LOCUS       V006034                10764 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V006034
VERSION     V006034
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 10764)
  AUTHORS   Ding H, Gong S, Tian Y, Yang Z, Brunham R, Zhong G.
  TITLE     Transformation of Sexually Transmitted Infection-Causing Serovars of
            Chlamydia trachomatis Using Blasticidin for Selection
  JOURNAL   PLoS ONE 8 (11), E80534 (2013)
   PUBMED   24303023
REFERENCE   2  (bases 1 to 10764)
  AUTHORS   Zhong G, Gong S, Ding H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-OCT-2013) Microbiology and Immunology, University of
            Texas Health Science Center San Antonio, 7703 Floyd Curl Drive, San
            Antonio, TX 78229, USA
REFERENCE   3  (bases 1 to 10764)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 10764)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "PLoS ONE";
            date: "2013"; volume: "8"; issue: "11"; pages: "E80534"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (01-OCT-2013) Microbiology and Immunology, University of Texas
            Health Science Center San Antonio, 7703 Floyd Curl Drive, San
            Antonio, TX 78229, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..10764
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..88
                     /note="putative; contains 4 copies of a 22bp repeat"
     CDS             165..680
                     /codon_start=1
                     /locus_tag="PSW2_1"
                     /product="putative virulence integrase pGP7-D"
                     /label="PSW2_1"
                     /protein_id="AHD24922.1"
                     /translation="MGSMAFHKSRLFLTFGDASEIWLSTLSYLTRKNYASGINFLVSLE
                     ILDLSETLIKAISLDHSESLFKIKSLDVFNGKVVSEASKQARAACYISFTKFLYRLTKG
                     YIKPAIPLKDFGNTTFFKIRDKIKTESISKQEWTVFFEALRIVNYRDYLIGKLIVQGIP
                     GYRARIHR"
     gene            165..680
                     /locus_tag="PSW2_1"
                     /label="PSW2_1"
     promoter        complement(694..712)
                     /label="SP6 promoter"
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     promoter        1058..1162
                     /label="AmpR promoter"
     CDS             1163..1534
                     /label="BleoR"
                     /note="antibiotic-binding protein"
     rep_origin      1708..2296
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     promoter        2554..2572
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             3100..3804
                     /label="GFP (S65T)"
                     /note="S65T variant of Aequorea victoria green fluorescent
                     protein (Heim et al., 1995)"
     CDS             3805..4200
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label="BSD"
                     /note="confers resistance to blasticidin"
                     /translation="AKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTGV
                     NVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGIK
