LOCUS       40924_21973        8596 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Reporter vector pGL3-MSTN-3.8kb, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8596)
  AUTHORS   Bi Y, Qiao X, Hua Z, Zhang L, Liu X, Li L, Hua W, Xiao H, Zhou J, 
            Wei Q, Zheng X.
  TITLE     An asymmetric PCR-based, reliable and rapid single-tube native DNA 
            engineering strategy
  JOURNAL   BMC Biotechnol. 12, 39 (2012)
  PUBMED    22768962
REFERENCE   2  (bases 1 to 8596)
  AUTHORS   Bi Y, Zheng X, Qiao X, Zhang R, Jiang L, Ouyang H, Shao C, Hua Z, 
            Zhou J, Liu X, Li L, Xiao H, Hua W, Zhang L, Wei Q.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2011) Biotechnology Group, Institute of Animal 
            Science and Veterinary, Hubei Academy of Agricultural Science, No. 
            1, Nanhu Yaoyuan, Hongshan District, Wuhan, Hubei 430064, P. R. 
            China
REFERENCE   3  (bases 1 to 8596)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 8596)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Biotechnol. 12, 39 (2012)"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (03-AUG-2011) Biotechnology Group, Institute of Animal Science and 
            Veterinary, Hubei Academy of Agricultural Science, No. 1, Nanhu 
            Yaoyuan, Hongshan District, Wuhan, Hubei 430064, P. R. China"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8596
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      61..3838
                     /label=porcine MSTN promoter
                     /note="porcine MSTN promoter"
                     /regulatory_class="promoter"
     regulatory      3707..3711
                     /regulatory_class="CAAT_signal"
     regulatory      3748..3754
                     /regulatory_class="TATA_box"
     regulatory      3772..3778
                     /regulatory_class="TATA_box"
     CDS             3866..5515
                     /label=luciferase
                     /note="firefly luciferase"
     polyA_signal    complement(5559..5680)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(6099..6687)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(6861..7718)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(7719..7823)
                     /label=AmpR promoter
     rep_origin      7850..8305
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     polyA_signal    8436..8484
                     /label=poly(A) signal
                     /note="synthetic polyadenylation signal"
     misc_feature    8498..8589
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from
                     the human alpha-2 globin gene"
ORIGIN
        1 ggtaccgagc tcttacgcgt gctagcccgg gctcgagatc tgcgatctaa gtaagcttgg
       61 catcattaaa cttctgacaa gcctttatgt taatgcactt agtaattccc agactgtaga
      121 gtaaaaccat aaatgttttt ctgggaaata cacttttttt tttggccaca cccatggcat
      181 gtgggagttc ctgggccagg gattgaatcc gcactatagc agtgaccaga gccactgcag
      241 ttgtcaacac tggctatgcc acaagagaac tccctgggaa atatgcttta tattttcaaa
      301 gttgttttga aacacccccc tggcatttgt aagagaagtg agagaagttc cttgctccct
      361 tcggttatag aaatataagg ccctcatcca ctataaagaa cagggggaag gaaaaagata
      421 agtcttgtga aagagctgca ctggccacct aaattgactc atgtgagaaa ctgctttcaa
      481 atacggaaca cctaagacaa agccaacaag ctggtggacc acaccctccc aattaactag
      541 aagggattct ctactcactc attctgatga cacagtccat tcttcatgaa tgcagccaag
      601 gctaggcccc catcagtcac accacagtga tggttcctat ggctggtgaa ccttccttct
      661 ctggggcttt ggaaagattg cagtcactga cactggagaa ggaatatagg ttctcaaaac
      721 ttcttgagta cctattaact cattcaatac tgaccttgag cacagttgat tagactcagt
      781 tgattctcat tattcatagc agttgtgttc tatcaaatca ctgccaacac tgaatgaatg
      841 atggatcact gagccattgc tcctagggga agtacaaagt taggttccta tcagcctctg
      901 gtcacatttt caccaaccaa tcaatatata accttgtttt atgttcattt ctgttttaaa
      961 atatttattt aatatatatt attgatttat taacattgta ttcataggca aacacactat
     1021 aactcaagcc tgaattatgc ttacataata cacatatttt tccattaagg aaattatttt
     1081 ccctaagtca tagcacaacc ttcttttttt taaattgtgg taaaatatac ataagacagt
     1141 tcagtgtaca gttcagtggc attaaataca tttatattgt tgtgcaatca tgatcaatat
     1201 ccatctccag aactttttga ccttcccaaa ttgaaaattt tgtatctatt gaacagtagg
     1261 tttcccccat cccaccccac ctctcctcca gcacctggta accaccattc tcctttccat
     1321 cacagccttc ctgtatgtag gagcacagca ctatgctttg ggaccatttt aaacagcaga
