LOCUS       40924_22028        5805 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Reporter vector pGL3casp11p, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5805)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman 
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5805)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent, 
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 5805)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5805)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927, 
            9052, BELGIUM"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5805
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    29..64
                     /label=adaptor sequence
                     /note="adaptor sequence"
     regulatory      65..990
                     /label=926 bp mCASP-11 promoter fragment
                     /note="926 bp mCASP-11 promoter fragment"
                     /regulatory_class="promoter"
     misc_feature    991..1015
                     /label=5' UTR of mCASP-11
                     /note="5' UTR of mCASP-11"
     CDS             1075..2724
                     /label=luciferase
                     /note="firefly luciferase"
     polyA_signal    complement(2768..2889)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(3308..3896)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4070..4927)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(4928..5032)
                     /label=AmpR promoter
     rep_origin      5059..5514
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     polyA_signal    5645..5693
                     /label=poly(A) signal
                     /note="synthetic polyadenylation signal"
     misc_feature    5707..5798
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from
                     the human alpha-2 globin gene"
ORIGIN
        1 ggtaccgagc tcttacgcgt gctagcccac tctagggcac gcgtggtcga cggcccgggc
       61 tggtattgac ttagcccatg aaccttaaaa tttcacattc tgattgagac tataaaacag
      121 tcagtgccac caacaaccag ggaacaatta ccagataagt atgacactga ggagatgggc
      181 gatagtggca ttactttatc cattctctca gcagtatttg ttaactgtag gaggagctga
      241 agaaggacac tttcacaaga gcctttctaa tccttcctat ttaatccaac gttccccaaa
      301 gactcaaagt tgaacacacc acagcatttg tcttttcccc tttctctaac cattcccctg
      361 acatacctct ctcctcagat catgtgatca tgcattcatt atattgcacc gtgcaagtgc
      421 ccctatttgt tcaagaggct acgaacaggt agtagtaatc atcactctca gtaaaaattt
      481 gtcttgcaca attaatgaaa tgcacccagg gtggacaaaa ggattgctct gaactatgtc
      541 taataaaaac gaggtctgta gaaatatgta tagaaaggct gtagtacctt gaattaggaa
      601 acaattgctt aattttccag tagaaatgaa tatgttaaat ttcattgact tagaactgat
      661 gttttagaaa gcagaggttg agttactgag aagccagact agaatataag cagcactgtg
      721 tcttactgga gccctgaaga gtagagaaaa tgcaaaggta aacaacttca attttttttt
      781 ttctattttt gcagccgctt tagttgtgtt tgtttgttcc catgacgaag tgataaaatt
      841 caggaaaagg gaggccagtt ttatgagtta caggggattt ccccacagca gttgatactt
      901 tctgagcagc tctttcaaca tctcctggaa gtccccgcct ccctatgcta aggaaatgcc
      961 cagtaacttt cattttactc tgtcaagctg tcttcacagt gcgaaagaac tgagggggct
     1021 cgagatctgc gatctaagta agcttggcat tccggtactg ttggtaaagc caccatggaa
     1081 gacgccaaaa acataaagaa aggcccggcg ccattctatc cgctggaaga tggaaccgct
     1141 ggagagcaac tgcataaggc tatgaagaga tacgccctgg ttcctggaac aattgctttt
     1201 acagatgcac atatcgaggt ggacatcact tacgctgagt acttcgaaat gtccgttcgg
     1261 ttggcagaag ctatgaaacg atatgggctg aatacaaatc acagaatcgt cgtatgcagt
     1321 gaaaactctc ttcaattctt tatgccggtg ttgggcgcgt tatttatcgg agttgcagtt
     1381 gcgcccgcga acgacattta taatgaacgt gaattgctca acagtatggg catttcgcag
     1441 cctaccgtgg tgttcgtttc caaaaagggg ttgcaaaaaa ttttgaacgt gcaaaaaaag
     1501 ctcccaatca tccaaaaaat tattatcatg gattctaaaa cggattacca gggatttcag
     1561 tcgatgtaca cgttcgtcac atctcatcta cctcccggtt ttaatgaata cgattttgtg
     1621 ccagagtcct tcgataggga caagacaatt gcactgatca tgaactcctc tggatctact
     1681 ggtctgccta aaggtgtcgc tctgcctcat agaactgcct gcgtgagatt ctcgcatgcc
     1741 agagatccta tttttggcaa tcaaatcatt ccggatactg cgattttaag tgttgttcca
     1801 ttccatcacg gttttggaat gtttactaca ctcggatatt tgatatgtgg atttcgagtc
     1861 gtcttaatgt atagatttga agaagagctg tttctgagga gccttcagga ttacaagatt
     1921 caaagtgcgc tgctggtgcc aaccctattc tccttcttcg ccaaaagcac tctgattgac
     1981 aaatacgatt tatctaattt acacgaaatt gcttctggtg gcgctcccct ctctaaggaa
