LOCUS       40924_22083        4942 bp DNA     circular SYN 13-MAY-2021
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4942)
  AUTHORS   Korchynskyi O, ten Dijke P
  TITLE     Identification and functional characterization of distinct 
            critically important bone morphogenetic protein-specific response 
            elements in the Id1 promoter.
  JOURNAL   J Biol Chem. 2002 Feb 15;277(7):4883-91. Epub 2001 Nov 29.
  PUBMED    11729207
REFERENCE   2  (bases 1 to 4942)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 4942)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol
            Chem."; date: "2002-02-15"; volume: "277(7)"; pages: "4883-91. Epub
            2001 Nov 29"
COMMENT     SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4942
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             161..1810
                     /codon_start=1
                     /label=luciferase
                     /note="firefly luciferase"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     polyA_signal    complement(1854..1975)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2223..2240)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2394..2982)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3156..4013)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(4014..4118)
                     /label=AmpR promoter
     rep_origin      4145..4600
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     polyA_signal    4731..4779
                     /label=poly(A) signal
                     /note="synthetic polyadenylation signal"
     misc_feature    4793..4884
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from
                     the human alpha-2 globin gene"
ORIGIN
        1 ctgtcaggct ggcgccgcgg cgccagcctg acagcccgtc ctggcgtcta acggtctgag
       61 ctagcccggg ctcgagatct gggctataaa agggggtggg ggcgcgttcg tcctcactct
      121 cttccaagct tggcattccg gtactgttgg taaagccacc atggaagacg ccaaaaacat
      181 aaagaaaggc ccggcgccat tctatccgct ggaagatgga accgctggag agcaactgca
      241 taaggctatg aagagatacg ccctggttcc tggaacaatt gcttttacag atgcacatat
      301 cgaggtggac atcacttacg ctgagtactt cgaaatgtcc gttcggttgg cagaagctat
      361 gaaacgatat gggctgaata caaatcacag aatcgtcgta tgcagtgaaa actctcttca
      421 attctttatg ccggtgttgg gcgcgttatt tatcggagtt gcagttgcgc ccgcgaacga
      481 catttataat gaacgtgaat tgctcaacag tatgggcatt tcgcagccta ccgtggtgtt
      541 cgtttccaaa aaggggttgc aaaaaatttt gaacgtgcaa aaaaagctcc caatcatcca
      601 aaaaattatt atcatggatt ctaaaacgga ttaccaggga tttcagtcga tgtacacgtt
      661 cgtcacatct catctacctc ccggttttaa tgaatacgat tttgtgccag agtccttcga
      721 tagggacaag acaattgcac tgatcatgaa ctcctctgga tctactggtc tgcctaaagg
      781 tgtcgctctg cctcatagaa ctgcctgcgt gagattctcg catgccagag atcctatttt
      841 tggcaatcaa atcattccgg atactgcgat tttaagtgtt gttccattcc atcacggttt
      901 tggaatgttt actacactcg gatatttgat atgtggattt cgagtcgtct taatgtatag
      961 atttgaagaa gagctgtttc tgaggagcct tcaggattac aagattcaaa gtgcgctgct
     1021 ggtgccaacc ctattctcct tcttcgccaa aagcactctg attgacaaat acgatttatc
     1081 taatttacac gaaattgctt ctggtggcgc tcccctctct aaggaagtcg gggaagcggt
     1141 tgccaagagg ttccatctgc caggtatcag gcaaggatat gggctcactg agactacatc
     1201 agctattctg attacacccg agggggatga taaaccgggc gcggtcggta aagttgttcc
     1261 attttttgaa gcgaaggttg tggatctgga taccgggaaa acgctgggcg ttaatcaaag
     1321 aggcgaactg tgtgtgagag gtcctatgat tatgtccggt tatgtaaaca atccggaagc
     1381 gaccaacgcc ttgattgaca aggatggatg gctacattct ggagacatag cttactggga
     1441 cgaagacgaa cacttcttca tcgttgaccg cctgaagtct ctgattaagt acaaaggcta
     1501 tcaggtggct cccgctgaat tggaatccat cttgctccaa caccccaaca tcttcgacgc
     1561 aggtgtcgca ggtcttcccg acgatgacgc cggtgaactt cccgccgccg ttgttgtttt
     1621 ggagcacgga aagacgatga cggaaaaaga gatcgtggat tacgtcgcca gtcaagtaac
     1681 aaccgcgaaa aagttgcgcg gaggagttgt gtttgtggac gaagtaccga aaggtcttac
     1741 cggaaaactc gacgcaagaa aaatcagaga gatcctcata aaggccaaga agggcggaaa
     1801 gatcgccgtg taattctaga gtcggggcgg ccggccgctt cgagcagaca tgataagata
     1861 cattgatgag tttggacaaa ccacaactag aatgcagtga aaaaaatgct ttatttgtga
     1921 aatttgtgat gctattgctt tatttgtaac cattataagc tgcaataaac aagttaacaa
     1981 caacaattgc attcatttta tgtttcaggt tcagggggag gtgtgggagg ttttttaaag
     2041 caagtaaaac ctctacaaat gtggtaaaat cgataaggat ccgtcgaccg atgcccttga
     2101 gagccttcaa cccagtcagc tccttccggt gggcgcgggg catgactatc gtcgccgcac
     2161 ttatgactgt cttctttatc atgcaactcg taggacaggt gccggcagcg ctcttccgct
     2221 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
     2281 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
     2341 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
     2401 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
     2461 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
     2521 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
     2581 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
     2641 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
     2701 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
     2761 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
     2821 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
     2881 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
     2941 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
     3001 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
     3061 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
     3121 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
     3181 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
     3241 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
     3301 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
     3361 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
     3421 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
     3481 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
     3541 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
     3601 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
     3661 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
     3721 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
     3781 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
     3841 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
     3901 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
     3961 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
     4021 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
     4081 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
     4141 cctgacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg
     4201 accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc
     4261 gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga
     4321 tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt
     4381 gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat
     4441 agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat
     4501 ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa
     4561 tttaacgcga attttaacaa aatattaacg cttacaattt gccattcgcc attcaggctg
     4621 cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc tattacgcca gcccaagcta
     4681 ccatgataag taagtaatat taaggtacgg gaggtacttg gagcggccgc aataaaatat
     4741 ctttattttc attacatctg tgtgttggtt ttttgtgtga atcgatagta ctaacatacg
     4801 ctctccatca aaacaaaacg aaacaaaaca aactagcaaa ataggctgtc cccagtgcaa
     4861 gtgcaggtgc cagaacattt ctctatcgat aggtaccgag ctcttacgcg tgctagctca
     4921 gaccgttaga cgccaggacg gg
//
