LOCUS       62056_11650        6833 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6833)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..6833
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             2103..3752
                     /codon_start=1
                     /label=luciferase
                     /note="firefly luciferase"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     polyA_signal    complement(3796..3917)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(4336..4924)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(5098..5955)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(5956..6060)
                     /label=AmpR promoter
     rep_origin      6087..6542
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     polyA_signal    6673..6721
                     /label=poly(A) signal
                     /note="synthetic polyadenylation signal"
     misc_feature    6735..6826
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from
                     the human alpha-2 globin gene"
ORIGIN
        1 ggtaccaagg aaggcagcta ggaccttcag agtgtcctcc cggagcgcca gctcttcaga
       61 gcctgccatg gaggcaccta taagagaagg agctggggat ggactccagg gttcagcaag
      121 aactgccggc tgggggccca aattgctagt cccggaatga gaagtgtggt gccgtcagga
      181 ttcatgcgtg cccaagggag gaaggggctg agagtctgga ttcccaggtt ccttacagaa
      241 gttggggtct gggggcccag acacttgagt tctcagaagg aggagaggga tgagggcaaa
      301 gacatcctga tcctgggaga ggagaaagct ggggatttgg atttggggtc caagggagga
      361 ggggactggg ggcctggact cttggttttc ggagtagggg gctgggggtc tgaactcttg
      421 agtctaagag agaaggagac tggggcctca gatccctgag tccccgaagg agaaggaggc
      481 ttggggctct gacttctcca ttctgaaagt atatgccaag ctattcaagt ccgtagagga
      541 tcaggaacta cgattcccag cagacttcgt agcaaacatt cccctccgtc cgttacttgt
      601 tcgccgcgca gcctgctggg aggtgtagtt ccactctgac tcgggctgga atttggggga
      661 gccgggacac gcagatgtcg gggaagcgtg actgggctac ctgctcctag atcccggccc
      721 ccttttcttc cctgctcacc tagctctccc tcacctcccg ctcacctggt cccgggcccc
      781 gccgccgccg cctcctccac tgaacttgta taaactttcc ttagggcgac tttcccgcgc
      841 ctgcgcaatg actgtaccgg ctaggagccg gcccaaacgt ggaaggggcg gggcgatgag
      901 gcccgcctaa tttgcataga acgctcccag catgcccgtg gtcggaagac ctaaaaaggg
      961 catgatgggc gggggagttt tcccggctca ctgtaccgcc tctctggctg tcgagtcgat
     1021 aatcctaacg acgttaaagt cgtttttatt tcaagaaatc aacagaaaac aagtcccacc
     1081 tctaaaactc agctgacgag aagtggggtg tgcggcggcg agttcgagag ctacggggct
     1141 tcaaacgcag gagtgggttt tctgggtttc tgggttcctg ggttcccggc gcacctcctc
     1201 ccggcactct acgcgcctcg acccactacc taggtaacgc ggaaaagcgg gggagttggt
     1261 ggcgtccagt agtgaagaat gggacggagg aaggatgcgt ctgccttgac agtggggaag
     1321 ggggtttgtg tacgctgggg aaagtgaagc gagaaggaaa ggaagtgaag caatggctgg
     1381 ctgtaaaaca aaacttagaa aagtagttgg taggagagaa gaggaggtgt ccacactggg
     1441 gtatctaggg gagagaagga ctctgtgaat gagaagcgat ccactctggg aagtggggcg
     1501 tgccgcggag gagggaggcc gtctgtttgg gaggaactgt ggttggtcgg tctatgagtg
     1561 aggggttctg gggagcgggg ggagctacac tggagttgag tgtgggaggt agaggtctgc
     1621 gagtccctag catgtgcacg gcagtaacgg gagggaggcg tctaccctgg agataagagt
     1681 acctgcactg tgtgatgtgg gggagggaag ggctttcagt ggagtgggga aggtctacac
     1741 tggcggaatt gaggaaggta gaattgaggg aggtagaggt atgtagataa aagaagctgg
     1801 ggaaaggaga ggggttttgg agggactcct ctctgaatta gggagcctct ctggaggatg
     1861 ggaggtctcc tgtctgtacc ccaggagaat agaggtgagg accctgcgcc gtggcgcttg
     1921 agagggaggt ctgggttcgg aaccttccaa ctgggtgggc ctgggagtcc gcctatatcc
     1981 aggaactgtc cccggcgcac aggcccatgg gaactcagaa gcctcggatc ctgccctggc
     2041 tgatatctca gctgaaccag gggcaacaag cttggcattc cggtactgtt ggtaaagcca
     2101 ccatggaaga cgccaaaaac ataaagaaag gcccggcgcc attctatccg ctggaagatg
     2161 gaaccgctgg agagcaactg cataaggcta tgaagagata cgccctggtt cctggaacaa
     2221 ttgcttttac agatgcacat atcgaggtgg acatcactta cgctgagtac ttcgaaatgt
     2281 ccgttcggtt ggcagaagct atgaaacgat atgggctgaa tacaaatcac agaatcgtcg
     2341 tatgcagtga aaactctctt caattcttta tgccggtgtt gggcgcgtta tttatcggag
     2401 ttgcagttgc gcccgcgaac gacatttata atgaacgtga attgctcaac agtatgggca
     2461 tttcgcagcc taccgtggtg ttcgtttcca aaaaggggtt gcaaaaaatt ttgaacgtgc
     2521 aaaaaaagct cccaatcatc caaaaaatta ttatcatgga ttctaaaacg gattaccagg
     2581 gatttcagtc gatgtacacg ttcgtcacat ctcatctacc tcccggtttt aatgaatacg
     2641 attttgtgcc agagtccttc gatagggaca agacaattgc actgatcatg aactcctctg
     2701 gatctactgg tctgcctaaa ggtgtcgctc tgcctcatag aactgcctgc gtgagattct
     2761 cgcatgccag agatcctatt tttggcaatc aaatcattcc ggatactgcg attttaagtg
