LOCUS       62056_11640        4832 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4832)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..4832
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             30..1679
                     /codon_start=1
                     /label=luciferase
                     /note="firefly luciferase"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     polyA_signal    complement(1723..1844)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(2263..2851)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3025..3882)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(3883..3987)
                     /label=AmpR promoter
     rep_origin      4014..4469
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     polyA_signal    4600..4648
                     /label=poly(A) signal
                     /note="synthetic polyadenylation signal"
     misc_feature    4662..4753
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from
                     the human alpha-2 globin gene"
ORIGIN
        1 ggcattccgg tactgttggt aaagccacca tggaagacgc caaaaacata aagaaaggcc
       61 cggcgccatt ctatccgctg gaagatggaa ccgctggaga gcaactgcat aaggctatga
      121 agagatacgc cctggttcct ggaacaattg cttttacaga tgcacatatc gaggtggaca
      181 tcacttacgc tgagtacttc gaaatgtccg ttcggttggc agaagctatg aaacgatatg
      241 ggctgaatac aaatcacaga atcgtcgtat gcagtgaaaa ctctcttcaa ttctttatgc
      301 cggtgttggg cgcgttattt atcggagttg cagttgcgcc cgcgaacgac atttataatg
      361 aacgtgaatt gctcaacagt atgggcattt cgcagcctac cgtggtgttc gtttccaaaa
      421 aggggttgca aaaaattttg aacgtgcaaa aaaagctccc aatcatccaa aaaattatta
      481 tcatggattc taaaacggat taccagggat ttcagtcgat gtacacgttc gtcacatctc
      541 atctacctcc cggttttaat gaatacgatt ttgtgccaga gtccttcgat agggacaaga
      601 caattgcact gatcatgaac tcctctggat ctactggtct gcctaaaggt gtcgctctgc
      661 ctcatagaac tgcctgcgtg agattctcgc atgccagaga tcctattttt ggcaatcaaa
      721 tcattccgga tactgcgatt ttaagtgttg ttccattcca tcacggtttt ggaatgttta
      781 ctacactcgg atatttgata tgtggatttc gagtcgtctt aatgtataga tttgaagaag
      841 agctgtttct gaggagcctt caggattaca agattcaaag tgcgctgctg gtgccaaccc
      901 tattctcctt cttcgccaaa agcactctga ttgacaaata cgatttatct aatttacacg
      961 aaattgcttc tggtggcgct cccctctcta aggaagtcgg ggaagcggtt gccaagaggt
     1021 tccatctgcc aggtatcagg caaggatatg ggctcactga gactacatca gctattctga
     1081 ttacacccga gggggatgat aaaccgggcg cggtcggtaa agttgttcca ttttttgaag
     1141 cgaaggttgt ggatctggat accgggaaaa cgctgggcgt taatcaaaga ggcgaactgt
     1201 gtgtgagagg tcctatgatt atgtccggtt atgtaaacaa tccggaagcg accaacgcct
     1261 tgattgacaa ggatggatgg ctacattctg gagacatagc ttactgggac gaagacgaac
     1321 acttcttcat cgttgaccgc ctgaagtctc tgattaagta caaaggctat caggtggctc
     1381 ccgctgaatt ggaatccatc ttgctccaac accccaacat cttcgacgca ggtgtcgcag
     1441 gtcttcccga cgatgacgcc ggtgaacttc ccgccgccgt tgttgttttg gagcacggaa
     1501 agacgatgac ggaaaaagag atcgtggatt acgtcgccag tcaagtaaca accgcgaaaa
     1561 agttgcgcgg aggagttgtg tttgtggacg aagtaccgaa aggtcttacc ggaaaactcg
     1621 acgcaagaaa aatcagagag atcctcataa aggccaagaa gggcggaaag atcgccgtgt
     1681 aattctagag tcggggcggc cggccgcttc gagcagacat gataagatac attgatgagt
     1741 ttggacaaac cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg
     1801 ctattgcttt atttgtaacc attataagct gcaataaaca agttaacaac aacaattgca
     1861 ttcattttat gtttcaggtt cagggggagg tgtgggaggt tttttaaagc aagtaaaacc
     1921 tctacaaatg tggtaaaatc gataaggatc cgtcgaccga tgcccttgag agccttcaac
     1981 ccagtcagct ccttccggtg ggcgcggggc atgactatcg tcgccgcact tatgactgtc
     2041 ttctttatca tgcaactcgt aggacaggtg ccggcagcgc tcttccgctt cctcgctcac
     2101 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
     2161 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
     2221 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
     2281 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     2341 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     2401 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcaatg
     2461 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     2521 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     2581 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
     2641 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
     2701 aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
     2761 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
     2821 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
     2881 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
     2941 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
     3001 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
     3061 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
     3121 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
     3181 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
     3241 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
     3301 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc
     3361 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
     3421 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
     3481 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
     3541 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
     3601 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca
     3661 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
     3721 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
     3781 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
     3841 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
     3901 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
     3961 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgcgcc
     4021 ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact
     4081 tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc
     4141 cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt
     4201 acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc
     4261 ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt
     4321 gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat
     4381 tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa
     4441 ttttaacaaa atattaacgt ttacaatttc ccattcgcca ttcaggctgc gcaactgttg
     4501 ggaagggcga tcggtgcggg cctcttcgct attacgccag cccaagctac catgataagt
     4561 aagtaatatt aaggtacggg aggtacttgg agcggccgca ataaaatatc tttattttca
     4621 ttacatctgt gtgttggttt tttgtgtgaa tcgatagtac taacatacgc tctccatcaa
     4681 aacaaaacga aacaaaacaa actagcaaaa taggctgtcc ccagtgcaag tgcaggtgcc
     4741 agaacatttc tctatcgata ggtacgcaaa caaacttatt ttgaagcaaa acaaacttat
     4801 tttgaagcaa aacaaactta ttttgaaagc tt
//
