LOCUS       62056_11645        6992 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6992)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..6992
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             2262..3911
                     /codon_start=1
                     /label=luciferase
                     /note="firefly luciferase"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     polyA_signal    complement(3955..4076)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(4495..5083)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(5257..6114)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(6115..6219)
                     /label=AmpR promoter
     rep_origin      6246..6701
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     polyA_signal    6832..6880
                     /label=poly(A) signal
                     /note="synthetic polyadenylation signal"
     misc_feature    6894..6985
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from
                     the human alpha-2 globin gene"
ORIGIN
        1 ggtaccgcaa tctcagttca agtgtattgc acagagaagt ggccttgggg actgtgcaga
       61 actggttcca tccagacaca atgcttccac ccaatctcca cagcccacct gagcttattc
      121 ctgacaagag aagcaaccag ctttcctttc tgtagccaga tcattcgcaa caccccagat
      181 ggctccaacc tcctgccctg tggggctgag cctcaccatt tccattgcag ggcacttcaa
      241 atgtgaaata tttacccggg tcgtttctta caggtttccc atggctgcct tggaaaggga
      301 attgtttgtt tgtttgtttg tttgtttcat ttgttttgtt tgttgtttgt ttgtgttgat
      361 gactcggttg agtgccctcc atccagcctt ccacttccct ccacatttgc ttcctctttg
      421 ctctgttttc taagccaaat ccaggaggat gctctctctt cattaaacca acggtctgta
      481 taatgtgagg catctttact gggatgccca gcagaaagaa tgcactggga atgtagggga
      541 gactgaggtc aaggctaaag gaagccaaaa gatctggata actccatcga gctgaggtgg
      601 tgctgggtcc tggttgtctg agtcacgacc acaccataga agccagagaa gctgtctctc
      661 atggggggag gggggaggaa gggattcaag taactagaaa aatcaaatca tctttcctcc
      721 aaccttccac aggtgtacag gcagcagcaa ctgcagacat gcaaaccacc tgacctccac
      781 ccatgcccgt tcctcagaac cttcagaaat ggcggacctc tacttggctt ggaatgagtg
      841 ttcgtgaata accaacacca cttttgaaaa cagtgttcct tcttaagaaa gagcagaaaa
      901 ttaatacggc aaatattcat tccaagcaca tggtgtctgt aggcctgggg cagccttagg
      961 aaattcgggg tgctctctgg cttctgaagt tcttggcttg gtttcctggg agtgccgtga
     1021 ttctggtctg gaagcaggca cagcttatcc aatttttaat tccctttcaa gatgattccc
     1081 atggcagtca tttctctgaa attaagatat ttgttagccc caccttcaaa cggatgacct
     1141 taaagaagaa ttccaccagt attgaccttg tgtgctccca agaatctggg ttgcattcac
     1201 ccagaaaaga gggggagggt gactccttat ccttttctat caaaatgctg aggtaaggat
     1261 gtctcaggaa catctaccct attgggtttt ggcaggctgc tgcatatctg cattctgtga
     1321 ttggtctgca aggtgtgtgt gtgtgtgtgt gtgtgtgtgt gtgtgtgtgc atctatgacc
     1381 ttgcaaagtg ccctgagaca agagccagaa agctttggta ctggaaaata cacaatgctg
     1441 ctttcagcct gtggtgtgga aatcagattt cagagggaat aacatcttgc accgtggacc
     1501 tccacgccac tgggtgtggc cacagttatt gtttccttag tggatgttgc atctctttag
     1561 aaaatgtgcc caagaatgaa gattccgaaa acgatgtctg cagaggagga tagaaaacgg
     1621 tcaaattgta cctgggcaga tgcccagata caattctcct ttcattttac cgcattctag
     1681 cagtgaaatt ttgaggatac tggagtcgaa ccagtcagcc tgccttgcct cacccagctc
     1741 ggccccgccc ccaaaggccc cgccctactc acactaggcc ccacccccac ccgccctaag
     1801 ccccgccctc acccgctgcc tccttcacgg cccagaaccg cagcgagcgc cgggtgcggc
     1861 gctgccgccg cagggatgtg accttcaccg tcgcttagcc aggatgaccg gagcccgtgt
     1921 ctcgcggcgt ccgcgcctcg cttcagcctc ccgggtgctc tgaccgcacg ctcccggctg
     1981 ctaggctccc cggcaccggc ctcgccatgc cgccaccgcc cgggcccgcc gccgccctgg
     2041 gcactgcgct tctgctgctc ctgctggctt ccgagtcttc tcacagtgag taccgcaggg
     2101 gtcccccaaa cccactaccc cgtggtgtgg ggctccaggc ctgtaccccg gcgcagcatc
     2161 ctggtgccgg gtaccccaga caccctggca tcctgtcccc ggacctctga gctctaggca
     2221 tcacggaagc ttggcattcc ggtactgttg gtaaagccac catggaagac gccaaaaaca
     2281 taaagaaagg cccggcgcca ttctatccgc tggaagatgg aaccgctgga gagcaactgc
     2341 ataaggctat gaagagatac gccctggttc ctggaacaat tgcttttaca gatgcacata
     2401 tcgaggtgga catcacttac gctgagtact tcgaaatgtc cgttcggttg gcagaagcta
     2461 tgaaacgata tgggctgaat acaaatcaca gaatcgtcgt atgcagtgaa aactctcttc
     2521 aattctttat gccggtgttg ggcgcgttat ttatcggagt tgcagttgcg cccgcgaacg
     2581 acatttataa tgaacgtgaa ttgctcaaca gtatgggcat ttcgcagcct accgtggtgt
     2641 tcgtttccaa aaaggggttg caaaaaattt tgaacgtgca aaaaaagctc ccaatcatcc
     2701 aaaaaattat tatcatggat tctaaaacgg attaccaggg atttcagtcg atgtacacgt
     2761 tcgtcacatc tcatctacct cccggtttta atgaatacga ttttgtgcca gagtccttcg
     2821 atagggacaa gacaattgca ctgatcatga actcctctgg atctactggt ctgcctaaag
     2881 gtgtcgctct gcctcataga actgcctgcg tgagattctc gcatgccaga gatcctattt
