LOCUS       40924_21948        6248 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Reporter vector pGL3-3'UTR, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6248)
  AUTHORS   Bi Y, Qiao X, Hua Z, Zhang L, Liu X, Li L, Hua W, Xiao H, Zhou J, 
            Wei Q, Zheng X.
  TITLE     An asymmetric PCR-based, reliable and rapid single-tube native DNA 
            engineering strategy
  JOURNAL   BMC Biotechnol. 12, 39 (2012)
  PUBMED    22768962
REFERENCE   2  (bases 1 to 6248)
  AUTHORS   Bi Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-AUG-2011) Biotechnology Group, Institute of Animal 
            Science and Veterinary, Hubei Academy of Agricultural Science, No. 
            1, Nanhu Yaoyuan, Hongshan District, Wuhan, Hubei 430064, P. R. 
            China
REFERENCE   3  (bases 1 to 6248)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6248)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Biotechnol. 12, 39 (2012)"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (03-AUG-2011) Biotechnology Group, Institute of Animal Science and 
            Veterinary, Hubei Academy of Agricultural Science, No. 1, Nanhu 
            Yaoyuan, Hongshan District, Wuhan, Hubei 430064, P. R. China"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6248
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        48..244
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     CDS             280..1929
                     /label=luciferase
                     /note="firefly luciferase"
     regulatory      1988..3433
                     /regulatory_class="terminator"
     regulatory      2170..2175
                     /regulatory_class="polyA_signal_sequence"
     regulatory      2584..2589
                     /regulatory_class="polyA_signal_sequence"
     regulatory      2908..2913
                     /regulatory_class="polyA_signal_sequence"
     regulatory      3269..3274
                     /regulatory_class="polyA_signal_sequence"
     rep_origin      complement(3751..4339)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4513..5370)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(5371..5475)
                     /label=AmpR promoter
     rep_origin      5502..5957
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     polyA_signal    6088..6136
                     /label=poly(A) signal
                     /note="synthetic polyadenylation signal"
     misc_feature    6150..6241
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from
                     the human alpha-2 globin gene"
ORIGIN
        1 ggtaccgagc tcttacgcgt gctagcccgg gctcgagatc tgcgatctgc atctcaatta
       61 gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc
      121 cgcccattct ccgccccatc gctgactaat tttttttatt tatgcagagg ccgaggccgc
      181 ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg
      241 caaaaagctt ggcattccgg tactgttggt aaagccacca tggaagacgc caaaaacata
      301 aagaaaggcc cggcgccatt ctatccgctg gaagatggaa ccgctggaga gcaactgcat
      361 aaggctatga agagatacgc cctggttcct ggaacaattg cttttacaga tgcacatatc
      421 gaggtggaca tcacttacgc tgagtacttc gaaatgtccg ttcggttggc agaagctatg
      481 aaacgatatg ggctgaatac aaatcacaga atcgtcgtat gcagtgaaaa ctctcttcaa
      541 ttctttatgc cggtgttggg cgcgttattt atcggagttg cagttgcgcc cgcgaacgac
      601 atttataatg aacgtgaatt gctcaacagt atgggcattt cgcagcctac cgtggtgttc
      661 gtttccaaaa aggggttgca aaaaattttg aacgtgcaaa aaaagctccc aatcatccaa
      721 aaaattatta tcatggattc taaaacggat taccagggat ttcagtcgat gtacacgttc
      781 gtcacatctc atctacctcc cggttttaat gaatacgatt ttgtgccaga gtccttcgat
      841 agggacaaga caattgcact gatcatgaac tcctctggat ctactggtct gcctaaaggt
      901 gtcgctctgc ctcatagaac tgcctgcgtg agattctcgc atgccagaga tcctattttt
      961 ggcaatcaaa tcattccgga tactgcgatt ttaagtgttg ttccattcca tcacggtttt
     1021 ggaatgttta ctacactcgg atatttgata tgtggatttc gagtcgtctt aatgtataga
     1081 tttgaagaag agctgtttct gaggagcctt caggattaca agattcaaag tgcgctgctg
     1141 gtgccaaccc tattctcctt cttcgccaaa agcactctga ttgacaaata cgatttatct
     1201 aatttacacg aaattgcttc tggtggcgct cccctctcta aggaagtcgg ggaagcggtt
     1261 gccaagaggt tccatctgcc aggtatcagg caaggatatg ggctcactga gactacatca
     1321 gctattctga ttacacccga gggggatgat aaaccgggcg cggtcggtaa agttgttcca
     1381 ttttttgaag cgaaggttgt ggatctggat accgggaaaa cgctgggcgt taatcaaaga
     1441 ggcgaactgt gtgtgagagg tcctatgatt atgtccggtt atgtaaacaa tccggaagcg
     1501 accaacgcct tgattgacaa ggatggatgg ctacattctg gagacatagc ttactgggac
     1561 gaagacgaac acttcttcat cgttgaccgc ctgaagtctc tgattaagta caaaggctat
     1621 caggtggctc ccgctgaatt ggaatccatc ttgctccaac accccaacat cttcgacgca
     1681 ggtgtcgcag gtcttcccga cgatgacgcc ggtgaacttc ccgccgccgt tgttgttttg
     1741 gagcacggaa agacgatgac ggaaaaagag atcgtggatt acgtcgccag tcaagtaaca
     1801 accgcgaaaa agttgcgcgg aggagttgtg tttgtggacg aagtaccgaa aggtcttacc
     1861 ggaaaactcg acgcaagaaa aatcagagag atcctcataa aggccaagaa gggcggaaag
     1921 atcgccgtgt aattctagag tcggggcggc cggccgcttc gagcagacat gataagatac
