LOCUS       62056_11615        5807 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5807)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..5807
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1077..2726
                     /codon_start=1
                     /label=luciferase
                     /note="firefly luciferase"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     polyA_signal    complement(2770..2891)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(3310..3898)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4072..4929)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(4930..5034)
                     /label=AmpR promoter
     rep_origin      5061..5516
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     polyA_signal    5647..5695
                     /label=poly(A) signal
                     /note="synthetic polyadenylation signal"
     misc_feature    5709..5800
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from
                     the human alpha-2 globin gene"
ORIGIN
        1 ggtaccagcc ttgatgggaa tgacttacaa gccgcggtgc gggctgagcc tgcctctctc
       61 ggggttcttt ccccaaggga gtgggagaca attgggcctg gaacctaaaa ggaagagagc
      121 ctgtgctagc cgcggggtag aggggaagga gaagagcttt gggccgaagt gtccgcacac
      181 cctggagcaa gagacacttg tgcactgaaa ttctaaccgt gggatgcaag gagagttgaa
      241 caggggaaca ggtctggctc ttggctctag gtttaagaga tacacactgt gtgtgtgtcc
      301 ctaggtgaat aaaaatccga ctcccgctgg ctcggtgcga cccagctttt cccttacccc
      361 ctccgtttcc ccatcttaaa gccctattgg gaaacgaaag tggacaccac tgcctcccgc
      421 cccccacccc aatccaagtc ttccaagtgt ctgtggccga taagagcacc gggaccgccc
      481 ccctgccggt cttctctgcc tgggagacag atggggggcg gggcccatcc gagagagggc
      541 ggaggaggcc ccgggtgagg aagaagcggg ggggtgagaa ctgagagaat ctgaatcggg
      601 aggcgaaggg gacggggagg agggctagga ggactccgag cccgggggag ggggagggag
      661 tagctctgcg ccaatcagtg gcgccggcct gggaggttgc tagcggtatc cacgtaaatc
      721 aaagggcgcg gagccaatgg gagggggcgg agggggcggg gcccaggcgc gtgccgctgc
      781 gagccggcgc tgccaagaga gcgggagaga gctggagaga gcagggagag gggggagcgc
      841 cgagctagtc agagagtgag cgagagcgag aaggagggag aggaggagaa agagagcgag
      901 ggcgggcggg aggcggcggc ggcggcagca gcagcagtaa tagcaggagc agcaacagaa
      961 ggcgtcggag cgggcgtcgg agctgcccgc tgtgggagag agaggagaca gaaagagcga
     1021 ctcgagatct gcgatctaag taagcttggc attccggtac tgttggtaaa gccaccatgg
     1081 aagacgccaa aaacataaag aaaggcccgg cgccattcta tccgctggaa gatggaaccg
     1141 ctggagagca actgcataag gctatgaaga gatacgccct ggttcctgga acaattgctt
     1201 ttacagatgc acatatcgag gtggacatca cttacgctga gtacttcgaa atgtccgttc
     1261 ggttggcaga agctatgaaa cgatatgggc tgaatacaaa tcacagaatc gtcgtatgca
     1321 gtgaaaactc tcttcaattc tttatgccgg tgttgggcgc gttatttatc ggagttgcag
     1381 ttgcgcccgc gaacgacatt tataatgaac gtgaattgct caacagtatg ggcatttcgc
     1441 agcctaccgt ggtgttcgtt tccaaaaagg ggttgcaaaa aattttgaac gtgcaaaaaa
     1501 agctcccaat catccaaaaa attattatca tggattctaa aacggattac cagggatttc
     1561 agtcgatgta cacgttcgtc acatctcatc tacctcccgg ttttaatgaa tacgattttg
     1621 tgccagagtc cttcgatagg gacaagacaa ttgcactgat catgaactcc tctggatcta
     1681 ctggtctgcc taaaggtgtc gctctgcctc atagaactgc ctgcgtgaga ttctcgcatg
     1741 ccagagatcc tatttttggc aatcaaatca ttccggatac tgcgatttta agtgttgttc
     1801 cattccatca cggttttgga atgtttacta cactcggata tttgatatgt ggatttcgag
     1861 tcgtcttaat gtatagattt gaagaagagc tgtttctgag gagccttcag gattacaaga
     1921 ttcaaagtgc gctgctggtg ccaaccctat tctccttctt cgccaaaagc actctgattg
     1981 acaaatacga tttatctaat ttacacgaaa ttgcttctgg tggcgctccc ctctctaagg
     2041 aagtcgggga agcggttgcc aagaggttcc atctgccagg tatcaggcaa ggatatgggc
     2101 tcactgagac tacatcagct attctgatta cacccgaggg ggatgataaa ccgggcgcgg
     2161 tcggtaaagt tgttccattt tttgaagcga aggttgtgga tctggatacc gggaaaacgc
     2221 tgggcgttaa tcaaagaggc gaactgtgtg tgagaggtcc tatgattatg tccggttatg
     2281 taaacaatcc ggaagcgacc aacgccttga ttgacaagga tggatggcta cattctggag
