LOCUS       62056_11670        5097 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5097)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..5097
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..92
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from
                     the human alpha-2 globin gene"
     promoter        234..430
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     CDS             466..2115
                     /codon_start=1
                     /label=luciferase
                     /note="firefly luciferase"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     polyA_signal    complement(2159..2280)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(2699..3287)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3461..4318)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(4319..4423)
                     /label=AmpR promoter
     rep_origin      4450..4905
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     polyA_signal    5036..5084
                     /label=poly(A) signal
                     /note="synthetic polyadenylation signal"
ORIGIN
        1 aacatacgct ctccatcaaa acaaaacgaa acaaaacaaa ctagcaaaat aggctgtccc
       61 cagtgcaagt gcaggtgcca gaacatttct ctatcgatag gtaccgagct cggtgaactt
      121 tcggtgaacc ctaccctcga gtgaactttc ggtgaaccct accctcgagg gtagggttca
      181 ccgaaagttc actcgacgaa ttccagtcta gcccgggctc gagatctgcg atctgcatct
      241 caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc
      301 cagttccgcc cattctccgc cccatcgctg actaattttt tttatttatg cagaggccga
      361 ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg
      421 cttttgcaaa aagcttggca ttccggtact gttggtaaag ccaccatgga agacgccaaa
      481 aacataaaga aaggcccggc gccattctat ccgctggaag atggaaccgc tggagagcaa
      541 ctgcataagg ctatgaagag atacgccctg gttcctggaa caattgcttt tacagatgca
      601 catatcgagg tggacatcac ttacgctgag tacttcgaaa tgtccgttcg gttggcagaa
      661 gctatgaaac gatatgggct gaatacaaat cacagaatcg tcgtatgcag tgaaaactct
      721 cttcaattct ttatgccggt gttgggcgcg ttatttatcg gagttgcagt tgcgcccgcg
      781 aacgacattt ataatgaacg tgaattgctc aacagtatgg gcatttcgca gcctaccgtg
      841 gtgttcgttt ccaaaaaggg gttgcaaaaa attttgaacg tgcaaaaaaa gctcccaatc
      901 atccaaaaaa ttattatcat ggattctaaa acggattacc agggatttca gtcgatgtac
      961 acgttcgtca catctcatct acctcccggt tttaatgaat acgattttgt gccagagtcc
     1021 ttcgataggg acaagacaat tgcactgatc atgaactcct ctggatctac tggtctgcct
     1081 aaaggtgtcg ctctgcctca tagaactgcc tgcgtgagat tctcgcatgc cagagatcct
     1141 atttttggca atcaaatcat tccggatact gcgattttaa gtgttgttcc attccatcac
     1201 ggttttggaa tgtttactac actcggatat ttgatatgtg gatttcgagt cgtcttaatg
     1261 tatagatttg aagaagagct gtttctgagg agccttcagg attacaagat tcaaagtgcg
     1321 ctgctggtgc caaccctatt ctccttcttc gccaaaagca ctctgattga caaatacgat
     1381 ttatctaatt tacacgaaat tgcttctggt ggcgctcccc tctctaagga agtcggggaa
     1441 gcggttgcca agaggttcca tctgccaggt atcaggcaag gatatgggct cactgagact
     1501 acatcagcta ttctgattac acccgagggg gatgataaac cgggcgcggt cggtaaagtt
     1561 gttccatttt ttgaagcgaa ggttgtggat ctggataccg ggaaaacgct gggcgttaat
     1621 caaagaggcg aactgtgtgt gagaggtcct atgattatgt ccggttatgt aaacaatccg
     1681 gaagcgacca acgccttgat tgacaaggat ggatggctac attctggaga catagcttac
     1741 tgggacgaag acgaacactt cttcatcgtt gaccgcctga agtctctgat taagtacaaa
     1801 ggctatcagg tggctcccgc tgaattggaa tccatcttgc tccaacaccc caacatcttc
     1861 gacgcaggtg tcgcaggtct tcccgacgat gacgccggtg aacttcccgc cgccgttgtt
     1921 gttttggagc acggaaagac gatgacggaa aaagagatcg tggattacgt cgccagtcaa
     1981 gtaacaaccg cgaaaaagtt gcgcggagga gttgtgtttg tggacgaagt accgaaaggt
     2041 cttaccggaa aactcgacgc aagaaaaatc agagagatcc tcataaaggc caagaagggc
     2101 ggaaagatcg ccgtgtaatt ctagagtcgg ggcggccggc cgcttcgagc agacatgata
     2161 agatacattg atgagtttgg acaaaccaca actagaatgc agtgaaaaaa atgctttatt
     2221 tgtgaaattt gtgatgctat tgctttattt gtaaccatta taagctgcaa taaacaagtt
     2281 aacaacaaca attgcattca ttttatgttt caggttcagg gggaggtgtg ggaggttttt
     2341 taaagcaagt aaaacctcta caaatgtggt aaaatcgata aggatccgtc gaccgatgcc
     2401 cttgagagcc ttcaacccag tcagctcctt ccggtgggcg cggggcatga ctatcgtcgc
     2461 cgcacttatg actgtcttct ttatcatgca actcgtagga caggtgccgg cagcgctctt
     2521 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag
     2581 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca
     2641 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
     2701 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
     2761 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
     2821 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
     2881 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
     2941 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
     3001 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
     3061 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
     3121 actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct
     3181 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
     3241 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
     3301 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
     3361 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
     3421 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg
     3481 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt
     3541 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag
     3601 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc
     3661 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag
     3721 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca
     3781 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa
     3841 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga
     3901 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata
     3961 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca
     4021 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg
     4081 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg
     4141 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg
     4201 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag
     4261 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac
     4321 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca
     4381 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag
     4441 tgccacctga cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca
     4501 gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct
     4561 ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt
     4621 tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac
     4681 gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct
     4741 ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt
     4801 ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac
     4861 aaaaatttaa cgcgaatttt aacaaaatat taacgcttac aatttgccat tcgccattca
     4921 ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta cgccagccca
     4981 agctaccatg ataagtaagt aatattaagg tacgggaggt acttggagcg gccgcaataa
     5041 aatatcttta ttttcattac atctgtgtgt tggttttttg tgtgaatcga tagtact
//
