LOCUS       62056_11600        6284 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6284)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..6284
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1554..3203
                     /codon_start=1
                     /label=luciferase
                     /note="firefly luciferase"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     polyA_signal    complement(3247..3368)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(3787..4375)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4549..5406)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(5407..5511)
                     /label=AmpR promoter
     rep_origin      5538..5993
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     polyA_signal    6124..6172
                     /label=poly(A) signal
                     /note="synthetic polyadenylation signal"
     misc_feature    6186..6277
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from
                     the human alpha-2 globin gene"
ORIGIN
        1 ggtaccgagc tcttacgcgt gctagcaaac cgcgacttct gggcgtgcag gaaaagcacg
       61 ctggaatcga gtgctgcaga catcctggac cgtggttttt acgcgtgagt aacggaggag
      121 cgcaccgacc ggtggaagag agaagtggct aacgcgtgtc caaaaggggg aaaagtcaca
      181 agagcaacta ttagggtcgc gctcctattg gtcagaggaa agatcccgac tttagaagga
      241 ctttcctgct ggctggctcg cagcgtcccc ggagagcaag cgagcgcgcc gcagcgggag
      301 actaggttct ctccgagcgt cctccgcaga ggcgccgcga gaggagcagg gagccgcagc
      361 tcgccgtgtt gtcttcattt cgtgcaataa agaattgtca ttaggtttgc gtatggcatg
      421 tgctattacc ccaccgtaaa actaaaatta gcaaaatgtc aggaatggaa agattgattc
      481 accaagatgc aattatcatt taaaagtgct tgattgaggt actgatgttc agtgatttat
      541 tctgcatacc atatacataa ttaaagtagt gtagtggagt aatttatcaa tctagttgag
      601 actggagggg tgaggaggga gctgctgtat gtttgtttta ttaaaatgct ccgaggtcta
      661 gtcccgcccc ccttttgcaa gagtgaaact gatgatttct ccagctcgcg aggaaagagt
      721 caacggtttg ggattgtggg ggaaaaaaag acggagagaa aaaggcagcg cgaagcaaag
      781 gcaaggccaa aattaaataa agggggaaaa aggaggcaaa agacatttca tcggacgtgc
      841 tgcttagaac cccaaccatt cgtgctccgt cttcccttac cacccccgcc tcccctccca
      901 gtatccttca atcccccccg cccccaccac ccccagtctt ttttacgcga tgtttcaaac
      961 gctgtgagct gttctccttt tcccattcgt cttctgtcac ttccttcctg gacgcagttt
     1021 tctggacgag tctggttact tttaatccga ccggccgctg agagccactt tctcctcctc
     1081 ctcctcctcc tccttctctt cctcctcctt cttcctcctc ctcctcctct tccgagcggc
     1141 ctcggcgcgc gcgaatgcgc ggccccgcgc cccccccctc gcgcgcgctc ccctcgcgcg
     1201 cgcgcacaca cgcacacatc gtctccagct ctctgctcgc tctgctcgca gtcacagaca
     1261 cttgagcaca cgcgtacacc cagacatctt cgggctgcta ttggattgac tttgaaggtt
     1321 ctgtgtgggt cgccgtggct gcatgtttga atcaggtgga gaagcacttc aacgctggac
     1381 gaagtaaaga ttattgttgt tatttttttt ttctctctct ctctctctta agaaaggaaa
     1441 atatcccaag gactaatctg atcgggtctt ccttcatgta agtacccctg atatttcctc
     1501 gagatctgcg atctaagtaa gcttggcatt ccggtactgt tggtaaagcc accatggaag
     1561 acgccaaaaa cataaagaaa ggcccggcgc cattctatcc gctggaagat ggaaccgctg
     1621 gagagcaact gcataaggct atgaagagat acgccctggt tcctggaaca attgctttta
     1681 cagatgcaca tatcgaggtg gacatcactt acgctgagta cttcgaaatg tccgttcggt
     1741 tggcagaagc tatgaaacga tatgggctga atacaaatca cagaatcgtc gtatgcagtg
     1801 aaaactctct tcaattcttt atgccggtgt tgggcgcgtt atttatcgga gttgcagttg
     1861 cgcccgcgaa cgacatttat aatgaacgtg aattgctcaa cagtatgggc atttcgcagc
     1921 ctaccgtggt gttcgtttcc aaaaaggggt tgcaaaaaat tttgaacgtg caaaaaaagc
     1981 tcccaatcat ccaaaaaatt attatcatgg attctaaaac ggattaccag ggatttcagt
     2041 cgatgtacac gttcgtcaca tctcatctac ctcccggttt taatgaatac gattttgtgc
     2101 cagagtcctt cgatagggac aagacaattg cactgatcat gaactcctct ggatctactg
     2161 gtctgcctaa aggtgtcgct ctgcctcata gaactgcctg cgtgagattc tcgcatgcca
     2221 gagatcctat ttttggcaat caaatcattc cggatactgc gattttaagt gttgttccat
     2281 tccatcacgg ttttggaatg tttactacac tcggatattt gatatgtgga tttcgagtcg
     2341 tcttaatgta tagatttgaa gaagagctgt ttctgaggag ccttcaggat tacaagattc
     2401 aaagtgcgct gctggtgcca accctattct ccttcttcgc caaaagcact ctgattgaca
     2461 aatacgattt atctaattta cacgaaattg cttctggtgg cgctcccctc tctaaggaag
     2521 tcggggaagc ggttgccaag aggttccatc tgccaggtat caggcaagga tatgggctca