                     AIVKDSDGQPTAVGIRELLPSGYVWEG"
     CDS             complement(4351..5343)
                     /codon_start=1
                     /locus_tag="PSW2_2"
                     /product="virulence integrase pGP8-D"
                     /label="PSW2_2"
                     /protein_id="AHD24925.1"
                     /translation="MGKGILSLQQEMSLEYSEKSYQEVLKIRQESYWKRMKSFSLFEVI
                     MHWTASLNKHTCRSYRGSFLSLEKIGLLSLDMNLQEFSLLNHNLILDAIKKVSSAKTSW
                     TEGTKQVRAASYISLTRFLNRMTQGIVAIAQPSKQENSRTFFKTREIVKTDAMNSLQTA
                     SFLKELKKINARDWLIAQTMLQGGKRSSEVLSLEISQICFQQATISFSQLKNRQTEKRI
                     IITYPQKFMHFLQEYIGQRRGFVFVTRSGKMVGLRQIARTFSQAGLQAAIPFKITPHVL
                     RATAVTEYKRLGCSDSDIMKVTGHATAKMIFAYDKSSREDNASKKLALI"
     gene            complement(4351..5343)
                     /locus_tag="PSW2_2"
                     /label="PSW2_2"
     CDS             5486..6838
                     /note="Probable plasmid replicative DNA helicase from
                     Chlamydia trachomatis serovar L2 (strain 434/Bu / ATCC
                     VR-902B). Accession#: B0BCM3"
                     /label="Probable plasmid replicative DNA helicase"
     CDS             6835..7899
                     /codon_start=1
                     /locus_tag="PSW2_4"
                     /product="putative virulence protein pGP2-D"
                     /label="PSW2_4"
                     /protein_id="AHD24927.1"
                     /translation="MVNYSNCHFIKSPIHLENQKFGRRPGQSIKISPKLAQNGMVEVIG
                     LDFLSSHYHALAAIQRLLTATNYKGNTKGVVLSRESNSFQFEGWIPRIRFTKTEFLEAY
                     GVKRYKTSRNKYEFSGKEAETALEALYHLGHQPFLIVATRTRWTNGTQIVDRYQTLSPI
                     IRIYEGWEGLTDEENIDIDLTPFNSPPTRKHKGFVVEPCPILVDQIESYFVIKPANVYQ
                     EIKMRFPNASKYAYTFIDWVITAAAKKRRKLTKDNSWPENLLLNVNVKSLAYILRMNRY
                     ICTRNWKKIELAIDKCIEIAIKLGWLSRRKRIEFLDSSKLSKKEILYLNKERFEEITKK
                     SKEQMEQLEQESIN"
     gene            6835..7899
                     /locus_tag="PSW2_4"
                     /label="PSW2_4"
     CDS             7961..8755
                     /codon_start=1
                     /locus_tag="PSW2_5"
                     /product="putative virulence protein pGP3-D"
                     /label="PSW2_5"
                     /protein_id="AHD24928.1"
                     /translation="MGNSGFYLYNTENCVFADNIKVGQMTEPLKDQQIILGTTSTPVAA
                     KMTASDGISLTVSNNSSTNASITIGLDAEKAYQLILEKLGDQILDGIADTIVDSTVQDI
                     LDKIKTDPSLGLLKAFNNFPITNKIQCNGLFTPSNIETLLGGTEIGKFTVTPKSSGSMF
                     LVSADIIASRMEGGVVLALVREGDSKPCAISYGYSSGIPNLCSLRTSITNTGLTPTTYS
                     LRVGGLESGVVWVNALSNGNDILGITNTSNVSFLEVIPQTNA"
     gene            7961..8755
                     /locus_tag="PSW2_5"
                     /label="PSW2_5"
     CDS             8825..9133
                     /codon_start=1
                     /locus_tag="PSW2_6"
                     /product="putative virulence protein pGP4-D"
