LOCUS       pGL2-Enhancer.        5855 bp DNA     circular SYN 01-JAN-1980
DEFINITION  SV40 enhancer-containing vector for measuring the activity of 
            promoter sequences with a luciferase assay.
ACCESSION   .
VERSION     .
KEYWORDS    pGL2-Enhancer
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5855)
  AUTHORS   Promega
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 5855)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5855
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..52
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     CDS             76..1725
                     /label=luciferase
                     /note="firefly luciferase"
     intron          1969..2034
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             2164..2184
                     /label=SV40 NLS
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     polyA_signal    2609..2743
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     enhancer        2755..2984
                     /note="SV40 enhancer"
     rep_origin      complement(3313..3901)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4075..4932)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(4933..5037)
                     /label=AmpR promoter
     rep_origin      5064..5519
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     polyA_signal    5709..5830
                     /label=SV40 poly(A) signal
                     /note="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
ORIGIN
        1 cccgggaggt accgagctct tacgcgtgct agctcgagat ctaagtaagc ttggcattcc
       61 ggtactgttg gtaaaatgga agacgccaaa aacataaaga aaggcccggc gccattctat
      121 cctctagagg atggaaccgc tggagagcaa ctgcataagg ctatgaagag atacgccctg
      181 gttcctggaa caattgcttt tacagatgca catatcgagg tgaacatcac gtacgcggaa
      241 tacttcgaaa tgtccgttcg gttggcagaa gctatgaaac gatatgggct gaatacaaat
      301 cacagaatcg tcgtatgcag tgaaaactct cttcaattct ttatgccggt gttgggcgcg
      361 ttatttatcg gagttgcagt tgcgcccgcg aacgacattt ataatgaacg tgaattgctc
      421 aacagtatga acatttcgca gcctaccgta gtgtttgttt ccaaaaaggg gttgcaaaaa
      481 attttgaacg tgcaaaaaaa attaccaata atccagaaaa ttattatcat ggattctaaa
      541 acggattacc agggatttca gtcgatgtac acgttcgtca catctcatct acctcccggt
      601 tttaatgaat acgattttgt accagagtcc tttgatcgtg acaaaacaat tgcactgata
      661 atgaattcct ctggatctac tgggttacct aagggtgtgg cccttccgca tagaactgcc
      721 tgcgtcagat tctcgcatgc cagagatcct atttttggca atcaaatcat tccggatact
      781 gcgattttaa gtgttgttcc attccatcac ggttttggaa tgtttactac actcggatat
      841 ttgatatgtg gatttcgagt cgtcttaatg tatagatttg aagaagagct gtttttacga
      901 tcccttcagg attacaaaat tcaaagtgcg ttgctagtac caaccctatt ttcattcttc
      961 gccaaaagca ctctgattga caaatacgat ttatctaatt tacacgaaat tgcttctggg
     1021 ggcgcacctc tttcgaaaga agtcggggaa gcggttgcaa aacgcttcca tcttccaggg
     1081 atacgacaag gatatgggct cactgagact acatcagcta ttctgattac acccgagggg
     1141 gatgataaac cgggcgcggt cggtaaagtt gttccatttt ttgaagcgaa ggttgtggat
     1201 ctggataccg ggaaaacgct gggcgttaat cagagaggcg aattatgtgt cagaggacct
     1261 atgattatgt ccggttatgt aaacaatccg gaagcgacca acgccttgat tgacaaggat
     1321 ggatggctac attctggaga catagcttac tgggacgaag acgaacactt cttcatagtt
     1381 gaccgcttga agtctttaat taaatacaaa ggatatcagg tggcccccgc tgaattggaa
     1441 tcgatattgt tacaacaccc caacatcttc gacgcgggcg tggcaggtct tcccgacgat
     1501 gacgccggtg aacttcccgc cgccgttgtt gttttggagc acggaaagac gatgacggaa
     1561 aaagagatcg tggattacgt cgccagtcaa gtaacaaccg cgaaaaagtt gcgcggagga
     1621 gttgtgtttg tggacgaagt accgaaaggt cttaccggaa aactcgacgc aagaaaaatc
     1681 agagagatcc tcataaaggc caagaagggc ggaaagtcca aattgtaaaa tgtaactgta
     1741 ttcagcgatg acgaaattct tagctattgt aatactgcga tgagtggcag ggcggggcgt
     1801 aattttttta aggcagttat tggtgccctt aaacgcctgg ttgctacgcc tgaataagtg
     1861 ataataagcg gatgaatggc agaaattcgc cggatctttg tgaaggaacc ttacttctgt
     1921 ggtgtgacat aattggacaa actacctaca gagatttaaa gctctaaggt aaatataaaa
     1981 tttttaagtg tataatgtgt taaactactg attctaattg tttgtgtatt ttagattcca
     2041 acctatggaa ctgatgaatg ggagcagtgg tggaatgcct ttaatgagga aaacctgttt
     2101 tgctcagaag aaatgccatc tagtgatgat gaggctactg ctgactctca acattctact
