LOCUS       V016057                 7294 bp    DNA     circular SYN 24-FEB-2021
DEFINITION  Exported.
ACCESSION   V016057
VERSION     V016057
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 7294)
  AUTHORS   Grogan CL, Lampe DJ.
  TITLE     Novel Asaia sp. type II secretion signals for Plasmodium inhibition
            in Anopheles stephensi
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7294)
  AUTHORS   Grogan CL, Lampe DJ.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-OCT-2020) Biological Sciences, Duquesne University,
            600 Forbes Ave, Pittsburgh, PA 15219-3016, USA
REFERENCE   3  (bases 1 to 7294)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (18-OCT-2020) Biological Sciences, Duquesne University, 600 Forbes
            Ave, Pittsburgh, PA 15219-3016, USA"
FEATURES             Location/Qualifiers
     source          1..7294
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             458..1249
                     /label="NeoR/KanR"
                     /note="aminoglycoside phosphotransferase"
     regulatory      1552..1763
                     /label="PnptII"
                     /note="PnptII"
                     /regulatory_class="promoter"
     misc_feature    1767..1892
                     /label="gamma-glutamyltranspeptidase secretion signal"
                     /note="gamma-glutamyltranspeptidase secretion signal"
     CDS             1869..3248
                     /gene="phoA"
                     /label="Alkaline phosphatase"
                     /note="Alkaline phosphatase from Escherichia coli (strain
                     K12). Accession#: P00634"
     CDS             complement(4843..5502)
                     /label="pBBR1 Rep"
                     /note="replication protein for the broad-host-range plasmid
                     pBBR1 from Bordetella bronchiseptica"
     rep_origin      complement(5503..6272)
                     /direction=LEFT
                     /label="pBBR1 oriV"
                     /note="replication origin of the broad-host-range plasmid
                     pBBR1 from Bordetella bronchiseptica; requires the pBBR1
                     Rep protein for replication"
ORIGIN
        1 accttcggga gcgcctgaag cccgttctgg acgccctggg gccgttgaat cgggatatgc
       61 aggccaaggc cgccgcgatc atcaaggccg tgggcgaaaa gctgctgacg gaacagcggg
      121 aagtccagcg ccagaaacag gcccagcgcc agcaggaacg cgggcgcgca catttccccg
      181 aaaagtgcca cctgggatga atgtcagcta ctgggctatc tggacaaggg aaaacgcaag
      241 cgcaaagaga aagcaggtag cttgcagtgg gcttacatgg cgatagctag actgggcggt
      301 tttatggaca gcaagcgaac cggaattgcc agctggggcg ccctctggta aggttgggaa
      361 gccctgcaaa gtaaactgga tggctttctt gccgccaagg atctgatggc gcaggggatc
      421 aagatctgat caagagacag gatgaggatc gtttcgcatg attgaacaag atggattgca
      481 cgcaggttct ccggccgctt gggtggagag gctattcggc tatgactggg cacaacagac