                     /label="PSW2_6"
                     /protein_id="AHD24929.1"
                     /translation="MQNKRKVRDDFIKIVKDVKKDFPELDLKIRVNKEKVTFLNSPLEL
                     YHKSVSLILGLLQQIENSLGLFPDSPVLEKLEDNSLKLKKALIMLILSRKDMFSKAE"
     gene            8825..9133
                     /locus_tag="PSW2_6"
                     /label="PSW2_6"
     CDS             9161..9955
                     /codon_start=1
                     /locus_tag="PSW2_7"
                     /product="putative ParA family partitioning protein pGP5-D"
                     /label="PSW2_7"
                     /protein_id="AHD24930.1"
                     /translation="MHTLVFCSFKGGTGKTTLSLNVGCNLAQFLGKKVLLADLDPQSNL
                     SSGLGASVRSDQKGLHDIVYTSNDLKSIICETKKDSVDLIPASFSSEQFRELDIHRGPS
                     NNLKLFLNEYCAPFYDICIIDTPPSLGGLTKEAFVAGDKLIACLTPEPFSILGLQKIRE
                     FLSSVGKPEEEHILGIALSFWDDRNSTNQMYIDIIESIYKNKLFSTKIRRDISLSRSLL
                     KEDSVANVYPNSRAAEDILKLTHEIANILHIEYERDYSQRTT"
     gene            9161..9955
                     /locus_tag="PSW2_7"
                     /label="PSW2_7"
     CDS             9952..10695
                     /codon_start=1
                     /locus_tag="PSW2_8"
                     /product="putative virulence protein pGP6-D"
                     /label="PSW2_8"
                     /protein_id="AHD24931.1"
                     /translation="MNKLKKEADVFFKKNQTAASLDFKKTLPSIELFSATLNSEESQSL
                     DRLFLSESQNYSDEEFYQEDILAVKLLTGQIKSIQKQHVLLLGEKIYNARKILSKDHFS
                     STTFSSWIELVFRTKSSAYNALAYYELFINLPNQTLQKEFQSIPYKSAYILAARKGDLK
                     TKVDVIGKVCGMSNSSAIRVLDQFLPSSRNKDVRETIDKSDSEKNRQLSDFLIEILRIM
                     CSGVSLSSYNENLLQQLFELFKQKS"
     gene            9952..10695
                     /locus_tag="PSW2_8"
                     /label="PSW2_8"
ORIGIN
        1 tttgcaactc ttggtggtag actttgcaac tcttggtggt agactttgca actcttggtg
       61 gtagactttg caactcttgg tggtagactt ggtcataatg gacttttgtt aaaaaatttc
      121 ttaaaatctt agagctccga ttttgaatag ctttggttaa gaaaatgggc tcgatggctt
      181 tccataaaag tagattgttt ttaacttttg gggacgcgtc ggaaatttgg ttatctactt
      241 tatcttatct aactagaaaa aattatgcgt ctgggattaa ctttcttgtt tctttagaga
      301 ttctggattt atcggaaacc ttgataaagg ctatttctct tgaccacagc gaatctttgt
      361 ttaaaatcaa gtctctagat gtttttaatg gaaaagttgt ttcagaggca tctaaacagg
      421 ctagagcggc atgctacata tctttcacaa agtttttgta tagattgacc aagggatata
      481 ttaaacccgc tattccattg aaagattttg gaaacactac attttttaaa atccgagaca
      541 aaatcaaaac agaatcgatt tctaagcagg aatggacagt tttttttgaa gcgctccgga
      601 tagtgaatta tagagactat ttaatcggta aattgattgt acaagggatc cccgggtacc
      661 gagctcgaat tcatcgatga tatcagatct ggttctatag tgtcacctaa atcgtatgtg
      721 tatgatacat aaggttatgt attaattgta gccgcgttct aacgacaata tgtccatatg
      781 gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagcccc gacacccgcc
      841 aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc
      901 tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc
