LOCUS       62056_11635        5891 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5891)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..5891
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1161..2810
                     /codon_start=1
                     /label=luciferase
                     /note="firefly luciferase"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     polyA_signal    complement(2854..2975)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(3394..3982)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4156..5013)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(5014..5118)
                     /label=AmpR promoter
     rep_origin      5145..5600
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     polyA_signal    5731..5779
                     /label=poly(A) signal
                     /note="synthetic polyadenylation signal"
     misc_feature    5793..5884
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from
                     the human alpha-2 globin gene"
ORIGIN
        1 ggtaccccaa tgcttactcc tctttccctg ccctcggtgg agtgatgaag ttgctcccat
       61 ccacgcgcta ggtgggaccc cgtgggaaat gctggggatc tagcccctct gactccgctg
      121 cgcacacctc cacgttcact acgtagccgt acacaccatc aagctccttc cctggcgcac
      181 cgggctggcc tgcccctcgt gggagccttc agcactcggg ggaccgatag gcacggcttc
      241 cgcggagata cagaccttga atttgtggga gaatcccgcc ctggcgctca ctcggcgggc
      301 ttattaatgc cctctttatg aagtgtccaa accatctgca tttcagcagc aaaagatgct
      361 gccggcggct tccttctgtg tcccaaaccg tgcagacctg agagtcctgg ggttagggaa
      421 gtggttcccc tgtgcctact gctacctcct cgcttgcctt ccagcctcac ccttactcaa
      481 gcttcaatgc gaagctccgt gtctgccatc tcgcctgtct tctgccacca tcgcccccaa
      541 ttttggacag gtgggctgga tgcccactag ttcctatgca ttctctgtgt ctgagggggt
      601 gggtacaggg ctggatcccc aaggtccagc caggttttca gaaccaagaa agagcctcca
      661 catccaaaca cctgcaatat ccccccactc caaatctggg ctcacaggct aacccagaac
      721 agaagacaat ttttgaaccc aagagctgct ggggaaataa aagtatacga ttgctggagt
      781 ttctaatttc tattaagcag tccctctgga agacagagag gacagagacg ctcttgaagt
      841 caactccata tgccccatca ttgattcctg gattcttctc tcctcacccc tccctcccca
      901 cctcctgccc tgtttgtttt agttacgaaa tgctgtgggc acctcggttg tgactgaaaa
      961 gtaaccttga aacacgccgg cctgaatatc agagacaaat ctcagcctcc caaccgtcgg
     1021 ccgctgctag aggggctgct tgcgccaggc gccggccgcc ccactgcggg tccctggcgg
     1081 ccggtgtctg aggagtcgga gagcctcgag atctgcgatc taagtaagct tggcattccg
     1141 gtactgttgg taaagccacc atggaagacg ccaaaaacat aaagaaaggc ccggcgccat
     1201 tctatccgct ggaagatgga accgctggag agcaactgca taaggctatg aagagatacg
     1261 ccctggttcc tggaacaatt gcttttacag atgcacatat cgaggtggac atcacttacg
     1321 ctgagtactt cgaaatgtcc gttcggttgg cagaagctat gaaacgatat gggctgaata
     1381 caaatcacag aatcgtcgta tgcagtgaaa actctcttca attctttatg ccggtgttgg
     1441 gcgcgttatt tatcggagtt gcagttgcgc ccgcgaacga catttataat gaacgtgaat
     1501 tgctcaacag tatgggcatt tcgcagccta ccgtggtgtt cgtttccaaa aaggggttgc
     1561 aaaaaatttt gaacgtgcaa aaaaagctcc caatcatcca aaaaattatt atcatggatt
     1621 ctaaaacgga ttaccaggga tttcagtcga tgtacacgtt cgtcacatct catctacctc
     1681 ccggttttaa tgaatacgat tttgtgccag agtccttcga tagggacaag acaattgcac
     1741 tgatcatgaa ctcctctgga tctactggtc tgcctaaagg tgtcgctctg cctcatagaa
     1801 ctgcctgcgt gagattctcg catgccagag atcctatttt tggcaatcaa atcattccgg
     1861 atactgcgat tttaagtgtt gttccattcc atcacggttt tggaatgttt actacactcg
     1921 gatatttgat atgtggattt cgagtcgtct taatgtatag atttgaagaa gagctgtttc
     1981 tgaggagcct tcaggattac aagattcaaa gtgcgctgct ggtgccaacc ctattctcct
     2041 tcttcgccaa aagcactctg attgacaaat acgatttatc taatttacac gaaattgctt
     2101 ctggtggcgc tcccctctct aaggaagtcg gggaagcggt tgccaagagg ttccatctgc
     2161 caggtatcag gcaaggatat gggctcactg agactacatc agctattctg attacacccg
     2221 agggggatga taaaccgggc gcggtcggta aagttgttcc attttttgaa gcgaaggttg
     2281 tggatctgga taccgggaaa acgctgggcg ttaatcaaag aggcgaactg tgtgtgagag
     2341 gtcctatgat tatgtccggt tatgtaaaca atccggaagc gaccaacgcc ttgattgaca