      541 aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc cggttctttt
      601 tgtcaagacc gacctgtccg gtgccctgaa tgaactgcag gacgaggcag cgcggctatc
      661 gtggctggcc acgacgggcg ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg
      721 aagggactgg ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc
      781 tcctgccgag aaagtatcca tcatggctga tgcaatgcgg cggctgcata cgcttgatcc
      841 ggctacctgc ccattcgacc accaagcgaa acatcgcatc gagcgagcac gtactcggat
      901 ggaagccggt cttgtcgatc aggatgatct ggacgaagag catcaggggc tcgcgccagc
      961 cgaactgttc gccaggctca aggcgcgcat gcccgacggc gaggatctcg tcgtgaccca
     1021 tggcgatgcc tgcttgccga atatcatggt ggaaaatggc cgcttttctg gattcatcga
     1081 ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata gcgttggcta cccgtgatat
     1141 tgctgaagag cttggcggcg aatgggctga ccgcttcctc gtgctttacg gtatcgccgc
     1201 tcccgattcg cagcgcatcg ccttctatcg ccttcttgac gagttcttct gagcgggact
     1261 ctggggttcg aaatgaccga ccaagcgacg cccaacctgc catcacgaga tttcgattcc
     1321 accgccgcct tctatgaaag gttgggcttc ggaatcgttt tccgggacgc cggctggatg
     1381 atcctccagc gcggggatct catgctggag ttcttcgccc acccccatgg gcaaatatta
     1441 tacgcaaggc gacaaggtgc tgatgccgct ggcgattcag gttcatcatg ccgtttgtga
     1501 tggcttccat gtcggcagaa tgcttaatga attacaacag tttttatgca taaccggaat
     1561 tgccagctgg ggcgccctct ggtaaggttg ggaagccctg caaagtaaac tggatggctt
     1621 tcttgccgcc aaggatctga tggcgcaggg gatcaagatc tgatcaagag acaggatgtc
     1681 gactctagag gatccccggg taccgagctc gaattcggct tattccctaa ctaactaaag
     1741 attaacttta taaggaggaa aaacatatga agcgtgtctg cacccgggtt gcggccctgc
     1801 cgctcctcct cgccagcatt tctgtctcga gtctcgccca ggctgcgcca gcggccatgc
     1861 cctatgaccc gctggccttc ggcacggtca acttaattaa aacctgcagg atgcctgttc
     1921 tggaaaaccg ggctgctcag ggcgatatta ctgcacccgg cggtgctcgc cgtttaacgg
     1981 gtgatcagac tgccgctctg cgtgattctc ttagcgataa acctgcaaaa aatattattt
     2041 tgctgattgg cgatgggatg ggggactcgg aaattactgc cgcacgtaat tatgccgaag
     2101 gtgcgggcgg cttttttaaa ggtatagatg ccttaccgct taccgggcaa tacactcact
     2161 atgcgctgaa taaaaaaacc ggcaaaccgg actacgtcac cgactcggct gcatcagcaa
     2221 ccgcctggtc aaccggtgtc aaaacctata acggcgcgct gggcgtcgat attcacgaaa
     2281 aagatcaccc aacgattctg gaaatggcaa aagccgcagg tctggcgacc ggtaacgttt
     2341 ctaccgcaga gttgcaggat gccacgcccg ctgcgctggt ggcacatgtg acctcgcgca
     2401 aatgctacgg tccgagcgcg accagtgaaa aatgtccggg taacgctctg gaaaaaggcg
     2461 gaaaaggatc gattaccgaa cagctgctta acgctcgtgc cgacgttacg cttggcggcg
     2521 gcgcaaaaac ctttgctgaa acggcaaccg ctggtgaatg gcagggaaaa acgctgcgtg
     2581 aacaggcaca ggcgcgtggt tatcagttgg tgagcgatgc tgcctcactg aattcggtga
     2641 cggaagcgaa tcagcaaaaa cccctgcttg gcctgtttgc tgacggcaat atgccagtgc
     2701 gctggctagg accgaaagca acgtaccatg gcaatatcga taagcccgca gtcacctgta