      961 gagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt
     1021 ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt
     1081 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca
     1141 ataatattga aaaaggaaga gtatggccaa gttgaccagt gccgttccgg tgctcaccgc
     1201 gcgcgacgtc gccggagcgg tcgagttctg gaccgaccgg ctcgggttct cccgggactt
     1261 cgtggaggac gacttcgccg gtgtggtccg ggacgacgtg accctgttca tcagcgcggt
     1321 ccaggaccag gtggtgccgg acaacaccct ggcctgggtg tgggtgcgcg gcctggacga
     1381 gctgtacgcc gagtggtcgg aggtcgtgtc cacgaacttc cgggacgcct ccgggccggc
     1441 catgaccgag atcggcgagc agccgtgggg gcgggagttc gccctgcgcg acccggccgg
     1501 caactgcgtg cactttgtgg cagaggagca ggactgactg tcagaccaag tttactcata
     1561 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct
     1621 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
     1681 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
     1741 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     1801 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct
     1861 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     1921 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
     1981 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     2041 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
     2101 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
     2161 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
     2221 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
     2281 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
     2341 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac
     2401 cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt
     2461 gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat
     2521 tcattaatgc aggttaacct ggcttatcga aattaatacg actcactata gggagaccgg
     2581 cctcgagcag ctgaagcttg catgcctgca gatgcccgac ggtctttata gcggattaac
     2641 aaaaatcagg acaaggcggc gaagccgaag acagtacaaa tagcacggaa ccgattcact
     2701 tggtgcttca gcaccttaga gaatcgttct ctttgagcta aggcgaggca acgccgtact
     2761 tgtttttgtt aatccactat aaagtgccgc gtgtgttttt ttatggcgtt ttaaaaagcc
     2821 gagactgcat ccgggcagca gcgcatcggc ccgcacgagg tctgcgcttg aattgtgttg
     2881 tagaaacaca acgttttttg aaaaaataag ctattgtttt atatcaaaat ataatcattt
     2941 ttaaaataaa ggttgcggca tttatcagat atttgttctg aaaaatggtt ttttgcgggg
     3001 ggggggtata attgaagacg tatcgggtgt ttgcccgatg tttttaggtt tttatcaaat
     3061 ttacaaaagg aagccgatat ggtggatccc cgggtaccaa tgagtaaagg agaagcactt
     3121 ttcactggag ttgtcccaat tcttgttgaa ttagatggtg atgttaatgg gcacaaattt
     3181 tctgtcagtg gagagggtga aggtgatgca acatacggaa aacttaccct taaatttatt
     3241 tgcactactg gaaaactacc tgttccatgg ccaacacttg tcactactct tacgtatggt
     3301 gttcaatgct tttcaagata cccagatcat atgaaacggc atgacttttt caagagtgcc
     3361 atgcccgaag gttatgtaca ggaaagaact atatttttca aagatgacgg gaactacaag
     3421 acacgtgctg aagtcaagtt tgaaggtgat acccttgtta atagaatcga gttaaaaggt
     3481 attgatttta aagaagatgg aaacattctt ggacacaaat tggaatacaa ctataactca
