LOCUS       40924_21968        7094 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Reporter vector pGL3-MSTN-2.3kb, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7094)
  AUTHORS   Bi Y, Qiao X, Hua Z, Zhang L, Liu X, Li L, Hua W, Xiao H, Zhou J, 
            Wei Q, Zheng X.
  TITLE     An asymmetric PCR-based, reliable and rapid single-tube native DNA 
            engineering strategy
  JOURNAL   BMC Biotechnol. 12, 39 (2012)
  PUBMED    22768962
REFERENCE   2  (bases 1 to 7094)
  AUTHORS   Bi Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2011) Biotechnology Group, Institute of Animal 
            Science and Veterinary, Hubei Academy of Agricultural Science, No. 
            1, Nanhu Yaoyuan, Hongshan District, Wuhan, Hubei 430064, P. R. 
            China
REFERENCE   3  (bases 1 to 7094)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7094)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Biotechnol. 12, 39 (2012)"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (03-AUG-2011) Biotechnology Group, Institute of Animal Science and 
            Veterinary, Hubei Academy of Agricultural Science, No. 1, Nanhu 
            Yaoyuan, Hongshan District, Wuhan, Hubei 430064, P. R. China"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7094
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      61..2336
                     /regulatory_class="promoter"
     regulatory      2205..2209
                     /regulatory_class="CAAT_signal"
     regulatory      2246..2252
                     /regulatory_class="TATA_box"
     regulatory      2270..2276
                     /regulatory_class="TATA_box"
     CDS             2364..4013
                     /label=luciferase
                     /note="firefly luciferase"
     polyA_signal    complement(4057..4178)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(4597..5185)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(5359..6216)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(6217..6321)
                     /label=AmpR promoter
     rep_origin      6348..6803
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     polyA_signal    6934..6982
                     /label=poly(A) signal
                     /note="synthetic polyadenylation signal"
     misc_feature    6996..7087
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from
                     the human alpha-2 globin gene"
ORIGIN
        1 ggtaccgagc tcttacgcgt gctagcccgg gctcgagatc tgcgatctaa gtaagcttgg
       61 gtgccatgag tattgattct ggagttaaaa ataaatttca gcaagtagac aaatttgcat
      121 acagggaatc catgaataac gagcattagc tgtacggaaa tgacagcttt gtttccttta
      181 ctccaggtac taagaataaa gagctgaaat gtggcttgtg atagggttgt cttggaatat
      241 ttcacttgcc tcagaggcct agagagctag cctatgtgaa ctgataattg gcagctacaa
      301 cctgaagcag ttctagttca tgtggaggat aaacttaagc attatctcaa acccctctgc
      361 atgaaacaaa gaccaaacat tcaagtacta gttatcaatc acttactatg tgacaggcac
      421 tatactcagc aatttacatg cattattgaa ttacatcccc caaaactgaa ggttagagaa
      481 gctaagtatc tcatccatta ttacactgtt agaagtggca aagttgagat ttgaactcag
      541 gtctatctga ctccaaagcc tatttcccaa cagctttgca attctattca agtttaaaaa
      601 aaaaaatatc tgatttactc agaagtgtat aggagcatat gttatgatta ttataatatt
      661 acaaagattt atatgttgaa aaataaattt accaaaaaaa ccctttataa gcctgatcta
      721 atattgctcc acaacaaaga atttctgaaa tccttcaggg catctggttt gtgtctgttt
      781 ttccttaatc tttaatgatg agcaagtcta atgcattatg taaggccatt tttctcaaga
      841 gatgtagata cctcctaaga atttgatgaa aatgcattaa cttttcaggc tactgagttg
      901 cattttagtg cactgaggca gtaaatgagt gtacaatgtg caaaagtagt gacctaaaaa
