LOCUS       62056_11535        3304 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3304)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..3304
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    107..128
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        143..173
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    181..197
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     205..221
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             304..1017
                     /codon_start=1
                     /label=yeGFP
                     /note="yeast-enhanced green fluorescent protein"
                     /translation="MRKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     promoter        1399..1503
                     /label=AmpR promoter
     CDS             1504..2361
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      2535..3123
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
       61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
      121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
      181 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagcttg
      241 catgcctgca ggtcgactct agaggatccc cgggtaccta gaattaaaga ggagaaatta
      301 agcatgcgta aaggagaaga acttttcact ggagttgtcc caattcttgt tgaattagat
      361 ggtgatgtta atgggcacaa attttctgtc agtggagagg gtgaaggtga tgcaacatac
      421 ggaaaactta cccttaaatt tatttgcact actggaaaac tacctgttcc atggccaaca
      481 cttgtcacta ctttcggtta tggtgttcaa tgctttgcga gatacccaga tcatatgaaa
      541 cagcatgact ttttcaagag tgccatgccc gaaggttatg tacaggaaag aactatattt
      601 ttcaaagatg acgggaacta caagacacgt gctgaagtca agtttgaagg tgataccctt
      661 gttaatagaa tcgagttaaa aggtattgat tttaaagaag atggaaacat tcttggacac
      721 aaattggaat acaactataa ctcacacaat gtatacatca tggcagacaa acaaaagaat
      781 ggaatcaaag ttaacttcaa aattagacac aacattgaag atggaagcgt tcaactagca
      841 gaccattatc aacaaaatac tccaattggc gatggccctg tccttttacc agacaaccat
      901 tacctgtcca cacaatctaa gctttcgaaa gatcccaacg aaaagagaga ccacatggtc
      961 cttcttgagt ttgtaacagc tgctgggatt acacatggca tggatgaact atacaaataa
     1021 gcttaattag ctgacctact agtcggccgt acgggccctt tcgtctcgcg cgtttcggtg
     1081 atgacggtga aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag
     1141 cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg
     1201 gctggcttaa ctatgcggca tcagagcaga ttgtactgag agtgcaccat atgcggtgtg
     1261 aaataccgca cagatgcgta aggagaaaat accgcatcag gcggccttaa gggcctcgtg
     1321 atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc
     1381 acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat
     1441 atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag
     1501 agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt
     1561 cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt
     1621 gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc
     1681 cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta
     1741 tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac
     1801 ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa
     1861 ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg
     1921 atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc
     1981 cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg
     2041 atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta
     2101 gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg
     2161 cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg
     2221 tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc
     2281 tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt
     2341 gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt
     2401 gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc
     2461 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag
     2521 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
     2581 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
     2641 aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag
     2701 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
     2761 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
     2821 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
     2881 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
     2941 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
     3001 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
     3061 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
     3121 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac
     3181 atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga
     3241 gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg
     3301 gaag
//