     3541 cacaatgtat acatcatggc agacaaacaa aagaatggaa tcaaagttaa cttcaaaatt
     3601 agacacaaca ttgaagatgg aagcgttcaa ctagcagacc attatcaaca aaatactcca
     3661 attggcgatg gccctgtcct tttaccagac aaccattacc tgtccacaca atctgccctt
     3721 tcgaaagatc ccaacgaaaa gagagaccac atggtccttc ttgagtttgt aacagctgct
     3781 gggattacac atggcatgga tcaggccaag cctttgtctc aagaagaatc caccctcatt
     3841 gaaagagcaa cggctacaat caacagcatc cccatctctg aagactacag cgtcgccagc
     3901 gcagctctct ctagcgacgg ccgcatcttc actggtgtca atgtatatca ttttactggg
     3961 ggaccttgtg cagaactcgt ggtgctgggc actgctgctg ctgcggcagc tggcaacctg
     4021 acttgtatcg tcgcgatcgg aaatgagaac aggggcatct tgagcccctg cggacggtgc
     4081 cgacaggtgc ttctcgatct gcatcctggg atcaaagcca tagtgaagga cagtgatgga
     4141 cagccgacgg cagttgggat tcgtgaattg ctgccctctg gttatgtgtg ggagggctaa
     4201 agatctcgag ctcgatatct agattaatgt cgactctaga ggatccgttt gttctgggga
     4261 agaggtaatt cctctagtac aaacacccac aatattgtga tataattaaa attatattca
     4321 tattctgttg ccagaaaaaa cacctttagg ctatattaga gccagcttct ttgaagcgtt
     4381 gtcttctcga gaagatttat cgtacgcaaa tatcatcttt gcggttgcgt gtcctgtgac
     4441 cttcattatg tcggagtctg agcaccctag gcgtttgtac tccgtcacag cggttgctcg
     4501 aagcacgtgc ggggttattt taaaagggat tgcagcttgt agtcctgctt gagagaacgt
     4561 gcgggcgatt tgccttaacc ccaccatttt tccggagcga gttacgaaga caaaacctct
     4621 tcgttgaccg atgtactctt gtagaaagtg cataaacttc tgaggataag ttataataat
     4681 cctcttttct gtctgacggt tcttaagctg ggagaaagaa atggtagctt gttggaaaca
     4741 aatctgacta atctccaagc ttaagacttc agaggagcgt ttacctcctt ggagcattgt
     4801 ctgggcgatc aaccaatccc gggcattgat tttttttagc tcttttagga aggatgctgt
     4861 ttgcaaactg ttcatcgcat ccgtttttac tatttccctg gttttaaaaa atgttcgact
     4921 attttcttgt ttagaaggtt gcgctatagc gactattcct tgagtcatcc tgtttaggaa
     4981 tcttgttaag gaaatatagc ttgctgctcg aacttgttta gtaccttcgg tccaagaagt
     5041 cttggcagag gaaacttttt taatcgcatc taggattaga ttatgattta aaagggaaaa
     5101 ctcttgcaga ttcatatcca aggacaatag accaatcttt tctaaagaca aaaaagatcc
     5161 tcgatatgat ctacaagtat gtttgttgag tgatgcggtc caatgcataa taacttcgaa
     5221 taaggagaag cttttcatgc gtttccaata ggattcttgg cgaattttta aaacttcctg
     5281 ataagacttt tcactatatt ctaacgacat ttcttgctgc aaagataaaa tccctttacc
     5341 catgaaatcc ctcgtgatat aacctatccg taaaatgtcc tgattagtga aataatcagg
     5401 ttgttaacag gatagcacgc tcggtatttt tttatataaa caggttgtta acaggatagc
     5461 acgctcggta tttttttata taaacatgaa aactcgttcc gaaatagaaa atcgcatgca
     5521 agatatcgag tatgcgttgt taggtaaagc tctgatattt gaagactcta ctgagtatat
     5581 tctgaggcag cttgctaatt atgagtttaa gtgttctcat cataaaaaca tattcatagt
     5641 atttaaatac ttaaaagaca atggattacc tataactgta gactcggctt gggaagagct
     5701 tttgcggcgt cgtatcaaag atatggacaa atcgtatctc gggttaatgt tgcatgatgc
     5761 tttatcaaat gacaagctta gatccgtttc tcatacggtt ttcctcgatg atttgagcgt
     5821 gtgtagcgct gaagaaaatt tgagtaattt cattttccgc tcgtttaatg agtacaatga
     5881 aaatccattg cgtagatctc cgtttctatt gcttgagcgt ataaagggaa ggcttgatag
