LOCUS       40924_21988        4978 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Reporter vector pGL3casp11p-Del1, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4978)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman 
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4978)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent, 
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 4978)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4978)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927, 
            9052, BELGIUM"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4978
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      20..220
                     /label=201 bp mCASP-11 promoter fragment
                     /note="201 bp mCASP-11 promoter fragment"
                     /regulatory_class="promoter"
     misc_feature    221..245
                     /label=5' UTR of mCASP-11
                     /note="5' UTR of mCASP-11"
     CDS             248..1897
                     /label=luciferase
                     /note="firefly luciferase"
     polyA_signal    complement(1941..2062)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(2481..3069)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3243..4100)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(4101..4205)
                     /label=AmpR promoter
     rep_origin      4232..4687
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     polyA_signal    4818..4866
                     /label=poly(A) signal
                     /note="synthetic polyadenylation signal"
     misc_feature    4880..4971
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from
                     the human alpha-2 globin gene"
ORIGIN
        1 ggtaccgagc tcttacgcgt gcagccgctt tagttgtgtt tgtttgttcc catgacgaag
       61 tgataaaatt caggaaaagg gaggccagtt ttatgagtta caggggattt ccccacagca
      121 gttgatactt tctgagcagc tctttcaaca tctcctggaa gtccccgcct ccctatgcta
      181 aggaaatgcc cagtaacttt cattttactc tgtcaagctg tcttcacagt gcgaaagaac
      241 tgaggccatg gaagacgcca aaaacataaa gaaaggcccg gcgccattct atccgctgga
      301 agatggaacc gctggagagc aactgcataa ggctatgaag agatacgccc tggttcctgg
      361 aacaattgct tttacagatg cacatatcga ggtggacatc acttacgctg agtacttcga
      421 aatgtccgtt cggttggcag aagctatgaa acgatatggg ctgaatacaa atcacagaat
      481 cgtcgtatgc agtgaaaact ctcttcaatt ctttatgccg gtgttgggcg cgttatttat
      541 cggagttgca gttgcgcccg cgaacgacat ttataatgaa cgtgaattgc tcaacagtat
      601 gggcatttcg cagcctaccg tggtgttcgt ttccaaaaag gggttgcaaa aaattttgaa
      661 cgtgcaaaaa aagctcccaa tcatccaaaa aattattatc atggattcta aaacggatta
      721 ccagggattt cagtcgatgt acacgttcgt cacatctcat ctacctcccg gttttaatga
      781 atacgatttt gtgccagagt ccttcgatag ggacaagaca attgcactga tcatgaactc
      841 ctctggatct actggtctgc ctaaaggtgt cgctctgcct catagaactg cctgcgtgag
      901 attctcgcat gccagagatc ctatttttgg caatcaaatc attccggata ctgcgatttt
      961 aagtgttgtt ccattccatc acggttttgg aatgtttact acactcggat atttgatatg
     1021 tggatttcga gtcgtcttaa tgtatagatt tgaagaagag ctgtttctga ggagccttca
     1081 ggattacaag attcaaagtg cgctgctggt gccaacccta ttctccttct tcgccaaaag
     1141 cactctgatt gacaaatacg atttatctaa tttacacgaa attgcttctg gtggcgctcc
     1201 cctctctaag gaagtcgggg aagcggttgc caagaggttc catctgccag gtatcaggca
     1261 aggatatggg ctcactgaga ctacatcagc tattctgatt acacccgagg gggatgataa
     1321 accgggcgcg gtcggtaaag ttgttccatt ttttgaagcg aaggttgtgg atctggatac
     1381 cgggaaaacg ctgggcgtta atcaaagagg cgaactgtgt gtgagaggtc ctatgattat
     1441 gtccggttat gtaaacaatc cggaagcgac caacgccttg attgacaagg atggatggct
     1501 acattctgga gacatagctt actgggacga agacgaacac ttcttcatcg ttgaccgcct
     1561 gaagtctctg attaagtaca aaggctatca ggtggctccc gctgaattgg aatccatctt
     1621 gctccaacac cccaacatct tcgacgcagg tgtcgcaggt cttcccgacg atgacgccgg
     1681 tgaacttccc gccgccgttg ttgttttgga gcacggaaag acgatgacgg aaaaagagat
     1741 cgtggattac gtcgccagtc aagtaacaac cgcgaaaaag ttgcgcggag gagttgtgtt
     1801 tgtggacgaa gtaccgaaag gtcttaccgg aaaactcgac gcaagaaaaa tcagagagat
     1861 cctcataaag gccaagaagg gcggaaagat cgccgtgtaa ttctagagtc ggggcggccg
     1921 gccgcttcga gcagacatga taagatacat tgatgagttt ggacaaacca caactagaat
     1981 gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct attgctttat ttgtaaccat
     2041 tataagctgc aataaacaag ttaacaacaa caattgcatt cattttatgt ttcaggttca
     2101 gggggaggtg tgggaggttt tttaaagcaa gtaaaacctc tacaaatgtg gtaaaatcga
     2161 taaggatccg tcgaccgatg cccttgagag ccttcaaccc agtcagctcc ttccggtggg
     2221 cgcggggcat gactatcgtc gccgcactta tgactgtctt ctttatcatg caactcgtag
     2281 gacaggtgcc ggcagcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt
     2341 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
     2401 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
     2461 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
     2521 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
     2581 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
     2641 gcctttctcc cttcgggaag cgtggcgctt tctcaatgct cacgctgtag gtatctcagt
     2701 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
     2761 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
     2821 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
     2881 gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc
     2941 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
     3001 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
     3061 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
     3121 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
     3181 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt
     3241 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata
     3301 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc
     3361 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac
     3421 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag
     3481 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac
     3541 gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc
     3601 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg
     3661 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc
     3721 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct
     3781 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc
     3841 tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc
     3901 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc
     3961 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc
     4021 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca
     4081 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt
     4141 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt
     4201 ccgcgcacat ttccccgaaa agtgccacct gacgcgccct gtagcggcgc attaagcgcg
     4261 gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct
     4321 cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta
     4381 aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa
     4441 cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct
     4501 ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc
     4561 aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg
     4621 ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgttt
     4681 acaatttccc attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc
     4741 tcttcgctat tacgccagcc caagctacca tgataagtaa gtaatattaa ggtacgggag
     4801 gtacttggag cggccgcaat aaaatatctt tattttcatt acatctgtgt gttggttttt
     4861 tgtgtgaatc gatagtacta acatacgctc tccatcaaaa caaaacgaaa caaaacaaac
     4921 tagcaaaata ggctgtcccc agtgcaagtg caggtgccag aacatttctc tatcgata
//
