LOCUS       40924_21623        3337 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Vector for expressing GFPuv in bacteria.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3337)
  AUTHORS   Clontech
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 3337)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Translation from the lacZ start codon produces a fusion protein in 
            E. coli.
FEATURES             Location/Qualifiers
     source          1..3337
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    107..128
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        143..173
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    181..197
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     205..221
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             289..1002
                     /codon_start=1
                     /label=GFPuv
                     /note="GFP variant optimized for excitation by UV light"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFSYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKA
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     promoter        1432..1536
                     /label=AmpR promoter
     CDS             1537..2394
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      2568..3156
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
       61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
      121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
      181 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagcttg
      241 catgcctgca ggtcgactct agaggatccc cgggtaccgg tagaaaaaat gagtaaagga
      301 gaagaacttt tcactggagt tgtcccaatt cttgttgaat tagatggtga tgttaatggg
      361 cacaaatttt ctgtcagtgg agagggtgaa ggtgatgcaa catacggaaa acttaccctt
      421 aaatttattt gcactactgg aaaactacct gttccatggc caacacttgt cactactttc
      481 tcttatggtg ttcaatgctt ttcccgttat ccggatcata tgaaacggca tgactttttc
      541 aagagtgcca tgcccgaagg ttatgtacag gaacgcacta tatctttcaa agatgacggg
      601 aactacaaga cgcgtgctga agtcaagttt gaaggtgata cccttgttaa tcgtatcgag
      661 ttaaaaggta ttgattttaa agaagatgga aacattctcg gacacaaact cgagtacaac
      721 tataactcac acaatgtata catcacggca gacaaacaaa agaatggaat caaagctaac
      781 ttcaaaattc gccacaacat tgaagatgga tccgttcaac tagcagacca ttatcaacaa
      841 aatactccaa ttggcgatgg ccctgtcctt ttaccagaca accattacct gtcgacacaa
      901 tctgcccttt cgaaagatcc caacgaaaag cgtgaccaca tggtccttct tgagtttgta
      961 actgctgctg ggattacaca tggcatggat gagctctaca aataatgaat tccaactgag
     1021 cgccggtcgc taccattacc aacttgtctg gtgtcaaaaa taataggcct actagtcggc
     1081 cgtacgggcc ctttcgtctc gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc
     1141 agctcccgga gacggtcaca gcttgtctgt aagcggatgc cgggagcaga caagcccgtc
     1201 agggcgcgtc agcgggtgtt ggcgggtgtc ggggctggct taactatgcg gcatcagagc
     1261 agattgtact gagagtgcac catatgcggt gtgaaatacc gcacagatgc gtaaggagaa
     1321 aataccgcat caggcggcct taagggcctc gtgatacgcc tatttttata ggttaatgtc
     1381 atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc
     1441 cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc
     1501 tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc
     1561 gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg
     1621 gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat
     1681 ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc
     1741 acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa
     1801 ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa
     1861 aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt
     1921 gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct
     1981 tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat
     2041 gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg
     2101 cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg
     2161 atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt
     2221 attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg
     2281 ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg
     2341 gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg
     2401 tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa
     2461 aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt
     2521 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
     2581 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
     2641 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
     2701 ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta
     2761 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
     2821 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
     2881 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
     2941 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
     3001 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
     3061 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
     3121 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
     3181 cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat
     3241 tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg
     3301 accgagcgca gcgagtcagt gagcgaggaa gcggaag
//
