LOCUS       40924_21943        5021 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Reporter vector pGL3(R2.2), complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5021)
  AUTHORS   Almond BD, Chauvin F, Kenefick KB.
  TITLE     Rapid Response (TM) Reporter Vectors Technical Manual, TM242
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5021)
  AUTHORS   Almond BD, Chauvin F, Kenefick KB.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-NOV-2003) Scientific Communications, Promega 
            Corporation, 2800 Woods Hollow Rd., Madison, WI 53711, USA
REFERENCE   3  (bases 1 to 5021)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5021)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (26-NOV-2003) Scientific Communications, Promega Corporation, 2800 
            Woods Hollow Rd., Madison, WI 53711, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5021
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..58
                     /label=multiple cloning region
                     /note="multiple cloning region"
     CDS             88..1737
                     /codon_start=1
                     /label=luciferase
                     /note="firefly luciferase"
                     /translation="MADAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     CDS             1744..1791
                     /codon_start=1
                     /label=hCL1
                     /note="non-ORF yeast peptide conferring ubiquitin-dependent
                     degradation"
                     /translation="ACKNWFSSLSHFVIHL"
     CDS             1795..1914
                     /codon_start=1
                     /label=hPEST
                     /note="PEST degradation sequence from mouse ornithine 
                     decarboxylase"
                     /translation="SHGFPPEVEEQAAGTLPMSCAQESGMDRHPAACASARINV"
     polyA_signal    complement(1984..2105)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     2264..2283
                     /label=reporter vector primer 4 (RVPrimer4)
                     /note="reporter vector primer 4 (RVPrimer4)"
     rep_origin      2521
                     /label=ColE1-derived
                     /note="ColE1-derived"
     rep_origin      complement(2524..3112)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3286..4143)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(4144..4248)
                     /label=AmpR promoter
     rep_origin      4275..4730
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     polyA_signal    4861..4909
                     /label=poly(A) signal
                     /note="synthetic polyadenylation signal"
     misc_feature    4923..5014
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from
                     the human alpha-2 globin gene"
ORIGIN
        1 ggtaccgagc tcttacgcgt gctagcccgg gctcgagatc tgcgatctaa gtaagcttgg
       61 cattccggta ctgttggtaa agccaccatg gccgatgcta agaacattaa gaagggccct
      121 gctcccttct accctctgga ggatggcacc gctggcgagc agctgcacaa ggccatgaag
      181 aggtatgccc tggtgcctgg caccattgcc ttcaccgatg cccacattga ggtggacatc
      241 acctatgccg agtacttcga gatgtctgtg cgcctggccg aggccatgaa gaggtacggc
      301 ctgaacacca accaccgcat cgtggtgtgc tctgagaact ctctgcagtt cttcatgcca
      361 gtgctgggcg ccctgttcat cggagtggcc gtggcccctg ctaacgacat ttacaacgag
      421 cgcgagctgc tgaacagcat gggcatttct cagcctaccg tggtgttcgt gtctaagaag
      481 ggcctgcaga agatcctgaa cgtgcagaag aagctgccta tcatccagaa gatcatcatc
      541 atggactcta agaccgacta ccagggcttc cagagcatgt acacattcgt gacatctcat
      601 ctgcctcctg gcttcaacga gtacgacttc gtgccagagt ctttcgacag ggacaaaacc
      661 attgccctga tcatgaacag ctctgggtct accggcctgc ctaagggcgt ggccctgcct
      721 catcgcaccg cctgtgtgcg cttctctcac gcccgcgacc ctattttcgg caaccagatc
      781 atccccgaca ccgctattct gagcgtggtg ccattccacc acggcttcgg catgttcacc
      841 accctgggct acctgatttg cggctttcgg gtggtgctga tgtaccgctt cgaggaggag
      901 ctgttcctgc gcagcctgca agactacaaa attcagtctg ccctgctggt gccaaccctg
      961 ttcagcttct tcgctaagag caccctgatc gacaagtacg acctgtctaa cctgcacgag
     1021 attgcctctg gcggcgcccc actgtctaag gaggtgggcg aagccgtggc caagcgcttt
     1081 catctgccag gcatccgcca gggctacggc ctgaccgaga caaccagcgc cattctgatt
     1141 accccagagg gcgacgacaa gcctggcgcc gtgggcaagg tggtgccatt cttcgaggcc
     1201 aaggtggtgg acctggacac cggcaagacc ctgggagtga accagcgcgg cgagctgtgt
