LOCUS       40924_22018        4859 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Reporter vector pGL3casp11p-Del7, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4859)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman 
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4859)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent, 
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 4859)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4859)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927, 
            9052, BELGIUM"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4859
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      21..126
                     /note="81 bp mCASP-11 promoter fragm."
                     /regulatory_class="promoter"
     CDS             129..1778
                     /label=luciferase
                     /note="firefly luciferase"
     polyA_signal    complement(1822..1943)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(2362..2950)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3124..3981)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(3982..4086)
                     /label=AmpR promoter
     rep_origin      4113..4568
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     polyA_signal    4699..4747
                     /label=poly(A) signal
                     /note="synthetic polyadenylation signal"
     misc_feature    4761..4852
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from
                     the human alpha-2 globin gene"
ORIGIN
        1 ggtaccgagc tcttacgcgt ctctttcaac atctcctgga agtccccgcc tccctatgct
       61 aaggaaatgc ccagtaactt tcattttact ctgtcaagct gtcttcacag tgcgaaagaa
      121 ctgaggccat ggaagacgcc aaaaacataa agaaaggccc ggcgccattc tatccgctgg
      181 aagatggaac cgctggagag caactgcata aggctatgaa gagatacgcc ctggttcctg
      241 gaacaattgc ttttacagat gcacatatcg aggtggacat cacttacgct gagtacttcg
      301 aaatgtccgt tcggttggca gaagctatga aacgatatgg gctgaataca aatcacagaa
      361 tcgtcgtatg cagtgaaaac tctcttcaat tctttatgcc ggtgttgggc gcgttattta
      421 tcggagttgc agttgcgccc gcgaacgaca tttataatga acgtgaattg ctcaacagta
      481 tgggcatttc gcagcctacc gtggtgttcg tttccaaaaa ggggttgcaa aaaattttga
      541 acgtgcaaaa aaagctccca atcatccaaa aaattattat catggattct aaaacggatt
      601 accagggatt tcagtcgatg tacacgttcg tcacatctca tctacctccc ggttttaatg
      661 aatacgattt tgtgccagag tccttcgata gggacaagac aattgcactg atcatgaact
      721 cctctggatc tactggtctg cctaaaggtg tcgctctgcc tcatagaact gcctgcgtga
      781 gattctcgca tgccagagat cctatttttg gcaatcaaat cattccggat actgcgattt
      841 taagtgttgt tccattccat cacggttttg gaatgtttac tacactcgga tatttgatat
      901 gtggatttcg agtcgtctta atgtatagat ttgaagaaga gctgtttctg aggagccttc
      961 aggattacaa gattcaaagt gcgctgctgg tgccaaccct attctccttc ttcgccaaaa
     1021 gcactctgat tgacaaatac gatttatcta atttacacga aattgcttct ggtggcgctc
     1081 ccctctctaa ggaagtcggg gaagcggttg ccaagaggtt ccatctgcca ggtatcaggc
     1141 aaggatatgg gctcactgag actacatcag ctattctgat tacacccgag ggggatgata
     1201 aaccgggcgc ggtcggtaaa gttgttccat tttttgaagc gaaggttgtg gatctggata
     1261 ccgggaaaac gctgggcgtt aatcaaagag gcgaactgtg tgtgagaggt cctatgatta
     1321 tgtccggtta tgtaaacaat ccggaagcga ccaacgcctt gattgacaag gatggatggc
     1381 tacattctgg agacatagct tactgggacg aagacgaaca cttcttcatc gttgaccgcc
     1441 tgaagtctct gattaagtac aaaggctatc aggtggctcc cgctgaattg gaatccatct
     1501 tgctccaaca ccccaacatc ttcgacgcag gtgtcgcagg tcttcccgac gatgacgccg
     1561 gtgaacttcc cgccgccgtt gttgttttgg agcacggaaa gacgatgacg gaaaaagaga
     1621 tcgtggatta cgtcgccagt caagtaacaa ccgcgaaaaa gttgcgcgga ggagttgtgt
     1681 ttgtggacga agtaccgaaa ggtcttaccg gaaaactcga cgcaagaaaa atcagagaga
     1741 tcctcataaa ggccaagaag ggcggaaaga tcgccgtgta attctagagt cggggcggcc
     1801 ggccgcttcg agcagacatg ataagataca ttgatgagtt tggacaaacc acaactagaa
     1861 tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta tttgtaacca
     1921 ttataagctg caataaacaa gttaacaaca acaattgcat tcattttatg tttcaggttc
     1981 agggggaggt gtgggaggtt ttttaaagca agtaaaacct ctacaaatgt ggtaaaatcg
     2041 ataaggatcc gtcgaccgat gcccttgaga gccttcaacc cagtcagctc cttccggtgg
     2101 gcgcggggca tgactatcgt cgccgcactt atgactgtct tctttatcat gcaactcgta
     2161 ggacaggtgc cggcagcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt
     2221 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
     2281 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
     2341 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
     2401 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
     2461 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
     2521 cgcctttctc ccttcgggaa gcgtggcgct ttctcaatgc tcacgctgta ggtatctcag
     2581 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
     2641 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
     2701 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
     2761 agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg
     2821 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
     2881 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
     2941 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
     3001 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
     3061 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag
     3121 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
     3181 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
     3241 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa
     3301 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
     3361 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
     3421 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
     3481 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
     3541 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
     3601 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
     3661 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
     3721 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
     3781 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
     3841 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
     3901 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
     3961 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
     4021 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
     4081 tccgcgcaca tttccccgaa aagtgccacc tgacgcgccc tgtagcggcg cattaagcgc
     4141 ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc
     4201 tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct
     4261 aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa
     4321 acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc
     4381 tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact
     4441 caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg
     4501 gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgtt
     4561 tacaatttcc cattcgccat tcaggctgcg caactgttgg gaagggcgat cggtgcgggc
     4621 ctcttcgcta ttacgccagc ccaagctacc atgataagta agtaatatta aggtacggga
     4681 ggtacttgga gcggccgcaa taaaatatct ttattttcat tacatctgtg tgttggtttt
     4741 ttgtgtgaat cgatagtact aacatacgct ctccatcaaa acaaaacgaa acaaaacaaa
     4801 ctagcaaaat aggctgtccc cagtgcaagt gcaggtgcca gaacatttct ctatcgata
//
