LOCUS       pGL2-Control.        6047 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Control vector with the SV40 enhancer and promoter driving strong 
            luciferase expression.
ACCESSION   .
VERSION     .
KEYWORDS    pGL2-Control
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6047)
  AUTHORS   Promega
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 6047)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6047
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..42
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     promoter        42..238
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     CDS             268..1917
                     /label=luciferase
                     /note="firefly luciferase"
     intron          2161..2226
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             2356..2376
                     /label=SV40 NLS
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     polyA_signal    2801..2935
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     enhancer        2947..3176
                     /note="SV40 enhancer"
     rep_origin      complement(3505..4093)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4267..5124)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(5125..5229)
                     /label=AmpR promoter
     rep_origin      5256..5711
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     polyA_signal    5901..6022
                     /label=SV40 poly(A) signal
                     /note="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
ORIGIN
        1 cccgggaggt accgagctct tacgcgtgct agctcgagat ctgcatctca attagtcagc
       61 aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca
      121 ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc
      181 ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa
      241 gcttggcatt ccggtactgt tggtaaaatg gaagacgcca aaaacataaa gaaaggcccg
      301 gcgccattct atcctctaga ggatggaacc gctggagagc aactgcataa ggctatgaag
      361 agatacgccc tggttcctgg aacaattgct tttacagatg cacatatcga ggtgaacatc
      421 acgtacgcgg aatacttcga aatgtccgtt cggttggcag aagctatgaa acgatatggg
      481 ctgaatacaa atcacagaat cgtcgtatgc agtgaaaact ctcttcaatt ctttatgccg
      541 gtgttgggcg cgttatttat cggagttgca gttgcgcccg cgaacgacat ttataatgaa
      601 cgtgaattgc tcaacagtat gaacatttcg cagcctaccg tagtgtttgt ttccaaaaag
      661 gggttgcaaa aaattttgaa cgtgcaaaaa aaattaccaa taatccagaa aattattatc
      721 atggattcta aaacggatta ccagggattt cagtcgatgt acacgttcgt cacatctcat
      781 ctacctcccg gttttaatga atacgatttt gtaccagagt cctttgatcg tgacaaaaca
      841 attgcactga taatgaattc ctctggatct actgggttac ctaagggtgt ggcccttccg
      901 catagaactg cctgcgtcag attctcgcat gccagagatc ctatttttgg caatcaaatc
      961 attccggata ctgcgatttt aagtgttgtt ccattccatc acggttttgg aatgtttact
     1021 acactcggat atttgatatg tggatttcga gtcgtcttaa tgtatagatt tgaagaagag
     1081 ctgtttttac gatcccttca ggattacaaa attcaaagtg cgttgctagt accaacccta
     1141 ttttcattct tcgccaaaag cactctgatt gacaaatacg atttatctaa tttacacgaa
     1201 attgcttctg ggggcgcacc tctttcgaaa gaagtcgggg aagcggttgc aaaacgcttc
     1261 catcttccag ggatacgaca aggatatggg ctcactgaga ctacatcagc tattctgatt
     1321 acacccgagg gggatgataa accgggcgcg gtcggtaaag ttgttccatt ttttgaagcg
     1381 aaggttgtgg atctggatac cgggaaaacg ctgggcgtta atcagagagg cgaattatgt
     1441 gtcagaggac ctatgattat gtccggttat gtaaacaatc cggaagcgac caacgccttg
     1501 attgacaagg atggatggct acattctgga gacatagctt actgggacga agacgaacac
     1561 ttcttcatag ttgaccgctt gaagtcttta attaaataca aaggatatca ggtggccccc
     1621 gctgaattgg aatcgatatt gttacaacac cccaacatct tcgacgcggg cgtggcaggt
     1681 cttcccgacg atgacgccgg tgaacttccc gccgccgttg ttgttttgga gcacggaaag
     1741 acgatgacgg aaaaagagat cgtggattac gtcgccagtc aagtaacaac cgcgaaaaag
     1801 ttgcgcggag gagttgtgtt tgtggacgaa gtaccgaaag gtcttaccgg aaaactcgac
     1861 gcaagaaaaa tcagagagat cctcataaag gccaagaagg gcggaaagtc caaattgtaa
     1921 aatgtaactg tattcagcga tgacgaaatt cttagctatt gtaatactgc gatgagtggc
     1981 agggcggggc gtaatttttt taaggcagtt attggtgccc ttaaacgcct ggttgctacg
     2041 cctgaataag tgataataag cggatgaatg gcagaaattc gccggatctt tgtgaaggaa
     2101 ccttacttct gtggtgtgac ataattggac aaactaccta cagagattta aagctctaag
     2161 gtaaatataa aatttttaag tgtataatgt gttaaactac tgattctaat tgtttgtgta
     2221 ttttagattc caacctatgg aactgatgaa tgggagcagt ggtggaatgc ctttaatgag
