LOCUS       40924_21938        4949 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Reporter vector pGL3(R2.1), complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4949)
  AUTHORS   Almond BD, Chauvin F, Kenefick KB.
  TITLE     Rapid Response (TM) Reporter Vectors Technical Manual, TM242
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4949)
  AUTHORS   Almond BD, Chauvin F, Kenefick KB.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-NOV-2003) Scientific Communications, Promega 
            Corporation, 2800 Woods Hollow Rd., Madison, WI 53711, USA
REFERENCE   3  (bases 1 to 4949)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4949)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (26-NOV-2003) Scientific Communications, Promega Corporation, 2800 
            Woods Hollow Rd., Madison, WI 53711, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4949
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..58
                     /label=multiple cloning region
                     /note="multiple cloning region"
     CDS             88..1737
                     /codon_start=1
                     /label=luciferase
                     /note="firefly luciferase"
                     /translation="MADAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     CDS             1741..1860
                     /codon_start=1
                     /label=hPEST
                     /note="PEST degradation sequence from mouse ornithine 
                     decarboxylase"
                     /translation="SHGFPPEVEEQAAGTLPMSCAQESGMDRHPAACASARINV"
     polyA_signal    complement(1912..2033)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     2192..2211
                     /label=reporter vector primer 4 (RVPrimer4)
                     /note="reporter vector primer 4 (RVPrimer4)"
     rep_origin      2449
                     /label=ColE1-derived
                     /note="ColE1-derived"
     rep_origin      complement(2452..3040)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3214..4071)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(4072..4176)
                     /label=AmpR promoter
     rep_origin      4203..4658
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     polyA_signal    4789..4837
                     /label=poly(A) signal
                     /note="synthetic polyadenylation signal"
     misc_feature    4851..4942
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from
                     the human alpha-2 globin gene"
ORIGIN
        1 ggtaccgagc tcttacgcgt gctagcccgg gctcgagatc tgcgatctaa gtaagcttgg
       61 cattccggta ctgttggtaa agccaccatg gccgatgcta agaacattaa gaagggccct
      121 gctcccttct accctctgga ggatggcacc gctggcgagc agctgcacaa ggccatgaag
      181 aggtatgccc tggtgcctgg caccattgcc ttcaccgatg cccacattga ggtggacatc
      241 acctatgccg agtacttcga gatgtctgtg cgcctggccg aggccatgaa gaggtacggc
      301 ctgaacacca accaccgcat cgtggtgtgc tctgagaact ctctgcagtt cttcatgcca
      361 gtgctgggcg ccctgttcat cggagtggcc gtggcccctg ctaacgacat ttacaacgag
      421 cgcgagctgc tgaacagcat gggcatttct cagcctaccg tggtgttcgt gtctaagaag
      481 ggcctgcaga agatcctgaa cgtgcagaag aagctgccta tcatccagaa gatcatcatc
      541 atggactcta agaccgacta ccagggcttc cagagcatgt acacattcgt gacatctcat
      601 ctgcctcctg gcttcaacga gtacgacttc gtgccagagt ctttcgacag ggacaaaacc
      661 attgccctga tcatgaacag ctctgggtct accggcctgc ctaagggcgt ggccctgcct
      721 catcgcaccg cctgtgtgcg cttctctcac gcccgcgacc ctattttcgg caaccagatc
      781 atccccgaca ccgctattct gagcgtggtg ccattccacc acggcttcgg catgttcacc
      841 accctgggct acctgatttg cggctttcgg gtggtgctga tgtaccgctt cgaggaggag
      901 ctgttcctgc gcagcctgca agactacaaa attcagtctg ccctgctggt gccaaccctg
      961 ttcagcttct tcgctaagag caccctgatc gacaagtacg acctgtctaa cctgcacgag
     1021 attgcctctg gcggcgcccc actgtctaag gaggtgggcg aagccgtggc caagcgcttt
     1081 catctgccag gcatccgcca gggctacggc ctgaccgaga caaccagcgc cattctgatt
     1141 accccagagg gcgacgacaa gcctggcgcc gtgggcaagg tggtgccatt cttcgaggcc
     1201 aaggtggtgg acctggacac cggcaagacc ctgggagtga accagcgcgg cgagctgtgt
     1261 gtgcgcggcc ctatgattat gtccggctac gtgaataacc ctgaggccac aaacgccctg
     1321 atcgacaagg acggctggct gcactctggc gacattgcct actgggacga ggacgagcac
     1381 ttcttcatcg tggaccgcct gaagtctctg atcaagtaca agggctacca ggtggcccca
     1441 gccgagctgg agtctatcct gctgcagcac cctaacattt tcgacgccgg agtggccggc
     1501 ctgcccgacg acgatgccgg cgagctgcct gccgccgtcg tcgtgctgga acacggcaag
     1561 accatgaccg agaaggagat cgtggactat gtggccagcc aggtgacaac cgccaagaag
     1621 ctgcgcggcg gagtggtgtt cgtggacgag gtgcccaagg gcctgaccgg caagctggac
     1681 gcccgcaaga tccgcgagat cctgatcaag gctaagaaag gcggcaagat cgccgtgaat
     1741 tctcacggct tccctcccga ggtggaggag caggccgccg gcaccctgcc catgagctgc
     1801 gcccaggaga gcggcatgga tagacaccct gctgcttgcg ccagcgccag gatcaacgtc
     1861 taaggccgcg actctagagt cggggcggcc ggccgcttcg agcagacatg ataagataca
     1921 ttgatgagtt tggacaaacc acaactagaa tgcagtgaaa aaaatgcttt atttgtgaaa
     1981 tttgtgatgc tattgcttta tttgtaacca ttataagctg caataaacaa gttaacaaca
     2041 acaattgcat tcattttatg tttcaggttc agggggaggt gtgggaggtt ttttaaagca
     2101 agtaaaacct ctacaaatgt ggtaaaatcg ataaggatcc gtcgaccgat gcccttgaga
     2161 gccttcaacc cagtcagctc cttccggtgg gcgcggggca tgactatcgt cgccgcactt
     2221 atgactgtct tctttatcat gcaactcgta ggacaggtgc cggcagcgct cttccgcttc
     2281 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
     2341 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
     2401 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
     2461 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
     2521 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
     2581 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
     2641 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
     2701 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
     2761 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
     2821 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
     2881 ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
     2941 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
     3001 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
     3061 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
     3121 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta
     3181 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat
     3241 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac
     3301 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg
     3361 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag
     3421 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt
     3481 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt
     3541 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt
     3601 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt
     3661 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct
     3721 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt
     3781 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac
     3841 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa
     3901 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa
     3961 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca
     4021 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct
     4081 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga
     4141 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc
     4201 tgacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac
     4261 cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc
     4321 cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt
     4381 tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg
     4441 gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag
     4501 tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt
     4561 ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt
     4621 taacgcgaat tttaacaaaa tattaacgct tacaatttgc cattcgccat tcaggctgcg
     4681 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc ccaagctacc
     4741 atgataagta agtaatatta aggtacggga ggtacttgga gcggccgcaa taaaatatct
     4801 ttattttcat tacatctgtg tgttggtttt ttgtgtgaat cgatagtact aacatacgct
     4861 ctccatcaaa acaaaacgaa acaaaacaaa ctagcaaaat aggctgtccc cagtgcaagt
     4921 gcaggtgcca gaacatttct ctatcgata
//
