LOCUS       62056_11590        4839 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4839)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..4839
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             109..1758
                     /codon_start=1
                     /label=luciferase
                     /note="firefly luciferase"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     polyA_signal    complement(1802..1923)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(2342..2930)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3104..3961)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(3962..4066)
                     /label=AmpR promoter
     rep_origin      4093..4548
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     polyA_signal    4679..4727
                     /label=poly(A) signal
                     /note="synthetic polyadenylation signal"
     misc_feature    4741..4832
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from
                     the human alpha-2 globin gene"
ORIGIN
        1 ggtaccgagc tcttacgcgt gctagcccgg gctcgagtct atatttatat ctatatttat
       61 atctatattt ataaagcttg gcattccggt actgttggta aagccaccat ggaagacgcc
      121 aaaaacataa agaaaggccc ggcgccattc tatccgctgg aagatggaac cgctggagag
      181 caactgcata aggctatgaa gagatacgcc ctggttcctg gaacaattgc ttttacagat
      241 gcacatatcg aggtggacat cacttacgct gagtacttcg aaatgtccgt tcggttggca
      301 gaagctatga aacgatatgg gctgaataca aatcacagaa tcgtcgtatg cagtgaaaac
      361 tctcttcaat tctttatgcc ggtgttgggc gcgttattta tcggagttgc agttgcgccc
      421 gcgaacgaca tttataatga acgtgaattg ctcaacagta tgggcatttc gcagcctacc
      481 gtggtgttcg tttccaaaaa ggggttgcaa aaaattttga acgtgcaaaa aaagctccca
      541 atcatccaaa aaattattat catggattct aaaacggatt accagggatt tcagtcgatg
      601 tacacgttcg tcacatctca tctacctccc ggttttaatg aatacgattt tgtgccagag
      661 tccttcgata gggacaagac aattgcactg atcatgaact cctctggatc tactggtctg
      721 cctaaaggtg tcgctctgcc tcatagaact gcctgcgtga gattctcgca tgccagagat
      781 cctatttttg gcaatcaaat cattccggat actgcgattt taagtgttgt tccattccat
      841 cacggttttg gaatgtttac tacactcgga tatttgatat gtggatttcg agtcgtctta
      901 atgtatagat ttgaagaaga gctgtttctg aggagccttc aggattacaa gattcaaagt
      961 gcgctgctgg tgccaaccct attctccttc ttcgccaaaa gcactctgat tgacaaatac
     1021 gatttatcta atttacacga aattgcttct ggtggcgctc ccctctctaa ggaagtcggg
     1081 gaagcggttg ccaagaggtt ccatctgcca ggtatcaggc aaggatatgg gctcactgag
     1141 actacatcag ctattctgat tacacccgag ggggatgata aaccgggcgc ggtcggtaaa
     1201 gttgttccat tttttgaagc gaaggttgtg gatctggata ccgggaaaac gctgggcgtt
     1261 aatcaaagag gcgaactgtg tgtgagaggt cctatgatta tgtccggtta tgtaaacaat
     1321 ccggaagcga ccaacgcctt gattgacaag gatggatggc tacattctgg agacatagct
     1381 tactgggacg aagacgaaca cttcttcatc gttgaccgcc tgaagtctct gattaagtac
     1441 aaaggctatc aggtggctcc cgctgaattg gaatccatct tgctccaaca ccccaacatc
     1501 ttcgacgcag gtgtcgcagg tcttcccgac gatgacgccg gtgaacttcc cgccgccgtt
     1561 gttgttttgg agcacggaaa gacgatgacg gaaaaagaga tcgtggatta cgtcgccagt
     1621 caagtaacaa ccgcgaaaaa gttgcgcgga ggagttgtgt ttgtggacga agtaccgaaa
     1681 ggtcttaccg gaaaactcga cgcaagaaaa atcagagaga tcctcataaa ggccaagaag
     1741 ggcggaaaga tcgccgtgta attctagagt cggggcggcc ggccgcttcg agcagacatg
     1801 ataagataca ttgatgagtt tggacaaacc acaactagaa tgcagtgaaa aaaatgcttt
     1861 atttgtgaaa tttgtgatgc tattgcttta tttgtaacca ttataagctg caataaacaa
     1921 gttaacaaca acaattgcat tcattttatg tttcaggttc agggggaggt gtgggaggtt
     1981 ttttaaagca agtaaaacct ctacaaatgt ggtaaaatcg ataaggatcc gtcgaccgat
     2041 gcccttgaga gccttcaacc cagtcagctc cttccggtgg gcgcggggca tgactatcgt
     2101 cgccgcactt atgactgtct tctttatcat gcaactcgta ggacaggtgc cggcagcgct
     2161 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
     2221 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
     2281 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
     2341 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
     2401 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
     2461 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
     2521 gcgtggcgct ttctcaatgc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
     2581 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
     2641 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
     2701 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
     2761 ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta
     2821 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
     2881 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
     2941 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
     3001 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
     3061 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
     3121 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
     3181 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
     3241 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
     3301 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
     3361 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
     3421 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
     3481 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
     3541 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
     3601 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
     3661 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
     3721 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
     3781 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
     3841 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
     3901 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
     3961 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
     4021 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
     4081 aagtgccacc tgacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc
     4141 gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt
     4201 cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag
     4261 ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt
     4321 cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt
     4381 tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt
     4441 cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt
     4501 aacaaaaatt taacgcgaat tttaacaaaa tattaacgtt tacaatttcc cattcgccat
     4561 tcaggctgcg caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc
     4621 ccaagctacc atgataagta agtaatatta aggtacggga ggtacttgga gcggccgcaa
     4681 taaaatatct ttattttcat tacatctgtg tgttggtttt ttgtgtgaat cgatagtact
     4741 aacatacgct ctccatcaaa acaaaacgaa acaaaacaaa ctagcaaaat aggctgtccc
     4801 cagtgcaagt gcaggtgcca gaacatttct ctatcgata
//
