LOCUS       62056_11665        6228 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6228)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..6228
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1498..3147
                     /codon_start=1
                     /label=luciferase
                     /note="firefly luciferase"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     polyA_signal    complement(3191..3312)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(3731..4319)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4493..5350)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(5351..5455)
                     /label=AmpR promoter
     rep_origin      5482..5937
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     polyA_signal    6068..6116
                     /label=poly(A) signal
                     /note="synthetic polyadenylation signal"
     misc_feature    6130..6221
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from
                     the human alpha-2 globin gene"
ORIGIN
        1 ggtaccaaga tcagtgatga ttgtgtcatt aatcaaagat tataattcat ccatctgttc
       61 tcctgaagtc tgcctcctca gaaaggtaag gctaattttc taatccgggg cagccacagg
      121 agaaactgaa atgagtggga ggggtggtaa ctgttttcac ttttgtcttc ctgttttttt
      181 tttttttttt ttcagagcct gggaaagcaa gtcagaattt ccaatctttt ccaattccca
      241 catgattccc taatttagaa cgaacacaga gaaaataaag tctgccttcc tctgggtaag
      301 actccgaagc ctgctgggga ttgcccccac cctcggacaa ggaacccccc gcggaggggg
      361 ttgctccagt cccaccctca ccccgcacga acgcttcttg ggcttggcct tggggacaat
      421 attctggcga cgggtcacag gatggttaat tagtggaaat gctttgtgcg agctggacca
      481 gcaggaaacc ctggggacac aggcggggtg agcctgccga ttcaaacccc tggagggtcc
      541 cggacctgct aaaacacaaa cgtcaggctg cagggtcgag gctccagaga tctacccctc
      601 ggtgccttct gccacccatt gccccggagg gatgcctctt aaaccgagga gcctgggctc
      661 ggttcgccag cgggagcgga cgaggcacgg agcctcggtc cacactagcg tgcacccatc
      721 gtccgagcac tgccaggtga agtccgggcg ctttcctttc ccctctctgc ctccagactg
      781 gggggacaag aaatttaaaa aatgcttccg cggccccaac caccacgaag caccttcatc
      841 gattaggaag ccgtgtggag tttagccacc cggtgtttgc ggtcgaagca ggcccgagcc
      901 tttcccagcg cagggcaggc agccgaagcc cagcagctct ggccctgttc cagaccgggc
      961 ccgaggtgac atcaccactc ctccgatcgg cgggaggggg cccccgaaat cccctaaaaa
     1021 cattgtgagt aatcgcccgg cccgccctcc aggcggggcc cagactcgcg agcgatccgt
     1081 gctgcccagc cagtcccggg agcggcgtcc aggcgcgcgg cgtctcgggg cccagcgcgg
     1141 cgcgtacttg ctcgcagggc ccggcagcgg cggcgcgcgc caccccctag gccctcgagt
     1201 ggcgcgctct tgtccctaac ccaccgcgcg gggcggcggc ggcggcggcg gcggcgacgc
     1261 agcgcgcagg cgcggccgga ttcccggcag tgacgcggcg gcgggcgcgc gcggcgcatt
     1321 tccgcctctg gcgaatggct cgactgtagc gcgcgccgcg ggcccagctg cgaccccggc
     1381 cccgcccccg ggaccccggc catggacgac ctcttccccc tcatcttccc ctcggagccg
     1441 gccccggcct cgggccccta tcaagcttgg cattccggta ctgttggtaa agccaccatg
     1501 gaagacgcca aaaacataaa gaaaggcccg gcgccattct atccgctgga agatggaacc
     1561 gctggagagc aactgcataa ggctatgaag agatacgccc tggttcctgg aacaattgct
     1621 tttacagatg cacatatcga ggtggacatc acttacgctg agtacttcga aatgtccgtt
     1681 cggttggcag aagctatgaa acgatatggg ctgaatacaa atcacagaat cgtcgtatgc
     1741 agtgaaaact ctcttcaatt ctttatgccg gtgttgggcg cgttatttat cggagttgca
     1801 gttgcgcccg cgaacgacat ttataatgaa cgtgaattgc tcaacagtat gggcatttcg
     1861 cagcctaccg tggtgttcgt ttccaaaaag gggttgcaaa aaattttgaa cgtgcaaaaa
     1921 aagctcccaa tcatccaaaa aattattatc atggattcta aaacggatta ccagggattt
     1981 cagtcgatgt acacgttcgt cacatctcat ctacctcccg gttttaatga atacgatttt
     2041 gtgccagagt ccttcgatag ggacaagaca attgcactga tcatgaactc ctctggatct
     2101 actggtctgc ctaaaggtgt cgctctgcct catagaactg cctgcgtgag attctcgcat
     2161 gccagagatc ctatttttgg caatcaaatc attccggata ctgcgatttt aagtgttgtt
     2221 ccattccatc acggttttgg aatgtttact acactcggat atttgatatg tggatttcga
     2281 gtcgtcttaa tgtatagatt tgaagaagag ctgtttctga ggagccttca ggattacaag
     2341 attcaaagtg cgctgctggt gccaacccta ttctccttct tcgccaaaag cactctgatt
     2401 gacaaatacg atttatctaa tttacacgaa attgcttctg gtggcgctcc cctctctaag
     2461 gaagtcgggg aagcggttgc caagaggttc catctgccag gtatcaggca aggatatggg
