LOCUS       V006036                 7390 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V006036
VERSION     V006036
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 7390)
  AUTHORS   Bisicchia P, Botella E, Devine KM.
  TITLE     Suite of novel vectors for ectopic insertion of GFP, CFP and IYFP
            transcriptional fusions in single copy at the amyE and bglS loci in
            Bacillus subtilis
  JOURNAL   Plasmid 64 (3), 143-149 (2010)
   PUBMED   20600285
REFERENCE   2  (bases 1 to 7390)
  AUTHORS   Bisicchia P.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2010) Trinity College Dublin, 13 Meades Terrace,
            Dublin 2, Ireland
REFERENCE   3  (bases 1 to 7390)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7390)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plasmid";
            date: "2010"; volume: "64"; issue: "3"; pages: "143-149"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (06-MAY-2010) Trinity College Dublin, 13 Meades Terrace, Dublin 2,
            Ireland"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7390
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_recomb     115..651
                     /label="amyE front"
                     /note="amyE front"
     terminator      complement(673..766)
                     /label="lambda t0 terminator"
                     /note="transcription terminator from phage lambda"
     CDS             complement(789..1502)
                     /label="yeGFP"
                     /note="yeast-enhanced green fluorescent protein"
     misc_feature    complement(1503..1538)
                     /label="LIC site"
                     /note="LIC site"
     CDS             1691..2338
                     /gene="cat"
                     /label="Chloramphenicol acetyltransferase"
                     /note="Chloramphenicol acetyltransferase from
                     Staphylococcus aureus. Accession#: P00485"
     misc_recomb     2712..3748
                     /label="amyE back"
                     /note="amyE back"
     CDS             4942..5130
                     /label="rop"
                     /note="Rop protein, which maintains plasmids at low copy
                     number"
     misc_feature    5235..5375
                     /label="bom"
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(5561..6149)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(6323..7180)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(7181..7285)
                     /label="AmpR promoter"
ORIGIN
        1 aacaaaattc tccagtcttc acatcggttt gaaaggagga agcggaagaa tgaagtaaga
       61 gggatttttg actccgaagt aagtcttcaa aaaatcaaat aaggagtgtc aagaatgttt
      121 gcaaaacgat tcaaaacctc tttactgccg ttattcgctg gatttttatt gctgtttcat
      181 ttggttctgg caggaccggc ggctgcgagt gctgaaacgg cgaacaaatc gaatgagctt
      241 acagcaccgt cgatcaaaag cggaaccatt cttcatgcat ggaattggtc gttcaatacg
      301 ttaaaacaca atatgaagga tattcatgat gcaggatata cagccattca gacatctccg
      361 attaaccaag taaaggaagg gaatcaagga gataaaagca tgtcgaactg gtactggctg
      421 tatcagccga catcgtatca aattggcaac cgttacttag gtactgaaca agaatttaaa
      481 gaaatgtgtg cagccgctga agaatatggc ataaaggtca ttgttgacgc ggtcatcaat
      541 cataccacca gtgattatgc cgcgatttcc aatgaggtta agagtattcc aaactggaca
      601 catggaaaca cacaaattaa aaactggtct gatcgaaata gtacataatg ggaattcggt
      661 tctgtagtcg acttctcacc aataaaaaac gcccggcggc aaccgagcgt tctgaacaaa
      721 tccagatgga gttctgaggt cattactgga tctatcaaca ggagtccaag ctcagctaat
      781 taagcttatt tgtatagttc atccatgcca tgtgtaatcc cagcagctgt tacaaactca
      841 agaaggacca tgtggtctct cttttcgttg ggatctttcg aaagggcaga ttgtgtggac
      901 aggtaatggt tgtctggtaa aaggacaggg ccatcgccaa ttggagtatt ttgttgataa
      961 tggtctgcta gttgaacgct tccatcttca atgttgtgtc taattttgaa gttaactttg
