LOCUS       V005976                 3284 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V005976
VERSION     V005976
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 3284)
  AUTHORS   Ming-Ming Y, Wei-Wei Z, Xi-Feng Z, Pei-Lin C.
  TITLE     Construction and characterization of a novel maltose inducible
            expression vector in Bacillus subtilis
  JOURNAL   Biotechnol. Lett. 28 (21), 1713-1718 (2006)
   PUBMED   17001500
REFERENCE   2  (bases 1 to 3284)
  AUTHORS   Yang MM, Zhang WW.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2015) College of Animal Sciences and Technology,
            Northwest A
REFERENCE   3  (bases 1 to 3284)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3284)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol.
            Lett."; date: "2006"; volume: "28"; issue: "21"; pages: "1713-1718"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (15-DEC-2015) College of Animal Sciences and Technology, Northwest
            A"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3284
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1193..1837
                     /gene="cat"
                     /label="Chloramphenicol acetyltransferase"
                     /note="Chloramphenicol acetyltransferase from
                     Staphylococcus aureus. Accession#: P00486"
     rep_origin      complement(2003..2591)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     primer_bind     2643..2659
                     /label="KS primer"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     primer_bind     complement(2693..2709)
                     /label="SK primer"
                     /note="common sequencing primer, one of multiple similar
                     variants"
ORIGIN
        1 tgagttgttt ttataatctt gtatatttag atattaaacg atatttaaat atacatcaag
       61 atatatattt gggtgagcga ttccttaaac gaaattgaga ttaaggagtc gattttttat
      121 gtataaaaac aatcatgcaa atcattcaaa tcatttggaa aatcacgatt tagacaattt
      181 ttctaaaacc ggctactcta atagccggtt aagtggtaat ttttttacca cccctcaacc
      241 agaattaagt tttgatgcta tgacaatcgt tgggaatttg aacaaaacta acgctaaaaa
      301 gctatctgat tttatgagta cagagccaca aataaggctt tgggatatat tacaaacaaa
      361 gtttaaagct aaagcacttc aagaaaaggt ttatatcgaa tatgacaaag taaaagcaga
      421 tagttgggat agacgtaata tgcgtgttga atttaatcca aataaactca cacatgaaga
      481 aatgctttgg ttaaaacaaa atattatcga ctacatggaa gatgacggtt ttacgaggtt
      541 agatttagct tttgattttg aagatgattt gagtgattac tacgcaatga ctgataaagc
      601 agttaagaaa actatttttt atggtcgtaa tggtaagcca gaaacaaaat attttggtgt
      661 tcgtgacagt gatagattta ttagaattta taataaaaaa caagaacgta aagataacgc
      721 cgatgttgaa gttatgtctg aacatttatg gcgtgtagaa attgaattga aaagagatat
      781 ggttgattac tggaatgatt gctttgatga tttacacatt ttgaaacccg attggacaac
      841 accagaaaaa gtaaaagaac aagcaatggt ttatttgtta ctgaatgaag aaggcacgtg
      901 gggaaaactt gaaagacatg ctaaatataa atacaagcaa ttgattaaag aaatatctcc
      961 aattgattta acggaattaa tgaaatcgac tttaaaagag aatgaaaagc aattgcaaaa
     1021 acagattgat ttttggcaac gtgaatttag attttggaag taaaataagt tttattttat
     1081 aaaaattgct gattcagtat aattaatatt tacggggtga cataacgtat gaaaaaatca
     1141 gaggattatt cctcctaaat ataaagattt aaaatttagg aggaaattat atatgacttt
     1201 taatattatc aaattagaaa attgggatag aaaagaatat tttgaacact attttaacca
     1261 gcaaactacg tatagcatta ctaaagaaat tgatattact ttgtttaaag atatgataaa
     1321 aaagaaagga tatgaaattt atccttcttt gatttatgca attatggaag ttgtaaataa
     1381 aaataaagtg tttagaacag gaattaatag tgagaataaa ttaggttatt gggataagtt
     1441 aaatcctttg tatacagttt ttaataagca aactgaaaaa tttactaaca tttggactga
     1501 atctgataac aacttcactt ctttttataa taattataaa aatgacttgc ttgaatataa
     1561 agataaagaa gaaatgtttc ctaaaaaacc gatacctgaa aacaccatac cgatttcaat
     1621 gattccttgg attgatttta gttcatttaa tttaaacatt ggtaacaata gcaacttttt
     1681 attgcctatt attacgatag gtaaatttta tagtgagaat aataaaattt atataccagt
     1741 tgctttgcag cttcatcatg ctgtatgtga tggttaccat gcttcattat ttatgaatga
     1801 atttcaagat ataattcata aggtagatga ttggatttag tttttagatt ttggaagtga
     1861 atttaatttt atacacgcaa gtgatcataa aatttatgaa cgtatagcaa ccacattttt
     1921 ggttgcatag gttttgattt tgaattaggt cttgaactat gagtggctag agcttgcatg
     1981 ccagatccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
     2041 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
     2101 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
     2161 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
     2221 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
     2281 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
     2341 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
     2401 cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct
     2461 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
     2521 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
     2581 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tgggcccccc
     2641 ctcgaggtcg acggtatcga taagcttgat atcgaattcc tgcagcccgg gggatccact
     2701 agttctagag cggccgccac cgcggtggag ctctagcact ttgccactca ttttttgcgt
     2761 tagcaaaaac ataaagggta tgggatataa tcccatcaag ccggtatatt cagaacagga
     2821 acagcaacaa gaacaacaaa agaatcaaaa acgagataga ggtatgcact tatagaacat
     2881 gcatttatgc cgagaaaact tattggttgg aatgggctat gtgttagcta acttgttagc
     2941 gagttggttg gacttgaatt gggattaatc ccaagaaagt accaactcaa caacacataa
     3001 agccctgtag gttccgacca ataaggaaat tggaataaag caataaaagg agttgaagaa
     3061 atgaaattca gagaagcctt tgagaatttt ataacaagta agtatgtact tggtgtttta
     3121 gtagttttaa ctgtttacca gataatacaa atgcttaaat aaaaaaagac ttgatctgat
     3181 tagaccaagt cttttgatag tgttatatta ataacaaaat aaaaaggagt cgctcacgcc
     3241 ttgaccaaag tttgtgaacg acatcattca aagaaaaaaa cacc
//