     2401 aggatggatg gctacattct ggagacatag cttactggga cgaagacgaa cacttcttca
     2461 tcgttgaccg cctgaagtct ctgattaagt acaaaggcta tcaggtggct cccgctgaat
     2521 tggaatccat cttgctccaa caccccaaca tcttcgacgc aggtgtcgca ggtcttcccg
     2581 acgatgacgc cggtgaactt cccgccgccg ttgttgtttt ggagcacgga aagacgatga
     2641 cggaaaaaga gatcgtggat tacgtcgcca gtcaagtaac aaccgcgaaa aagttgcgcg
     2701 gaggagttgt gtttgtggac gaagtaccga aaggtcttac cggaaaactc gacgcaagaa
     2761 aaatcagaga gatcctcata aaggccaaga agggcggaaa gatcgccgtg taattctaga
     2821 gtcggggcgg ccggccgctt cgagcagaca tgataagata cattgatgag tttggacaaa
     2881 ccacaactag aatgcagtga aaaaaatgct ttatttgtga aatttgtgat gctattgctt
     2941 tatttgtaac cattataagc tgcaataaac aagttaacaa caacaattgc attcatttta
     3001 tgtttcaggt tcagggggag gtgtgggagg ttttttaaag caagtaaaac ctctacaaat
     3061 gtggtaaaat cgataaggat ccgtcgaccg atgcccttga gagccttcaa cccagtcagc
     3121 tccttccggt gggcgcgggg catgactatc gtcgccgcac ttatgactgt cttctttatc
     3181 atgcaactcg taggacaggt gccggcagcg ctcttccgct tcctcgctca ctgactcgct
     3241 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
     3301 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
     3361 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
     3421 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
     3481 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
     3541 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcaat gctcacgctg
     3601 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
     3661 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
     3721 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
     3781 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt
     3841 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
     3901 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
     3961 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
     4021 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
     4081 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac
     4141 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt
     4201 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt
     4261 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt
     4321 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc
     4381 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa
     4441 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg
     4501 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt
     4561 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc
     4621 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt
     4681 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg
     4741 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac
     4801 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc
     4861 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt
     4921 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg
     4981 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag
     5041 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa
     5101 acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgcgc cctgtagcgg
     5161 cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc
     5221 cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc
     5281 ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct
     5341 cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac
     5401 ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac
     5461 tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat
     5521 ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa
     5581 aatattaacg tttacaattt cccattcgcc attcaggctg cgcaactgtt gggaagggcg
     5641 atcggtgcgg gcctcttcgc tattacgcca gcccaagcta ccatgataag taagtaatat
     5701 taaggtacgg gaggtacttg gagcggccgc aataaaatat ctttattttc attacatctg
     5761 tgtgttggtt ttttgtgtga atcgatagta ctaacatacg ctctccatca aaacaaaacg
     5821 aaacaaaaca aactagcaaa ataggctgtc cccagtgcaa gtgcaggtgc cagaacattt
     5881 ctctatcgat a
//
