LOCUS       V013198                 3975 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V013198
VERSION     V013198
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 3975)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..3975
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(3..458)
                     /direction=LEFT
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     600..616
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        626..644
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             696..1739
                     /gene="ADH2"
                     /label="Alcohol dehydrogenase 2"
                     /note="Alcohol dehydrogenase 2 from Saccharomyces
                     cerevisiae (strain ATCC 204508 / S288c). Accession#:
                     P00331"
     promoter        complement(1787..1805)
                     /label="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(1826..1842)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(1850..1866)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1874..1904)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(1919..1940)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(2228..2816)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(2990..3847)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(3848..3952)
                     /label="AmpR promoter"
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tgggtacggc
      661 cgtcaaggcc aagcttcccg ggatcttatc tagacatgtc aattccagaa actcaaaagg
      721 ctattatttt ctacgaatca aacggtaagc ttgaacacaa ggacattcca gttccaaagc
      781 caaagccaaa cgaacttctt attaacgtta agtactcagg tgtttgtcac actgaccttc
      841 acgcttggca cggtgactgg ccacttccaa ctaagcttcc acttgttggt ggtcacgaag
      901 gtgctggtgt tgttgttggt atgggtgaaa acgttaaggg ttggaagatt ggtgactacg
      961 ctggtattaa gtggcttaac ggttcatgta tggcttgtga atactgtgaa cttggtaacg
     1021 aatcaaactg tccacacgct gacctttcag gttacactca cgacggttca ttccaagaat
     1081 acgctactgc tgacgctgtt caagctgctc acattccaca aggtactgac cttgctgaag
     1141 ttgctccaat tctttgtgct ggtattactg tttacaaggc tcttaagtca gctaaccttc
     1201 gtgctggtca ctgggctgct atttcaggtg ctgctggtgg tcttggttca cttgctgttc
     1261 aatacgctaa ggctatgggt taccgtgttc ttggtattga cggtggtcca ggtaaggaag
     1321 aacttttcac ttcacttggt ggtgaagttt tcattgactt cactaaggaa aaggacattg
     1381 tttcagctgt tgttaaggct actaacggtg gtgctcacgg tattattaac gtttcagttt
     1441 cagaagctgc tattgaagct tcaactcgtt actgtcgtgc taacggtact gttgttcttg
     1501 ttggtcttcc agctggtgct aagtgttcat cagacgtttt caaccacgtt gttaagtcaa
     1561 tttcaattgt tggttcatac gttggtaacc gtgctgacac tcgtgaagct cttgacttct
     1621 tcgctcgtgg tcttgttaag tcaccaatta aggttgttgg tctttcatca cttccagaaa
     1681 tttacgaaaa gatggaaaag ggtcaaattg ctggtcgtta cgttgttgac acttcaaagt
     1741 aaggtaccta agatcacgtg aagcttgcaa gctccagctt ttgttccctt tagtgagggt
     1801 taattgcgcg cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc
     1861 tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat
     1921 gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc
     1981 tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg
     2041 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
     2101 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
     2161 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
     2221 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
     2281 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
     2341 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
     2401 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
     2461 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
     2521 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
     2581 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
     2641 ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc
     2701 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
     2761 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
     2821 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
     2881 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
     2941 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
     3001 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
     3061 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
     3121 taccgcgaga tccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
     3181 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
     3241 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg
     3301 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
     3361 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
     3421 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
     3481 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
     3541 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
     3601 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
     3661 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
     3721 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
     3781 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
     3841 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
     3901 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
     3961 cccgaaaagt gccac
//
