LOCUS       Exported                4818 bp DNA     circular SYN 11-SEP-2025
DEFINITION  Cloning vector pGL3-Basic, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4818)
  AUTHORS   Groskreutz DJ, Schenborn ET.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JAN-1996) D.J. Groskreutz, R
REFERENCE   2  (bases 1 to 4818)
  AUTHORS   Kenefick K.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2001) Technical Writing, Promega Corporation, 2800
            Woods Hollow Road, Madison, WI 53711-5399, USA
REFERENCE   3  (bases 1 to 4818)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4818)
  TITLE     Direct Submission
REFERENCE   5  (bases 1 to 4818)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Sep 11, 2025 from SnapGene 6.0.2
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted
            (26-JAN-1996) D.J. Groskreutz, R"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (05-MAR-2001) Technical Writing, Promega Corporation, 2800 Woods
            Hollow Road, Madison, WI 53711-5399, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     SGRef: number: 4; type: "Journal Article"
COMMENT     On Mar 5, 2001 this sequence version replaced U47295.1.
FEATURES             Location/Qualifiers
     source          1..4818
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     source          join(240..4818,1..239)
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    79..127
                     /label=poly(A) signal
                     /note="synthetic polyadenylation signal"
     misc_feature    141..232
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from
                     the human alpha-2 globin gene"
     misc_feature    240..297
                     /label=multiple cloning site
                     /note="multiple cloning site"
     CDS             327..1976
                     /codon_start=1
                     /label=luciferase
                     /note="firefly luciferase"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     polyA_signal    complement(2020..2141)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2300..2319)
                     /label=RV primer4 sequencing primer binding site
                     /note="RV primer4 sequencing primer binding site"
     rep_origin      2557
                     /label=ColE1-derived plasmid replication origin
                     /note="ColE1-derived plasmid replication origin"
     rep_origin      complement(2560..3148)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3322..4179)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(4180..4284)
                     /label=AmpR promoter
     rep_origin      4311..4766
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
ORIGIN
        1 ctcttcgcta ttacgccagc ccaagctacc atgataagta agtaatatta aggtacggga
       61 ggtacttgga gcggccgcaa taaaatatct ttattttcat tacatctgtg tgttggtttt
      121 ttgtgtgaat cgatagtact aacatacgct ctccatcaaa acaaaacgaa acaaaacaaa
      181 ctagcaaaat aggctgtccc cagtgcaagt gcaggtgcca gaacatttct ctatcgatag
      241 gtaccgagct cttacgcgtg ctagcccggg ctcgagatct gcgatctaag taagcttggc
      301 attccggtac tgttggtaaa gccaccatgg aagacgccaa aaacataaag aaaggcccgg
      361 cgccattcta tccgctggaa gatggaaccg ctggagagca actgcataag gctatgaaga
      421 gatacgccct ggttcctgga acaattgctt ttacagatgc acatatcgag gtggacatca
      481 cttacgctga gtacttcgaa atgtccgttc ggttggcaga agctatgaaa cgatatgggc
      541 tgaatacaaa tcacagaatc gtcgtatgca gtgaaaactc tcttcaattc tttatgccgg
      601 tgttgggcgc gttatttatc ggagttgcag ttgcgcccgc gaacgacatt tataatgaac
      661 gtgaattgct caacagtatg ggcatttcgc agcctaccgt ggtgttcgtt tccaaaaagg
      721 ggttgcaaaa aattttgaac gtgcaaaaaa agctcccaat catccaaaaa attattatca
      781 tggattctaa aacggattac cagggatttc agtcgatgta cacgttcgtc acatctcatc
      841 tacctcccgg ttttaatgaa tacgattttg tgccagagtc cttcgatagg gacaagacaa
      901 ttgcactgat catgaactcc tctggatcta ctggtctgcc taaaggtgtc gctctgcctc
      961 atagaactgc ctgcgtgaga ttctcgcatg ccagagatcc tatttttggc aatcaaatca
     1021 ttccggatac tgcgatttta agtgttgttc cattccatca cggttttgga atgtttacta
     1081 cactcggata tttgatatgt ggatttcgag tcgtcttaat gtatagattt gaagaagagc
     1141 tgtttctgag gagccttcag gattacaaga ttcaaagtgc gctgctggtg ccaaccctat
     1201 tctccttctt cgccaaaagc actctgattg acaaatacga tttatctaat ttacacgaaa
     1261 ttgcttctgg tggcgctccc ctctctaagg aagtcgggga agcggttgcc aagaggttcc
     1321 atctgccagg tatcaggcaa ggatatgggc tcactgagac tacatcagct attctgatta
     1381 cacccgaggg ggatgataaa ccgggcgcgg tcggtaaagt tgttccattt tttgaagcga
     1441 aggttgtgga tctggatacc gggaaaacgc tgggcgttaa tcaaagaggc gaactgtgtg
     1501 tgagaggtcc tatgattatg tccggttatg taaacaatcc ggaagcgacc aacgccttga
     1561 ttgacaagga tggatggcta cattctggag acatagctta ctgggacgaa gacgaacact
     1621 tcttcatcgt tgaccgcctg aagtctctga ttaagtacaa aggctatcag gtggctcccg
     1681 ctgaattgga atccatcttg ctccaacacc ccaacatctt cgacgcaggt gtcgcaggtc
     1741 ttcccgacga tgacgccggt gaacttcccg ccgccgttgt tgttttggag cacggaaaga
     1801 cgatgacgga aaaagagatc gtggattacg tcgccagtca agtaacaacc gcgaaaaagt
     1861 tgcgcggagg agttgtgttt gtggacgaag taccgaaagg tcttaccgga aaactcgacg
     1921 caagaaaaat cagagagatc ctcataaagg ccaagaaggg cggaaagatc gccgtgtaat
     1981 tctagagtcg gggcggccgg ccgcttcgag cagacatgat aagatacatt gatgagtttg
     2041 gacaaaccac aactagaatg cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta
     2101 ttgctttatt tgtaaccatt ataagctgca ataaacaagt taacaacaac aattgcattc
     2161 attttatgtt tcaggttcag ggggaggtgt gggaggtttt ttaaagcaag taaaacctct
     2221 acaaatgtgg taaaatcgat aaggatccgt cgaccgatgc ccttgagagc cttcaaccca
     2281 gtcagctcct tccggtgggc gcggggcatg actatcgtcg ccgcacttat gactgtcttc
     2341 tttatcatgc aactcgtagg acaggtgccg gcagcgctct tccgcttcct cgctcactga
     2401 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
     2461 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
     2521 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
     2581 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
     2641 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
     2701 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
     2761 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
     2821 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
     2881 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
     2941 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
     3001 aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
     3061 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
     3121 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
     3181 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
     3241 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
     3301 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
     3361 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
     3421 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc
     3481 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
     3541 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
     3601 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc
     3661 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
     3721 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
     3781 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
     3841 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
     3901 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag
     3961 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
     4021 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
     4081 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
     4141 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
     4201 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
     4261 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgcgccctg
     4321 tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc
     4381 cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg
     4441 ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg
     4501 gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg
     4561 atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt
     4621 ccaaactgga acaacactca accctatctc ggtctattct tttgatttat aagggatttt
     4681 gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt
     4741 taacaaaata ttaacgctta caatttgcca ttcgccattc aggctgcgca actgttggga
     4801 agggcgatcg gtgcgggc
//
