LOCUS       40924_21878        4157 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Plant expression vector pGIGI, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4157)
  AUTHORS   Ferrando A, Koncz-Kalman Z, Farras R, Tiburcio A, Schell J, Koncz C.
  TITLE     Detection of in vivo protein interactions between Snf1-related 
            kinase subunits with intron-tagged epitope-labelling in plants cells
  JOURNAL   Nucleic Acids Res. 29 (17), 3685-3693 (2001)
  PUBMED    11522840
REFERENCE   2  (bases 1 to 4157)
  AUTHORS   Berendzen KW, Koncz C.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2004) Development, Max-Planck Institute for Plant 
            Breeding Research, Carl-von-Linne-Weg 10, Cologne D-50829, Germany
REFERENCE   3  (bases 1 to 4157)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4157)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2001"; volume: "29"; issue: "17"; pages: 
            "3685-3693"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (12-AUG-2004) Development, Max-Planck Institute for Plant Breeding 
            Research, Carl-von-Linne-Weg 10, Cologne D-50829, Germany"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4157
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..18
                     /note="Tth111I, NotI, XhoI, KpnI; MCS promoter"
     promoter        409..754
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     misc_feature    759..793
                     /note="HindIII, NcoI, SmaI, XbaI, BglII; MCS upstream"
     misc_feature    794..808
                     /label=N-term c-myc epitope
                     /note="N-term c-myc epitope"
     intron          809..997
                     /label=IV2 intron
                     /note="modified second intron of the potato ST-LS1 gene 
                     (Vancanneyt et al., 1990)"
     misc_feature    998..1012
                     /label=C-term c-myc epitope
                     /note="C-term c-myc epitope"
     misc_feature    1022..1041
                     /note="SalI, BamHI, PstI, EcoRI; MCS downstream"
     regulatory      1042..1294
                     /note="polyadenylation signal from nopaline synthase gene
                     of the Agrobacterium Ti plasmid; NOS terminator"
                     /regulatory_class="terminator"
     promoter        complement(1326..1344)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1374..1390)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(1398..1414)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1422..1452)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(1467..1488)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(1776..2364)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2538..3395)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(3396..3500)
                     /label=AmpR promoter
     rep_origin      3527..3982
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     4123..4139
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gcggccgctc gaggtacccc tactccaaaa atgtcaaaga tacagtctca ntaagnacca
       61 aagggctatt gagacttttc aacaaagggt aatttcggga aacctcctcg gattccattg
      121 cccagctatc tgtcacttca tcgaaaggac agtagaaaag gaaggtggct cctacaaatg
      181 ccatcattgc gataaaggaa aggctatcat tcaagatgcc tctgccgaca gtggtcccaa
      241 agatggaccc ccacccacga ggagcatcgt ggaaaaagaa gacgttccaa ccacgtcttc
      301 aaagcaagtg gattgatgtg acatctccac tgacgtaagg gatgacgcac aatcccaccc
      361 ctactccaaa aatgtcaaag atacagtctc agaagaccaa agggctattg agacttttca
      421 acaaagggta atttcgggaa acctcctcgg attccattgc ccagctatct gtcacttcat
      481 cgaaaggaca gtagaaaagg aaggtggctc ctacaaatgc catcattgcg ataaaggaaa
      541 ggctatcatt caagatgcct ctgccgacag tggtcccaaa gatggacccc cacccacgag
      601 gagcatcgtg gaaaaagaag acgttccaac cacgtcttca aagcaagtgg attgatgtga
      661 catctccact gacgtaaggg atgacgcaca atcccactat ccttcgcaag acccttcctc
      721 tatataagga agttcatttc atttggagag gacagcccaa gcttccacca tggcgcccgg
      781 gtctagaaga tctgagcaaa agttgattgt aagtttctgc ttctaccttt gatatatata
      841 taataattat cattaattag tagtaatata atattttaaa tatttttttc aaaataaaag
      901 aatgtagtat atagcaattg cttttctgta gtttataagt gtgtatattt caatttataa
      961 cttttctaat atatgaccaa aatttgttga tgtgcagtct gaggaggatc ttgtcgatcg
     1021 aggatccact gcagtgaatt ctcgttcaaa catttggcaa taaagtttct taagattgaa
     1081 tcctgttgcc ggtcttgcga tgattatcat ataatttctg ttgaattacg ttaagcatgt
     1141 aataattaac atgtaatgca tgacgttatt tatgagatgg gtttttatga ttagagtccc
     1201 gcaattatac atttaatacg cgatagaaaa caaaatatag cgcgcaaact aggataaatt
     1261 atcgcgcgcg gtgtcatctg tcgagggggg gcccggtacc gagctcgcgg ccgctcgacg
     1321 agctccctat agtgagtcgt attanaggcc gacttggcca aattcgtaat catgtcatag
     1381 ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc
     1441 ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc
     1501 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa
     1561 cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg
     1621 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
     1681 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
     1741 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
     1801 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
     1861 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
     1921 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
     1981 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
     2041 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
     2101 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
     2161 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca
     2221 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
     2281 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
     2341 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
     2401 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
     2461 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
     2521 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
     2581 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
     2641 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat
     2701 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
     2761 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
     2821 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt
     2881 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
     2941 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
     3001 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
     3061 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
     3121 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga
     3181 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
     3241 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
     3301 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
     3361 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga
     3421 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
     3481 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgc gccctgtagc
     3541 ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc
     3601 gccctagggc ccggtccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt
     3661 ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac
     3721 ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag
     3781 acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa
     3841 actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg
     3901 atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac
     3961 aaaatattaa cgcttacaat ttccattcgc cattcaggct gcgcaactgt tgggaagggc
     4021 gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt gctgcaaggc
     4081 gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg acggccagtg
     4141 ccaagctgac ttggtca
//
