LOCUS       62056_11565        5494 bp DNA     circular SYN 03-JUL-2019
DEFINITION  Cloning vector pGHKW4, complete sequence.
ACCESSION   MK562404
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5494)
  AUTHORS   Chenyan H.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-FEB-2019) Fujian Agriculture and Forestry University, 
            College of Forestry, No. 15 Shangdian Road, Cangshan District, 
            Fuzhou, Fujian 350002, China
REFERENCE   2  (bases 1 to 5494)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (21-FEB-2019) Fujian Agriculture and Forestry University, College of
            Forestry, No. 15 Shangdian Road, Cangshan District, Fuzhou, Fujian 
            350002, China"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5494
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     regulatory      414..778
                     /note="nptII; promoter of transference"
                     /regulatory_class="promoter"
     CDS             824..1537
                     /label=GFP
                     /note="Aequorea victoria green fluorescent protein"
     CDS             2469..3248
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     primer_bind     complement(3273..3289)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(3297..3313)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3321..3351)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(3366..3387)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(3675..4263)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4437..5294)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(5295..5399)
                     /label=AmpR promoter
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt cgagctctct agagtcaggc
      421 tgttacagct cagagaaagc ttcacgctgc cgcaagcact cagggcgcaa gggctgctaa
      481 aggaagcgga acacgtagaa agccagtccg cagaaacggt gctgaccccg gatgaatgtc
      541 agctactggg ctatctggac aagggaaaac gcaagcgcaa agagaaagca ggtagcttgc
      601 agtgggctta catggcgata gctagactgg gcggttttat ggacagcaag cgaaccggaa
      661 ttgccagctg gggcgccctc tggtaaggtt gggaagccct gcaaagtaaa ctggatggct
      721 ttcttgccgc caaggatctg atggcgcagg ggatcaagat ctgatcaaga gacaggatgg
      781 aattcctgat taactttata aggaggaaaa atccatggct agcatgagta aaggagaaga
      841 acttttcact ggagttgtcc caattcttgt tgaattagat ggtgatgtta atgggcacaa
      901 attttctgtc agtggagagg gtgaaggtga tgcaacatac ggaaaactta cccttaaatt
      961 tatttgcact actggaaaac tacctgttcc atggccaaca cttgtcacta ctttctctta
     1021 tggtgttcaa tgcttttcaa gatacccaga tcatatgaaa cggcatgact ttttcaagag
     1081 tgccatgccc gaaggttatg tacaggaaag aactatattt ttcaaagatg acgggaacta
     1141 caagacacgt gctgaagtca agtttgaagg tgataccctt gttaatagaa tcgagttaaa
     1201 aggtattgat tttaaagaag atggaaacat tcttggacac aaattggaat acaactataa
     1261 ctcacacaat gtatacatca tggcagacaa acaaaagaat ggaatcaaag ttaacttcaa
     1321 aattagacac aacattgaag atggaagcgt tcaactagca gaccattatc aacaaaatac
     1381 tccaattggc gatggccctg tccttttacc agacaaccat tacctgtcca cacaatctgc
     1441 cctttcgaaa gatcccaacg aaaagagaga ccacatggtc cttcttgagt ttgtaacagc
     1501 tgctgggatt acacatggca tggatgaact atacaaaccc gggggtgcgc cggtgccgta
     1561 tccggatccg ctggaaccga tggcattttc agtcaattat gacagcagtt tcggcagcta
     1621 cagcatccat aactatctga acgagtgggc gtcgtcgttt ggcgatatca accacaccaa
     1681 cggtaatgtc accgactcca acagcggcgg tttctacggc ggcagcctgt ccggcagcca
     1741 atatgccgtg accagcaccg ccaacaacat cacctcgttc gtggcgggcg gcaacctgac
     1801 ctacaccctg ttcaacgcac cggcgcacac cctgtacggt caactggact ccctgtcgtt
     1861 cggcgacggc ctgaacggcg gcggctctac accggacaac atccaggttc ctgacgtcag
     1921 cttcggcggc ctgaatctca gcagcctgca ggcgcagggc cacgatggcg tggtacacca
     1981 ggtggtgtac ggcctgatgt ccggcgatac cggcgcgctg gaaaccgcgc tgaacggcat
     2041 cctcgacgac tacggtctga gcgtcaactc caccttcgat caggtggcgg cggcgacggc
     2101 ggtgggcgtg cagcacgccg acagcccgga actgctggcg gcctgaacta gtgcatgcga
     2161 agcggaacac gtagaaagcc agtccgcaga aacggtgctg accccggatg aatgtcagct
     2221 actgggctat ctggacaagg gaaaacgcaa gcgcaaagag aaagcaggta gcttgcagtg
     2281 ggcttacatg gcgatagcta gactgggcgg ttttatggac agcaagcgaa ccggaattgc
     2341 cagctggggc gccctctggt aaggttggga agccctgcaa agtaaactgg atggctttct
     2401 tgccgccaag gatctgatgg cgcaggggat caagatctga tcaagagaca ggatgaggat
     2461 cgtttcgcat gattgaacaa gatggattgc acgcaggttc tccggccgct tgggtggaga
     2521 ggctattcgg ctatgactgg gcacaacaga caatcggctg ctctgatgcc gccgtgttcc
     2581 ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga
     2641 atgaactgca ggacgaggca gcgcggctat cgtggctggc cacgacgggc gttccttgcg
     2701 cagctgtgct cgacgttgtc actgaagcgg gaagggactg gctgctattg ggcgaagtgc
     2761 cggggcagga tctcctgtca tcccaccttg ctcctgccga gaaagtatcc atcatggctg
     2821 atgcaatgcg gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga
     2881 aacatcgcat cgagcgagca cgtactcgga tggaagccgg tcttgtcgat caggatgatc
     2941 tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc aaggcgcgca
     3001 tgcccgacgg cgaggatctc gtcgtgaccc acggcgatgc ctgcttgccg aatatcatgg
     3061 tggaaaatgg ccgcttttct ggattcatcg actgtggccg gctgggtgtg gcggaccgct
     3121 atcaggacat agcgttggct acccgtgata ttgctgaaga gcttggcggc gaatgggctg
     3181 accgcttcct cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc gccttctatc
     3241 gccttctgac gagtaagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt
     3301 tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt
     3361 gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg
     3421 ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg
     3481 cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg
     3541 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
     3601 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
     3661 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
     3721 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
     3781 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
     3841 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
     3901 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
     3961 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
     4021 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
     4081 ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg
     4141 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
     4201 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
     4261 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
     4321 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa
     4381 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa
     4441 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc
     4501 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct
     4561 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca
     4621 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt
     4681 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt
     4741 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc
     4801 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc
     4861 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt
     4921 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact
     4981 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc
     5041 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt
     5101 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg
     5161 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct
     5221 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa
     5281 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt
     5341 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc
     5401 acatttcccc gaaaagtgcc acctgacgtc taagaaacca ttattatcat gacattaacc
     5461 tataaaaata ggcgtatcac gaggcccttt cgtc
//