     1021 attccattct tttgtttgtc tgccatgatg tatacattgt gtgagttata gttgtattcc
     1081 aatttgtgtc caagaatgtt tccatcttct ttaaaatcaa taccttttaa ctcgattcta
     1141 ttaacaaggg tatcaccttc aaacttgact tcagcacgtg tcttgtagtt cccgtcatct
     1201 ttgaaaaata tagttctttc ctgtacataa ccttcgggca tggcactctt gaaaaagtca
     1261 tgccgtttca tatgatctgg gtatctcgca aagcattgaa caccataacc gaaagtagtg
     1321 acaagtgttg gccatggaac aggtagtttt ccagtagtgc aaataaattt aagggtaagt
     1381 tttccgtatg ttgcatcacc ttcaccctct ccactgacag aaaatttgtg cccattaaca
     1441 tcaccatcta attcaacaag aattgggaca actccagtga aaagttcttc tcctttacgc
     1501 atagtagttc ctccttcccg ggaaagcccg cggtaaaaga attcgaaaat tggataaagt
     1561 gggatatttt taaaatatat atttatgtta cagtaatatt gacttttaaa aaaggattga
     1621 ttctaatgaa gaaagcagac aagtaagcct cctaaattca ctttagataa aaatttagga
     1681 ggcatatcaa atgaacttta ataaaattga tttagacaat tggaagagaa aagagatatt
     1741 taatcattat ttgaaccaac aaacgacttt tagtataacc acagaaattg atattagtgt
     1801 tttataccga aacataaaac aagaaggata taaattttac cctgcattta ttttcttagt
     1861 gacaagggtg ataaactcaa atacagcttt tagaactggt tacaatagcg acggagagtt
     1921 aggttattgg gataagttag agccacttta tacaattttt gatggtgtat ctaaaacatt
     1981 ctctggtatt tggactcctg taaagaatga cttcaaagag ttttatgatt tatacctttc
     2041 tgatgtagag aaatataatg gttcggggaa attgtttccc aaaacaccta tacctgaaaa
     2101 tgctttttct ctttctatta ttccatggac ttcatttact gggtttaact taaatatcaa
     2161 taataatagt aattaccttc tacccattat tacagcagga aaattcatta ataaaggtaa
     2221 ttcaatatat ttaccgctat ctttacaggt acatcattct gtttgtgatg gttatcatgc
     2281 aggattgttt atgaactcta ttcaggaatt gtcagatagg cctaatgact ggcttttata
     2341 atatgagata atgccgactg tactttttac agtcggtttt ctaatgtcac taacctgccc
     2401 cgttagttga agaaggtttt tatattacag ctccagatcc tctacgccgg acgcatcgtg
     2461 gccggcatca ccggcgccac aggtgcggtt gctggcgcct atatcgccga catcaccgat
     2521 ggggaagatc gggctcgcca cttcgggctc atgagcgctt gtttcggcgt gggtatggtg
     2581 gcaggccccg tggccggggg actgttgggc gccatctcct tgcatgcacc attccttgcg
     2641 gcggcggtgc tcaacggcct caacctacta ctgggctgct tcctaatgca ggagtcgcat
     2701 aagggagagc gtcgacatgg atgagcgatg atgatatccg tttaggctgg gcggtgatag
     2761 cttctcgttc aggcagtacg cctcttttct tttccagacc tgagggaggc ggaaatggtg
     2821 tgaggttccc cagatctggg ggaaaagcca aataggcgat cgcgggagtg ctttatttga
     2881 agatcaggct atcactgcgg tcaatagatt tcacaatgtg atggctggac agcctgagga
     2941 actctcgaac ccgaatggaa acaaccagat atttatgaat cagcgcggct cacatggcgt
     3001 tgtgctggca aatgcaggtt catcctctgt ctctatcaat acggcaacaa aattgcctga
     3061 tggcaggtat gacaataaag ctggagcggg ttcatttcaa gtgaacgatg gtaaactgac
     3121 aggcacgatc aatgccaggt ctgtagctgt gctttatcct gatgatattg caaaagcgcc
     3181 tcatgttttc cttgagaatt acaaaacagg tgtaacacat tctttcaatg atcaactgac
     3241 gattaccttg cgtgcagatg cgaatacaac aaaagccgtt tatcaaatca ataatggacc
     3301 agacgacagg cgtttaagga tggagatcaa ttcacaatcg gaaaaggaga tccaatttgg
     3361 caaaacatac accatcatgt taaaaggaac gaacagtgat ggtgtaacga ggaccgagaa
     3421 atacagtttt gttaaaagag atccagcgtc ggccaaaacc atcggctatc aaaatccgaa
     3481 tcattggagc caggtaaatg cttatatcta taaacatgat gggagccgag taattgaatt
     3541 gaccggatct tggcctggaa aaccaatgac taaaaatgca gacggaattt acacgctgac
     3601 gctgcctgcg gacacggata caaccaacgc aaaagtgatt tttaataatg gcagcgccca
     3661 agtgcccggt cagaatcagc ctggctttga ttacgtgcta aatggtttat ataatgactc
     3721 gggcttaagc ggttctcttc cccattgagg gcaaggctag acgggactta ccgaaagaaa
     3781 ccatcaatga tggtttcttt tttgttcata aatcagacaa aacttttctc ttgcaaaagt
     3841 ttgtgaagtg ttgcacaata taaatgtgaa atacttcaca aacaaaaaga catcaaagag
     3901 aaacataccc tgcaaggatg ctgatattgt ctgcatttgc gccggagcaa accaaaaacc
     3961 tggtgagaca cgccttgaat ttgacggcat tttcttagtc gcgacgcgag gctggatggc
     4021 cttccccatt atgattcttc tcgcttccgg cggcatcggg atgcccgcgt tgcaggccat