      961 ataaatattt gatatgaacc actgcattct cttggaaaaa aaaaaaagta atggattaac
     1021 tctcttagga gtccttagct tccccaaaag gagtaggaag aataaatctc ctgtggcctg
     1081 gaaacagctt ctgtttctca ctggctatgt ttgtttagct ctttaatagt tcatttgatt
     1141 agatcttgtg gctcctaaag ctaaggttga gagtttgagc tctacagagg ccacttaaat
     1201 ttagagaaca aaaagctcta ttctctgctc ccagacctta ccccaaatcc ctgccaggtg
     1261 tctgccctct ggtcaaatga gaaactggca aaggggtgca aacctagcac agaattggga
     1321 aacagaaaaa tgggcaccct ttattatggt gctccttctc ttttatgtgt ttacaatact
     1381 tgggcataat ttacagagaa tagatactac attttttact ttcaccactg gaaatctgag
     1441 gcaaactgca ttatcagtca taaaattcat tatctttcta ttctaagtta ttctaagctt
     1501 attctaagct cagatagctg acattatcct cttggtaata aacaatgaaa aaacacatct
     1561 tctgagcaat attaatctgc aactttagga taggagaaaa tcagttgaaa actgagcacg
     1621 attttcacgt gaataaaaga tattatttaa aaataattcc atgtgtaata taacagaata
     1681 agtatgattt tcattatgta ctagaaattt agtcaggaaa acaagtttct caaattatag
     1741 ctgaatatat tttactagta tcacaatctt aaattttaat tcaggtcttc ctaatttaaa
     1801 tctgtatttc tctgattaca caggactaaa aataatttaa aacagcaaat aaaattcttt
     1861 tttcctcaaa tgtttgtcta aataatgtaa aatcatttta tttttttgag gaaaaagaca
     1921 tttcaacttt ttaagtatga agtgtaaaag aattacttat ttaaattaca attttaaagt
     1981 ttcactaata aagattaata atatttaagt gcagtttata ttattgttaa catagatttt
     2041 aatttttcaa atgtcacata tatctttcat tatttgtaga tttatttctt ttatgaagta
     2101 gtcaaatgaa tcagctcacc cttgactgta acaaaatact gtttggtgac ttgtgacaga
     2161 cagggtttta acctctgaca gcgagattca ttgtggagca agagccaatc atagatcctg
     2221 acgacacttg tctcatcaag tggaatataa aaagccactt ggaatacagt ataaaagatt
     2281 cactggtgtg gcaagttgtc tctcagacag tgcaggcatt aaaattttgc ttggcgcatt
     2341 ccggtactgt tggtaaagcc accatggaag acgccaaaaa cataaagaaa ggcccggcgc
     2401 cattctatcc gctggaagat ggaaccgctg gagagcaact gcataaggct atgaagagat
     2461 acgccctggt tcctggaaca attgctttta cagatgcaca tatcgaggtg gacatcactt
     2521 acgctgagta cttcgaaatg tccgttcggt tggcagaagc tatgaaacga tatgggctga
     2581 atacaaatca cagaatcgtc gtatgcagtg aaaactctct tcaattcttt atgccggtgt
     2641 tgggcgcgtt atttatcgga gttgcagttg cgcccgcgaa cgacatttat aatgaacgtg
     2701 aattgctcaa cagtatgggc atttcgcagc ctaccgtggt gttcgtttcc aaaaaggggt
     2761 tgcaaaaaat tttgaacgtg caaaaaaagc tcccaatcat ccaaaaaatt attatcatgg
     2821 attctaaaac ggattaccag ggatttcagt cgatgtacac gttcgtcaca tctcatctac
     2881 ctcccggttt taatgaatac gattttgtgc cagagtcctt cgatagggac aagacaattg
     2941 cactgatcat gaactcctct ggatctactg gtctgcctaa aggtgtcgct ctgcctcata
     3001 gaactgcctg cgtgagattc tcgcatgcca gagatcctat ttttggcaat caaatcattc
     3061 cggatactgc gattttaagt gttgttccat tccatcacgg ttttggaatg tttactacac
     3121 tcggatattt gatatgtgga tttcgagtcg tcttaatgta tagatttgaa gaagagctgt
     3181 ttctgaggag ccttcaggat tacaagattc aaagtgcgct gctggtgcca accctattct
     3241 ccttcttcgc caaaagcact ctgattgaca aatacgattt atctaattta cacgaaattg
     3301 cttctggtgg cgctcccctc tctaaggaag tcggggaagc ggttgccaag aggttccatc
     3361 tgccaggtat caggcaagga tatgggctca ctgagactac atcagctatt ctgattacac
     3421 ccgaggggga tgataaaccg ggcgcggtcg gtaaagttgt tccatttttt gaagcgaagg
     3481 ttgtggatct ggataccggg aaaacgctgg gcgttaatca aagaggcgaa ctgtgtgtga
     3541 gaggtcctat gattatgtcc ggttatgtaa acaatccgga agcgaccaac gccttgattg
     3601 acaaggatgg atggctacat tctggagaca tagcttactg ggacgaagac gaacacttct
     3661 tcatcgttga ccgcctgaag tctctgatta agtacaaagg ctatcaggtg gctcccgctg
     3721 aattggaatc catcttgctc caacacccca acatcttcga cgcaggtgtc gcaggtcttc
     3781 ccgacgatga cgccggtgaa cttcccgccg ccgttgttgt tttggagcac ggaaagacga
     3841 tgacggaaaa agagatcgtg gattacgtcg ccagtcaagt aacaaccgcg aaaaagttgc