     1381 atcatcccct aaaaaaacaa gtaaaaatgt gaaaaacatg gtactataca gattgcaaag
     1441 agggcacttg cttaaatctg agagctgaaa caaggcagag aattgccttg tttgacctca
     1501 actgggaccc tgtgtcctca agatttttgc tgttttgtac aggcatttga atgactatca
     1561 aagtgccatg agtattgatt ctggagttaa aaataaattt cagcaagtag acaaatttgc
     1621 atacagggaa tccatgaata acgagcatta gctgtacgga aatgacagct ttgtttcctt
     1681 tactccaggt actaagaata aagagctgaa atgtggcttg tgatagggtt gtcttggaat
     1741 atttcacttg cctcagaggc ctagagagct agcctatgtg aactgataat tggcagctac
     1801 aacctgaagc agttctagtt catgtggagg ataaacttaa gcattatctc aaacccctct
     1861 gcatgaaaca aagaccaaac attcaagtac tagttatcaa tcacttacta tgtgacaggc
     1921 actatactca gcaatttaca tgcattattg aattacatcc cccaaaactg aaggttagag
     1981 aagctaagta tctcatccat tattacactg ttagaagtgg caaagttgag atttgaactc
     2041 aggtctatct gactccaaag cctatttccc aacagctttg caattctatt caagtttaaa
     2101 aaaaaaaata tctgatttac tcagaagtgt ataggagcat atgttatgat tattataata
     2161 ttacaaagat ttatatgttg aaaaataaat ttaccaaaaa aaccctttat aagcctgatc
     2221 taatattgct ccacaacaaa gaatttctga aatccttcag ggcatctggt ttgtgtctgt
     2281 ttttccttaa tctttaatga tgagcaagtc taatgcatta tgtaaggcca tttttctcaa
     2341 gagatgtaga tacctcctaa gaatttgatg aaaatgcatt aacttttcag gctactgagt
     2401 tgcattttag tgcactgagg cagtaaatga gtgtacaatg tgcaaaagta gtgacctaaa
     2461 aaataaatat ttgatatgaa ccactgcatt ctcttggaaa aaaaaaaaag taatggatta
     2521 actctcttag gagtccttag cttccccaaa aggagtagga agaataaatc tcctgtggcc
     2581 tggaaacagc ttctgtttct cactggctat gtttgtttag ctctttaata gttcatttga
     2641 ttagatcttg tggctcctaa agctaaggtt gagagtttga gctctacaga ggccacttaa
     2701 atttagagaa caaaaagctc tattctctgc tcccagacct taccccaaat ccctgccagg
     2761 tgtctgccct ctggtcaaat gagaaactgg caaaggggtg caaacctagc acagaattgg
     2821 gaaacagaaa aatgggcacc ctttattatg gtgctccttc tcttttatgt gtttacaata
     2881 cttgggcata atttacagag aatagatact acatttttta ctttcaccac tggaaatctg
     2941 aggcaaactg cattatcagt cataaaattc attatctttc tattctaagt tattctaagc
     3001 ttattctaag ctcagatagc tgacattatc ctcttggtaa taaacaatga aaaaacacat
     3061 cttctgagca atattaatct gcaactttag gataggagaa aatcagttga aaactgagca
     3121 cgattttcac gtgaataaaa gatattattt aaaaataatt ccatgtgtaa tataacagaa
     3181 taagtatgat tttcattatg tactagaaat ttagtcagga aaacaagttt ctcaaattat
     3241 agctgaatat attttactag tatcacaatc ttaaatttta attcaggtct tcctaattta
     3301 aatctgtatt tctctgatta cacaggacta aaaataattt aaaacagcaa ataaaattct
     3361 tttttcctca aatgtttgtc taaataatgt aaaatcattt tatttttttg aggaaaaaga
     3421 catttcaact ttttaagtat gaagtgtaaa agaattactt atttaaatta caattttaaa
     3481 gtttcactaa taaagattaa taatatttaa gtgcagttta tattattgtt aacatagatt
     3541 ttaatttttc aaatgtcaca tatatctttc attatttgta gatttatttc ttttatgaag
     3601 tagtcaaatg aatcagctca cccttgactg taacaaaata ctgtttggtg acttgtgaca
     3661 gacagggttt taacctctga cagcgagatt cattgtggag caagagccaa tcatagatcc
     3721 tgacgacact tgtctcatca agtggaatat aaaaagccac ttggaataca gtataaaaga
     3781 ttcactggtg tggcaagttg tctctcagac agtgcaggca ttaaaatttt gcttggcgca
     3841 ttccggtact gttggtaaag ccaccatgga agacgccaaa aacataaaga aaggcccggc
     3901 gccattctat ccgctggaag atggaaccgc tggagagcaa ctgcataagg ctatgaagag
     3961 atacgccctg gttcctggaa caattgcttt tacagatgca catatcgagg tggacatcac
     4021 ttacgctgag tacttcgaaa tgtccgttcg gttggcagaa gctatgaaac gatatgggct
     4081 gaatacaaat cacagaatcg tcgtatgcag tgaaaactct cttcaattct ttatgccggt
     4141 gttgggcgcg ttatttatcg gagttgcagt tgcgcccgcg aacgacattt ataatgaacg
     4201 tgaattgctc aacagtatgg gcatttcgca gcctaccgtg gtgttcgttt ccaaaaaggg
     4261 gttgcaaaaa attttgaacg tgcaaaaaaa gctcccaatc atccaaaaaa ttattatcat
     4321 ggattctaaa acggattacc agggatttca gtcgatgtac acgttcgtca catctcatct
     4381 acctcccggt tttaatgaat acgattttgt gccagagtcc ttcgataggg acaagacaat
     4441 tgcactgatc atgaactcct ctggatctac tggtctgcct aaaggtgtcg ctctgcctca
     4501 tagaactgcc tgcgtgagat tctcgcatgc cagagatcct atttttggca atcaaatcat
     4561 tccggatact gcgattttaa gtgttgttcc attccatcac ggttttggaa tgtttactac
     4621 actcggatat ttgatatgtg gatttcgagt cgtcttaatg tatagatttg aagaagagct
     4681 gtttctgagg agccttcagg attacaagat tcaaagtgcg ctgctggtgc caaccctatt
     4741 ctccttcttc gccaaaagca ctctgattga caaatacgat ttatctaatt tacacgaaat
