LOCUS       V013170                 4429 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V013170
VERSION     V013170
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 4429)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..4429
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(3..458)
                     /direction=LEFT
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     600..616
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        626..644
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    710..734
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             749..771
                     /label="RBS"
                     /note="efficient ribosome binding site from bacteriophage
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             779..1768
                     /gene="rpoA"
                     /label="DNA-directed RNA polymerase subunit alpha"
                     /note="DNA-directed RNA polymerase subunit alpha from
                     Porphyromonas gingivalis (strain ATCC BAA-308 / W83).
                     Accession#: Q7MTP0"
     promoter        complement(2241..2259)
                     /label="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(2280..2296)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(2304..2320)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2328..2358)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(2373..2394)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(2682..3270)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(3444..4301)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(4302..4406)
                     /label="AmpR promoter"
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tgggtacggc
      661 cgtcaaggcc aagcttcccg ggatgtcgac taatacgact cactataggg gaattgtgag
      721 cggataacaa ttcccctcta gaaataattt tgtttaactt taagaaggag atatacatat
      781 ggctatcctg gctttccaga aaccggaaaa cgttctgatg atggaaacct ctgactctat
      841 cgctaaattc gaattcaaac cgctggaacc gggttacggt atcaccatcg gtaacgctct
      901 gcgtcgtatc ctgctgtctt ctctggaagg tttcgctatc accgctatca aaatcgaagg
      961 tgttgaacac gaattcgcta ccatcccggg tgttctggaa gacgttacca acatcatcct
     1021 gaacctgaaa caggttcgtt tcaaacagat cgttccgaac gctgacgttg aaaaagctac
     1081 catcgttatc tctaactctg aagttttccg tgctggtgac ctgaacgctc agctgtctaa
     1141 cttcgaagtt ctgaactcta acctggttat ctgccacctg gacaaatctg ctaccctgac
     1201 catggaattc tctatcaaca aaggtcgtgg ttacgtttct gctgaagaaa accgtgctga
     1261 acacaacgaa ctgtctacca tcgctatcga ctctatctac accccgatcc gtaacgttaa
     1321 atacgctgtt gaaaacttcc gtgttgaaca gaaaaccgac tacgaaaaac tgctgatgga
     1381 agttaccacc gacggttcta tccgtccggt tgacgctctg cgtgaagctg ctcagatcct
     1441 gatctctcac ttctctctgt tcgctgaaaa caaaatcgct atcgaatacg ttgacatcgt
     1501 tgacaccgac gaattcgacg aagactctct gcacatgcgt cagctgctga aatctaaact
     1561 gtctggtctg gacctgtctg ttcgtgctct gaactgcctg aacgctgctg gtgttgacac
     1621 cctgggtgac ctggtttctc tgtctcgttc tgacctgatg aaaatccgta acttcggtaa
     1681 aaaatctctg accgaactgg acgaactgct ggctaccctg aacctgtctt tcggtatgga
     1741 catctctaaa tacaaactgg acaaagactg agcggccgct aatacgactc actatagggg
     1801 aattgtgagc ggataacaat tcccctctag aaataatttt gtttaacttt aagaaggaga
     1861 tatacatatg gaactgaaaa aaatgaacgt tccgatggac accatcaccc gtgacatggt
     1921 tcgtctgtct gaagacaccg aaaacgttta cgaaaccgtt atgatcatcg ctaaacgtgc
     1981 taaccagatc ggtcagcaga tgaaacagga cctggaaaaa aaactgcagg acttctcttc
     2041 ttctaacgac aacctggaag aagttttcga aaaccgtgaa cagatcgaaa tctctcgtta
     2101 ctacgaacac ctgccgaaac cgggtctgat cgctaccgct gaatacgaac aggacaaact
     2161 gtaccaccgt atgccgggtg ctacctctac caacgactga ctcgagatca cgtgaagctt
     2221 gcaagctcca gcttttgttc cctttagtga gggttaattg cgcgcttggc gtaatcatgg
     2281 tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc
     2341 ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg
     2401 ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc
     2461 ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact
     2521 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
     2581 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
     2641 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
     2701 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
     2761 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
     2821 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
     2881 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
     2941 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
     3001 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
     3061 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
     3121 aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
     3181 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
     3241 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
     3301 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
     3361 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
     3421 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
     3481 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
     3541 gagggcttac catctggccc cagtgctgca atgataccgc gagatccacg ctcaccggct
     3601 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
     3661 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
     3721 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg
     3781 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
     3841 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
     3901 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
     3961 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
     4021 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat
     4081 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
     4141 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
     4201 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
     4261 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat
     4321 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
     4381 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccac
//