     1981 attgatgggt tcattacttc ctaaaacatg gaaggttttt cccctcaata attttgaaac
     2041 tgtgaaatta tgtaccacag gctacatgcc tggagtatgc tacagtcact taagcacaag
     2101 cgacagtata tgaactaaaa gagagaatat atgcaatggt tggcatttaa ccatcaaaac
     2161 aaatcataca ataaaaagtt tttatgattt ccagagtttt tgaacgagga gatcaaattc
     2221 catttatgtt catatatatt acaacatatg caggtaaatg aaagcaattc tccttgtgtt
     2281 ctggtgaatt aaaggagtat gctttaaagt ctatttcttt acaattttac ttaatattta
     2341 cagaaaaatc tatatgtagt attgataaga tgtaggattg ttatatacca ttatttgagt
     2401 catccttaaa cacttgaatt atattgtatg atagcatact tggtaagaga agattccaca
     2461 aaaataggga tggtacacca tatgcaagtt cccattccta ttctgattga tacagtacat
     2521 tgacaattca tgccaatggt gctaattcaa taggctgaat ggctggtgtt atcaggttta
     2581 tcaaataaaa gcattcagta aagtaatggt tctcctttct tcaggtgcat tttcatactc
     2641 tttcaaatgg agaatggatt ttctttaatg aaagaaaaat catttttcta gagctctgca
     2701 ttcaattctg tagcatactt ggagaaactg catttaaaag gcagccaaaa agtattcatt
     2761 tttatcaaaa tttcaaaatt atagcctgcc tttgcaacac tgcagttttt atgataaaat
     2821 catggcaatg actgattcta tcaatattgt ataaaaagac tttgaaacaa ttgcatttat
     2881 ataatatgta aacaatattg ttttgtaaat aaatgtctcc ttttttattt actttggtat
     2941 atttttatgt aaggatattt caaattaagt attaaggcac aaatacatgt catgtgacag
     3001 aaaagcaaat gcttatattt cggagcaaat tagctgatta aatagtggtc ttaaaactcc
     3061 atatgctaat ggttagatgg ttatattaca atcattttat atttttttac attattaaca
     3121 ttcactatgg attcatgatg gctgtataat gtgaatgtga aatttcaatg gtttactgtc
     3181 aatgtattca aatctcaacg ttccattatt ttaatactta taagtaagca taccaaaatg
     3241 atttaactca attatctgaa atcagaataa taaactgatg atatcttaag aattgttaat
     3301 ttaattttat aattcgataa tgaatatatt tctccatata tttacttcta ttttgtaaat
     3361 taggattttg ttaatcaaat acattgtact tatgactaag tgaaattatt tcttacatct
     3421 aatgtgtaga aacggatccg tcgaccgatg cccttgagag ccttcaaccc agtcagctcc
     3481 ttccggtggg cgcggggcat gactatcgtc gccgcactta tgactgtctt ctttatcatg
     3541 caactcgtag gacaggtgcc ggcagcgctc ttccgcttcc tcgctcactg actcgctgcg
     3601 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
     3661 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
     3721 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
     3781 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
     3841 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
     3901 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
     3961 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
     4021 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
     4081 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
     4141 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
     4201 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
     4261 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
     4321 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
     4381 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
     4441 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
     4501 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
     4561 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
     4621 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc
     4681 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
     4741 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
     4801 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
     4861 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
     4921 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
     4981 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
     5041 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
     5101 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
     5161 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
     5221 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac
     5281 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
     5341 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
     5401 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
     5461 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgcgccct gtagcggcgc
     5521 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct
     5581 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg
     5641 tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga
     5701 ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt
     5761 ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg
     5821 aacaacactc aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc
     5881 ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat
     5941 attaacgctt acaatttgcc attcgccatt caggctgcgc aactgttggg aagggcgatc
     6001 ggtgcgggcc tcttcgctat tacgccagcc caagctacca tgataagtaa gtaatattaa
     6061 ggtacgggag gtacttggag cggccgcaat aaaatatctt tattttcatt acatctgtgt
     6121 gttggttttt tgtgtgaatc gatagtacta acatacgctc tccatcaaaa caaaacgaaa
     6181 caaaacaaac tagcaaaata ggctgtcccc agtgcaagtg caggtgccag aacatttctc
     6241 tatcgata
//