     4081 gctgtccagg caggtagatg acgaccatca gggacagctt caaggatcgc tcgcggctct
     4141 taccagccta acttcgatca ctggaccgct gatcgtcacg gcgatttatg ccgcctcggc
     4201 gagcacatgg aacgggttgg catggattgt aggcgccgcc ctataccttg tctgcctccc
     4261 cgcgttgcgt cgcggtgcat ggagccgggc cacctcgacc tgaatggaag ccggcggcac
     4321 ctcgctaacg gattcaccac tccaagaatt ggagccaatc aattcttgcg gagaactgtg
     4381 aatgcgcaaa ccaacccttg gcagaacata tccatcgcgt ccgccatctc cagcagccgc
     4441 acgcggcgca tctcgggcag cgttgggtcc tggccacggg tgcgcatgat cgtgctcctg
     4501 tcgttgagga cccggctagg ctggcggggt tgccttactg gttagcagaa tgaatcaccg
     4561 atacgcgagc gaacgtgaag cgactgctgc tgcaaaacgt ctgcgacctg agcaacaaca
     4621 tgaatggtct tcggtttccg tgtttcgtaa agtctggaaa cgcggaagtc agcgccctgc
     4681 accattatgt tccggatctg catcgcagga tgctgctggc taccctgtgg aacacctaca
     4741 tctgtattaa cgaagcgctg gcattgaccc tgagtgattt ttctctggtc ccgccgcatc
     4801 cataccgcca gttgtttacc ctcacaacgt tccagtaacc gggcatgttc atcatcagta
     4861 acccgtatcg tgagcatcct ctctcgtttc atcggtatca ttacccccat gaacagaaat
     4921 cccccttaca cggaggcatc agtgaccaaa caggaaaaaa ccgcccttaa catggcccgc
     4981 tttatcagaa gccagacatt aacgcttctg gagaaactca acgagctgga cgcggatgaa
     5041 caggcagaca tctgtgaatc gcttcacgac cacgctgatg agctttaccg cagctgcctc
     5101 gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccgga gacggtcaca
     5161 gcttgtctgt aagcggatgc cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt
     5221 ggcgggtgtc ggggcgcagc catgacccag tcacgtagcg atagcggagt gtatactggc
     5281 ttaactatgc ggcatcagag cagattgtac tgagagtgca ccatatgcgg tgtgaaatac
     5341 cgcacagatg cgtaaggaga aaataccgca tcaggcgctc ttccgcttcc tcgctcactg
     5401 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
     5461 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
     5521 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
     5581 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
     5641 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
     5701 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
     5761 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
     5821 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
     5881 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
     5941 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
     6001 ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
     6061 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
     6121 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
     6181 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
     6241 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
     6301 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
     6361 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
     6421 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc
     6481 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
     6541 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
     6601 cagttaatag tttgcgcaac gttgttgcca ttgctgcagg catcgtggtg tcacgctcgt
     6661 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
     6721 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
     6781 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
     6841 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
     6901 gtatgcggcg accgagttgc tcttgcccgg cgtcaacacg ggataatacc gcgccacata
     6961 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
     7021 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
     7081 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
     7141 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
     7201 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
     7261 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag
     7321 aaaccattat tatcatgaca ttaacctata aaaataggcg tatcacgagg ccctttcgtc
     7381 ttcaagaatt
//