     2521 ctcactgaga ctacatcagc tattctgatt acacccgagg gggatgataa accgggcgcg
     2581 gtcggtaaag ttgttccatt ttttgaagcg aaggttgtgg atctggatac cgggaaaacg
     2641 ctgggcgtta atcaaagagg cgaactgtgt gtgagaggtc ctatgattat gtccggttat
     2701 gtaaacaatc cggaagcgac caacgccttg attgacaagg atggatggct acattctgga
     2761 gacatagctt actgggacga agacgaacac ttcttcatcg ttgaccgcct gaagtctctg
     2821 attaagtaca aaggctatca ggtggctccc gctgaattgg aatccatctt gctccaacac
     2881 cccaacatct tcgacgcagg tgtcgcaggt cttcccgacg atgacgccgg tgaacttccc
     2941 gccgccgttg ttgttttgga gcacggaaag acgatgacgg aaaaagagat cgtggattac
     3001 gtcgccagtc aagtaacaac cgcgaaaaag ttgcgcggag gagttgtgtt tgtggacgaa
     3061 gtaccgaaag gtcttaccgg aaaactcgac gcaagaaaaa tcagagagat cctcataaag
     3121 gccaagaagg gcggaaagat cgccgtgtaa ttctagagtc ggggcggccg gccgcttcga
     3181 gcagacatga taagatacat tgatgagttt ggacaaacca caactagaat gcagtgaaaa
     3241 aaatgcttta tttgtgaaat ttgtgatgct attgctttat ttgtaaccat tataagctgc
     3301 aataaacaag ttaacaacaa caattgcatt cattttatgt ttcaggttca gggggaggtg
     3361 tgggaggttt tttaaagcaa gtaaaacctc tacaaatgtg gtaaaatcga taaggatccg
     3421 tcgaccgatg cccttgagag ccttcaaccc agtcagctcc ttccggtggg cgcggggcat
     3481 gactatcgtc gccgcactta tgactgtctt ctttatcatg caactcgtag gacaggtgcc
     3541 ggcagcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
     3601 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
     3661 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
     3721 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
     3781 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
     3841 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
     3901 cttcgggaag cgtggcgctt tctcaatgct cacgctgtag gtatctcagt tcggtgtagg
     3961 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
     4021 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
     4081 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
     4141 agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga
     4201 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
     4261 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
     4321 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
     4381 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
     4441 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
     4501 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
     4561 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa
     4621 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg
     4681 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
     4741 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
     4801 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
     4861 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct
     4921 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
     4981 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
     5041 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
     5101 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
     5161 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
     5221 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
     5281 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
     5341 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
     5401 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
     5461 ttccccgaaa agtgccacct gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg
     5521 tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt
     5581 tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc
     5641 tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg
     5701 gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg
     5761 agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct
     5821 cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg
     5881 agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgttt acaatttccc
     5941 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
     6001 tacgccagcc caagctacca tgataagtaa gtaatattaa ggtacgggag gtacttggag
     6061 cggccgcaat aaaatatctt tattttcatt acatctgtgt gttggttttt tgtgtgaatc
     6121 gatagtacta acatacgctc tccatcaaaa caaaacgaaa caaaacaaac tagcaaaata
     6181 ggctgtcccc agtgcaagtg caggtgccag aacatttctc tatcgata
//
