LOCUS       Exported                5010 bp DNA     circular SYN 10-SEP-2025
DEFINITION  SV40 promoter-containing vector for measuring the activity of 
            enhancer sequences with a luciferase assay.
ACCESSION   U47298
VERSION     .
KEYWORDS    pGL3-Promoter
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5010)
  AUTHORS   Promega
  TITLE     Direct Submission
  JOURNAL   Exported Sep 10, 2025 from SnapGene 6.0.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5010
                     /lab_host="Mammalian Cells"
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    100..148
                     /note="synthetic polyadenylation signal"
     misc_feature    162..253
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     202..221
                     /label=RVprimer3
     misc_feature    261..301
                     /label=MCS
                     /note="multiple cloning site"
     promoter        308..504
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      355..490
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             540..2192
                     /codon_start=1
                     /gene="luc+"
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="enhanced luc+ version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     primer_bind     complement(541..563)
                     /label=GLprimer2
     polyA_signal    2233..2354
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2513..2532)
                     /label=RVprimer4
     rep_origin      complement(2773..3361)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number colE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3532..4392)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(4393..4497)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      4524..4979
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
ORIGIN
        1 ggaagggcga tcggtgcggg cctcttcgct attacgccag cccaagctac catgataagt
       61 aagtaatatt aaggtacggg aggtacttgg agcggccgca ataaaatatc tttattttca
      121 ttacatctgt gtgttggttt tttgtgtgaa tcgatagtac taacatacgc tctccatcaa
      181 aacaaaacga aacaaaacaa actagcaaaa taggctgtcc ccagtgcaag tgcaggtgcc
      241 agaacatttc tctatcgata ggtaccgagc tcttacgcgt gctagcccgg gctcgagatc
      301 tgcgatctgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg
      361 cccctaactc cgcccagttc cgcccattct ccgccccatc gctgactaat tttttttatt
      421 tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt
      481 tttggaggcc taggcttttg caaaaagctt ggcattccgg tactgttggt aaagccacca
      541 tggaagacgc caaaaacata aagaaaggcc cggcgccatt ctatccgctg gaagatggaa
      601 ccgctggaga gcaactgcat aaggctatga agagatacgc cctggttcct ggaacaattg
      661 cttttacaga tgcacatatc gaggtggaca tcacttacgc tgagtacttc gaaatgtccg
      721 ttcggttggc agaagctatg aaacgatatg ggctgaatac aaatcacaga atcgtcgtat
      781 gcagtgaaaa ctctcttcaa ttctttatgc cggtgttggg cgcgttattt atcggagttg
      841 cagttgcgcc cgcgaacgac atttataatg aacgtgaatt gctcaacagt atgggcattt
      901 cgcagcctac cgtggtgttc gtttccaaaa aggggttgca aaaaattttg aacgtgcaaa
      961 aaaagctccc aatcatccaa aaaattatta tcatggattc taaaacggat taccagggat
     1021 ttcagtcgat gtacacgttc gtcacatctc atctacctcc cggttttaat gaatacgatt
     1081 ttgtgccaga gtccttcgat agggacaaga caattgcact gatcatgaac tcctctggat
     1141 ctactggtct gcctaaaggt gtcgctctgc ctcatagaac tgcctgcgtg agattctcgc
     1201 atgccagaga tcctattttt ggcaatcaaa tcattccgga tactgcgatt ttaagtgttg
     1261 ttccattcca tcacggtttt ggaatgttta ctacactcgg atatttgata tgtggatttc
     1321 gagtcgtctt aatgtataga tttgaagaag agctgtttct gaggagcctt caggattaca
     1381 agattcaaag tgcgctgctg gtgccaaccc tattctcctt cttcgccaaa agcactctga
     1441 ttgacaaata cgatttatct aatttacacg aaattgcttc tggtggcgct cccctctcta
     1501 aggaagtcgg ggaagcggtt gccaagaggt tccatctgcc aggtatcagg caaggatatg
     1561 ggctcactga gactacatca gctattctga ttacacccga gggggatgat aaaccgggcg
     1621 cggtcggtaa agttgttcca ttttttgaag cgaaggttgt ggatctggat accgggaaaa
     1681 cgctgggcgt taatcaaaga ggcgaactgt gtgtgagagg tcctatgatt atgtccggtt
     1741 atgtaaacaa tccggaagcg accaacgcct tgattgacaa ggatggatgg ctacattctg
     1801 gagacatagc ttactgggac gaagacgaac acttcttcat cgttgaccgc ctgaagtctc
     1861 tgattaagta caaaggctat caggtggctc ccgctgaatt ggaatccatc ttgctccaac
     1921 accccaacat cttcgacgca ggtgtcgcag gtcttcccga cgatgacgcc ggtgaacttc
     1981 ccgccgccgt tgttgttttg gagcacggaa agacgatgac ggaaaaagag atcgtggatt
     2041 acgtcgccag tcaagtaaca accgcgaaaa agttgcgcgg aggagttgtg tttgtggacg
     2101 aagtaccgaa aggtcttacc ggaaaactcg acgcaagaaa aatcagagag atcctcataa
     2161 aggccaagaa gggcggaaag atcgccgtgt aattctagag tcggggcggc cggccgcttc
     2221 gagcagacat gataagatac attgatgagt ttggacaaac cacaactaga atgcagtgaa
     2281 aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt atttgtaacc attataagct
     2341 gcaataaaca agttaacaac aacaattgca ttcattttat gtttcaggtt cagggggagg
     2401 tgtgggaggt tttttaaagc aagtaaaacc tctacaaatg tggtaaaatc gataaggatc
     2461 cgtcgaccga tgcccttgag agccttcaac ccagtcagct ccttccggtg ggcgcggggc
     2521 atgactatcg tcgccgcact tatgactgtc ttctttatca tgcaactcgt aggacaggtg
     2581 ccggcagcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
     2641 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
     2701 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
     2761 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
     2821 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     2881 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     2941 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
     3001 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     3061 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
     3121 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
     3181 gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct
     3241 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     3301 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
     3361 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
     3421 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
     3481 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
     3541 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
     3601 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
     3661 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc
     3721 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
     3781 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
     3841 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
     3901 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
     3961 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
     4021 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
     4081 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
     4141 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
     4201 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
     4261 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
     4321 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
     4381 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
     4441 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
     4501 atttccccga aaagtgccac ctgacgcgcc ctgtagcggc gcattaagcg cggcgggtgt
     4561 ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc
     4621 tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg
     4681 gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta
     4741 gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt
     4801 ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat
     4861 ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa
     4921 tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa atattaacgc ttacaatttg
     4981 ccattcgcca ttcaggctgc gcaactgttg
//