     3901 gcggaggagt tgtgtttgtg gacgaagtac cgaaaggtct taccggaaaa ctcgacgcaa
     3961 gaaaaatcag agagatcctc ataaaggcca agaagggcgg aaagatcgcc gtgtaattct
     4021 agagtcgggg cggccggccg cttcgagcag acatgataag atacattgat gagtttggac
     4081 aaaccacaac tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt gatgctattg
     4141 ctttatttgt aaccattata agctgcaata aacaagttaa caacaacaat tgcattcatt
     4201 ttatgtttca ggttcagggg gaggtgtggg aggtttttta aagcaagtaa aacctctaca
     4261 aatgtggtaa aatcgataag gatccgtcga ccgatgccct tgagagcctt caacccagtc
     4321 agctccttcc ggtgggcgcg gggcatgact atcgtcgccg cacttatgac tgtcttcttt
     4381 atcatgcaac tcgtaggaca ggtgccggca gcgctcttcc gcttcctcgc tcactgactc
     4441 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
     4501 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
     4561 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
     4621 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
     4681 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
     4741 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc aatgctcacg
     4801 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
     4861 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
     4921 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
     4981 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac
     5041 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
     5101 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
     5161 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
     5221 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
     5281 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
     5341 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
     5401 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
     5461 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
     5521 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
     5581 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
     5641 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt
     5701 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
     5761 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
     5821 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
     5881 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
     5941 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
     6001 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
     6061 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
     6121 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
     6181 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
     6241 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
     6301 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg cgccctgtag
     6361 cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag
     6421 cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt
     6481 tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca
     6541 cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata
     6601 gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca
     6661 aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc
     6721 gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa
     6781 caaaatatta acgtttacaa tttcccattc gccattcagg ctgcgcaact gttgggaagg
     6841 gcgatcggtg cgggcctctt cgctattacg ccagcccaag ctaccatgat aagtaagtaa
     6901 tattaaggta cgggaggtac ttggagcggc cgcaataaaa tatctttatt ttcattacat
     6961 ctgtgtgttg gttttttgtg tgaatcgata gtactaacat acgctctcca tcaaaacaaa
     7021 acgaaacaaa acaaactagc aaaataggct gtccccagtg caagtgcagg tgccagaaca
     7081 tttctctatc gata
//