     2281 gaaaacctgt tttgctcaga agaaatgcca tctagtgatg atgaggctac tgctgactct
     2341 caacattcta ctcctccaaa aaagaagaga aaggtagaag accccaagga ctttccttca
     2401 gaattgctaa gttttttgag tcatgctgtg tttagtaata gaactcttgc ttgctttgct
     2461 atttacacca caaaggaaaa agctgcactg ctatacaaga aaattatgga aaaatattct
     2521 gtaaccttta taagtaggca taacagttat aatcataaca tactgttttt tcttactcca
     2581 cacaggcata gagtgtctgc tattaataac tatgctcaaa aattgtgtac ctttagcttt
     2641 ttaatttgta aaggggttaa taaggaatat ttgatgtata gtgccttgac tagagatcat
     2701 aatcagccat accacatttg tagaggtttt acttgcttta aaaaacctcc cacacctccc
     2761 cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt aacttgttta ttgcagctta
     2821 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact
     2881 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggatctgaac
     2941 catggagcgg agaatgggcg gaactgggcg gagttagggg cgggatgggc ggagttaggg
     3001 gcgggactat ggttgctgac taattgagat gcatgctttg catacttctg cctgctgggg
     3061 agcctgggga ctttccacac ctggttgctg actaattgag atgcatgctt tgcatacttc
     3121 tgcctgctgg ggagcctggg gactttccac accctaactg acacacattc cacagcagat
     3181 cctctaggga tccgtcgacc gatgcccttg agagccttca acccagtcag ctccttccgg
     3241 tgggcgcggg gcatgactat cgtcgccgca cttatgactg tcttctttat catgcaactc
     3301 gtaggacagg tgccggcagc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt
     3361 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
     3421 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
     3481 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
     3541 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
     3601 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
     3661 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
     3721 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
     3781 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
     3841 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
     3901 tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat
     3961 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
     4021 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
     4081 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
     4141 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
     4201 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga
     4261 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc
     4321 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg
     4381 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat
     4441 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat
     4501 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg
     4561 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc
     4621 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa
     4681 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc
     4741 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt
     4801 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag
     4861 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt
     4921 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag
     4981 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac
     5041 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc
     5101 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca
     5161 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg
     5221 ggttccgcgc acatttcccc gaaaagtgcc acctgacgcg ccctgtagcg gcgcattaag
     5281 cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc
     5341 cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc
     5401 tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa
     5461 aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg
     5521 ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac
     5581 actcaaccct atctcggtct attcttttga tttataaggg attttgccga tttcggccta
     5641 ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac
     5701 gcttacaatt tgccattcgc cattcaggct gcgcaactgt tgggaagggc gatcggtgcg
     5761 ggcctcttcg ctattacgcc agcccaagct accatgataa gtaagtaata ttaaggtacg
     5821 tggaggtttt acttgcttta aaaaacctcc cacacctccc cctgaacctg aaacataaaa
     5881 tgaatgcaat tgttgttgtt aacttgttta ttgcagctta taatggttac aaataaagca
     5941 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
     6001 ccaaactcat caatgtatct tatggtactg taactgagct aacataa
//