     2821 ttgttccatt ccatcacggt tttggaatgt ttactacact cggatatttg atatgtggat
     2881 ttcgagtcgt cttaatgtat agatttgaag aagagctgtt tctgaggagc cttcaggatt
     2941 acaagattca aagtgcgctg ctggtgccaa ccctattctc cttcttcgcc aaaagcactc
     3001 tgattgacaa atacgattta tctaatttac acgaaattgc ttctggtggc gctcccctct
     3061 ctaaggaagt cggggaagcg gttgccaaga ggttccatct gccaggtatc aggcaaggat
     3121 atgggctcac tgagactaca tcagctattc tgattacacc cgagggggat gataaaccgg
     3181 gcgcggtcgg taaagttgtt ccattttttg aagcgaaggt tgtggatctg gataccggga
     3241 aaacgctggg cgttaatcaa agaggcgaac tgtgtgtgag aggtcctatg attatgtccg
     3301 gttatgtaaa caatccggaa gcgaccaacg ccttgattga caaggatgga tggctacatt
     3361 ctggagacat agcttactgg gacgaagacg aacacttctt catcgttgac cgcctgaagt
     3421 ctctgattaa gtacaaaggc tatcaggtgg ctcccgctga attggaatcc atcttgctcc
     3481 aacaccccaa catcttcgac gcaggtgtcg caggtcttcc cgacgatgac gccggtgaac
     3541 ttcccgccgc cgttgttgtt ttggagcacg gaaagacgat gacggaaaaa gagatcgtgg
     3601 attacgtcgc cagtcaagta acaaccgcga aaaagttgcg cggaggagtt gtgtttgtgg
     3661 acgaagtacc gaaaggtctt accggaaaac tcgacgcaag aaaaatcaga gagatcctca
     3721 taaaggccaa gaagggcgga aagatcgccg tgtaattcta gagtcggggc ggccggccgc
     3781 ttcgagcaga catgataaga tacattgatg agtttggaca aaccacaact agaatgcagt
     3841 gaaaaaaatg ctttatttgt gaaatttgtg atgctattgc tttatttgta accattataa
     3901 gctgcaataa acaagttaac aacaacaatt gcattcattt tatgtttcag gttcaggggg
     3961 aggtgtggga ggttttttaa agcaagtaaa acctctacaa atgtggtaaa atcgataagg
     4021 atccgtcgac cgatgccctt gagagccttc aacccagtca gctccttccg gtgggcgcgg
     4081 ggcatgacta tcgtcgccgc acttatgact gtcttcttta tcatgcaact cgtaggacag
     4141 gtgccggcag cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
     4201 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
     4261 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
     4321 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
     4381 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
     4441 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
     4501 tctcccttcg ggaagcgtgg cgctttctca atgctcacgc tgtaggtatc tcagttcggt
     4561 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
     4621 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
     4681 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
     4741 cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct
     4801 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
     4861 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
     4921 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
     4981 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
     5041 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca
     5101 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
     5161 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
     5221 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc
     5281 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
     5341 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
     5401 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc
     5461 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag
     5521 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt
     5581 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac
     5641 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg
     5701 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat
     5761 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc
     5821 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
     5881 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
     5941 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg
     6001 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg
     6061 cacatttccc cgaaaagtgc cacctgacgc gccctgtagc ggcgcattaa gcgcggcggg
     6121 tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt
     6181 cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg
     6241 ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga
     6301 ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac
     6361 gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc
     6421 tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct attggttaaa
     6481 aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa cgtttacaat
     6541 ttcccattcg ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc
     6601 gctattacgc cagcccaagc taccatgata agtaagtaat attaaggtac gggaggtact
     6661 tggagcggcc gcaataaaat atctttattt tcattacatc tgtgtgttgg ttttttgtgt
     6721 gaatcgatag tactaacata cgctctccat caaaacaaaa cgaaacaaaa caaactagca
     6781 aaataggctg tccccagtgc aagtgcaggt gccagaacat ttctctatcg ata
//