     2581 ctgagactac atcagctatt ctgattacac ccgaggggga tgataaaccg ggcgcggtcg
     2641 gtaaagttgt tccatttttt gaagcgaagg ttgtggatct ggataccggg aaaacgctgg
     2701 gcgttaatca aagaggcgaa ctgtgtgtga gaggtcctat gattatgtcc ggttatgtaa
     2761 acaatccgga agcgaccaac gccttgattg acaaggatgg atggctacat tctggagaca
     2821 tagcttactg ggacgaagac gaacacttct tcatcgttga ccgcctgaag tctctgatta
     2881 agtacaaagg ctatcaggtg gctcccgctg aattggaatc catcttgctc caacacccca
     2941 acatcttcga cgcaggtgtc gcaggtcttc ccgacgatga cgccggtgaa cttcccgccg
     3001 ccgttgttgt tttggagcac ggaaagacga tgacggaaaa agagatcgtg gattacgtcg
     3061 ccagtcaagt aacaaccgcg aaaaagttgc gcggaggagt tgtgtttgtg gacgaagtac
     3121 cgaaaggtct taccggaaaa ctcgacgcaa gaaaaatcag agagatcctc ataaaggcca
     3181 agaagggcgg aaagatcgcc gtgtaattct agagtcgggg cggccggccg cttcgagcag
     3241 acatgataag atacattgat gagtttggac aaaccacaac tagaatgcag tgaaaaaaat
     3301 gctttatttg tgaaatttgt gatgctattg ctttatttgt aaccattata agctgcaata
     3361 aacaagttaa caacaacaat tgcattcatt ttatgtttca ggttcagggg gaggtgtggg
     3421 aggtttttta aagcaagtaa aacctctaca aatgtggtaa aatcgataag gatccgtcga
     3481 ccgatgccct tgagagcctt caacccagtc agctccttcc ggtgggcgcg gggcatgact
     3541 atcgtcgccg cacttatgac tgtcttcttt atcatgcaac tcgtaggaca ggtgccggca
     3601 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
     3661 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
     3721 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
     3781 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
     3841 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
     3901 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
     3961 gggaagcgtg gcgctttctc aatgctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
     4021 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
     4081 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
     4141 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
     4201 gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc
     4261 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
     4321 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
     4381 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
     4441 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
     4501 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
     4561 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
     4621 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
     4681 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
     4741 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
     4801 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
     4861 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
     4921 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
     4981 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
     5041 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
     5101 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
     5161 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
     5221 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
     5281 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
     5341 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
     5401 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
     5461 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
     5521 ccgaaaagtg ccacctgacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt
     5581 tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt
     5641 cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc
     5701 tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga
     5761 tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc
     5821 cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt
     5881 ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct
     5941 gatttaacaa aaatttaacg cgaattttaa caaaatatta acgtttacaa tttcccattc
     6001 gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg
     6061 ccagcccaag ctaccatgat aagtaagtaa tattaaggta cgggaggtac ttggagcggc
     6121 cgcaataaaa tatctttatt ttcattacat ctgtgtgttg gttttttgtg tgaatcgata
     6181 gtactaacat acgctctcca tcaaaacaaa acgaaacaaa acaaactagc aaaataggct
     6241 gtccccagtg caagtgcagg tgccagaaca tttctctatc gata
//