     5941 tgctatagca aagacttttt ctattcgcag cgctagaggc cggtctattt atgatatatt
     6001 ctcacagtca gaaattggag tgctggctcg tataaaaaaa agacgagtag cgttctctga
     6061 gaatcaaaat tctttctttg atggcttccc aacaggatac aaggatattg atgataaagg
     6121 agttatctta gctaaaggta atttcgtgat tatagcagct agaccatcta tagggaaaac
     6181 agctttagct atagacatgg cgataaatct tgcggttact caacagcgta gagttggttt
     6241 cctatctcta gaaatgagcg caggtcaaat tgttgagcgg attattgcta atttaacagg
     6301 aatatctggt gaaaaattac aaagagggga tctctctaaa gaagaattat tccgagtaga
     6361 agaagctgga gaaacggtta gagaatcaca tttttatatc tgcagtgata gtcagtataa
     6421 gcttaactta atcgcgaatc agatccggtt gctgagaaaa gaagatcgag tagacgtaat
     6481 atttatcgat tacttgcagt tgatcaactc atcggttgga gaaaatcgtc aaaatgaaat
     6541 agcagatata tctagaacct taagaggttt agcctcagag ctaaacattc ctatagtttg
     6601 tttatcccaa ctatctagaa aagttgagga tagagcaaat aaagttccca tgctttcaga
     6661 tttgcgagac agcggtcaaa tagagcaaga cgcagatgtg attttgttta tcaataggaa
     6721 ggaatcgtct tctaattgtg agataactgt tgggaaaaat agacatggat cggttttctc
     6781 ttcggtatta catttcgatc caaaaattag taaattctcc gctattaaaa aagtatggta
     6841 aattatagta actgccactt catcaaaagt cctatccacc ttgaaaatca gaagtttgga
     6901 agaagacctg gtcaatctat taagatatct cccaaattgg ctcaaaatgg gatggtagaa
     6961 gttataggtc ttgattttct ttcatctcat taccatgcat tagcagctat ccaaagatta
     7021 ctgaccgcaa cgaattacaa ggggaacaca aaaggggttg ttttatccag agaatcaaat
     7081 agttttcaat ttgaaggatg gataccaaga atccgtttta caaaaactga attcttagag
     7141 gcttatggag ttaagcggta taaaacatcc agaaataagt atgagtttag tggaaaagaa
     7201 gctgaaactg ctttagaagc cttataccat ttaggacatc aaccgttttt aatagtggca
     7261 actagaactc gatggactaa tggaacacaa atagtagacc gttaccaaac tctttctccg
     7321 atcattagga tttacgaagg atgggaaggt ttaactgacg aagaaaatat agatatagac
     7381 ttaacacctt ttaattcacc acctacacgg aaacataaag ggttcgttgt agagccatgt
     7441 cctatcttgg tagatcaaat agaatcctac tttgtaatca agcctgcaaa tgtataccaa
     7501 gaaataaaaa tgcgtttccc aaatgcatca aagtatgctt acacatttat cgactgggtg
     7561 attacagcag ctgcgaaaaa gagacgaaaa ttaactaagg ataattcttg gccagaaaac
     7621 ttgttattaa acgttaacgt taaaagtctt gcatatattt taaggatgaa tcggtacatc
     7681 tgtacaagga actggaaaaa aatcgagtta gctatcgata aatgtataga aatcgccatt
     7741 aagcttggct ggttatctag aagaaaacgc attgaatttc tggattcttc taaactctct
     7801 aaaaaagaaa ttctatatct aaataaagag cgctttgaag aaataactaa gaaatctaaa
     7861 gaacaaatgg aacaattaga acaagaatct attaattaat agcaagcttg aaactaaaaa
     7921 cctaatttat ttaaagctca aaataaaaaa gagttttaaa atgggaaatt ctggttttta
     7981 tttgtataac actgaaaact gcgtctttgc tgataatatc aaagttgggc aaatgacaga
     8041 gccgctcaag gaccagcaaa taatccttgg gacaacatca acacctgtcg cagccaaaat
     8101 gacagcttct gatggaatat ctttaacagt ctccaataat tcatcaacca atgcttctat
     8161 tacaattggt ttggatgcgg aaaaagctta ccagcttatt ctagaaaagt tgggagatca
     8221 aattcttgat ggaattgctg atactattgt tgatagtaca gtccaagata ttttagacaa
     8281 aatcaaaaca gacccttctc taggtttgtt gaaagctttt aacaactttc caatcactaa