     2341 acatagctta ctgggacgaa gacgaacact tcttcatcgt tgaccgcctg aagtctctga
     2401 ttaagtacaa aggctatcag gtggctcccg ctgaattgga atccatcttg ctccaacacc
     2461 ccaacatctt cgacgcaggt gtcgcaggtc ttcccgacga tgacgccggt gaacttcccg
     2521 ccgccgttgt tgttttggag cacggaaaga cgatgacgga aaaagagatc gtggattacg
     2581 tcgccagtca agtaacaacc gcgaaaaagt tgcgcggagg agttgtgttt gtggacgaag
     2641 taccgaaagg tcttaccgga aaactcgacg caagaaaaat cagagagatc ctcataaagg
     2701 ccaagaaggg cggaaagatc gccgtgtaat tctagagtcg gggcggccgg ccgcttcgag
     2761 cagacatgat aagatacatt gatgagtttg gacaaaccac aactagaatg cagtgaaaaa
     2821 aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt ataagctgca
     2881 ataaacaagt taacaacaac aattgcattc attttatgtt tcaggttcag ggggaggtgt
     2941 gggaggtttt ttaaagcaag taaaacctct acaaatgtgg taaaatcgat aaggatccgt
     3001 cgaccgatgc ccttgagagc cttcaaccca gtcagctcct tccggtgggc gcggggcatg
     3061 actatcgtcg ccgcacttat gactgtcttc tttatcatgc aactcgtagg acaggtgccg
     3121 gcagcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
     3181 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
     3241 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
     3301 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
     3361 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
     3421 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
     3481 ttcgggaagc gtggcgcttt ctcaatgctc acgctgtagg tatctcagtt cggtgtaggt
     3541 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
     3601 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
     3661 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
     3721 gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa
     3781 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
     3841 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
     3901 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
     3961 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
     4021 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
     4081 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
     4141 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
     4201 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
     4261 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
     4321 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
     4381 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
     4441 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
     4501 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
     4561 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
     4621 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
     4681 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
     4741 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
     4801 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
     4861 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
     4921 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
     4981 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
     5041 tccccgaaaa gtgccacctg acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt
     5101 ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt
     5161 cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct
     5221 ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg
     5281 tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga
     5341 gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc
     5401 ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga
     5461 gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgttta caatttccca
     5521 ttcgccattc aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt
     5581 acgccagccc aagctaccat gataagtaag taatattaag gtacgggagg tacttggagc
     5641 ggccgcaata aaatatcttt attttcatta catctgtgtg ttggtttttt gtgtgaatcg
     5701 atagtactaa catacgctct ccatcaaaac aaaacgaaac aaaacaaact agcaaaatag
     5761 gctgtcccca gtgcaagtgc aggtgccaga acatttctct atcgata
//
