LOCUS       40924_22033        5251 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Reporter vector pGL3casp11pd1, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5251)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman 
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5251)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent, 
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 5251)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5251)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927, 
            9052, BELGIUM"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5251
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      29..461
                     /note="408 bp mCASP-11 promoter fragm."
                     /regulatory_class="promoter"
     CDS             521..2170
                     /label=luciferase
                     /note="firefly luciferase"
     polyA_signal    complement(2214..2335)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(2754..3342)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3516..4373)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(4374..4478)
                     /label=AmpR promoter
     rep_origin      4505..4960
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     polyA_signal    5091..5139
                     /label=poly(A) signal
                     /note="synthetic polyadenylation signal"
     misc_feature    5153..5244
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from
                     the human alpha-2 globin gene"
ORIGIN
        1 ggtaccgagc tcttacgcgt gctagccccg taccttgaat taggaaacaa ttgcttaatt
       61 ttccagtaga aatgaatatg ttaaatttca ttgacttaga actgatgttt tagaaagcag
      121 aggttgagtt actgagaagc cagactagaa tataagcagc actgtgtctt accggagccc
      181 tgaagaatag agaaaatgca aaggtgaaca acttcaattt ttttttttct atttttgcag
      241 ccgctttagt tgtgtttgtt tgttcccatg acgaagtgat aaaattcagg aaaagggagg
      301 ccagttttat gagttacagg ggatttcccc acagcagttg atactttctg agcggctctt
      361 tcaacatctc ctggaagtcc ccgcctccct atgctaagga aatgcccggt aactttcatt
      421 ttactctgtc aagctgtctt cacagtgcga aagaactgag ggggctcgag atctgcgatc
      481 taagtaagct tggcattccg gtactgttgg taaagccacc atggaagacg ccaaaaacat
      541 aaagaaaggc ccggcgccat tctatccgct ggaagatgga accgctggag agcaactgca
      601 taaggctatg aagagatacg ccctggttcc tggaacaatt gcttttacag atgcacatat
      661 cgaggtggac atcacttacg ctgagtactt cgaaatgtcc gttcggttgg cagaagctat
      721 gaaacgatat gggctgaata caaatcacag aatcgtcgta tgcagtgaaa actctcttca
      781 attctttatg ccggtgttgg gcgcgttatt tatcggagtt gcagttgcgc ccgcgaacga
      841 catttataat gaacgtgaat tgctcaacag tatgggcatt tcgcagccta ccgtggtgtt
      901 cgtttccaaa aaggggttgc aaaaaatttt gaacgtgcaa aaaaagctcc caatcatcca
      961 aaaaattatt atcatggatt ctaaaacgga ttaccaggga tttcagtcga tgtacacgtt
     1021 cgtcacatct catctacctc ccggttttaa tgaatacgat tttgtgccag agtccttcga
     1081 tagggacaag acaattgcac tgatcatgaa ctcctctgga tctactggtc tgcctaaagg
     1141 tgtcgctctg cctcatagaa ctgcctgcgt gagattctcg catgccagag atcctatttt
     1201 tggcaatcaa atcattccgg atactgcgat tttaagtgtt gttccattcc atcacggttt
     1261 tggaatgttt actacactcg gatatttgat atgtggattt cgagtcgtct taatgtatag
     1321 atttgaagaa gagctgtttc tgaggagcct tcaggattac aagattcaaa gtgcgctgct
     1381 ggtgccaacc ctattctcct tcttcgccaa aagcactctg attgacaaat acgatttatc
     1441 taatttacac gaaattgctt ctggtggcgc tcccctctct aaggaagtcg gggaagcggt
     1501 tgccaagagg ttccatctgc caggtatcag gcaaggatat gggctcactg agactacatc
     1561 agctattctg attacacccg agggggatga taaaccgggc gcggtcggta aagttgttcc
     1621 attttttgaa gcgaaggttg tggatctgga taccgggaaa acgctgggcg ttaatcaaag
     1681 aggcgaactg tgtgtgagag gtcctatgat tatgtccggt tatgtaaaca atccggaagc
     1741 gaccaacgcc ttgattgaca aggatggatg gctacattct ggagacatag cttactggga
     1801 cgaagacgaa cacttcttca tcgttgaccg cctgaagtct ctgattaagt acaaaggcta
     1861 tcaggtggct cccgctgaat tggaatccat cttgctccaa caccccaaca tcttcgacgc
     1921 aggtgtcgca ggtcttcccg acgatgacgc cggtgaactt cccgccgccg ttgttgtttt
     1981 ggagcacgga aagacgatga cggaaaaaga gatcgtggat tacgtcgcca gtcaagtaac
     2041 aaccgcgaaa aagttgcgcg gaggagttgt gtttgtggac gaagtaccga aaggtcttac
     2101 cggaaaactc gacgcaagaa aaatcagaga gatcctcata aaggccaaga agggcggaaa
     2161 gatcgccgtg taattctaga gtcggggcgg ccggccgctt cgagcagaca tgataagata
     2221 cattgatgag tttggacaaa ccacaactag aatgcagtga aaaaaatgct ttatttgtga
     2281 aatttgtgat gctattgctt tatttgtaac cattataagc tgcaataaac aagttaacaa
     2341 caacaattgc attcatttta tgtttcaggt tcagggggag gtgtgggagg ttttttaaag
     2401 caagtaaaac ctctacaaat gtggtaaaat cgataaggat ccgtcgaccg atgcccttga
     2461 gagccttcaa cccagtcagc tccttccggt gggcgcgggg catgactatc gtcgccgcac
     2521 ttatgactgt cttctttatc atgcaactcg taggacaggt gccggcagcg ctcttccgct
     2581 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
     2641 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
     2701 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
     2761 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
     2821 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
     2881 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
     2941 ctttctcaat gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
     3001 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
     3061 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
     3121 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
     3181 ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
     3241 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
     3301 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
     3361 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
     3421 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
     3481 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
     3541 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
     3601 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
     3661 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
     3721 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
     3781 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
     3841 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
     3901 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
     3961 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
     4021 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
     4081 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
     4141 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
     4201 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
     4261 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
     4321 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
     4381 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
     4441 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
     4501 cctgacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg
     4561 accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc
     4621 gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga
     4681 tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt
     4741 gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat
     4801 agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat
     4861 ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa
     4921 tttaacgcga attttaacaa aatattaacg tttacaattt cccattcgcc attcaggctg
     4981 cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc tattacgcca gcccaagcta
     5041 ccatgataag taagtaatat taaggtacgg gaggtacttg gagcggccgc aataaaatat
     5101 ctttattttc attacatctg tgtgttggtt ttttgtgtga atcgatagta ctaacatacg
     5161 ctctccatca aaacaaaacg aaacaaaaca aactagcaaa ataggctgtc cccagtgcaa
     5221 gtgcaggtgc cagaacattt ctctatcgat a
//