     8341 taaaattcaa tgcaacgggt tattcactcc cagtaacatt gaaactttat taggaggaac
     8401 tgaaatagga aaattcacag tcacacccaa aagctctggg agcatgttct tagtctcagc
     8461 agatattatt gcatcaagaa tggaaggcgg cgttgttcta gctttggtac gagaaggtga
     8521 ttctaagccc tgcgcgatta gttatggata ctcatcaggc attcctaatt tatgtagtct
     8581 aagaaccagt attactaata caggattgac tccgacaacg tattcattac gtgtaggcgg
     8641 tttagaaagc ggtgtggtat gggttaatgc cctttctaat ggcaatgata ttttaggaat
     8701 aacaaatact tctaatgtat cttttttaga ggtaatacct caaacaaacg cttaaacaat
     8761 ttttattgga tttttcttat aggttttata tttagagaaa acagttcgaa ttacggggtt
     8821 tgttatgcaa aataaaagaa aagtgaggga cgattttatt aaaattgtta aagatgtgaa
     8881 aaaagatttc cccgaattag acctaaaaat acgagtaaac aaggaaaaag taactttctt
     8941 aaattctccc ttagaactct accataaaag tgtctcacta attctaggac tgcttcaaca
     9001 aatagaaaac tctttaggat tattcccaga ctctcctgtt cttgaaaaat tagaggataa
     9061 cagtttaaag ctaaaaaagg ctttgattat gcttatcttg tctagaaaag acatgttttc
     9121 caaggctgaa tagacaactt actctaacgt tggagttgat ttgcacacct tagttttttg
     9181 ctcttttaag ggaggaactg gaaaaacaac actttctcta aacgtgggat gcaacttggc
     9241 ccaattttta gggaaaaaag tgttacttgc tgacctagac ccgcaatcca atttatcttc
     9301 tggattgggg gctagtgtca gaagtgacca aaaaggcttg cacgacatag tatacacatc
     9361 aaacgattta aaatcaatca tttgcgaaac aaaaaaagat agtgtggacc taattcctgc
     9421 atcattttca tccgaacagt ttagagaatt ggatattcat agaggaccta gtaacaactt
     9481 aaagttattt ctgaatgagt actgcgctcc tttttatgac atctgcataa tagacactcc
     9541 acctagccta ggagggttaa cgaaagaagc ttttgttgca ggagacaaat taattgcttg
     9601 tttaactcca gaaccttttt ctattctagg gttacaaaag atacgtgaat tcttaagttc
     9661 ggtcggaaaa cctgaagaag aacacattct tggaatagct ttgtcttttt gggatgatcg
     9721 taactcgact aaccaaatgt atatagacat tatcgagtct atttacaaaa acaagctttt
     9781 ttcaacaaaa attcgtcgag atatttctct cagccgttct cttcttaaag aagattctgt
     9841 agctaatgtc tatccaaatt ctagggccgc agaagatatt ctgaagttaa cgcatgaaat
     9901 agcaaatatt ttgcatatcg aatatgaacg agattactct cagaggacaa cgtgaacaaa
     9961 ctaaaaaaag aagcggatgt cttttttaaa aaaaatcaaa ctgccgcttc tctagatttt
    10021 aagaagacgc ttccctccat tgaactattc tcagcaactt tgaattctga ggaaagtcag
    10081 agtttggatc gattattttt atcagagtcc caaaactatt cggatgaaga attttatcaa
    10141 gaagacatcc tagcggtaaa actgcttact ggtcagataa aatccataca gaagcaacac
    10201 gtacttcttt taggagaaaa aatctataat gctagaaaaa tcctgagtaa ggatcacttc
    10261 tcctcaacaa ctttttcatc ttggatagag ttagttttta gaactaagtc ttctgcttac
    10321 aatgctcttg catattacga gctttttata aacctcccca accaaactct acaaaaagag
    10381 tttcaatcga tcccctataa atccgcatat attttggccg ctagaaaagg cgatttaaaa
    10441 accaaggtcg atgtgatagg gaaagtatgt ggaatgtcga actcatcggc gataagggtg
    10501 ttggatcaat ttcttccttc atctagaaac aaagacgtta gagaaacgat agataagtct
    10561 gattcagaga agaatcgcca attatctgat ttcttaatag agatacttcg catcatgtgt
    10621 tccggagttt ctttgtcctc ctataacgaa aatcttctac aacagctttt tgaacttttt
    10681 aagcaaaaga gctgatcctc cgtcagctca tatatatata tctattatat atatatattt
    10741 agggatttga tttcacgaga gaga
//