     1261 gtgcgcggcc ctatgattat gtccggctac gtgaataacc ctgaggccac aaacgccctg
     1321 atcgacaagg acggctggct gcactctggc gacattgcct actgggacga ggacgagcac
     1381 ttcttcatcg tggaccgcct gaagtctctg atcaagtaca agggctacca ggtggcccca
     1441 gccgagctgg agtctatcct gctgcagcac cctaacattt tcgacgccgg agtggccggc
     1501 ctgcccgacg acgatgccgg cgagctgcct gccgccgtcg tcgtgctgga acacggcaag
     1561 accatgaccg agaaggagat cgtggactat gtggccagcc aggtgacaac cgccaagaag
     1621 ctgcgcggcg gagtggtgtt cgtggacgag gtgcccaagg gcctgaccgg caagctggac
     1681 gcccgcaaga tccgcgagat cctgatcaag gctaagaaag gcggcaagat cgccgtgaat
     1741 tctgcttgca agaactggtt cagtagctta agccactttg tgatccacct taacagccac
     1801 ggcttccctc ccgaggtgga ggagcaggcc gccggcaccc tgcccatgag ctgcgcccag
     1861 gagagcggca tggatagaca ccctgctgct tgcgccagcg ccaggatcaa cgtctagggc
     1921 gcggacttta tttatttatt tctttctaga gtcggggcgg ccggccgctt cgagcagaca
     1981 tgataagata cattgatgag tttggacaaa ccacaactag aatgcagtga aaaaaatgct
     2041 ttatttgtga aatttgtgat gctattgctt tatttgtaac cattataagc tgcaataaac
     2101 aagttaacaa caacaattgc attcatttta tgtttcaggt tcagggggag gtgtgggagg
     2161 ttttttaaag caagtaaaac ctctacaaat gtggtaaaat cgataaggat ccgtcgaccg
     2221 atgcccttga gagccttcaa cccagtcagc tccttccggt gggcgcgggg catgactatc
     2281 gtcgccgcac ttatgactgt cttctttatc atgcaactcg taggacaggt gccggcagcg
     2341 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
     2401 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
     2461 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
     2521 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     2581 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     2641 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     2701 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     2761 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     2821 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     2881 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     2941 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt
     3001 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     3061 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
     3121 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
     3181 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
     3241 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
     3301 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
     3361 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
     3421 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
     3481 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
     3541 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac
     3601 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
     3661 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
     3721 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
     3781 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
     3841 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat
     3901 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
     3961 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
     4021 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
     4081 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
     4141 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
     4201 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
     4261 aaaagtgcca cctgacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac
     4321 gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc
     4381 ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt
     4441 agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg
     4501 ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac
     4561 gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta
     4621 ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat
     4681 ttaacaaaaa tttaacgcga attttaacaa aatattaacg cttacaattt gccattcgcc
     4741 attcaggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc tattacgcca
     4801 gcccaagcta ccatgataag taagtaatat taaggtacgg gaggtacttg gagcggccgc
     4861 aataaaatat ctttattttc attacatctg tgtgttggtt ttttgtgtga atcgatagta
     4921 ctaacatacg ctctccatca aaacaaaacg aaacaaaaca aactagcaaa ataggctgtc
     4981 cccagtgcaa gtgcaggtgc cagaacattt ctctatcgat a
//