     4801 tgcttctggt ggcgctcccc tctctaagga agtcggggaa gcggttgcca agaggttcca
     4861 tctgccaggt atcaggcaag gatatgggct cactgagact acatcagcta ttctgattac
     4921 acccgagggg gatgataaac cgggcgcggt cggtaaagtt gttccatttt ttgaagcgaa
     4981 ggttgtggat ctggataccg ggaaaacgct gggcgttaat caaagaggcg aactgtgtgt
     5041 gagaggtcct atgattatgt ccggttatgt aaacaatccg gaagcgacca acgccttgat
     5101 tgacaaggat ggatggctac attctggaga catagcttac tgggacgaag acgaacactt
     5161 cttcatcgtt gaccgcctga agtctctgat taagtacaaa ggctatcagg tggctcccgc
     5221 tgaattggaa tccatcttgc tccaacaccc caacatcttc gacgcaggtg tcgcaggtct
     5281 tcccgacgat gacgccggtg aacttcccgc cgccgttgtt gttttggagc acggaaagac
     5341 gatgacggaa aaagagatcg tggattacgt cgccagtcaa gtaacaaccg cgaaaaagtt
     5401 gcgcggagga gttgtgtttg tggacgaagt accgaaaggt cttaccggaa aactcgacgc
     5461 aagaaaaatc agagagatcc tcataaaggc caagaagggc ggaaagatcg ccgtgtaatt
     5521 ctagagtcgg ggcggccggc cgcttcgagc agacatgata agatacattg atgagtttgg
     5581 acaaaccaca actagaatgc agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat
     5641 tgctttattt gtaaccatta taagctgcaa taaacaagtt aacaacaaca attgcattca
     5701 ttttatgttt caggttcagg gggaggtgtg ggaggttttt taaagcaagt aaaacctcta
     5761 caaatgtggt aaaatcgata aggatccgtc gaccgatgcc cttgagagcc ttcaacccag
     5821 tcagctcctt ccggtgggcg cggggcatga ctatcgtcgc cgcacttatg actgtcttct
     5881 ttatcatgca actcgtagga caggtgccgg cagcgctctt ccgcttcctc gctcactgac
     5941 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
     6001 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
     6061 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
     6121 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
     6181 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
     6241 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcaatgctca
     6301 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
     6361 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     6421 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
     6481 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg
     6541 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
     6601 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
     6661 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
     6721 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
     6781 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
     6841 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
     6901 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
     6961 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
     7021 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
     7081 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
     7141 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg
     7201 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
     7261 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
     7321 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
     7381 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
     7441 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc
     7501 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
     7561 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
     7621 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
     7681 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
     7741 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
     7801 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga cgcgccctgt
     7861 agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc
     7921 agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc
     7981 tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg
     8041 cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga
     8101 tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc
     8161 caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg
     8221 ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt
     8281 aacaaaatat taacgtttac aatttcccat tcgccattca ggctgcgcaa ctgttgggaa
     8341 gggcgatcgg tgcgggcctc ttcgctatta cgccagccca agctaccatg ataagtaagt
     8401 aatattaagg tacgggaggt acttggagcg gccgcaataa aatatcttta ttttcattac
     8461 atctgtgtgt tggttttttg tgtgaatcga tagtactaac atacgctctc catcaaaaca
     8521 aaacgaaaca aaacaaacta gcaaaatagg ctgtccccag tgcaagtgca ggtgccagaa
     8581 catttctcta tcgata
//
