     2941 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
     3001 acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
     3061 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
     3121 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
     3181 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
     3241 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
     3301 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac
     3361 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga
     3421 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc
     3481 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca
     3541 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta
     3601 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg
     3661 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc
     3721 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg
     3781 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt
     3841 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt
     3901 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata
     3961 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc
     4021 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac
     4081 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa
     4141 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct
     4201 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat
     4261 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc
     4321 cacctgacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg
     4381 tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc
     4441 tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc
     4501 gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta
     4561 gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta
     4621 atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg
     4681 atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa
     4741 aatttaacgc gaattttaac aaaatattaa cgtttacaat ttcccattcg ccattcaggc
     4801 tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagcccaagc
     4861 taccatgata agtaagtaat attaaggtac gggaggtact tggagcggcc gcaataaaat
     4921 atctttattt tcattacatc tgtgtgttgg ttttttgtgt gaatcgatag tactaacata
     4981 cgctctccat caaaacaaaa cgaaacaaaa caaactagca aaataggctg tccccagtgc
     5041 aagtgcaggt gccagaacat ttctctatcg ata
//