     2761 cgccaaatcc gcaacgtaat gacagtgtac caaccctggc gcagatgacc gacaaagcca
     2821 ttgaattgtt gagtaaaaat gagaaaggct ttttcctgca agttgaaggt gcgtcaatcg
     2881 ataaacagga tcatgctgcg aatccttgtg ggcaaattgg cgagacggtc gatctcgatg
     2941 aagccgtaca acgggcgctg gaattcgcta aaaaggaggg taacacgctg gtcatagtca
     3001 ccgctgatca cgcccacgcc agccagattg ttgcgccgga taccaaagct ccgggcctca
     3061 cccaggcgct aaataccaaa gatggcgcag tgatggtgat gagttacggg aactccgaag
     3121 aggattcaca agaacatacc ggcagtcagt tgcgtattgc ggcgtatggc ccgcatgccg
     3181 ccaatgttgt tggactgacc gaccagaccg atctcttcta caccatgaaa gccgctctgg
     3241 ggctgaaata aaaccgcgcc cggcagtgaa ttttcgctgc cgggtggttt ttttgctgtt
     3301 agcaaccaga cttaatggca gatcacgggc gcatacgctc atggttaaaa catgaagagg
     3361 gatggtgcta tgaaaataac attactggtt accttgcttt tcggtctggt ttttttaacc
     3421 accgtcggcg ctgccgagag aactttaacc ccacaacaac agcgtatgac ctcctgtaat
     3481 cagcaggcga cggcgcaggc gttgaaaggg gatgctcgta agacctacat gagtgattgc
     3541 ctgaagaaca gcaagtctgc gcctggcgaa aaaagtttga cgccacagca gcaaaagatg
     3601 cgcgaatgca ataatcaagc aacacaacaa tctctgaaag gtgatgatcg taataagttt
     3661 atgagtgcct gcctcaagaa agccgcctga tacctgatag tgctaacggg tgagctacga
     3721 aaatggctca cccgaaatat catacttctg cctttagctc cgtctctata atttgggaaa
     3781 attgtttctg aatgttccca aaaataatga atgatgaaaa ctttttcaaa aaagcggcgg
     3841 cgcacgggga ggaacctcct ttaactcctc aaaacgaaca tcagcggtcc gggctgcgct
     3901 tcgcccgtcg cgtcagacta ccccgtgcgg ttggcctggc tggcatgttc ttaccgattg
     3961 cttcaacgct ggtttcacac ccgccgccgg gctggtggtg gctggtgttg gtcggctggg
     4021 cgttcgtctg gccgcattta gcctggcaga tagcgagcag ggccgtcgat ccgcttagcc
     4081 gggaaattta caacttaaaa accgatgcag tattagcggg aatgtgggta ggcgtaatgg
     4141 gcgtaaacgt gctgccttcc accgcgatgt tgatgattat gtgtctgaat ttgatggggg
     4201 caggcggccc ccgtctgttt gtcgcgggtc tggtgttgat ggtggtttcc tgccttgtca
     4261 ccggccggcc ggatccgaat tcgcgttaat attttgttaa aattcgcgtt aaatttttgt
     4321 taaatcagct cattttttaa ccaataggcc gactgcgatg agtggcaggg cggggcgtaa
     4381 tttttttaag gcagttattg gtgcccttaa acgcctggtg ctacgcctga ataagtgata
     4441 ataagcggat gaatggcaga aattcgaaag caaattcgac ccggtcgtcg gttcagggca
     4501 gggtcgttaa atagccgctt atgtctattg ctggtttacc ggtttattga ctaccggaag
     4561 cagtgtgacc gtgtgcttct caaatgcctg aggccagttt gctcaggctc tccccgtgga
     4621 ggtaataatt gacgatatga tcatttattc tgcctcccag agcctgataa aaacggtgaa
     4681 tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg
     4741 cgacgcaacg cggggaggca gacaaggtat agggcggcga ggcggctaca gccgatagtc
     4801 tggaacagcg cacttacggg ttgctgcgca acccaagtgc taccggcgcg gcagcgtgac
     4861 ccgtgtcggc ggctccaacg gctcgccatc gtccagaaaa cacggctcat cgggcatcgg
     4921 caggcgctgc tgcccgcgcc gttcccattc ctccgtttcg gtcaaggctg gcaggtctgg
     4981 ttccatgccc ggaatgccgg gctggctggg cggctcctcg ccggggccgg tcggtagttg
