LOCUS       40924_22003        4916 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Reporter vector pGL3casp11p-Del4, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4916)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman 
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4916)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent, 
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 4916)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4916)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927, 
            9052, BELGIUM"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4916
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      21..183
                     /note="138 bp mCASP-11 promoter fragm."
                     /regulatory_class="promoter"
     CDS             186..1835
                     /label=luciferase
                     /note="firefly luciferase"
     polyA_signal    complement(1879..2000)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(2419..3007)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3181..4038)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(4039..4143)
                     /label=AmpR promoter
     rep_origin      4170..4625
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     polyA_signal    4756..4804
                     /label=poly(A) signal
                     /note="synthetic polyadenylation signal"
     misc_feature    4818..4909
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from
                     the human alpha-2 globin gene"
ORIGIN
        1 ggtaccgagc tcttacgcgt ggccagtttt atgagttaca ggggatttcc ccacagcagt
       61 tgatactttc tgagcagctc tttcaacatc tcctggaagt ccccgcctcc ctatgctaag
      121 gaaatgccca gtaactttca ttttactctg tcaagctgtc ttcacagtgc gaaagaactg
      181 aggccatgga agacgccaaa aacataaaga aaggcccggc gccattctat ccgctggaag
      241 atggaaccgc tggagagcaa ctgcataagg ctatgaagag atacgccctg gttcctggaa
      301 caattgcttt tacagatgca catatcgagg tggacatcac ttacgctgag tacttcgaaa
      361 tgtccgttcg gttggcagaa gctatgaaac gatatgggct gaatacaaat cacagaatcg
      421 tcgtatgcag tgaaaactct cttcaattct ttatgccggt gttgggcgcg ttatttatcg
      481 gagttgcagt tgcgcccgcg aacgacattt ataatgaacg tgaattgctc aacagtatgg
      541 gcatttcgca gcctaccgtg gtgttcgttt ccaaaaaggg gttgcaaaaa attttgaacg
      601 tgcaaaaaaa gctcccaatc atccaaaaaa ttattatcat ggattctaaa acggattacc
      661 agggatttca gtcgatgtac acgttcgtca catctcatct acctcccggt tttaatgaat
      721 acgattttgt gccagagtcc ttcgataggg acaagacaat tgcactgatc atgaactcct
      781 ctggatctac tggtctgcct aaaggtgtcg ctctgcctca tagaactgcc tgcgtgagat
      841 tctcgcatgc cagagatcct atttttggca atcaaatcat tccggatact gcgattttaa
      901 gtgttgttcc attccatcac ggttttggaa tgtttactac actcggatat ttgatatgtg
      961 gatttcgagt cgtcttaatg tatagatttg aagaagagct gtttctgagg agccttcagg
     1021 attacaagat tcaaagtgcg ctgctggtgc caaccctatt ctccttcttc gccaaaagca
     1081 ctctgattga caaatacgat ttatctaatt tacacgaaat tgcttctggt ggcgctcccc
     1141 tctctaagga agtcggggaa gcggttgcca agaggttcca tctgccaggt atcaggcaag
     1201 gatatgggct cactgagact acatcagcta ttctgattac acccgagggg gatgataaac
     1261 cgggcgcggt cggtaaagtt gttccatttt ttgaagcgaa ggttgtggat ctggataccg
     1321 ggaaaacgct gggcgttaat caaagaggcg aactgtgtgt gagaggtcct atgattatgt
     1381 ccggttatgt aaacaatccg gaagcgacca acgccttgat tgacaaggat ggatggctac
     1441 attctggaga catagcttac tgggacgaag acgaacactt cttcatcgtt gaccgcctga
     1501 agtctctgat taagtacaaa ggctatcagg tggctcccgc tgaattggaa tccatcttgc
     1561 tccaacaccc caacatcttc gacgcaggtg tcgcaggtct tcccgacgat gacgccggtg
     1621 aacttcccgc cgccgttgtt gttttggagc acggaaagac gatgacggaa aaagagatcg
     1681 tggattacgt cgccagtcaa gtaacaaccg cgaaaaagtt gcgcggagga gttgtgtttg
     1741 tggacgaagt accgaaaggt cttaccggaa aactcgacgc aagaaaaatc agagagatcc
     1801 tcataaaggc caagaagggc ggaaagatcg ccgtgtaatt ctagagtcgg ggcggccggc
     1861 cgcttcgagc agacatgata agatacattg atgagtttgg acaaaccaca actagaatgc
     1921 agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat tgctttattt gtaaccatta
     1981 taagctgcaa taaacaagtt aacaacaaca attgcattca ttttatgttt caggttcagg
     2041 gggaggtgtg ggaggttttt taaagcaagt aaaacctcta caaatgtggt aaaatcgata
     2101 aggatccgtc gaccgatgcc cttgagagcc ttcaacccag tcagctcctt ccggtgggcg
     2161 cggggcatga ctatcgtcgc cgcacttatg actgtcttct ttatcatgca actcgtagga
     2221 caggtgccgg cagcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
     2281 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
     2341 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
     2401 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
     2461 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     2521 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
     2581 ctttctccct tcgggaagcg tggcgctttc tcaatgctca cgctgtaggt atctcagttc
     2641 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
     2701 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
     2761 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
     2821 gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc
     2881 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
     2941 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
     3001 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
     3061 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
     3121 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
     3181 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
     3241 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
     3301 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca
     3361 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
     3421 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
     3481 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
     3541 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
     3601 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
     3661 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
     3721 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
     3781 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat
     3841 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
     3901 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
     3961 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
     4021 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
     4081 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
     4141 gcgcacattt ccccgaaaag tgccacctga cgcgccctgt agcggcgcat taagcgcggc
     4201 gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc
     4261 tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa
     4321 tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact
     4381 tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt
     4441 gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa
     4501 ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt
     4561 aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgtttac
     4621 aatttcccat tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc
     4681 ttcgctatta cgccagccca agctaccatg ataagtaagt aatattaagg tacgggaggt
     4741 acttggagcg gccgcaataa aatatcttta ttttcattac atctgtgtgt tggttttttg
     4801 tgtgaatcga tagtactaac atacgctctc catcaaaaca aaacgaaaca aaacaaacta
     4861 gcaaaatagg ctgtccccag tgcaagtgca ggtgccagaa catttctcta tcgata
//