     2161 cctccaaaaa agaagagaaa ggtagaagac cccaaggact ttccttcaga attgctaagt
     2221 tttttgagtc atgctgtgtt tagtaataga actcttgctt gctttgctat ttacaccaca
     2281 aaggaaaaag ctgcactgct atacaagaaa attatggaaa aatattctgt aacctttata
     2341 agtaggcata acagttataa tcataacata ctgttttttc ttactccaca caggcataga
     2401 gtgtctgcta ttaataacta tgctcaaaaa ttgtgtacct ttagcttttt aatttgtaaa
     2461 ggggttaata aggaatattt gatgtatagt gccttgacta gagatcataa tcagccatac
     2521 cacatttgta gaggttttac ttgctttaaa aaacctccca cacctccccc tgaacctgaa
     2581 acataaaatg aatgcaattg ttgttgttaa cttgtttatt gcagcttata atggttacaa
     2641 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg
     2701 tggtttgtcc aaactcatca atgtatctta tcatgtctgg atctgaacca tggagcggag
     2761 aatgggcgga actgggcgga gttaggggcg ggatgggcgg agttaggggc gggactatgg
     2821 ttgctgacta attgagatgc atgctttgca tacttctgcc tgctggggag cctggggact
     2881 ttccacacct ggttgctgac taattgagat gcatgctttg catacttctg cctgctgggg
     2941 agcctgggga ctttccacac cctaactgac acacattcca cagcagatcc tctagggatc
     3001 cgtcgaccga tgcccttgag agccttcaac ccagtcagct ccttccggtg ggcgcggggc
     3061 atgactatcg tcgccgcact tatgactgtc ttctttatca tgcaactcgt aggacaggtg
     3121 ccggcagcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
     3181 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
     3241 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
     3301 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
     3361 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     3421 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     3481 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
     3541 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     3601 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
     3661 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
     3721 gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct
     3781 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     3841 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
     3901 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
     3961 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
     4021 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
     4081 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
     4141 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
     4201 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc
     4261 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
     4321 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
     4381 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
     4441 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
     4501 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
     4561 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
     4621 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
     4681 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
     4741 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
     4801 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
     4861 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
     4921 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
     4981 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
     5041 atttccccga aaagtgccac ctgacgcgcc ctgtagcggc gcattaagcg cggcgggtgt
     5101 ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc
     5161 tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg
     5221 gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta
     5281 gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt
     5341 ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat
     5401 ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa
     5461 tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa atattaacgc ttacaatttg
     5521 ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct
     5581 attacgccag cccaagctac catgataagt aagtaatatt aaggtacgtg gaggttttac
     5641 ttgctttaaa aaacctccca cacctccccc tgaacctgaa acataaaatg aatgcaattg
     5701 ttgttgttaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa
     5761 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca
     5821 atgtatctta tggtactgta actgagctaa cataa
//
