LOCUS       62056_11610        6872 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6872)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..6872
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             2142..3791
                     /codon_start=1
                     /label=luciferase
                     /note="firefly luciferase"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     polyA_signal    complement(3835..3956)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(4375..4963)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(5137..5994)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(5995..6099)
                     /label=AmpR promoter
     rep_origin      6126..6581
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     polyA_signal    6712..6760
                     /label=poly(A) signal
                     /note="synthetic polyadenylation signal"
     misc_feature    6774..6865
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from
                     the human alpha-2 globin gene"
ORIGIN
        1 ggtacccccg cctcaactgg cctaatcccc actctctgat cctgtcctgc cttttccaga
       61 aattatcttc tggtcccttg agtctcccct cctgtaacat gcatcctcat ctttattctg
      121 ccagcatttt ttttcaatac cacactcaaa tgttactcaa gtcaaaggct tcctatgaga
      181 gcaggggttg tgcctggcgt tctacagcct ttggttcaca cccccttttt tttttagagg
      241 gggtcttgct ctgttgccca ggctggagtg cagtggcgca atctcggctc actgcaacct
      301 ctgcctccca ggttcaagcg attcttctgc cttagcctcc cgagtagctg agactaggtg
      361 tgggccacca ctaacggcta attttttttt ttttttttgt atttttagta gagaaggggt
      421 ttcaccatat tagccacgct ggtctcgaac tcctaacctc gtgatctgcc cgcctcggcc
      481 tcccaaagtc ctaggattac aaacatgaac caccgcgccg gacgcccggt cttttttttt
      541 tttttttttt aagacatttc ggtctccacg ttggtcaggc tggtctcgaa tgcccgacct
      601 caggtgatcc tcctgcctcg gcctcccaaa gtgctgggat tacaggtgtg agccaccgcg
      661 cccggccggt tcacaccctt tatttgtatt tatttttttt tttttgagac agggtctcac
      721 tttgccgtcc agtctcaagt gcagtggcgc catctcgact cactgcaacc tcgacctcct
      781 gggctcaagg gatcttccta cctcagcctc ccaagtacct gggaccacag acgcgagcca
      841 ctgcccggct attttttgta tttttttgta gagacgggat ttcgctatgt tgccgaggtt
      901 ggtctctaac tcctgggctg aacctatcct tccgcctcag cctcccaaag tgctgggacg
      961 acaggcttga accaccgcgc ccggcctggt tcacacactt taatggcact taccacatac
     1021 tgctaggcat tgaggttatt tctgtaacat ctcttctttt ctactagatt ctatgagggc
     1081 aggaactagg tcttattttt ctctgaggac aaggactgtt agacaagtgt atggagacgc
     1141 atctagacca actgcctgac ttcctctttc ctctgggttt acttctccct ccaagaggag
     1201 ggttgtgtgt gtgagcgggg cgactggaaa acatagtgtt caggacgttg tatcttgttc
     1261 caggaactaa gtacacgcct gggtaactaa tgtggagtac ctctccttcc cctgttgcta
     1321 tcgcccctct gtggctgctt ggcaagtagc tcagtatgaa gagctgacgg aattctgcgg
     1381 tgggccaggt ggctctggcg tcgactgttt aaggcgactg ttaatgacta caacacacca
     1441 ttgagtcttg gtcccaaggc gctggtgcac atggtacttc ccgaaatccc tgtagggccg
     1501 ctggagcagg agccccgatc ccacgtttgg gcagctgact cgcctaaggc ctccagctga
     1561 aagcggaatt gcaggggtga gaatgagcag acctaactac cgggcttctc gcacctggcc
     1621 tgcatttatt taggaccacg ataacctcga aggccaggct ggggtgcccg atgggtcgtg
     1681 ggcttgctgc ctaactccct gagatcggcg ggctgaacag agcttcgaag tgtagcgcca
     1741 tcctgagcag aggaaggggc gcccgaaggc cgcgtagcgg gcggcagggc gggaggaagt
     1801 agcagctgct gctccccgcc cacccctctc gcgtgtgcct ttccgctcca ctgttcgccg
     1861 gtgagctagg tggcgcgaga gcggagggga gaaaagacag cggctgcagg agccgaaggc
     1921 cgcggctcga ggtggggcag gggcgggcac gcgagcgtgc gtgcgtgggc gcggtctata
     1981 gcacgccgcg cgcggggcgg atgtccgggc cggctgggcc ggggccgcgg aggagatggc
     2041 ggaaggtgga ggctgccgtg agcgaccgga tgcggagact cagaagtccg agcttggacc
     2101 cttaataagc ttggcattcc ggtactgttg gtaaagccac catggaagac gccaaaaaca
     2161 taaagaaagg cccggcgcca ttctatccgc tggaagatgg aaccgctgga gagcaactgc
     2221 ataaggctat gaagagatac gccctggttc ctggaacaat tgcttttaca gatgcacata
     2281 tcgaggtgga catcacttac gctgagtact tcgaaatgtc cgttcggttg gcagaagcta
     2341 tgaaacgata tgggctgaat acaaatcaca gaatcgtcgt atgcagtgaa aactctcttc
     2401 aattctttat gccggtgttg ggcgcgttat ttatcggagt tgcagttgcg cccgcgaacg
     2461 acatttataa tgaacgtgaa ttgctcaaca gtatgggcat ttcgcagcct accgtggtgt
     2521 tcgtttccaa aaaggggttg caaaaaattt tgaacgtgca aaaaaagctc ccaatcatcc
     2581 aaaaaattat tatcatggat tctaaaacgg attaccaggg atttcagtcg atgtacacgt
     2641 tcgtcacatc tcatctacct cccggtttta atgaatacga ttttgtgcca gagtccttcg
     2701 atagggacaa gacaattgca ctgatcatga actcctctgg atctactggt ctgcctaaag
     2761 gtgtcgctct gcctcataga actgcctgcg tgagattctc gcatgccaga gatcctattt
     2821 ttggcaatca aatcattccg gatactgcga ttttaagtgt tgttccattc catcacggtt