     2941 ttggcaatca aatcattccg gatactgcga ttttaagtgt tgttccattc catcacggtt
     3001 ttggaatgtt tactacactc ggatatttga tatgtggatt tcgagtcgtc ttaatgtata
     3061 gatttgaaga agagctgttt ctgaggagcc ttcaggatta caagattcaa agtgcgctgc
     3121 tggtgccaac cctattctcc ttcttcgcca aaagcactct gattgacaaa tacgatttat
     3181 ctaatttaca cgaaattgct tctggtggcg ctcccctctc taaggaagtc ggggaagcgg
     3241 ttgccaagag gttccatctg ccaggtatca ggcaaggata tgggctcact gagactacat
     3301 cagctattct gattacaccc gagggggatg ataaaccggg cgcggtcggt aaagttgttc
     3361 cattttttga agcgaaggtt gtggatctgg ataccgggaa aacgctgggc gttaatcaaa
     3421 gaggcgaact gtgtgtgaga ggtcctatga ttatgtccgg ttatgtaaac aatccggaag
     3481 cgaccaacgc cttgattgac aaggatggat ggctacattc tggagacata gcttactggg
     3541 acgaagacga acacttcttc atcgttgacc gcctgaagtc tctgattaag tacaaaggct
     3601 atcaggtggc tcccgctgaa ttggaatcca tcttgctcca acaccccaac atcttcgacg
     3661 caggtgtcgc aggtcttccc gacgatgacg ccggtgaact tcccgccgcc gttgttgttt
     3721 tggagcacgg aaagacgatg acggaaaaag agatcgtgga ttacgtcgcc agtcaagtaa
     3781 caaccgcgaa aaagttgcgc ggaggagttg tgtttgtgga cgaagtaccg aaaggtctta
     3841 ccggaaaact cgacgcaaga aaaatcagag agatcctcat aaaggccaag aagggcggaa
     3901 agatcgccgt gtaattctag agtcggggcg gccggccgct tcgagcagac atgataagat
     3961 acattgatga gtttggacaa accacaacta gaatgcagtg aaaaaaatgc tttatttgtg
     4021 aaatttgtga tgctattgct ttatttgtaa ccattataag ctgcaataaa caagttaaca
     4081 acaacaattg cattcatttt atgtttcagg ttcaggggga ggtgtgggag gttttttaaa
     4141 gcaagtaaaa cctctacaaa tgtggtaaaa tcgataagga tccgtcgacc gatgcccttg
     4201 agagccttca acccagtcag ctccttccgg tgggcgcggg gcatgactat cgtcgccgca
     4261 cttatgactg tcttctttat catgcaactc gtaggacagg tgccggcagc gctcttccgc
     4321 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
     4381 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
     4441 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
     4501 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
     4561 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
     4621 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
     4681 gctttctcaa tgctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
     4741 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
     4801 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
     4861 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
     4921 cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
     4981 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
     5041 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
     5101 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
     5161 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
     5221 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
     5281 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
     5341 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
     5401 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
     5461 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
     5521 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
     5581 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
     5641 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
     5701 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
     5761 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
     5821 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
     5881 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
     5941 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
     6001 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
     6061 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
     6121 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
     6181 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
     6241 acctgacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt
     6301 gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct
     6361 cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg
     6421 atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag
     6481 tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa
     6541 tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga
     6601 tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa
     6661 atttaacgcg aattttaaca aaatattaac gtttacaatt tcccattcgc cattcaggct
     6721 gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agcccaagct
     6781 accatgataa gtaagtaata ttaaggtacg ggaggtactt ggagcggccg caataaaata
     6841 tctttatttt cattacatct gtgtgttggt tttttgtgtg aatcgatagt actaacatac
     6901 gctctccatc aaaacaaaac gaaacaaaac aaactagcaa aataggctgt ccccagtgca
     6961 agtgcaggtg ccagaacatt tctctatcga ta
//