     5041 ctgctcgccc ggatacaggg tcgggatgcg gcgcaggtcg ccatgcccca acagcgattc
     5101 gtcctggtcg tcgtgatcaa ccaccacggc ggcactgaac accgacaggc gcaactggtc
     5161 gcggggctgg ccccacgcca cgcggtcatt gaccacgtag gccgacacgg tgccggggcc
     5221 gttgagcttc acgacggaga tccagcgctc ggccaccaag tccttgactg cgtattggac
     5281 cgtccgcaaa gaacgtccga tgagcttgga aagtgtcttc tggctgacca ccacggcgtt
     5341 ctggtggccc atctgcgcca cgaggtgatg cagcagcatt gccgccgtgg gtttcctcgc
     5401 aataagcccg gcccacgcct catgcgcttt gcgttccgtt tgcacccagt gaccgggctt
     5461 gttcttggct tgaatgccga tttctctgga ctgcgtggcc atgcttatct ccatgcggta
     5521 gggtgccgca cggttgcggc accatgcgca atcagctgca acttttcggc agcgcgacaa
     5581 caattatgcg ttgcgtaaaa gtggcagtca attacagatt ttctttaacc tacgcaatga
     5641 gctattgcgg ggggtgccgc aatgagctgt tgcgtacccc ccttttttaa gttgttgatt
     5701 tttaagtctt tcgcatttcg ccctatatct agttctttgg tgcccaaaga agggcacccc
     5761 tgcggggttc ccccacgcct tcggcgcggc tccccctccg gcaaaaagtg gcccctccgg
     5821 ggcttgttga tcgactgcgc ggccttcggc cttgcccaag gtggcgctgc ccccttggaa
     5881 cccccgcact cgccgccgtg aggctcgggg ggcaggcggg cgggcttcgc cttcgactgc
     5941 ccccactcgc ataggcttgg gtcgttccag gcgcgtcaag gccaagccgc tgcgcggtcg
     6001 ctgcgcgagc cttgacccgc cttccacttg gtgtccaacc ggcaagcgaa gcgcgcaggc
     6061 cgcaggccgg aggcttttcc ccagagaaaa ttaaaaaaat tgatggggca aggccgcagg
     6121 ccgcgcagtt ggagccggtg ggtatgtggt cgaaggctgg gtagccggtg ggcaatccct
     6181 gtggtcaagc tcgtgggcag gcgcagcctg tccatcagct tgtccagcag ggttgtccac
     6241 gggccgagcg aagcgagcca gccggtggcc gctcgcggcc atcgtccaca tatccacggg
     6301 ctggcaaggg agcgcagcga ccgcgcaggg cgaagcccgg agagcaagcc cgtagggcgc
     6361 cgcagccgcc gtaggcggtc acgactttgc gaagcaaagt ctagtgagta tactcaagca
     6421 ttgagtggcc cgccggaggc accgccttgc gctgcccccg tcgagccggt tggacaccaa
     6481 aagggagggg caggcatggc ggcatacgcg atcatgcgat gcaagaagct ggcgaaaatg
     6541 ggcaacgtgg cggccagtct caagcacgcc taccgcgagc gcgagacgcc caacgctgac
     6601 gccagcagga cgccagagaa cgagcactgg gcggccagca gcaccgatga agcgatgggc
     6661 cgactgcgcg agttgctgcc agagaagcgg cgcaaggacg ctgtgttggc ggtcgagtac
     6721 gtcatgacgg ccagcccgga atggtggaag tcggccagcc aagaacagca ggcggcgttc
     6781 ttcgagaagg cgcacaagtg gctggcggac aagtacgggg cggatcgcat cgtgacggcc
     6841 agcatccacc gtgacgaaac cagcccgcac atgaccgcgt tcgtggtgcc gctgacgcag
     6901 gacggcaggc tgtcggccaa ggagttcatc ggcaacaaag cgcagatgac ccgcgaccag
     6961 accacgtttg cggccgctgt ggccgatcta gggctgcaac ggggcatcga gggcagcaag
     7021 gcacgtcaca cgcgcattca ggcgttctac gaggccctgg agcggccacc agtgggccac
     7081 gtcaccatca gcccgcaagc ggtcgagcca cgcgcctatg caccgcaggg attggccgaa
     7141 aagctgggaa tctcaaagcg cgttgagacg ccggaagccg tggccgaccg gctgacaaaa
     7201 gcggttcggc aggggtatga gcctgcccta caggccgccg caggagcgcg tgagatgcgc
     7261 aagaaggccg atcaagccca agagacggcc cgag
//
