LOCUS       40924_21993        4958 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Reporter vector pGL3casp11p-Del2, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4958)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman 
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4958)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent, 
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 4958)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4958)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927, 
            9052, BELGIUM"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4958
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      21..225
                     /note="180 bp mCASP-11 promoter fragm."
                     /regulatory_class="promoter"
     CDS             228..1877
                     /label=luciferase
                     /note="firefly luciferase"
     polyA_signal    complement(1921..2042)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(2461..3049)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3223..4080)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(4081..4185)
                     /label=AmpR promoter
     rep_origin      4212..4667
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     polyA_signal    4798..4846
                     /label=poly(A) signal
                     /note="synthetic polyadenylation signal"
     misc_feature    4860..4951
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from
                     the human alpha-2 globin gene"
ORIGIN
        1 ggtaccgagc tcttacgcgt tgtttgttcc catgacgaag tgataaaatt caggaaaagg
       61 gaggccagtt ttatgagtta caggggattt ccccacagca gttgatactt tctgagcagc
      121 tctttcaaca tctcctggaa gtccccgcct ccctatgcta aggaaatgcc cagtaacttt
      181 cattttactc tgtcaagctg tcttcacagt gcgaaagaac tgaggccatg gaagacgcca
      241 aaaacataaa gaaaggcccg gcgccattct atccgctgga agatggaacc gctggagagc
      301 aactgcataa ggctatgaag agatacgccc tggttcctgg aacaattgct tttacagatg
      361 cacatatcga ggtggacatc acttacgctg agtacttcga aatgtccgtt cggttggcag
      421 aagctatgaa acgatatggg ctgaatacaa atcacagaat cgtcgtatgc agtgaaaact
      481 ctcttcaatt ctttatgccg gtgttgggcg cgttatttat cggagttgca gttgcgcccg
      541 cgaacgacat ttataatgaa cgtgaattgc tcaacagtat gggcatttcg cagcctaccg
      601 tggtgttcgt ttccaaaaag gggttgcaaa aaattttgaa cgtgcaaaaa aagctcccaa
      661 tcatccaaaa aattattatc atggattcta aaacggatta ccagggattt cagtcgatgt
      721 acacgttcgt cacatctcat ctacctcccg gttttaatga atacgatttt gtgccagagt
      781 ccttcgatag ggacaagaca attgcactga tcatgaactc ctctggatct actggtctgc
      841 ctaaaggtgt cgctctgcct catagaactg cctgcgtgag attctcgcat gccagagatc
      901 ctatttttgg caatcaaatc attccggata ctgcgatttt aagtgttgtt ccattccatc
      961 acggttttgg aatgtttact acactcggat atttgatatg tggatttcga gtcgtcttaa
     1021 tgtatagatt tgaagaagag ctgtttctga ggagccttca ggattacaag attcaaagtg
     1081 cgctgctggt gccaacccta ttctccttct tcgccaaaag cactctgatt gacaaatacg
     1141 atttatctaa tttacacgaa attgcttctg gtggcgctcc cctctctaag gaagtcgggg
     1201 aagcggttgc caagaggttc catctgccag gtatcaggca aggatatggg ctcactgaga
     1261 ctacatcagc tattctgatt acacccgagg gggatgataa accgggcgcg gtcggtaaag
     1321 ttgttccatt ttttgaagcg aaggttgtgg atctggatac cgggaaaacg ctgggcgtta
     1381 atcaaagagg cgaactgtgt gtgagaggtc ctatgattat gtccggttat gtaaacaatc
     1441 cggaagcgac caacgccttg attgacaagg atggatggct acattctgga gacatagctt
     1501 actgggacga agacgaacac ttcttcatcg ttgaccgcct gaagtctctg attaagtaca
     1561 aaggctatca ggtggctccc gctgaattgg aatccatctt gctccaacac cccaacatct
     1621 tcgacgcagg tgtcgcaggt cttcccgacg atgacgccgg tgaacttccc gccgccgttg
     1681 ttgttttgga gcacggaaag acgatgacgg aaaaagagat cgtggattac gtcgccagtc
     1741 aagtaacaac cgcgaaaaag ttgcgcggag gagttgtgtt tgtggacgaa gtaccgaaag
     1801 gtcttaccgg aaaactcgac gcaagaaaaa tcagagagat cctcataaag gccaagaagg
     1861 gcggaaagat cgccgtgtaa ttctagagtc ggggcggccg gccgcttcga gcagacatga
     1921 taagatacat tgatgagttt ggacaaacca caactagaat gcagtgaaaa aaatgcttta
     1981 tttgtgaaat ttgtgatgct attgctttat ttgtaaccat tataagctgc aataaacaag
     2041 ttaacaacaa caattgcatt cattttatgt ttcaggttca gggggaggtg tgggaggttt
     2101 tttaaagcaa gtaaaacctc tacaaatgtg gtaaaatcga taaggatccg tcgaccgatg
     2161 cccttgagag ccttcaaccc agtcagctcc ttccggtggg cgcggggcat gactatcgtc
     2221 gccgcactta tgactgtctt ctttatcatg caactcgtag gacaggtgcc ggcagcgctc
     2281 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
     2341 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
     2401 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     2461 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     2521 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     2581 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     2641 cgtggcgctt tctcaatgct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     2701 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     2761 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     2821 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     2881 taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac
     2941 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
     3001 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     3061 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
     3121 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
     3181 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
     3241 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
     3301 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
     3361 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
     3421 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
     3481 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
     3541 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
     3601 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
     3661 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
     3721 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
     3781 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
     3841 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
     3901 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
     3961 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
     4021 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
     4081 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
     4141 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
     4201 agtgccacct gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg
     4261 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc
     4321 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg
     4381 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc
     4441 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt
     4501 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc
     4561 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta
     4621 acaaaaattt aacgcgaatt ttaacaaaat attaacgttt acaatttccc attcgccatt
     4681 caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat tacgccagcc
     4741 caagctacca tgataagtaa gtaatattaa ggtacgggag gtacttggag cggccgcaat
     4801 aaaatatctt tattttcatt acatctgtgt gttggttttt tgtgtgaatc gatagtacta
     4861 acatacgctc tccatcaaaa caaaacgaaa caaaacaaac tagcaaaata ggctgtcccc
     4921 agtgcaagtg caggtgccag aacatttctc tatcgata
//