     2881 ttggaatgtt tactacactc ggatatttga tatgtggatt tcgagtcgtc ttaatgtata
     2941 gatttgaaga agagctgttt ctgaggagcc ttcaggatta caagattcaa agtgcgctgc
     3001 tggtgccaac cctattctcc ttcttcgcca aaagcactct gattgacaaa tacgatttat
     3061 ctaatttaca cgaaattgct tctggtggcg ctcccctctc taaggaagtc ggggaagcgg
     3121 ttgccaagag gttccatctg ccaggtatca ggcaaggata tgggctcact gagactacat
     3181 cagctattct gattacaccc gagggggatg ataaaccggg cgcggtcggt aaagttgttc
     3241 cattttttga agcgaaggtt gtggatctgg ataccgggaa aacgctgggc gttaatcaaa
     3301 gaggcgaact gtgtgtgaga ggtcctatga ttatgtccgg ttatgtaaac aatccggaag
     3361 cgaccaacgc cttgattgac aaggatggat ggctacattc tggagacata gcttactggg
     3421 acgaagacga acacttcttc atcgttgacc gcctgaagtc tctgattaag tacaaaggct
     3481 atcaggtggc tcccgctgaa ttggaatcca tcttgctcca acaccccaac atcttcgacg
     3541 caggtgtcgc aggtcttccc gacgatgacg ccggtgaact tcccgccgcc gttgttgttt
     3601 tggagcacgg aaagacgatg acggaaaaag agatcgtgga ttacgtcgcc agtcaagtaa
     3661 caaccgcgaa aaagttgcgc ggaggagttg tgtttgtgga cgaagtaccg aaaggtctta
     3721 ccggaaaact cgacgcaaga aaaatcagag agatcctcat aaaggccaag aagggcggaa
     3781 agatcgccgt gtaattctag agtcggggcg gccggccgct tcgagcagac atgataagat
     3841 acattgatga gtttggacaa accacaacta gaatgcagtg aaaaaaatgc tttatttgtg
     3901 aaatttgtga tgctattgct ttatttgtaa ccattataag ctgcaataaa caagttaaca
     3961 acaacaattg cattcatttt atgtttcagg ttcaggggga ggtgtgggag gttttttaaa
     4021 gcaagtaaaa cctctacaaa tgtggtaaaa tcgataagga tccgtcgacc gatgcccttg
     4081 agagccttca acccagtcag ctccttccgg tgggcgcggg gcatgactat cgtcgccgca
     4141 cttatgactg tcttctttat catgcaactc gtaggacagg tgccggcagc gctcttccgc
     4201 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
     4261 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
     4321 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
     4381 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
     4441 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
     4501 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
     4561 gctttctcaa tgctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
     4621 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
     4681 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
     4741 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
     4801 cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
     4861 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
     4921 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
     4981 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
     5041 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
     5101 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
     5161 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
     5221 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
     5281 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
     5341 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
     5401 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
     5461 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
     5521 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
     5581 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
     5641 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
     5701 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
     5761 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
     5821 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
     5881 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
     5941 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
     6001 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
     6061 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
     6121 acctgacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt
     6181 gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct
     6241 cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg
     6301 atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag
     6361 tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa
     6421 tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga
     6481 tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa
     6541 atttaacgcg aattttaaca aaatattaac gtttacaatt tcccattcgc cattcaggct
     6601 gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agcccaagct
     6661 accatgataa gtaagtaata ttaaggtacg ggaggtactt ggagcggccg caataaaata
     6721 tctttatttt cattacatct gtgtgttggt tttttgtgtg aatcgatagt actaacatac
     6781 gctctccatc aaaacaaaac gaaacaaaac aaactagcaa aataggctgt ccccagtgca
     6841 agtgcaggtg ccagaacatt tctctatcga ta
//