     2041 gtcggggaag cggttgccaa gaggttccat ctgccaggta tcaggcaagg atatgggctc
     2101 actgagacta catcagctat tctgattaca cccgaggggg atgataaacc gggcgcggtc
     2161 ggtaaagttg ttccattttt tgaagcgaag gttgtggatc tggataccgg gaaaacgctg
     2221 ggcgttaatc aaagaggcga actgtgtgtg agaggtccta tgattatgtc cggttatgta
     2281 aacaatccgg aagcgaccaa cgccttgatt gacaaggatg gatggctaca ttctggagac
     2341 atagcttact gggacgaaga cgaacacttc ttcatcgttg accgcctgaa gtctctgatt
     2401 aagtacaaag gctatcaggt ggctcccgct gaattggaat ccatcttgct ccaacacccc
     2461 aacatcttcg acgcaggtgt cgcaggtctt cccgacgatg acgccggtga acttcccgcc
     2521 gccgttgttg ttttggagca cggaaagacg atgacggaaa aagagatcgt ggattacgtc
     2581 gccagtcaag taacaaccgc gaaaaagttg cgcggaggag ttgtgtttgt ggacgaagta
     2641 ccgaaaggtc ttaccggaaa actcgacgca agaaaaatca gagagatcct cataaaggcc
     2701 aagaagggcg gaaagatcgc cgtgtaattc tagagtcggg gcggccggcc gcttcgagca
     2761 gacatgataa gatacattga tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa
     2821 tgctttattt gtgaaatttg tgatgctatt gctttatttg taaccattat aagctgcaat
     2881 aaacaagtta acaacaacaa ttgcattcat tttatgtttc aggttcaggg ggaggtgtgg
     2941 gaggtttttt aaagcaagta aaacctctac aaatgtggta aaatcgataa ggatccgtcg
     3001 accgatgccc ttgagagcct tcaacccagt cagctccttc cggtgggcgc ggggcatgac
     3061 tatcgtcgcc gcacttatga ctgtcttctt tatcatgcaa ctcgtaggac aggtgccggc
     3121 agcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
     3181 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
     3241 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
     3301 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
     3361 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
     3421 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
     3481 cgggaagcgt ggcgctttct caatgctcac gctgtaggta tctcagttcg gtgtaggtcg
     3541 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
     3601 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
     3661 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
     3721 ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc
     3781 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
     3841 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
     3901 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
     3961 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
     4021 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
     4081 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
     4141 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
     4201 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
     4261 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
     4321 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg
     4381 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
     4441 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
     4501 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
     4561 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
     4621 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
     4681 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
     4741 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
     4801 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
     4861 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
     4921 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
     4981 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
     5041 cccgaaaagt gccacctgac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg
     5101 ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct
     5161 tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc
     5221 ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg
     5281 atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt
     5341 ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg
     5401 tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc
     5461 tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgtttaca atttcccatt
     5521 cgccattcag gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct tcgctattac
     5581 gccagcccaa gctaccatga taagtaagta atattaaggt acgggaggta cttggagcgg
     5641 ccgcaataaa atatctttat tttcattaca tctgtgtgtt ggttttttgt gtgaatcgat
     5701 agtactaaca tacgctctcc atcaaaacaa aacgaaacaa aacaaactag caaaataggc
     5761 tgtccccagt gcaagtgcag gtgccagaac atttctctat cgata
//
